Labshake search
Citations for Bio-Rad :
151 - 200 of 326 citations for Recombinant Human Fc Fragment Of LgG Receptor Transporter Alpha since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
-
bioRxiv - Immunology 2024Quote: 96-well flat-bottom NUNC Maxisorp plates were coated overnight at 4 °C with 0.5 mg/mL recombinant HBsAg (BIO-RAD). Plates were washed with PBS/T ...
-
bioRxiv - Microbiology 2022Quote: ... from 22 people were quantified using a Bio-Plex Pro™ Human Inflammation 24-Plex Panel or a Bio-Plex Pro™ Human Th17 Cytokine Panel 15-Plex Panel (Bio-Rad, Hercules, CA, USA). Cytokines were omitted from further analyses if their concentration was close to the lower limit of detection in most samples ...
-
bioRxiv - Biochemistry 2021Quote: ... Human primers were designed by using Beacon Designer software (Bio-Rad). Quantitative PCR was performed on a CFX96 real-time PCR thermocycler (Bio-Rad ...
-
bioRxiv - Immunology 2019Quote: The following antibodies were used: anti-human CD53 (MEM-53, Biorad), anti-human CD53-488 (MEM-53 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Bio-Plex Pro Human Cytokine VCAM-1 (BioRad, USA; 171B6022M) were used ...
-
bioRxiv - Immunology 2020Quote: Human CTL were transfected using a GenePulser Xcell electroporation system (BioRad). 1x106 CTL (5days after restimulation therefore in expansion phase ...
-
bioRxiv - Pathology 2023Quote: ... or mouse monoclonal antibodies against human C3d (Bio-Rad Laboratories, CA) and C4d (Santa Cruz ...
-
bioRxiv - Neuroscience 2024Quote: ... Primers/probes to detect human RPP30 (BioRad Custom Assay, Hercules, CA) were used as an endogenous reference in human samples ...
-
bioRxiv - Plant Biology 2019Quote: ... Adapter-ligated fragments ranging from 200 to 300 nucleotides were isolated by agarose gel electrophoresis (Bio-Rad, Hercules, CA) and purified using the MinElute Gel Extraction Kit (Qiagen ...
-
bioRxiv - Genetics 2021Quote: ... enrichment relative to an input of immunoprecipitated chromatin fragments was determined using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on the LightCycler480 (Roche ...
-
bioRxiv - Microbiology 2022Quote: ... Size separation of restricted DNA fragments was performed using a CHEF MAPPER XA system (Bio-Rad, Richmond, CA, USA) with 1% SeaKem gold agarose gel for 21 h ...
-
bioRxiv - Microbiology 2021Quote: ... The Ptat promoter DNA fragment was obtained by PCR using the iProof High-Fidelity DNA Polymerase (Bio-Rad, USA) and PA154197 genomic DNA as the template ...
-
bioRxiv - Microbiology 2023Quote: ... GFP-A51R truncations were created by PCR amplification of fragments from A51R templates using iProof DNA Polymerase (Bio-Rad) and cloned into the pGFP-C3 vector ...
-
bioRxiv - Genetics 2023Quote: ... Pulled-down DNA fragments were analyzed via qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. No. 1725121), primers are shown in Supplementary Information.
-
bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
-
bioRxiv - Microbiology 2019Quote: SDS-PAGE analysis of the recombinant TMPT2A (rTMPT2A) was performed on linear 12.5% polyacrylamide minigels in a Mini-Protean® Tetra cell system (BioRad, USA), as described by [22] ...
-
bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
-
bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
-
bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
-
bioRxiv - Biochemistry 2023Quote: ... and the fractions containing the recombinant vaccine candidates were automatically collected using BioFrac™ Fraction Collector (Bio-Rad Laboratories, Hercules, USA) when the 280 nm absorbance was greater than 0.05 ...
-
bioRxiv - Immunology 2022Quote: ... Pre-designed human gene primers were purchased from Bio-Rad (supplemental Table). Human PPIA was amplified in parallel and used as the reference gene in quantification ...
-
bioRxiv - Biochemistry 2020Quote: ... with goat anti-human IgG F(ab’)2-HRP (STAR126P, Bio-Rad) as the detection antibody.
-
bioRxiv - Biophysics 2020Quote: ... and mouse anti-human CD34 (QBEND 10 clone, Ms IgG1, Bio-Rad). Briefly ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human primers for HLA-DRB1 and VCAM1 were purchased from Bio-Rad.
-
bioRxiv - Immunology 2020Quote: ... and incubated with either FITC-conjugated Sheep Anti-Human C3 (BioRad AHP031F) at 1:500 or AF488-conjugated Mouse Anti-C5b-9 (ae11 ...
-
Antiviral roles of interferon regulatory factor (IRF)-1, 3 and 7 against human coronavirus infectionbioRxiv - Microbiology 2022Quote: ... human IFN-γ and IFN-λ 1 were obtained from Bio-Rad, BD Pharmingen and R&D Systems respectively ...
-
bioRxiv - Cancer Biology 2023Quote: ... and G-CSF quantification using a human Bio-plex PRO assay (Biorad) following the manufacturer’s instructions and acquired with the Luminex200 instrument (Luminex).
-
bioRxiv - Immunology 2023Quote: ... A human RPP30 copy number assay labeled with HEX (Bio-Rad, dHsaCP1000485) was used to measure total allelic copy numbers ...
-
bioRxiv - Immunology 2023Quote: ... with human IgA positive and negative controls (Bio-Rad, Cat No. 12014775), Bio-Plex SARS-CoV-2 Wuhan-1 strain RBD and N coupled beads (Bio-Rad ...
-
bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was obtained by incubating the samples (whole embryos or adult caudal fin fragments) in TE buffer supplemented with 5% Chelex-100 (BioRad) and 10 μg/ml Proteinase K (Roche ...
-
bioRxiv - Genomics 2020Quote: ... DNA fragments were separated by pulsed-field gel electrophoresis (PFGE) through a CHEF-DR III system (Bio-Rad, Hercules, USA). The plasmid DNA was transferred to a positively charged nylon membrane (Solabio ...
-
bioRxiv - Biochemistry 2020Quote: Cross-linked samples of purified ROS-GC1 fragment (aa 814-1110) were analyzed by SDS-PAGE using gradient gels (4-20%, Mini-PROTEAN TGX gels, Biorad). For in-gel digestion ...
-
bioRxiv - Microbiology 2022Quote: ... Ten microliters of each amplicon were separated on 2% agarose gel in 1X TAE buffer for 35 min at 135 volts and the amplified fragment detected using a GelDoc imager (BioRad).
-
bioRxiv - Microbiology 2023Quote: ... The amplified fragments were analysed by agarose gel electrophoresis and visualized by gel documentation system (GelDoc, Bio-Rad, Hercules, USA). The 1800 bp positive amplicons was purified using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation ...
-
bioRxiv - Cancer Biology 2023Quote: ... The relative abundance of genomic DNA fragments was determined using Sso Advanced Universal SYBR Green Supermix (Cat. #1725271, Bio-Rad) with the following primers specific for the promoter region of human ALPP gene.
-
bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
-
bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was determined by interpolation on to absorbance curves generated by recombinant protein standards using iMark™ Microplate Absorbance Reader (Bio-Rad).
-
bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
-
bioRxiv - Microbiology 2019Quote: DNA fragments that co-precipitated with H-NSHA were quantified by real-time quantitative PCR using SsoFast EvaGreen Supermix (Bio-Rad) according to the manufacturer’s instructions and using gene-specific primers ...
-
bioRxiv - Plant Biology 2019Quote: ... Libraries were checked for appropriate fragment sizes (<500bp) and distribution on an Experion automatic electrophoresis system (Bio-Rad, Laboratories, CA, USA). The enzyme EcoT22I ...
-
bioRxiv - Microbiology 2022Quote: ... and probed in triplicate by qPCR for the presence of the 1 kb capsule fragment using iTaq DNA polymerase (BIO-RAD) and primer pair DDL34-DDL35 ...
-
bioRxiv - Pathology 2023Quote: ... and DNA fragment (amplification products containing homologous DNA fragments) in a 4-mm Gene Pulser and electrotransformed by Gene Pulser Xcell system (Bio-Rad). Transgenic parasites were selected with pyrimidine (1μM ...
-
bioRxiv - Plant Biology 2024Quote: pTRV1 and pTRV2-Del/Ros-1 harboring the SlATG7- or SlATG2-derived fragments were introduced into Agrobacterium strain GV3101 using electroporation (BIO-RAD). A 5 mL culture was grown overnight at 28°C in a medium containing kanamycin ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 50 μl of competent cells were mixed with 500 ng of the PCR fragment in an ice-cold 0.2 cm cuvette (Bio-Rad Inc.). Cells were electroporated at 2.5 kV with 25 mF and 200 Ω ...
-
bioRxiv - Cell Biology 2024Quote: ... Fragments amplified using the FastGene Optima HotStart Ready Mix (Nippon Genetics) over 35 cycles in a C1000 Thermal Cycler (Bio-Rad) were subjected to agarose gel electrophoresis ...
-
bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
-
bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
-
bioRxiv - Microbiology 2022Quote: ... and Bio-Plex Pro Human Cytokine 27-plex assay (Bio-Rad, California, USA) were used to detect urinary cytokines ...