Labshake search
Citations for Bio-Rad :
151 - 200 of 4793 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... RNA electrophoresis was performed at 200 V for 1 h and then RNA was transferred to a nylon membrane using MiniProtean 3 system (BioRad, USA). Transfer conditions were 100 V for 1 h at 4 °C in TBE buffer 1 × ...
-
bioRxiv - Microbiology 2022Quote: ... Beads were washed 3 times in PBS and eluted in 40 μL 2X Laemmli Buffer with 1:20 β-mercapto-ethanol (BioRad) at 95°C for 5 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... then incubated with ProSignal Pico Spray chemiluminescence substrate (Prometheus) for 1-3 minutes at room temperature and imaged using a ChemiDoc Imaging System (Bio-Rad). Contrast was occasionally adjusted to improve visualization of bands ...
-
bioRxiv - Genomics 2022Quote: ... and incubated at 65°C for 3 mins before running 1% agarose-Etbr gel electrophoresis to detect the cleavage efficiency by ChemiDoc system (Bio-Rad).
-
Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
-
bioRxiv - Immunology 2024Quote: ... After final wash and supernatant removal, 1x Loading Buffer (40 μL, 3:1 Lysis Buffer:4x Laemmli Loading Buffer (Bio-Rad)) was added to the sample and the sample boiled (10 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Blocking solution consisted in 3% nonfat dry milk (Blotting-Grade Blocker, BIO-RAD Laboratories ...
-
bioRxiv - Plant Biology 2021Quote: ... 3 μL cDNA template and 4 μL iQ SYBR green supermix (Bio-Rad) in a total reaction volume of 12 μL ...
-
bioRxiv - Cell Biology 2021Quote: ... proteins were separated on a 3-8% Criterion XT tris-acetate gel (Biorad) or 4-15% Criterion TGX gel according to the instructions of the manufacturer ...
-
bioRxiv - Microbiology 2021Quote: ... were resolved by 10% denaturing PAGE in Mini Protean 3 Cell (Bio-Rad). Separated RNA was then transferred to a nylon membrane (Hybond-XL ...
-
bioRxiv - Molecular Biology 2021Quote: ... with 3 uL Precision Plus Protein All Blue prestained standards (Bio-Rad, #1610373) loaded as a ladder ...
-
TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... or 3-8% Tris-Acetate CriterionTM XT Precast Gels (Bio-Rad Laboratories, Invitrogen) at 150 V for 90 min ...
-
bioRxiv - Genomics 2022Quote: ... and 3 μl of 2x iProof HF Master Mix (BioRad, Hercules, CA, USA). The 3 μl of 2x master mix was dispensed using a Mantis Liquid Handler (Formulatrix ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3 µl DTT and 1000 µl Purezol (Biorad, USA, CA, Cat No: 7326890) were added to each falcon tube and kept on ice for 5 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... on an Opticon 2 from MJ Research with Opticon Monitor 3 software (BioRad). Reactions were set up manually using an 8-channel pipette (30-300μL ...
-
bioRxiv - Bioengineering 2024Quote: 3⋅108 total particles were loaded into pre-cast gels (BioRad, Cat. # 4561094). Samples were blocked in a 5% nonfat milk solution and stained for the common EV marker CD9 using a primary antibody (Cell Signaling Technologies ...
-
bioRxiv - Bioengineering 2024Quote: ... and Precision Plus Protein Dual Color Standards (1610374, Bio-Rad, 3 μL/well). All antibodies used for western blotting were diluted at a ratio of 1:1000 or 1:500 unless otherwise specified ...
-
bioRxiv - Cancer Biology 2024Quote: ... and Bio-Plex Pro™ Mouse TGF-β 3-Plex assays (Bio-Rad) were utilized to profile the concentrations of mouse cytokines and chemokines in the cell culture supernatants ...
-
bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
-
bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
-
bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
-
bioRxiv - Genomics 2020Quote: Lysates were separated by SDS-PAGE on 3-8% Tris-acetate gels (BioRad #3450129) for 2.5 hours at 150V ...
-
bioRxiv - Biophysics 2021Quote: ... Detergents were removed by adding 2-3 batches of Bio-beads SM2 (Bio-Rad) with constant rotation for overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 3 µl was used for qPCR with iTaq Universal SYBR Green Supermix (BioRad). Relative levels of RNA were calculated by the ΔΔCt method using GAPDH as an internal control ...
-
bioRxiv - Microbiology 2021Quote: ... The nitrocellulose membrane was soaked (blocked) in 3% milk (dry milk from Bio-Rad) solution in 1x PBS following by incubation with appropriate primary antibody solution (1:1000 dilution ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... washed 3 times and developed with ECL reagent (Bio-Rad Laboratories, Hercules, CA, USA). Invertase blots were prepared similarly with an anti-invertase antibody and anti-goat IgG HRP ...
-
bioRxiv - Molecular Biology 2021Quote: ... SDS-PAGE and Western blotting were performed using the Mini-PROTEAN 3 system (Biorad). Blotted membranes were blocked in 5% BSA / TBS-Tween ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... kinase-dead extracts were incubated 3 consecutive times with protein A-beads (Bio-Rad) previously coated with the anti-B55δ antibody (1 hour incubation at 4°C ...
-
bioRxiv - Physiology 2023Quote: ... Blotting membrane was primed for 3 min prior in cold transfer buffer (BIORAD 10026938), according to manufacturer specifications ...
-
bioRxiv - Developmental Biology 2023Quote: ... Following separation via SDS-PAGE (12%, Mini PROTEAN 3 System, Bio-Rad, Hercules, CA), proteins were transferred to PVDF membrane for 1hr using a Genie electroblot chamber (Idea Scientific ...
-
bioRxiv - Immunology 2024Quote: ... Samples were diluted in Laemmli sample loading buffer supplemented with |3-mercaptoethanol (Bio-Rad), heated for 5 min at 95°C ...
-
bioRxiv - Immunology 2023Quote: ... 3) live cell populations using live-dead exclusion dye (Zombie Aqua, #423101, Bio-Rad); 4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE and Western blotting were performed using the Mini-PROTEAN 3 system (Biorad). Blotted membranes were blocked in 5% BSA / TBS-Tween ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3 μg of the protein samples were prepared with 4X Laemmli loading buffer (Biorad) and denatured at 95°C for 5 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... samples were run through Criterion XT Tris-acetate precast gels (3-8% gradient) (Biorad) in a XT tricine buffer (Biorad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Reactions were loaded onto a 3% agarose gel and visualized on a Chemidoc (BioRad). Bands were densitometry quantified by ImageJ and the percentage of methylated DNA was calculated over the total intensity of all the quantified bands.
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed 3 times with TBST before exposure using a Chemidoc system (BioRad).
-
bioRxiv - Immunology 2024Quote: ... and hFAB rhodamine anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (catalog number 12004168; Bio-Rad) (1:10,000).
-
bioRxiv - Microbiology 2024Quote: ... then 3 μL from each well was spotted onto a nitrocellulose membrane (Bio-Rad) and allowed to dry for at least 30 minutes in a fume hood at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... the DDM was removed by 3 additions of SM-2 bio-beads (Bio-Rad), incubated for 2h/2h/overnight ...
-
bioRxiv - Bioengineering 2024Quote: ... and 3 mM MgCl2) on a CFX96 thermocycler with an optical module (Bio-Rad) by the following protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
-
bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer: 2% Blotting Grade Blocker (Bio-Rad cat. # 1706404) and 2% heat-inactivated goat serum (Gibco ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein concentration of VSW-3 RNAP was determined by Bradford protein quantitative kit (Bio-rad), and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs ...
-
bioRxiv - Immunology 2020Quote: ... Development was performed after 3 washes in TBS-T and using Clarity ECL reagents (BioRad) and automatic exposure set up on ChemiDoc imaging system (BioRad).
-
bioRxiv - Microbiology 2020Quote: ... The membrane was then washed 3 times in TBST and once in TBS (Bio-rad) before being exposed to 2ml of Western Lightning Plus-ECL ...
-
bioRxiv - Plant Biology 2022Quote: ... The membrane was washed 3 times before image acquisition by ChemiDoc Touch Imaging System (Biorad). sRNA libraries were constructed with QIAgen miRNA library (QIAgen #331502 ...