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Citations for Bio-Rad :
1901 - 1950 of 2823 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... Quantitative PCR was performed in 10 µl reaction containing 5 µl SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µl H20 ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were separated on 10% or 15% SDS-PAGE gels and transferred to nitrocellulose membrane (Bio-Rad). Membranes were incubated with the primary antibodies listed in Supplemental Table S2 overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and 5-10 μg aliquots fractionated by SDS-PAGE then transferred to nitrocellulose (0.45 μm) membrane (Bio-Rad). Membranes were blocked for 1 h at room temperature in 5% BSA in 1x Tris-buffered saline + 0.1% Tween 20 (TBST) ...
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bioRxiv - Neuroscience 2023Quote: ... Glycoproteins were eluted by boiling at 100°C for 10 minutes using 4x Laemmli sample buffer (Bio-Rad). Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were run on a 10 % SDS-PAGE gel and transferred to a polyvinylidene difluoride membrane (Bio-Rad). Membranes were blocked with 5% milk (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... 10 µL of the supernatant was loaded in a polyacrylamide 4-15% Mini-PROTEAN precast gel (Bio-Rad) along with 5 µL of pre-stained protein ladder marker (ThermoScientific ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated in 10% (w/v) SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Bio-Rad). Membranes were blocked in 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... with 10% β-mercaptoethanol and subsequently run on a 4-20% Mini-PROTEAN TGX protein gel (Bio-Rad). After semi-dry transfer onto nitrocellulose membrane ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a 1 ml HisTrap (Cytiva) column connected to NGC Quest 10 (Bio-Rad), previously equilibrated with Buffer A (25 mM Tris–HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... qPCRs were performed in 10 μL in 96-well plates on a CFX OPUS-96 thermocycler (Bio-Rad) with 5 μL of 2X PerfeCTa SYBR Green Supermix (Quantabio) ...
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bioRxiv - Developmental Biology 2024Quote: ... RT-PCR was performed on 1:10 cDNA dilutions using Sso Fast EvaGreen supermix (Bio-Rad, 172-5280) on a thermocycler (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... samples were boiled (10 min) and loaded (20 µL) on 15-well 4-20 % acrylamide precast gradient gels (Biorad). Supernatant-alone samples were similarly boiled and loaded on a cast 10% acrylamide gel ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3 times for 10 minutes each and coated with enhanced chemiluminescence (ECL; Clarity ECL, BioRad). Using X-ray film ...
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bioRxiv - Cancer Biology 2021Quote: ... For WB the beads were boiled for 10 minutes in 1X Laemmli Sample Buffer (Bio-Rad, Cat. No. 1610737) containing 2- mercaptoethanol ...
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bioRxiv - Developmental Biology 2020Quote: ... These cell extracts were run on a 10% acrylamide gel before being transferred to a PVDF membrane (Bio-Rad). The membranes were blocked in 5% milk prior to incubation with primary antibodies overnight at 4°C ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... Fifty micrograms of protein from each group was separated on 10% Tris-HCl gel using protein electrophoresis (BioRad, USA). The gels were stained in Coomassie brilliant blue overnight (o/n) ...
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bioRxiv - Bioengineering 2020Quote: The cDNA was diluted 1/5 with dH2O and 2.5 µL were used for qPCR analysis (10 µL total volume; BioRad, iTaq Universal SYBR Green Supermix ...
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bioRxiv - Plant Biology 2019Quote: ... Total proteins (40 µg) were separated using SDS-PAGE (10 % acrylamide) and then blotted onto nitrocellulose membranes (1620112, Biorad). Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were resolved by SDS-PAGE on 10% polyacrylamide gels and transferred to nitrocellulose (Bio-Rad, catalog number 1620167). Primary antibodies used were mouse anti-β-actin monoclonal antibody (Sigma ...
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bioRxiv - Microbiology 2019Quote: 2 μl of serially diluted cDNA samples was mixed with 10 μl of 2× ddPCR supermix for probes (BioRad), 1 μl of 20× WT primers/probe ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in approximately 10 μl M9 medium and spotted onto 1% low-fluorescence agarose pads (Bio-Rad) prepared in M9 medium and covered with a no1.5 glass coverslip ...
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed in 20 μL reactions in triplicate containing 10 μL 2 × SsoFast EvaGreen Supermix (Bio-Rad,), 1 μL of primers (3 μM F and 3 μM R) ...
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of total protein underwent separation by 10% SDS-PAGE and electro-transfer on nitrocellulose membranes (Bio-Rad). After blocking (5% skim milk in Tris-buffered saline with 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2021Quote: ... were surrounded with a hydrophobic pen and permeabilized and blocked with 10% normal donkey serum (NDS; Bio-Rad, USA)/ 0.1% Triton X-100 (Carl Roth ...
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bioRxiv - Neuroscience 2021Quote: ... 10-20 µg of total protein was loaded on precast SDS-PAGE gels (4-20% Criterion TGX, Bio-rad) and immobilon-FL membranes (Merck Milipore ...
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bioRxiv - Cancer Biology 2020Quote: ... 400 nM of each primer and 250 nM of probe and 10 µl of IQ Supermixe for QPCR (Biorad). The reaction was performed as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... 300 nM of each primer and 100 nM of probe and 10 µl of IQ Supermixe for qPCR (Biorad). Each DNA solution was assayed in duplicate per qPCR assay ...
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bioRxiv - Cell Biology 2021Quote: ... The N-ter (pI 5.5) and C-ter (pI 9.9) peptides were conjugated to Affi-Gel-10 (Bio-rad) agarose beads for affinity-purification ...
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bioRxiv - Biochemistry 2020Quote: ... equilibrated with nanodisc buffer (100 mM KCl and 20 mM Tris/HCl pH 7.5) using an NGC Quest 10 chromatography system (BioRad). Concentration of ABCB10 in nanodiscs was estimated from Coomassie-stained gels using aliquots of known concentration of ABCB10 in detergent as reference ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear (5 µg total protein) and cytoplasmic (10 µg total protein) fractions were boiled in Laemmli buffer (Bio-Rad), run on Criterion 4-20% Gels (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... 10 μl of the DNA-coated microcarrier beads dried on 2.5 cm microcarrier discs (1652335 Bio-Rad, Hercules, CA). The manufacturer’s protocol for operation of the PDS-1000 system was followed ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were then separated by 10% Tricine–SDS–PAGE and then transferred to a polyvinylidene fluoride membrane (Bio-Rad) under the same conditions described above ...
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bioRxiv - Microbiology 2021Quote: ... Mab competent cells were incubated on ice with ~500 ng of DNA for 10 minutes and transformation was performed with the following settings (2.5kV, 25 uF, 1000ohms) using an electroporator (BioRad). Cells were recovered in 1ml of Middlebrook 7H9 broth incubated for 4 hrs at 37°C and inoculated onto Middlebrook 7H10 agar supplemented with appropriate antibiotics ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μg of protein was loaded and run on 10% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Separated proteins were transferred onto Nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Gels were flushed and loaded with 10 μg of total RNA in 2X TBE/Urea sample buffer (Bio-Rad), then run at 200 V until the bromophenol blue dye front reached the gel bottom ...
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bioRxiv - Cell Biology 2021Quote: ... the lysate that contains 10 μg of protein per sample was mixed with 2x Laemmli Sample Buffer (Bio-Rad) supplemented with 2 M 2-Mercaptoethanol (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... 8 mL column volume (CV)] with a quaternary amine strong anion exchanger (BIA Separations d.o.o, Ajdovščina, Slovenia) using a Bio-Rad BioLogic DuoFlow™ 10 chromatography system (Bio-Rad) set to 5 mL/min flow rate ...
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bioRxiv - Synthetic Biology 2022Quote: tRNA was analyzed by Urea-PAGE (Supplementary Fig. 2d) using a 10% Mini-PROTEAN® TBE-Urea Gel (BioRad). The gels were run at 120 V for 30 min then stained with SYBR-Safe gel stain (Thermo-Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... Bioscale Nuvia-IMAC Ni-charged and ENrich SEC70 and SEC650 10/300 size exclusion columns were purchased from Biorad, USA and used with BioRad NGC Quest plus FPLC system ...
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bioRxiv - Biophysics 2022Quote: ... 10 µL of 12x and 120x diluted protein prep was then loaded into a 15% SDS-PAGE gel (BioRad) with BioRad Precision Plus unstained standard for comparison ...
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bioRxiv - Cell Biology 2022Quote: ... then boiled in Laemmli buffer for 10 min and resolved on a precast 4 % - 15 % polyacrylamide gel (Bio-Rad). Proteins were transferred to nitrocellulose using a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Biophysics 2022Quote: ... Excess dye was removed via size exclusion chromatography using an ENrich™ SEC 650 10 x 300 Column (BioRad).
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bioRxiv - Neuroscience 2021Quote: ... cDNA was prepared from 10 to 50 ng RNA according to instructions of iScript™ cDNA Synthesis Kit (BioRad). Qiagen RNeasy® Plus mini kits (Qiagen ...
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Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionbioRxiv - Neuroscience 2021Quote: ... 12.1 ug of protein from each sample was loaded onto a 10% Tris-Glycine gel (Biorad, cat no. 4561034) and then transferred onto a PVDF membrane and blocked for one hour with 5% BSA in 0.1% Tween/1xTBS ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was made with 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels 10-well (BioRad, 4561094) with 30 μg of protein lysate for well and running was 70 V for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... and 30μg of total protein per sample was mixed with Laemmli buffer supplemented with 2-mercaptoethanol (10%) (Bio-rad) and boiled at 99°C for 10 minutes ...
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bioRxiv - Biophysics 2019Quote: ... followed by gel filtration in 10 mM sodium phosphate buffer (pH 7.4) using an ENrich SEC 650 size-exclusion chromatography column (BioRad). Purified samples were concentrated using Amicon Ultracel-3K centrifugal filter units (EMD Millipore).
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bioRxiv - Molecular Biology 2019Quote: ... The purity of the viral preparations was determined by SDS-PAGE analysis on a 10% stain free gel (Biorad) in Tris-Glycine-SDS buffer ...
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bioRxiv - Genetics 2019Quote: ... 10 mM L-serine) to a UapA concentration of 20 μM using Micro Bio-Spin 6 columns (Bio-Rad). UapA was loaded into gold coated capillaries and the protein sprayed into a Synapt G2-Si (Waters ...
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bioRxiv - Biochemistry 2019Quote: SEC separation was performed on a chromatographic column packed with P-10 media (Bio-Rad®, 45-90 μm) and collected by a Frac-920 fraction collector (GE Healthcare Life Sciences ...