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Citations for Bio-Rad :
1901 - 1950 of 8916 citations for 7 8 DIHYDRO 1 3 DIOXOLO 4 5 G ISOQUINOLIN 5 6H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... and placed into a precooled (5 °C) real-time PCR instrument (C1000™ Thermal Cycler and CFX96™ Real-Time System; Bio-Rad). For the fluorescence detection ...
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bioRxiv - Plant Biology 2021Quote: ... from Eurofins Genomics (https://www.eurofinsgenomics.eu/) were mixed and melted at 95◻°C for 5◻min in TE buffer in a T100™ thermocycler (Bio-rad). Then the denatured oligonucleotides were allowed to anneal as the temperature was reduced by 1 °C every 20 sec ...
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bioRxiv - Immunology 2020Quote: ... Reactions were performed in triplicates in a final reaction volume of 10 μl containing 5 μl of iQ™ SYBR® Green Supermix (Biorad, France), 4 μl of cDNA diluted 1:15 in DEPC-water and 0.5 μl of each forward and reverse primers at a final concentration of 0.5 μM (Table S2 ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 200 μl of 1X PBS per well and developed with 100 μl of TMB per well (Bio-Rad, #R8/R9) for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: A total of 5 μL of PCR amplicons were loaded onto a 1.5 % GelRed-stained agarose and the DNA bands were visualized using a gel documentation system (Gel Doc Ez Imager, Bio-Rad, CA, USA). PCR products were denatured at 95 °C for 10 min in the thermal cycler to avoid degradation by nucleases ...
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bioRxiv - Cancer Biology 2021Quote: ... NRF2 (CST, 12721S), KEAP1 (CST, 8047S), PRDX1 (CST, 8499S), GSTO1 (Thermo, PA5-83383)] in 5% BSA in TBS-T (Bio-Rad, 170-6435). Following incubation with primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: Cellular lysates were incubated at 95°C for 5 min and separated in 12% Mini-PROTEAN TGX Precast Protein gels (BIO-RAD, Cat #4561044). Proteins were then transferred to 0.45 μm PVDF membrane at 100V for 1 hr ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the 30°C incubation step was shortened to 5 hr using a Bio-Rad C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). DNA concentrations were measured by Qubit ...
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bioRxiv - Microbiology 2021Quote: ... the 30°C incubation step was shortened to 5 hr using a Bio-Rad C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). DNA concentrations were measured by Qubit ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of tGFP was monitored for 5 h at 30°C using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).
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bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by a final extension step at 68 °C for 5 min on the T100™ thermal cycler (Bio-Rad, Hercules, CA, USA). During protocol optimization ...
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bioRxiv - Microbiology 2022Quote: ... Expression and secretion samples were boiled for 5 min and then resolved on Mini-PROTEAN or Criterion™ TGX Stain-Free™ precast gels (Bio-Rad). For immunoblotting ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated with 50 µL/well of 5 µg/mL of mouse anti-bovine IFN-γ monoclonal antibody (mAb) (CC330, Bio-Rad Antibodies) by overnight incubation at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Biochemistry 2022Quote: ... and 20 μL of the sample was mixed with 5 μL of a 5X Laemmli sample buffer containing 10% β-mercaptoethanol (Bio-Rad Laboratories). The sample was boiled for 5 min at 95 °C and loaded into a 12% polyacrylamide gel ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Developmental Biology 2024Quote: ... and the reactions were stopped by boiling them at 95°C for 5 minutes after adding Laemmli sample buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol (Gibco 21985023) ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]). After removing the blocking buffer and re-applying PAP pen ...
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bioRxiv - Molecular Biology 2024Quote: ... The gels’ stain-free dye was activated by a 5-min UV exposure and protein was transferred to Immun-Blot low-fluorescence PVDF membrane (Bio-Rad #162-0264) using Hoefer TE77X ...
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bioRxiv - Cell Biology 2024Quote: ... boiled for 5 min at 95° and resolved on 10 % or 7.5 % Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033, 4561036, 4561023, 4561026). The SDS-PAGE was performed at 100 V for 5 minutes and then 120 V for 1 hour 15 minutes using SDS running buffer (30 g Tris ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated at 4 °C for 30 min and heated at 37 °C for 5 min prior to analysis on precast “any KD” Mini-PROTEAN TGX (Bio-rad, Cat #4569033) polyacrylamide gels using 1x TBE as running buffer ...
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bioRxiv - Neuroscience 2023Quote: 1 µg BH RNAs and 5 µl bdEV RNAs were used to generate cDNA by iScript ™ Reverse Transcription Supermix for RT-qPCR (Bio-Rad 1708840). Expression levels of circRNAs were evaluated by qPCR using iTaq Universal SYBR ® Green Supermix (Bio-Rad 1725120) ...
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bioRxiv - Physiology 2023Quote: ... 50µL from this mixture was transferred to a new tube containing 950µL of PBS and stored at 5°C for subsequent the cell counting (using a TC20 Automated Cell Counter, Bio-Rad, Solna, Sweden) 10 to 30 min later (when the mitochondrial measurements had started) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were loaded onto Any kD™ Mini-PROTEAN® TGX™ gels and separated under reducing conditions for 5 minutes at 250 V (Biorad). Proteins were visualized by Coomassie Blue Staining (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... heated in water bath for 5 minutes at 99°C and analysed by SDS-PAGE using Any-kD Mini-PROTEAN TGX Stain-Free gels (Bio-Rad, California, USA) in a 2-minute run for sample clean-up purposes ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Biochemistry 2023Quote: Standard protocols were adopted for SDS-PAGE in 10% isocratic or 5%-15% gradient polyacrylamide mini-gels (Bio-Rad Laboratories S.r.l., Segrate, Italy), and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores ...
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bioRxiv - Plant Biology 2023Quote: ... reactions were prepared in a 10 µL final volume containing 5 µL of 2x SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad), 0.125 µL of each specific primer pair at 250 nM ...
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bioRxiv - Cell Biology 2023Quote: ... denaturated at 95 °C for 5 min and separated on Bis-Tris polyacrylamide gels using the Mini-PROTEAN® Tetra Handcast System (Biorad, cat.no. 1658029FC). For visualization of total protein load ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... 50 µg of protein was denatured (95°C for 5 minutes) using lysis and Laemmli sample buffer (161-0737, Bio-Rad, with DTT). Samples were separated on a 10 % gradient gel (Criterion TGX Precast Gel ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting supernatant was diluted with 4x Laemmli sample buffer and boiled for 5 minutes before loading onto Mini-Protean TGX gels (Bio-Rad, Hercules, USA). Subsequently ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cell aliquots were thawed on ice for 5 minutes before adding 100 ng of plasmid DNA and electroporated on Bio-rad Micropulser (Bio-Rad, Hercules, CA) using EC2 protocol (0.2 cm cuvette ...
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bioRxiv - Immunology 2021Quote: ... and the ChemiDoc equipment with the Quantity One software (Bio-Rad Laboratories). Primary antibodies were used at 1:500 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... and Chemi-Lumi One Ultra (Nacalai Tesque) (for mouse) or ChemiDoc (BioRad) (for zebrafish) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the signals were measured using ImageQuant software (Quality One; Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... and the signals were measured using ImageQuant software (Quality One; Bio-Rad).
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bioRxiv - Molecular Biology 2019Quote: ... One cell per well was sorted into 384-well plates (Biorad, HSP3801) using the BD FACSJazz cell sorter ...
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bioRxiv - Neuroscience 2020Quote: ... Densitometric quantification of candidate proteins was performed using Quantity One software (BioRad). Values were normalized to GAPDH and a control reference sample ...
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bioRxiv - Plant Biology 2020Quote: ... Immuno-reactive proteins were detected with Clarity One detection reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ System (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... One microgram of mRNA was used to synthesize cDNA using iScript (Biorad). Kif9 and B-actin cDNAs were amplified by GoTag Green Master Mix (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... Images were processed using Quantity One software (Bio-Rad, Hercules, CA, USA) or Image Studio Lite 5.2 (LI-COR Biotechonology ...
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bioRxiv - Cell Biology 2021Quote: ... and separated by one-dimensional SDS-PAGE electrophoresis (Bio-rad, Berkeley, USA).
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... Band intensities in acquired images were quantified using Quantity One software (BioRad).
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bioRxiv - Molecular Biology 2019Quote: ... Lanes were scanned in the Quantity One software package (Bio-Rad, 1709602), background lane intensity was subtracted ...
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bioRxiv - Microbiology 2019Quote: ... One gel was stained with Coomassie Blue G250 (Bio-Rad, Hercules, CA) for mass spectrometry analysis ...
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bioRxiv - Neuroscience 2019Quote: ... blots were quantified with Quantity One analysis software (Bio-Rad, Hercules, CA). The results were expressed as the ratio to β-actin immunoreactivity.
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bioRxiv - Microbiology 2019Quote: ... quenched with one-third volume of 4X Laemmli Sample Buffer (Bio-Rad), and boiled for 5 min at 95°C.
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bioRxiv - Microbiology 2020Quote: ... One-Step RT-ddPCR advanced kit for probes (Bio-Rad, CA, USA) was used in our experiments ...