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Citations for Bio-Rad :
1751 - 1800 of 8354 citations for 7 8 DIHYDRO 1 3 DIOXOLO 4 5 G ISOQUINOLIN 5 6H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Color Development was obtained using the chromogenic substrate 4-chloro-1-naphthol (4CN, Bio-Rad) and H2O2.
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bioRxiv - Biochemistry 2024Quote: ... 4-20% (BioRad), or 6% (Thermofisher ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of ssDNA were added to 95 μl of VSV-G-EVs in trehalose buffer and EVs then transferred to a 1 mm electroporation cuvette (BioRad) placed on ice ...
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bioRxiv - Microbiology 2021Quote: ... protein G-magnetic beads (Bio-Rad, 161-4023) were added and incubated at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μl of protein G magnetic beads (Biorad) were added to each sample and incubated with rotation at 4 °C for 3 h ...
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bioRxiv - Biochemistry 2022Quote: ... The binding to protein G magnetic beads (Biorad) was performed for 1 hour at 4 ºC (with previous washes with a RIPA buffer) ...
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bioRxiv - Microbiology 2020Quote: ... boiled and electrophoresed on 12% vol vol−1 and 4% vol vol−1 acrylamide SDS-PAGE gels (Bio-Rad Laboratories, Canada) on MiniProtean® camera (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were subsequently blocked with 4% BSA and 1% Triton X-100 in PBS and incubated with rat anti-CD68 (1:500; Bio-Rad) and mouse-anti-GFP conjugated to Alexa Fluor 488 (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 hours followed by 20 μM Nigericin for 3 hours (ICT9146, Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... The protein samples were mixed with LDS sample loading buffer at 4X concentration followed by addition of beta-mercaptoethanol (5%) (Bio-Rad, Hercules, CA). The resultant mixture was denatured at 95°C for 9 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-Time reactions were performed in triplicates using KAPA SYBR FAST qPCR Kit (2X) on QuantStudio 5 Real-time PCR system (Bio-Rad, CA, USA) following the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled at 100°C for 5 min prior to SDS-PAGE separation on 12% acrylamide Mini-protean TGX precast gels (Bio-Rad Laboratories, US) and Western blot analysis.
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bioRxiv - Microbiology 2020Quote: ... 2,5 or 5 μL of the eluate were analysed by RT-qPCR using CFX96 Real-Time Thermal Cycler (Bio-Rad, Hercules, California, USA) as described elsewhere [76] with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... was electroporated into cells at 5×106 cells/0.4 ml under conditions of 220V and 950 µF using a Gene Pulser™ (Bio-Rad, Hercules, CA) and plated on DMEM containing 10% FBS.
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bioRxiv - Cell Biology 2021Quote: ... cDNA was diluted 1:10 and 5 μl of the diluted cDNA was loaded in a total volume of 20 μl (SYBR Green Supermix (Bio-Rad 172-5124) and run on the Bio-RAD CFX Connect Real-Time System ...
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bioRxiv - Genomics 2022Quote: ... The new plate with droplets was sealed with a pierceable PCR foil at 180 °C for 5 seconds using PX1 PCR Plate Sealer (Bio-Rad, Cat. 1814000). The PCR reaction was then carried out using the C1000 Touch™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... were co-electroporated with 1.5 x 107 KH2 ES cells at 400 V and 125 μf using two pulses in a Gene PulserII (Bio-Rad, Hercules, CA). Recombination between FRT sites such that the entire plasmid is inserted within the PGK promoter and the ATG initiation codon upstream and in frame with the hygromycin resistance gene was used to select correctly targeted cells ...
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bioRxiv - Developmental Biology 2022Quote: ... and up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 172-5038). Quantitative real time-PCR was performed using Go-Taq qPCR Master Mix (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were denatured for 5 min at 95°C and separated by SDS-PAGE using precast 10% polyacrylamide 10-well (Bio-Rad, #456-1034) or 18-well (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μl of each sample was taken out separately for measuring protein concentration using Bradford protein assay reagent (BIO-RAD, Hercules, CA, USA). 5% of the lysates were boiled with 2 x laemmli buffer (BIO-RAD ...
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bioRxiv - Bioengineering 2020Quote: ... and placed into a precooled (5 °C) real-time PCR instrument (C1000™ Thermal Cycler and CFX96™ Real-Time System; Bio-Rad). For the fluorescence detection ...
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bioRxiv - Plant Biology 2021Quote: ... from Eurofins Genomics (https://www.eurofinsgenomics.eu/) were mixed and melted at 95◻°C for 5◻min in TE buffer in a T100™ thermocycler (Bio-rad). Then the denatured oligonucleotides were allowed to anneal as the temperature was reduced by 1 °C every 20 sec ...
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bioRxiv - Immunology 2020Quote: ... Reactions were performed in triplicates in a final reaction volume of 10 μl containing 5 μl of iQ™ SYBR® Green Supermix (Biorad, France), 4 μl of cDNA diluted 1:15 in DEPC-water and 0.5 μl of each forward and reverse primers at a final concentration of 0.5 μM (Table S2 ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 200 μl of 1X PBS per well and developed with 100 μl of TMB per well (Bio-Rad, #R8/R9) for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: A total of 5 μL of PCR amplicons were loaded onto a 1.5 % GelRed-stained agarose and the DNA bands were visualized using a gel documentation system (Gel Doc Ez Imager, Bio-Rad, CA, USA). PCR products were denatured at 95 °C for 10 min in the thermal cycler to avoid degradation by nucleases ...
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bioRxiv - Cancer Biology 2021Quote: ... NRF2 (CST, 12721S), KEAP1 (CST, 8047S), PRDX1 (CST, 8499S), GSTO1 (Thermo, PA5-83383)] in 5% BSA in TBS-T (Bio-Rad, 170-6435). Following incubation with primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: Cellular lysates were incubated at 95°C for 5 min and separated in 12% Mini-PROTEAN TGX Precast Protein gels (BIO-RAD, Cat #4561044). Proteins were then transferred to 0.45 μm PVDF membrane at 100V for 1 hr ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the 30°C incubation step was shortened to 5 hr using a Bio-Rad C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). DNA concentrations were measured by Qubit ...
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bioRxiv - Microbiology 2021Quote: ... the 30°C incubation step was shortened to 5 hr using a Bio-Rad C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). DNA concentrations were measured by Qubit ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of tGFP was monitored for 5 h at 30°C using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).
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bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by a final extension step at 68 °C for 5 min on the T100™ thermal cycler (Bio-Rad, Hercules, CA, USA). During protocol optimization ...
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bioRxiv - Microbiology 2022Quote: ... Expression and secretion samples were boiled for 5 min and then resolved on Mini-PROTEAN or Criterion™ TGX Stain-Free™ precast gels (Bio-Rad). For immunoblotting ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated with 50 µL/well of 5 µg/mL of mouse anti-bovine IFN-γ monoclonal antibody (mAb) (CC330, Bio-Rad Antibodies) by overnight incubation at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Biochemistry 2022Quote: ... and 20 μL of the sample was mixed with 5 μL of a 5X Laemmli sample buffer containing 10% β-mercaptoethanol (Bio-Rad Laboratories). The sample was boiled for 5 min at 95 °C and loaded into a 12% polyacrylamide gel ...
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bioRxiv - Neuroscience 2023Quote: 1 µg BH RNAs and 5 µl bdEV RNAs were used to generate cDNA by iScript ™ Reverse Transcription Supermix for RT-qPCR (Bio-Rad 1708840). Expression levels of circRNAs were evaluated by qPCR using iTaq Universal SYBR ® Green Supermix (Bio-Rad 1725120) ...
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bioRxiv - Physiology 2023Quote: ... 50µL from this mixture was transferred to a new tube containing 950µL of PBS and stored at 5°C for subsequent the cell counting (using a TC20 Automated Cell Counter, Bio-Rad, Solna, Sweden) 10 to 30 min later (when the mitochondrial measurements had started) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were loaded onto Any kD™ Mini-PROTEAN® TGX™ gels and separated under reducing conditions for 5 minutes at 250 V (Biorad). Proteins were visualized by Coomassie Blue Staining (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... heated in water bath for 5 minutes at 99°C and analysed by SDS-PAGE using Any-kD Mini-PROTEAN TGX Stain-Free gels (Bio-Rad, California, USA) in a 2-minute run for sample clean-up purposes ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Biochemistry 2023Quote: Standard protocols were adopted for SDS-PAGE in 10% isocratic or 5%-15% gradient polyacrylamide mini-gels (Bio-Rad Laboratories S.r.l., Segrate, Italy), and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores ...
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bioRxiv - Plant Biology 2023Quote: ... reactions were prepared in a 10 µL final volume containing 5 µL of 2x SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad), 0.125 µL of each specific primer pair at 250 nM ...
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bioRxiv - Cell Biology 2023Quote: ... denaturated at 95 °C for 5 min and separated on Bis-Tris polyacrylamide gels using the Mini-PROTEAN® Tetra Handcast System (Biorad, cat.no. 1658029FC). For visualization of total protein load ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... 50 µg of protein was denatured (95°C for 5 minutes) using lysis and Laemmli sample buffer (161-0737, Bio-Rad, with DTT). Samples were separated on a 10 % gradient gel (Criterion TGX Precast Gel ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Developmental Biology 2024Quote: ... and the reactions were stopped by boiling them at 95°C for 5 minutes after adding Laemmli sample buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol (Gibco 21985023) ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]). After removing the blocking buffer and re-applying PAP pen ...