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Citations for Bio-Rad :
1851 - 1900 of 2599 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were eluted by heating the beads (95°C for 5 min.) in 70 μL of BME 4X Laemmli buffer (Biorad #1610747). Prior to loading on a 7.5% TGX SDS-PAGE gel (Biorad #5671024) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a 10 min final extension at 72°C before resolving on a 5% polyacrylamide tris-borate-EDTA (TBE) gel (Bio-Rad) at 35 V ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were washed three times in TBST for 5 minutes each and incubated with an anti-mouse HRP secondary (Bio-Rad) at 1:2000 dilution for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 20µg of protein samples were heated in 1X Laemmli buffer at 95°C for 5 min and loaded on pre-cast 4-20% SDS-polyacrylamide electrophoresis gel (Bio-Rad). For the RIP experiment ...
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bioRxiv - Immunology 2024Quote: ... cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad, Hercules, CA) and 5 μM each of gene-specific forward and reverse primers ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of synthesized cDNA was firstly diluted in a 1:5 ratio and used in a 20 µL reaction volume using the CFX96 Touch Real-Time PCR Detection System (BioRad, USA) with the following thermal cycling conditions ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Cell Biology 2023Quote: ... 10µg and 5 µg /sample) were resolved by SDS-PAGE on Mini-PROTEAN TGX Stain-Free Gels (Cat. # 4568086; Bio-Rad) and were run alongside a Page Ruler prestained protein ladder (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... 2 × 106 of MEL cells were suspended in 100ul of High-Performance Electroporation Solution (BTXpress, 45-0801) with 5-10 µg of plasmid DNA and electroporated with the Gene Pulser Xcell System (Bio-Rad) using 2 pulses at 200 V for 5 ms ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extractions containing 5 µg of Chl with 1 x SDS loading buffer were boiled at 100℃ for 5 min and loaded onto a 4 - 20% polyacrylamide gel (Mini Protean TGX, Biorad Laboratories). Proteins were transferred to a PVDF-FL membrane on a Biorad semidry blotting system ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µl of input and 5 µl of IP samples were loaded and protein samples were separated on 4-20% Tris-glycine polyacrylamide gels (Bio-Rad) at 170 V for 1 hour in 1X Tris/glycine/SDS running buffer ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was washed three times with T-TBS and twice with TBS for 5 min each and incubated with Clarity Western ECL substrate (Bio-Rad). After 5 min ...
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bioRxiv - Microbiology 2023Quote: Total protein extracts (5×106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
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bioRxiv - Microbiology 2023Quote: A volume of 5 μL of cDNA was used for quantitative PCR (qPCR) on a CFX Connect Real- Time PCR Detection System (Bio-Rad). TaqMan and SYBR Green qPCRs were performed as described previously (Benschop et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCR) was performed in a 10 µL reaction that contained 5 µL SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µL H2O ...
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bioRxiv - Biophysics 2023Quote: ... samples were processed using a Bio-Rad NGC Quest 10 Plus fast protein liquid chromatography (FPLC) apparatus with a 5 mL EconoFit Nuvia IMAC affinity column (Bio-Rad). To purify protein from the soluble fraction ...
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bioRxiv - Cell Biology 2023Quote: ... 3 μl ten-fold diluted cDNA preparation was mixed in a final volume of 10 μl containing 5 μl iQ SYBR Green Supermix (Bio-Rad) and 4 pmol of each primer (Fw ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein was denatured at 95°C for 5 min in 1x Laemmli buffer containing 5% β-mercaptoethanol and separated in 4-15% mini-PROTEAN TGX Precast Protein Gels (Biorad) by electrophoresis and blotted onto Nitrocellulose membrane (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then boiled at 95°C for 5 minutes and 20 μL of the samples were loaded into a pre-made polyacrylamide gel (Bio-Rad) immersed in 1x Tris-Glycine-SDS Buffer ...
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bioRxiv - Microbiology 2023Quote: ... Samples (24 µg) were denatured by heating 5 minutes at 95°C and separated by Stain-Free SDS-PAGE gel (4568096, Bio-Rad). The gel was activated using one-minute setting (Bio-Rad Imager ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured by 5 min incubation at 95°C before running them on a TGX 4–12% or 10% gel (Bio-Rad) and transferred onto nitrocellulose membrane for hybridization with specific antibodies.
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed 3 times with TBST for 5 min each and then the membrane was incubated with Clarity Western ECL Substrate (Bio-Rad) for 2 min before exposure to film and development in a darkroom.
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bioRxiv - Cancer Biology 2023Quote: ... Nitrocellulose membranes were blocked for 1h at RT in 5% Blotting Grade Blocker Non-Fat Dry Milk (Biorad, Hercules, CA, USA) and TBST ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fractions containing Chimera were pooled and further purified by ion exchange chromatography (IEX) using a 5 ml Bio-Scale Mini Macro Prep High Q (Bio-Rad) equilibrated in Buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins samples were denatured in SDS-sample buffer for 5 min at 95°C and loaded onto Mini-Protean TGXTM gels (BioRad Laboratories) in equal amounts and subjected to electrophoresis ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... The membrane was incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies in 5% milk and bands were developed with Chemi-Doc XRS imaging (Bio-Rad). The primary antibodies used are listed in Supplementary Table 3.
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bioRxiv - Cancer Biology 2022Quote: ... Samples were boiled at 95 °C for 5 minutes and loaded to SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to nitrocellulose membranes (162-0112, Bio-Rad). Immunoblot analysis was performed with the following antibodies ...
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bioRxiv - Biophysics 2022Quote: ... Digestion was confirmed by running 5 µL of digested or diluted samples on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad), followed by staining with Gelcode Blue (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Zero-minute timepoints were collected by independently adding 5 µL of each mixture to 10 µL 2X Laemmli Buffer (Bio-Rad) and boiling at 95 °C for 5 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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bioRxiv - Cell Biology 2022Quote: ... on ice for 24–36 h and then removed through glutathione sepharose 4B beads (5 mL bed volume) packed twice in Poly-Prep® Chromatography Columns (BioRad). UtrCH-sfGFP and LifeAct-sfGFP were further purified through gel filtration column chromatography (HiLoad 16/600 Superdex 75 pg ...
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Immunology 2022Quote: ... were amplified by PCR using 5’ and 3’ primers with overhangs containing T7 binding sites using iProof High-Fidelity Taq (Bio-Rad). PCR reaction products were run on an agarose gel to check for the correct amplicon ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg protein per sample was boiled for 5 min in sample loading buffer and separated by SDS-PAGE using the Mini-PROTEAN electrophoresis system (Bio-Rad). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked by incubating in 15 mL of blocking solution (5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Samples were boiled for 5 min at 95°C before separation on a gradient 4 to 20% sodium dodecyl sulfate (SDS)-polyacrylamide gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semidry transfer (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:5 in deionized water and used as the template for quantitative PCR with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). For relative quantification ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Neuroscience 2022Quote: ... at either 95°C for 5 minutes or at 70°C for 10 minutes and resolved in a 4-15% Precast Gels (Bio-Rad, 12-well ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ // Reverse: 5’-CGA AGG TGT GAC TTC CAT G-3’) and CFX96 Touch Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred onto Immun-Blot PVDF membrane (BioRab, 1620177) and incubated overnight in blocking solution (5% powdered milk, NaF in TBST [TBS (BioRad, 1706435) 1% Tween] ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing ADDoCoV were pooled and further purified by ion exchange chromatography (IEX) using a 5 ml Bio-Scale Mini Macro Prep High Q (Bio-Rad) equilibrated in Buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: NKX2-5 homeodomain purity was evaluated through SDS-PAGE using 15 well Mini-PROTEAN TGX Precast Protein Gels® (Bio-Rad). Samples were prepared with 4X loading buffer containing β-mercaptoethanol (BME ...