Labshake search
Citations for Bio-Rad :
1701 - 1750 of 2599 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... Both PK-treated and untreated samples were then mixed 1:2 with 4X Laemmli sample buffer (Bio-Rad) containing DTT ...
-
bioRxiv - Developmental Biology 2020Quote: ... Smit1/2 and TonEBP were measured by real time qPCR using SYBR Green I (Bio-Rad, Mississauga ON) and 5 μM of forward and reverse primer mix (Bio-Rad CFX384 ...
-
bioRxiv - Genetics 2021Quote: ... The enriched proteins were dissociated by the addition of 2 x Laemmli sample buffer (161-0737, Bio-Rad) and boiled at 95 °C for 10 min ...
-
bioRxiv - Biochemistry 2021Quote: KirBac3.1 protein was buffer-exchanged to 200 mM ammonium acetate (pH=8.0) with 0.5% C8E4 (2×CMC) using a Biospin-6 column (BioRad). The protein sample was loaded into a gold coated needle prepared in-house and analysed on a modified Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2021Quote: ... Gels were dried for 2 h at 80°C and analysed on a Personal Molecular Imager (Bio-Rad). For RNA stability assays ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μl of RNA were mixed with 2 μl of 50 μM oligo dT primer (Bio-Rad, 1725038) and 1 μl of 25 mM dNTP mix (NEB ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-α-tubulin YL1/2 from rat (MCA77G, Bio-Rad AbD Serotec, at 1:2000 dilution for IF), anti-rabbit Alexa Fluor 488 (A11008 ...
-
bioRxiv - Immunology 2022Quote: ... 200 μL of this transformation mix was then aliquoted into pre-chilled 2 mm electroporation cuvettes (Bio-Rad) and electroporated at 1500 V with an average time constant of ∼4.5 ms using a Gene Pulser Xcell Electroporation System (Bio-Rad) ...
-
bioRxiv - Physiology 2023Quote: ... Protein samples were suspended in Laemmli Sample Buffer supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Then ...
-
bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed using the Promega 2-step kit on a CFX96 Real-Time PCR System (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
-
bioRxiv - Plant Biology 2023Quote: ... 2 ug RNA was used to synthesize cDNA with the iScript™ cDNA Synthesis Kit (Bio-Rad 1708890). The SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad 1725271 ...
-
bioRxiv - Physiology 2023Quote: ... and samples of four larvae each were homogenized in 100 μl 2× SDS sample buffer (Bio-Rad #1610737) containing 5% (355 mM ...
-
bioRxiv - Cell Biology 2023Quote: ... Three hundred micrograms of protein was precipitated using a ReadyPrep 2-D Cleanup Kit (Bio-Rad Laboratories, USA) at −20 °C overnight ...
-
bioRxiv - Immunology 2023Quote: ... unreacted ITC-DTPA was removed by dialysis against 0.25 M ammonium acetate (NH4Ac) pH 5.4–5.5 (metal free) supplemented with 2 g/L Chelex (Bio-Rad) using 20,000 molecular weight cut-off dialysis cassettes (Slide-a-Lyzer ...
-
bioRxiv - Cancer Biology 2024Quote: ... 2 μg of total RNA was used for cDNA preparation using the iScript cDNA Synthesis Kit (Bio-Rad). A 1/10 dilution of the cDNA was then used for qRT-PCR analysis utilizing the SYBR Green PCR Master Mix (Applied Biosystems) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The mixture was transferred to an ice-cold 2 mm gap electroporation cuvette (Bio-Rad Laboratories GmbH, Germany), and cells were electroporated with a single pulse at 1.8 kV ...
-
bioRxiv - Genomics 2023Quote: ... Gene panel probe hybridization occurred overnight for 18 hours at 50°C (Bio-Rad DNA Engine Tetrad 2). Subsequent washes the next day removed unbound probes ...
-
bioRxiv - Cell Biology 2023Quote: Protein samples were added with equal volume of 2× Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA) containing 65.8 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein was then cleaned 2-times on Micro Bio-Spin™ P-6 gel Columns (BioRad, #732-6221) where buffer was exchanged with phase separation buffer ...
-
bioRxiv - Genomics 2023Quote: ... proteins were transferred (80 V; 2 h; 4°C) onto a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Membranes were air-dried ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was passed twice on 2 x Mini CHT ™ type I column (Bio-Rad, United States). Elution was performed with a gradient of 0 to 200 mM NaPO42- in 10 min ...
-
bioRxiv - Plant Biology 2022Quote: ... For α-ACTIN-2 the secondary antibody Goat Anti-Mouse IgG (H + L)-HRP conjugate (1706516, Bio-rad) was used at a dilution of 1:5000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Captured proteins were separated from beads by incubating beads in 50 μl 2× Laemmli Sample Buffer (Bio-Rad) at 95°C for 25 min ...
-
bioRxiv - Biochemistry 2023Quote: ... The detergent was removed by three 2-hour incubations with ∼33 mg of Bio-Beads SM2 (Bio-Rad) followed by overnight incubation with ∼50 mg of Bio-Beads SM2 with all incubations occurring at 4°C.
-
bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from total RNA (1-2 μg) with iScript Advanced cDNA Synthesis Kit (Bio-Rad; 1725038). Quantitative RT-PCR (qPCR ...
-
bioRxiv - Neuroscience 2023Quote: ... cDNA synthesis was performed using 2 μg of total RNA and the iScript cRNA synthesis kit (Bio-Rad). qPCR was performed using the Brilliant III ultra-Fast SYBR green qPCR master mix (Agilent Technologies ...
-
bioRxiv - Biochemistry 2024Quote: ... at 45 V for 2 h using the Mini-PROTEAN Tetra Vertical Electrophoresis Cell System (Bio-Rad, 1658029FC) and the blotting buffer containing 0.1 M glycine (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: Thirty micrograms of protein were denatured in 2× Laemmli sample buffer (Cat. No. 1610737, Bio-Rad, Hercules, CA) containing 4% β-mercaptoethanol at 100 °C for 5 min before loading into 4-20% SDS-polyacrylamide gels ...
-
bioRxiv - Microbiology 2024Quote: ... and 2 µL of each reaction was mixed with 10 µL of 1x Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 µL of cDNA was used per qPCR reaction with iQ SYBR Green Supermix (Bio-Rad, Hercules, CA) and 300 nM of each sense and antisense primer (20 µL total reaction volume) ...
-
bioRxiv - Biophysics 2024Quote: ... The hC1q sample was mixed at a 1:2 ratio with 2x Laemeli Sample Buffer from Bio-Rad. For the reducing conditions the Laemeli sample buffer also contained 2-β-mercaptoethanol ...
-
bioRxiv - Biochemistry 2024Quote: ... detergent was removed by addition of 0.8 grams ml-1 reaction buffer of Bio-Beads SM-2 (Biorad) prewashed in storage buffer and incubation on a roller for 2 hours at room temperature (RT) ...
-
bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Real time quantitative PCR (qRT-PCR) was performed using an iQ™5 Multicolor Real-Time PCR Detection System (BIO-RAD) with qPCRBIO SyGreen Mix fluorescein (PCR BIOSYSTEMS) ...
-
bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
-
bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... The blots were washed four times with TBST for 5 minutes each before developing with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging ...
-
bioRxiv - Immunology 2021Quote: ... They were incubated at the room temperature for 5-10 min and they were placed inside a 4-mm cuvette (Bio-Rad). The cuvettes were pulsed using the BTX electroporator (300 V ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were washed three times with TBST for 5 minutes and incubated with Goat Anti-Rabbit IgG (Bio-Rad, 170-6515) for 1 hour at RT ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...
-
bioRxiv - Developmental Biology 2020Quote: ... 50 seconds at 72°C and a final extension of 5 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). In order to standardize the expression pattern of NvDsx ...
-
bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...
-
bioRxiv - Cell Biology 2022Quote: ... Up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega ...
-
bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
-
bioRxiv - Developmental Biology 2020Quote: ... excess antibody subsequently removed by a further 4 washes in TBSTw for 5 minutes before detection with ECL Western blotting reagent (Bio-Rad) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were washed 3 times for 5 min with TBST after secondary antibody incubation and chemiluminescence signal was detected using Clarity Western ECL Substrate (Bio-Rad). All relevant information on antibodies used in this study are listed in Table S2.
-
bioRxiv - Bioengineering 2021Quote: ... This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad) for 1 h with rocking ...
-
bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...