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Citations for Bio-Rad :
1801 - 1850 of 7728 citations for Estrone 3 Glucuronide E1G ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and mixed 1:1 with 2X Sample Loading Buffer (Bio-Rad) + 5% β-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 0.1% Tween-20 in 1x PBS (PBS-T ...
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bioRxiv - Bioengineering 2021Quote: ... and probed overnight at 4°C with IRDye 680 Streptavidin (LICOR) diluted 1:3000 in 1:1 TBST:Blocking Buffer (BioRad). Membranes were imaged using an Azure Biosystems c600 ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated with the primary antibodies (rabbit anti-cPLA2, 1:100, Cell Signalling; mouse anti-LaminA/C, 1:500, ABCAM; rat anti-tyrosinated α-tubulin 1:500, Bio-Rad; mouse γ-H2AX, 1:2000) in blocking solution for 1h at room temperature (RT) ...
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bioRxiv - Biophysics 2021Quote: ... and transfected with Fluorescence Multiplex-Host Cell Reactivation (FM-HCR) reporter plasmids using the Gene Pulser MXCell Plate Electroporation System (Bio-Rad, #165-2670) using conditions 260 V ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed on 96-well optical reaction plates with one-step SYBR Green PCR master mix (Bio-Rad Laboratories, Cat#1725150).
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bioRxiv - Microbiology 2021Quote: ... The bound dye in the wells was eluted with 33% acetic acid and the absorbance of the solution was read at 595nm in a microtitre plate reader (BioRad i-Mark, Japan). To assess the individual bacterial population during growth ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated for 24 h in an incubator and the optical density was read at 595nm in a microtitre plate reader (BioRad i-Mark, Japan). Growth inhibition percentage was calculated by using the formula:
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bioRxiv - Cancer Biology 2020Quote: ... Wells were filled with E3 containing 0.28ug/mL Tricaine-S and the plate sealed with a PCR microseal (BioRad Microseal ‘B’ Film, #MSB1001). Up to 45 larval zebrafish per experiment were imaged in parallel on a GE IN Cell Analyzer 6000 every 15-20 minutes for 24-30h ...
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bioRxiv - Bioengineering 2021Quote: ... The obtained DNA extract (100 μL) was stored at −20 °C until further investigation and subjected to qPCR analysis using 96-well plates (Bio-Rad, Hercules, USA), the Thermocycler CFX connect system (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: ... The new plate with droplets was sealed with a pierceable PCR foil at 180 °C for 5 seconds using PX1 PCR Plate Sealer (Bio-Rad, Cat. 1814000). The PCR reaction was then carried out using the C1000 Touch™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad, Hercules, CA). The result is the representative from one individual’s CD14+ samples and a total of 2 healthy individuals’ CD14+ monocytes were tested ...
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bioRxiv - Microbiology 2022Quote: ... Amplification was carried out in 96-well plates using a CFX-96 cycler equipped with CFX Maestro software (Bio-Rad, Hercules, CA, USA). The thermocycling conditions were as follows ...
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bioRxiv - Genomics 2019Quote: ... We carried out real time PCR reaction and analyses in 384-well optical reaction plates using the CFX384 instrument (Bio-Rad, Hercules, CA).
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bioRxiv - Pathology 2020Quote: ... Real-time RT-PCRs were carried out in 96-well plates on a Bio-Rad CFX96™ Real-Time PCR system (Bio-Rad Laboratories,) or a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... We then prepared eight four-fold serial dilutions of this cytokine/chemokine protein standard pool and transferred 50 µL of each dilution to a 96-well Bio-Plex Pro™ Flat bottom plate (BioRad, Catalog# 171025001). Final standard curves were optimized and generated using Bio-Plex Manager 6.1 software ...
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bioRxiv - Microbiology 2021Quote: ... 12 – 16 individual colonies from each condition were selected from the m-TSAYE plates and DNA was extracted using Bio-Rad InstaGeneTM matrix (Bio-Rad, Hercules, CA) following the manufacturer’s protocol for DNA extraction from bacterial cells ...
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bioRxiv - Immunology 2021Quote: ... according to the instructions provided by the manufacturer and the plate were then read using a Luminex-based Bio-plex Multiplex system (Bio-Rad, Hercules, CA). Samples were diluted to 1:3 for PEG− 2 and LTB4 analysis and 1:20 for IL-8 measurements.
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bioRxiv - Microbiology 2021Quote: All melt-MAMA PCRs were performed on a CFX96 Real-Time PCR Detection System using white hard shell 96-well PCR plates or 0.2 mL 8-tube PCR strips (Bio-Rad, Gladesville, NSW, Australia), and PCR results examined using the CFX Maestro v4.1.2433.1219 software (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... 100µl of 1xPBS was added to each well and the plate was covered with a foil seal (catalog# MSF-1001, Bio-Rad, Hercules, CA). Images were taken on an InCell 6000(GE Healthcare Bio-Sciences ...
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bioRxiv - Synthetic Biology 2022Quote: ... the plates were analyzed for luminescence signals using a ChemiDoc Imaging System and Image Lab 6.0.1 software (Bio-Rad Laboratories Inc., Hercules, USA). Exposure time was set to 5 sec ...
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bioRxiv - Bioengineering 2023Quote: ... Amplification was carried out in 96-well plates using a CFX-96 cycler equipped with CFX Maestro software (Bio-Rad, Hercules, CA, USA). The thermocycling conditions were as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... and cDNA template were loaded onto a 384-well plate and run through 40 cycles on a CFX384 real time cycler (Bio-Rad Laboratories, Inc). The data were analyzed using the manufacturer’s CFX manager software ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and cDNA template were loaded onto a 384-well plate and run through 40 cycles on a CFX384 real time cycler (Bio-Rad Laboratories, Inc). The data were analyzed using the manufacturer’s CFX manager software ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification reactions were conducted in 96-well optical plates on a Bio-Rad CFX Connect using 2x iTaq Universal SYBR Green Supermix (Bio-Rad, California, USA), 0.25 µM of each primer ...
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bioRxiv - Developmental Biology 2023Quote: ... The assay was performed in a 96-well plate in a final volume of 200 µL containing the sample and Bradford’s solution (BioRad, Marnes-la-Coquette, France). After a 5-min incubation at RT ...
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bioRxiv - Cancer Biology 2024Quote: Total protein concentrations were determined against a standard curve of BSA in a 96-well plate using the DC™ Protein assay (Bio-Rad Laboratories). Proteins were separated on reducing 10% NuPAGE™ Novex™ Bis-Tris Protein Gels and transferred onto PVDF membranes ...
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bioRxiv - Biochemistry 2020Quote: To visualize protein expression 1 μL of the TXTL reactions was mixed with 2 μL of water and 3 μL of 2x Laemmli loading dye (Bio-Rad, Hercules, CA, USA) and incubated at 90°C for 3 min ...
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bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: Frozen muscle biopsies were homogenised in modified Laemmli sample buffer as described previously.37 Samples were run in Bio-Rad Criterion 3–8 % tris-acetate gradient gels (Bio-Rad Laboratories, CA, USA) at room temperature ...
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bioRxiv - Genetics 2022Quote: ... the membranes were washed 3 times for 10 min each with TBST buffer at 24 °C and visualized using enhanced chemiluminescence reagents (Bio-Rad Laboratories, CA, USA). The images were captured using Image Quant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... and O’nyong yong virus (ONNV) were subjected to a thermal gradient treatment from 30 to 60 °C for 3 h with a thermocycler (Bio-Rad T100 Thermal Cycler), after which samples were immediately titrated on Vero cells ...
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bioRxiv - Microbiology 2022Quote: ... the indicated ESA supernatant was boiled in SDS-PAGE sample buffer and 3 μg of each sample was run with a 4-15% TGX gradient gel (Bio-Rad cat. no. 4561086), then transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... and the miR200c primers (upstream primer, 5’- TAATACTGCCGGGTAATGATGGA-3’) (Eurofins Genomics) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). U6 primers (TAKARA ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined by the Bradford method using a Bio-Rad protein assay kit or Lowry’s assay with a DC protein assay kit (Bio-Rad Laboratories). TonB2 was detected using the ECL Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... RNA was extracted with the Nucleospin RNA Isolation Kit (Machery-Nagel) and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). TaqMan real-time qPCR assays were used for quantification of human IFNB1 (Hs01077958_s1) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted using the PicoPure RNA Isolation kit and cDNA synthesis was performed using the iScript cDNA synthesis kit (Bio-Rad). cDNA samples were amplified with SYBR Select mastermix (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were determined by the Bradford method using a Bio-Rad protein assay kit or Lowry’s assay with a DC protein assay kit (Bio-Rad Laboratories). TonB1 and DdvT were detected using the ECL Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA for PCR analyses was made with a commercially available kit (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). The quantification of mRNA expression was performed using the SYBR Green dye (Bio-Rad SsoAdvanced™ - Universal SYBR® Green Supermix ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated either from homogenized mosquitoes or infected cell supernatant using a Multisource RNA Miniprep Kit (AP-MN-MS-RNA-250, Axygen) and reverse transcribed to cDNAs using an iScript cDNA Synthesis Kit (1708890, Bio-Rad). Viral genomes were quantified by qPCR using iTaq Universal SYBR Green Supermix (1725121 ...
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bioRxiv - Microbiology 2021Quote: ... using the primer-probe mix from the 2019-nCoV RUO kit (IDT) with iTaq Universal Probes One-Step kit (Bio-Rad). The 2019-nCoV_N_Positive Control plasmid (IDT ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted using the RNeasy plus micro RNA extraction kit followed by reverse transcription using iScript cDNA synthesis Kit (Bio-Rad). Equal amounts of DNA and corresponding primers (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using the NucleoSpin RNA Plus Kit (Machery-Nagel) followed by reverse-transcription with the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1μg of total RNA was reverse transcribed into cDNA using a high-capacity cDNA reverse transcription kit (iScript cDNA Synthesis Kit, Bio-Rad) according to the provided protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with horseradish peroxidase conjugated secondary antibodies (1:5,000 or 1:10,000 in non-fat dry milk, 1 h, room temperature; Bio-Rad Laboratories). Secondary antibodies were visualized using enhanced chemiluminescence (ECL Plus ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was washed three times with T-TBS for 3 x 5 min and incubated for 1 h with 1/1000 solution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (1:1000; Biorad) and washed 3 times with T-TBS and 1 time with TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore; Goat anti-rabbit HRP 1:3000, BioRad)
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bioRxiv - Molecular Biology 2021Quote: ... 1:3000 or 1:10000 (for H3) dilutions of secondary antibodies (BioRad) (1 h at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... according to 1:1 by adding appropriate amount of ECL (Bio-Rad) color solution in the dark conditions and photographed using an EZ-ECL chemiluminometer Detection Kit for HRP (Biological Industries ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was diluted 1:1 with 2X Laemmli sample buffer (BioRad, 1610737) under reducing conditions and boiled for 5 minutes ...