Labshake search
Citations for Bio-Rad :
1651 - 1700 of 7728 citations for Estrone 3 Glucuronide E1G ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... 6 ng of cDNA was used per qPCR reaction consisting of gene-specific primers (Supplementary Information Table 3) and amplified with the PowerSYBR Green Master Mix (Applied Biosystems in a CFX-96 thermocycler (Bio-Rad). Data was acquired using the Bio-Rad CFX Manager 3.1 ...
-
bioRxiv - Neuroscience 2019Quote: ... 1 ml Tween20 add to 1000 ml with ddH2O) 3 times for 20 min and blocked with 10% dried milk-TBST solution or BlockAce-TBST solution (BlockAce, Bio-Rad, #170223) for 2 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... Band intensities of at least 3 individual experiments were measured densitometrically and normalized to whole protein using the Image Lab Software 5.1 from BioRad (Biorad, Munich, Germany).
-
bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
-
bioRxiv - Neuroscience 2022Quote: ... All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). Relative expression levels were determined according to the ΔΔCt method with the human housekeeping gene ABELSON as endogenous control for normalization as previously described (Beillard et al. ...
-
bioRxiv - Developmental Biology 2019Quote: Oocytes were collected in 0.1% PVP in DPBS and boiled for 3 mins at 95°C in 1x Laemmli Sample Buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol ...
-
bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
-
bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using 3 ng (neutrophils) and 10 ng (monocytes) of total RNA and iScript™ Reverse Transcription Supermix (Bio-Rad). Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad) ...
-
bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
-
bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were heated to 50°C for 2 to 3 min before the addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexed vigorously for 5 sec before pipetting in plug mould (Bio-Rad 1703713) ...
-
bioRxiv - Systems Biology 2023Quote: ... was applied for 3 min before the membranes were imaged using the ChemiDocXRS+imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Quantification of proteins was conducted using Image J software (Version 1.52a ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
-
bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
-
bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
-
bioRxiv - Microbiology 2021Quote: ... using the primer-probe mix from the 2019-nCoV RUO kit (IDT) with iTaq Universal Probes kit (Bio-Rad). Quantitation of actin transcripts was performed by a SYBR green assay ...
-
bioRxiv - Cancer Biology 2019Quote: ... Reverse transcription iScript cDNA synthesis kit and SSo Advanced Universal SYBR Green kit were from Bio-Rad (Solna, Sweden)25.
-
bioRxiv - Physiology 2021Quote: ... and mixed 1:1 with 2x Laemmli buffer (Bio-Rad) plus 5% β-mercaptoethanol ...
-
bioRxiv - Pathology 2019Quote: ... F4/80 (Clone Cl:A3-1, Bio-Rad, MCA497R, 1:100), CD3 (Abcam ...
-
bioRxiv - Microbiology 2021Quote: ... The blot was stained with 1:1 enhancer:substrate reagents (BioRad) for 5 min ...
-
bioRxiv - Neuroscience 2021Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
-
bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
-
bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein was denatured with 1:1 Laemlli loading buffer (BIORAD) by boiling for 10 minutes on a dry bath (Thermolyne Dri-Bath ...
-
bioRxiv - Biochemistry 2023Quote: ... The collected cells were mixed 1:1 with TrypanBlue (BioRad) and counted.
-
bioRxiv - Genetics 2023Quote: ... 1:500 to 1:1000 anti-rabbit (BioRad, 172-1019). Signal was detected using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
-
bioRxiv - Immunology 2021Quote: ... (BioRad Cl:A3-1) and goat anti-rat IgG cross-absorbed Alexa Fluor® 488 secondary antibody (ThermoFisher ...
-
Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... we separated beads from solution and transferred 44 μL of the supernatant (containing the DNA) into a new 96-well PCR plate (Bio-Rad HSP9631) for library preparation ...
-
bioRxiv - Molecular Biology 2021Quote: ... for the experiments done on 12-well plate) for each sample on SDS-PAGE using 4–20% Mini-PROTEAN® TGX gels (Bio-Rad), and immunoblotted as described (36) ...
-
bioRxiv - Molecular Biology 2019Quote: ... in a cartridge containing 20 µL of the reaction mix and 70 µL of Droplet Generation Oil for Probes (ddPCR™ 96-Well Plates #12001925, Bio-Rad). The entire emulsion volume was transferred from the cartridge to a 96-well PCR plate (Bio-Rad ...
-
bioRxiv - Microbiology 2019Quote: Pre-cultures were made from calibrated explants (5 mm) deposited on plate with solid Sisler medium previously covered with a sterile cellophane membrane (Bio-Rad, USA). The cultures were maintained 4 days at 20°C ...
-
bioRxiv - Bioengineering 2020Quote: ... Bioengineered intestinal tubules were exposed to reaction mixture for 30 min and absorbance was measured at 600 nm using colorimetric plate reader (iMARK™ microplate absorbance reader, Bio-Rad). Values are shown relative to medium exposed.
-
bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were centrifuged in v-bottom 96-well plates rather than microcentrifuge tubes and total protein concentration was determined using Protein Assay Dye (BioRad #500-0006) following standard protocols ...
-
bioRxiv - Neuroscience 2019Quote: ... Droplets were generated in a QX200 Droplet Generator and transferred to a standard 96-well PCR plate that was heat-sealed using foil sheets (Pierceable Foil Heat Seal, Bio-Rad, 1814040) and the PX1 PCR plate sealer ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... was used to very slowly aspirate generated droplet from the top row of the DG8™ Cartridge into the wells of a ddPCR semi-skirted 96-well plate (BioRad 12001925), and droplet generation repeated for all samples as above ...
-
bioRxiv - Microbiology 2020Quote: ... Colonization of SEΔΔΔ was quantified by dilution plating and counting colony forming units (CFU) on SASelect plates supplemented with 100 μg/mL D-alanine (BioRad, Hercules, CA). All experiments were done in triplicate.
-
bioRxiv - Neuroscience 2021Quote: ... Plates containing the amplified droplets were loaded into the QX200 droplet reader and results were analysed using QuantaSoft software™ (Bio-Rad).
-
ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... and the corresponding 5 μl of Primer Master-mix was added followed by sealing the plate with Microseal ® adhesive sealer (Bio-Rad). The 60S acidic ribosomal protein P0 (rplp0) ...
-
bioRxiv - Neuroscience 2020Quote: ... Amplifications were conducted using Bio-Rad SYBR green according to manufacturer’s instructions and plates were run in Bio-Rad CFX optical system (BioRad Laboratories, Hercules, CA). Primers were design to work at +60°C and to span an exon junction using online software at http://www.ncbi.nlm.nih.gov ...
-
bioRxiv - Microbiology 2022Quote: ... burgdorferi 16S rRNA by preparing samples in a 96-well plate using 5 µL of 2X SYBR Green Master Mix (Bio-Rad #1725121), 1 µL of each primer (Forward ...
-
bioRxiv - Plant Biology 2019Quote: ... All reactions were run in hard-shell skirted 96-well plates with a white top and clear wells and sealed with Microseal ‘B’ adhesive film (BioRad, Hercules, CA). A CFX-96 detection system (BioRad ...
-
bioRxiv - Cell Biology 2020Quote: ... after which the plates were air-dried for 30 min and developed using a K-screen and FX-Imager (Bio-Rad Laboratories).
-
bioRxiv - Biophysics 2019Quote: ... GFP+ cells were sorted from an unbiased FSC-SSC gate on a Sony SH800 cell sorter into 384-well hard-shell PCR plates (Bio-Rad HSP3901) containing .4 μl of lysis buffer ...
-
bioRxiv - Immunology 2021Quote: ... development buffer was added to each well and the plates were read every 10 minutes using a Model 550 Microplate Reader (Bio-Rad, UK) until the positive control reached a predetermined OD405 that was consistent across plates ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were allowed to adhere on plate/coverslips for 24 hours and then transfected with 20 nm of siRNA using siLentFect™ lipid reagent (Bio-Rad) for 24 hours.
-
Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The thermal shift assay was performed by following methods previously described,39 in a 96-well clear low-profile plate (Bio-Rad, #MLL9601) using CFX96 touch Real-Time PCR detection system ...
-
bioRxiv - Genetics 2022Quote: ... The agar plates were incubated at the indicated temperatures and time and imaged using the ChemiDoc™ Touch Imaging System (Bio-Rad).
-
bioRxiv - Synthetic Biology 2022Quote: ... The solid cultures were grown at 30ºC without shaking for 48 h and pictures of cell plates were taken using the colorimetric mode of Chemidoc Touch Imaging Systems (Bio-Rad, USA). All halo assays were conducted in three biological replicates.
-
bioRxiv - Microbiology 2022Quote: RT-qPCR was carried out in 384-well PCR plates (Framestar 480/384, 4titude, Dominique Dutscher) using the iTaq Universal SYBR® Green Supermix (Bio-Rad) and 0.5 μM primers with a LightCycler® 480 system (Roche Diagnostics ...
-
bioRxiv - Molecular Biology 2023Quote: ... The concentration of Evans blue was quantified spectrophotometrically at 620 nm using a plate reader (iMark microplate reader, Bio-Rad, CA, USA). To assess local vascular permeability in the skin ...