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Citations for Bio-Rad :
1701 - 1750 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... 1-5 μg of purified antigen or 20 μg protein extract were loaded in a precast 4-20% acrylamide gel (BioRad 456-1094). Gels were run for the first 20-30 min at 40V ...
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bioRxiv - Immunology 2020Quote: ... and 40ng of RNA for lung or 5-10ng of RNA for cells was converted to cDNA with iScript (Biorad, Hercules, CA). Real-time PCR was done on the cDNA with SYBR green master mix (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then centrifuged (2500 × g for 5 min) and pellets were resuspended in 100 μL of PAGE Laemmli Sample Buffer (Bio-Rad, 1610747) mixed with 50 μL TE 1X pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... around 400 ng of protein was loaded in each lane and 5 μL of Precision Plus Protein™ All Blue Prestained Protein Standards (Biorad, USA) was loaded in each gel as a marker ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was applied to a 5 ml MBPTrap HP Column (Cytiva, Marlborough, MA, USA) using an FPLC system (Bio-Rad NGC), pre-equilibrated and washed with 10 CV of buffer “A” ...
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 25-40μg of protein was run on 7.5-15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred using the Trans-Blot Turbo system (Biorad, Hercules, CA) to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2022Quote: ... was loaded and electrophorized with a standard of 5□μl of Precision Plus Protein™ All Blue Prestained Protein Standards (BioRad #1610373). For electrophoresis ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments was separated on 20% denaturing acrylamide gel (5% crosslinker, 7 M urea, 1X TBE buffer) using Criterion™ cell apparatus (Bio-Rad) at 300 V for 40 to 60 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of 10-fold diluted cDNA was combined with 5 µL of iQ SYBR Green Supermix (Bio-Rad, Cat# 170-8880) and 0.5 µL of each 10 µM forward and reverse primers ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples are prepared for SDS-PAGE by adding 5 μl of sample to 4 μl of tricine sample buffer (Bio-Rad 1610739) and 1 μl of 1 M DTT and heat denaturing at 70 °C for 3 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were prepared for SDS-PAGE by adding 5 μl of sample to 4 μl of tricine sample buffer (Bio-Rad 1610739) and 1 μl of 1 M DTT and heat denaturing at 90 °C for 10 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were run with 5 mM H2SO4 at 0.6 mL/min on an Aminex HPX-87H column (Biorad Inc., CA, USA) at 50℃ ...
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bioRxiv - Neuroscience 2023Quote: ... Five µL of each supernatant were run on a 5% to 20% gradient polyacrylamide gel (Wako) and then transferred onto an Immun-Blot® polyvinylidene fluoride membrane (Bio-Rad). Membranes were blocked with 5% skim milk in PBS containing 0.1% Tween-20 (PBST ...
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bioRxiv - Physiology 2023Quote: ... in duplicates in 384-qPCR plates (n = 5 plates) and qPCR were performed with a Biorad instrument (CFX-384, Biorad, Hercules, USA). We used Recombination Activating Gene 1 (RAG1 ...
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bioRxiv - Immunology 2023Quote: ... Blots were probed using primary antibodies in PBST (1X DPBS with 0.05% Tween-20 (BioXtra, catalog #P7949) with 5% Blotting Grade Blocker (Bio-Rad, catalog #1706404) for approximately 16 hours (overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... After the membranes were washed 3x during 5 minutes in TBST and the chemiluminescence was detected using Clarity™ Western ECL Substrate (Bio-Rad) and ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... heated up for 10 minutes at 95°C and centrifuged before loading onto a 10 well 5-20% gradient gel (BioRad 456-1093). The finished gels were blotted via semi dry method (20V ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA/gold mixture was resuspended in a Branson 2510 Ultrasonic Cleaner and 5 µL was spread onto each sterilized macrocarrier (#1652335, Bio-Rad, USA) and left to dry for 30 min.
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bioRxiv - Plant Biology 2023Quote: ... The samples were then separated by 6.5% (w/v) native polyacrylamide gels at 4 °C and the biotin-labeled probes was captured with ChemiDoc™XRS+ (Bio-Rad). The oligonucleotide probes used in this study are listed in Supplemental Data Set S1.
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bioRxiv - Molecular Biology 2023Quote: ... The resulting pellet was suspended in the lysis buffer and loaded onto a 5 ml IMAC column (Bio-Rad, CA, Hercules, USA) pre-equilibrated with a buffer A (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled at 95°C for 5 min and loaded into 4-12% Criterion XT-Bis-Tris polyacrylamide gels (Bio-Rad 3450125). Gel electrophoresis was performed in 1X MES running buffer (Bio-Rad 1610789 ...
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bioRxiv - Cancer Biology 2023Quote: ... was evaluated daily for 5 consecutive days by trypan blue staining with automated cell counting using a TC20™ counter (Bio-Rad). At day 0 ...
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bioRxiv - Plant Biology 2024Quote: Protein samples of whole cell extracts (5 μg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, US) and blotted onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were eluted from sepharose beads by boiling at 95 - 100 °C for 5 min with 40 µl sample buffer diluted from 4x sample buffer (Bio-Rad, 1610747) with Lysis Buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR was performed in 10 µl reactions containing 5 µl SsoFast EvaGreen Supermix with Low ROX (Bio-Rad; item # 1725211) diluted 1:2 ...
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bioRxiv - Biochemistry 2024Quote: ... and boiled at 95° C for 5 minutes before separation on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). In-gel fluorescence was imaged first ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The clarified lysate was loaded with a peristaltic pump (Hei-FLOW 06, Heidolph, Germany) on prepacked nickel-NTA columns (5 mL Nuvia IMAC Ni-Charged, Biorad, USA-CA) that were equilibrated before with buffer A ...
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bioRxiv - Microbiology 2024Quote: ... protein samples were boiled at 95 °C for 5 min and separated by a 4–20% Mini-PROTEAN® TGX Stain-Free™ SDS PAGE (BioRad) or 12 % Tris-Glycine SDS PAGA (Invitrogen ...
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bioRxiv - Zoology 2024Quote: Genomic DNA was extracted from the right hind leg of individual worker bee using a 5% (w/v) Chelex solution (Chelex®100; BIO-RAD), as followed by Walsh ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in agarose insert buffer and mixed 1:1 with 2% low-melting agarose (Bio-Rad). Gel inserts were solidified at 4°C for overnight and were stored in agarose insert buffer until analysis ...
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bioRxiv - Biochemistry 2020Quote: To visualize protein expression 1 μL of the TXTL reactions was mixed with 2 μL of water and 3 μL of 2x Laemmli loading dye (Bio-Rad, Hercules, CA, USA) and incubated at 90°C for 3 min ...
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bioRxiv - Neuroscience 2022Quote: ... anti-Ly-6B.2 (1:100, clone 7/4, BioRad, raised in rat) and anti-Nur77 (NR4A1 ...
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bioRxiv - Biochemistry 2022Quote: ... blots were imaged using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Biochemistry 2022Quote: ... Protein was detected using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Cell Biology 2023Quote: ... for 3 hours followed by 20 μM Nigericin for 3 hours (ICT9146, Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... The protein samples were mixed with LDS sample loading buffer at 4X concentration followed by addition of beta-mercaptoethanol (5%) (Bio-Rad, Hercules, CA). The resultant mixture was denatured at 95°C for 9 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-Time reactions were performed in triplicates using KAPA SYBR FAST qPCR Kit (2X) on QuantStudio 5 Real-time PCR system (Bio-Rad, CA, USA) following the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled at 100°C for 5 min prior to SDS-PAGE separation on 12% acrylamide Mini-protean TGX precast gels (Bio-Rad Laboratories, US) and Western blot analysis.
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bioRxiv - Microbiology 2020Quote: ... 2,5 or 5 μL of the eluate were analysed by RT-qPCR using CFX96 Real-Time Thermal Cycler (Bio-Rad, Hercules, California, USA) as described elsewhere [76] with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... was electroporated into cells at 5×106 cells/0.4 ml under conditions of 220V and 950 µF using a Gene Pulser™ (Bio-Rad, Hercules, CA) and plated on DMEM containing 10% FBS.
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bioRxiv - Cell Biology 2021Quote: ... cDNA was diluted 1:10 and 5 μl of the diluted cDNA was loaded in a total volume of 20 μl (SYBR Green Supermix (Bio-Rad 172-5124) and run on the Bio-RAD CFX Connect Real-Time System ...
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bioRxiv - Genomics 2022Quote: ... The new plate with droplets was sealed with a pierceable PCR foil at 180 °C for 5 seconds using PX1 PCR Plate Sealer (Bio-Rad, Cat. 1814000). The PCR reaction was then carried out using the C1000 Touch™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges, BIO-RAD 7324661), then dialyzed ...
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bioRxiv - Systems Biology 2022Quote: ... sample buffer (5 minutes at 95°C) and subjected to gel electrophoresis (4– 15% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and immunoblotting (polyvinylidene difluoride Transfer Membrane ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...
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bioRxiv - Biochemistry 2021Quote: ... were co-electroporated with 1.5 x 107 KH2 ES cells at 400 V and 125 μf using two pulses in a Gene PulserII (Bio-Rad, Hercules, CA). Recombination between FRT sites such that the entire plasmid is inserted within the PGK promoter and the ATG initiation codon upstream and in frame with the hygromycin resistance gene was used to select correctly targeted cells ...
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bioRxiv - Developmental Biology 2022Quote: ... and up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 172-5038). Quantitative real time-PCR was performed using Go-Taq qPCR Master Mix (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were denatured for 5 min at 95°C and separated by SDS-PAGE using precast 10% polyacrylamide 10-well (Bio-Rad, #456-1034) or 18-well (Bio-Rad ...