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Citations for Bio-Rad :
1651 - 1700 of 5509 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: A total of 5 μL of PCR amplicons were loaded onto a 1.5 % GelRed-stained agarose and the DNA bands were visualized using a gel documentation system (Gel Doc Ez Imager, Bio-Rad, CA, USA). PCR products were denatured at 95 °C for 10 min in the thermal cycler to avoid degradation by nucleases ...
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bioRxiv - Cancer Biology 2021Quote: ... NRF2 (CST, 12721S), KEAP1 (CST, 8047S), PRDX1 (CST, 8499S), GSTO1 (Thermo, PA5-83383)] in 5% BSA in TBS-T (Bio-Rad, 170-6435). Following incubation with primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: Cellular lysates were incubated at 95°C for 5 min and separated in 12% Mini-PROTEAN TGX Precast Protein gels (BIO-RAD, Cat #4561044). Proteins were then transferred to 0.45 μm PVDF membrane at 100V for 1 hr ...
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bioRxiv - Genetics 2021Quote: ... Samples were then centrifuged at 4°C for 5 min at 20,000 g and protein concentrations in the supernatants were measured using the Bradford assay (Bio-Rad Protein Assay Dye). Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the 30°C incubation step was shortened to 5 hr using a Bio-Rad C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). DNA concentrations were measured by Qubit ...
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bioRxiv - Microbiology 2021Quote: ... the 30°C incubation step was shortened to 5 hr using a Bio-Rad C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). DNA concentrations were measured by Qubit ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were heated 5 min at 95°C and separated on 4-20% Mini-PROTEAN® TGX Stain-Free™ Precast gels (Bio-Rad) or on home-made 7% polyacrylamide gel for OPA1 immunodetection ...
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bioRxiv - Molecular Biology 2020Quote: ... and the sample incubated at 90°C for 5 min and resolved by electrophoresis through a 4–20% gradient polyacrylamide/SDS gel (Bio-Rad Laboratories, 5671085). Next ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of tGFP was monitored for 5 h at 30°C using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).
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bioRxiv - Molecular Biology 2022Quote: ... followed by a final extension step at 68 °C for 5 min on the T100™ thermal cycler (Bio-Rad, Hercules, CA, USA). During protocol optimization ...
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bioRxiv - Microbiology 2022Quote: ... Expression and secretion samples were boiled for 5 min and then resolved on Mini-PROTEAN or Criterion™ TGX Stain-Free™ precast gels (Bio-Rad). For immunoblotting ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated with 50 µL/well of 5 µg/mL of mouse anti-bovine IFN-γ monoclonal antibody (mAb) (CC330, Bio-Rad Antibodies) by overnight incubation at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and 20 μL of the sample was mixed with 5 μL of a 5X Laemmli sample buffer containing 10% β-mercaptoethanol (Bio-Rad Laboratories). The sample was boiled for 5 min at 95 °C and loaded into a 12% polyacrylamide gel ...
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bioRxiv - Neuroscience 2023Quote: 1 µg BH RNAs and 5 µl bdEV RNAs were used to generate cDNA by iScript ™ Reverse Transcription Supermix for RT-qPCR (Bio-Rad 1708840). Expression levels of circRNAs were evaluated by qPCR using iTaq Universal SYBR ® Green Supermix (Bio-Rad 1725120) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were loaded onto Any kD™ Mini-PROTEAN® TGX™ gels and separated under reducing conditions for 5 minutes at 250 V (Biorad). Proteins were visualized by Coomassie Blue Staining (Sigma) ...
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bioRxiv - Physiology 2023Quote: ... 50µL from this mixture was transferred to a new tube containing 950µL of PBS and stored at 5°C for subsequent the cell counting (using a TC20 Automated Cell Counter, Bio-Rad, Solna, Sweden) 10 to 30 min later (when the mitochondrial measurements had started) ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 5 min at 95°C and loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Germany). Proteins were transferred on nitrocellulose membranes and membranes were blocked with 5% BSA in PBS-Tween ...
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bioRxiv - Biochemistry 2023Quote: Standard protocols were adopted for SDS-PAGE in 10% isocratic or 5%-15% gradient polyacrylamide mini-gels (Bio-Rad Laboratories S.r.l., Segrate, Italy), and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores ...
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bioRxiv - Microbiology 2023Quote: ... heated in water bath for 5 minutes at 99°C and analysed by SDS-PAGE using Any-kD Mini-PROTEAN TGX Stain-Free gels (Bio-Rad, California, USA) in a 2-minute run for sample clean-up purposes ...
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bioRxiv - Developmental Biology 2024Quote: ... and the reactions were stopped by boiling them at 95°C for 5 minutes after adding Laemmli sample buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol (Gibco 21985023) ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... denaturated at 95 °C for 5 min and separated on Bis-Tris polyacrylamide gels using the Mini-PROTEAN® Tetra Handcast System (Biorad, cat.no. 1658029FC). For visualization of total protein load ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Plant Biology 2023Quote: ... reactions were prepared in a 10 µL final volume containing 5 µL of 2x SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad), 0.125 µL of each specific primer pair at 250 nM ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... 50 µg of protein was denatured (95°C for 5 minutes) using lysis and Laemmli sample buffer (161-0737, Bio-Rad, with DTT). Samples were separated on a 10 % gradient gel (Criterion TGX Precast Gel ...
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bioRxiv - Neuroscience 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The gels’ stain-free dye was activated by a 5-min UV exposure and protein was transferred to Immun-Blot low-fluorescence PVDF membrane (Bio-Rad #162-0264) using Hoefer TE77X ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]). After removing the blocking buffer and re-applying PAP pen ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated at 4 °C for 30 min and heated at 37 °C for 5 min prior to analysis on precast “any KD” Mini-PROTEAN TGX (Bio-rad, Cat #4569033) polyacrylamide gels using 1x TBE as running buffer ...
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bioRxiv - Bioengineering 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... boiled for 5 min at 95° and resolved on 10 % or 7.5 % Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033, 4561036, 4561023, 4561026). The SDS-PAGE was performed at 100 V for 5 minutes and then 120 V for 1 hour 15 minutes using SDS running buffer (30 g Tris ...
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bioRxiv - Microbiology 2021Quote: The 20 μl RT-dPCR reaction mixture consisted of 5 μl One-Step RT-dPCR Advanced Supermix for Probes (Bio-Rad, Laboratories, Munich, Germany), 2 µl of Reverse Transcriptase (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... qRT-PCR was carried out using iQ™ SYBR® Green Supermix on an iQ™5 multicolor real-time PCR detection system (Bio-Rad). Expression of PCR transcripts was calculated using the 2-deltaCt with Yhwaz serving as the internal control gene ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed in triplicate in a total volume of 20 μL that included 2 μL of the template or standard plasmid and 250 nM of each primer and TaqMan (5’ hydrolysis) probe (the RT-qPCR reactions also included 0.5 μL of Bio-Rad iScript advanced reverse transcriptase). Thermocycling conditions for qPCR consisted of an initial 50 °C step for 2 min followed by 95 °C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 300nM final volume of each forward and reverse primer pair, 5 μL SYBR Green Supermix (50% total volume of reaction, rxn) (Bio-Rad Inc. Hercules, CA), and nuclease-free water (to make a final reaction volume of 10 μL) ...
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bioRxiv - Cell Biology 2021Quote: The supernatants of cell patches were collected after culturing under normoxic or hypoxic (5% O2) conditions and analysed using a fluorescence-dyed microsphere-based immune assay (Bio-Rad Laboratories, Hercules, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Biophysics 2022Quote: ... The re-suspended DNA droplet solution was then denatured at 95 °C for at least 5 min on a C1000 Touch™ Thermal Cycler from Bio-Rad (CA, USA). The aqueous solution consisted of 25% v/v SUV solution (containing 8mM lipids as prepared above ...
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bioRxiv - Microbiology 2020Quote: ... as per the manufacturer’s running conditions then activated for 5 min under UV to determine total protein (Bio-Rad ChemiDoc XR, Image Lab Software). Proteins were transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A 250 μL suspension of cells and 500 ng of double-digested (XbaI and KpnI) vector plasmid were incubated for 5–10 min on ice in a 4-mm Gene Pulser®/MicroPulser™ cuvette (BioRad, Hercules, CA). GenePulser XCellTM (BioRad ...
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bioRxiv - Immunology 2021Quote: ... washed in TBST and incubated with a recombinant human monoclonal anti-PAR antibody (1:2,500 in 5% nonfat dry milk-TBST; clones #19 and #21, highly specific for target-bound PAR, custom-designed at Bio-Rad AbD Serotec GmbH) (39 ...
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bioRxiv - Cell Biology 2022Quote: ... the mixes were boiled at 97°C for 5 min and loaded in a Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hércules, EE.UU) at 12% acrylamide/bis-acrylamide ...
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bioRxiv - Physiology 2022Quote: ... such that a total of 2 μg protein in 20 μL of buffer was loaded into wells of a Mini-PROTEAN TGX Stain-Free gel (5-14%) (Bio-Rad, Mississauga, ON, Canada). Protein ladder (5 μL of Precision Plus Protein™ All Blue Prestained Protein Standards ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression for the 5 marker genes was quantified by qPCR using SYBR Green PCR Master Mix (Bio-Rad Laboratories Inc., Hercules, CA, USA), in three independent samples per treatment ...
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bioRxiv - Cell Biology 2023Quote: ... They were then washed thrice with TBST (5 minutes/wash) at room temperature and signal developed for one minute using Clarity™ Western ECL substrate (Bio-Rad, Cat #: 1705060) or Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then washed thrice with TBST (5 minutes/wash) at room temperature and then incubated with HRP-conjugated goat anti-rabbit IgG (H + L) (Bio-Rad, Cat #: 170-6515) or goat anti-mouse IgG (H + L ...
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bioRxiv - Cell Biology 2023Quote: ... samples were boiled at 97°C for 5 min and run on a 12% Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hercules, USA) for 1 hour at 150V ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Microbiology 2023Quote: ... followed by 40 cycles (95°C for 5 seconds, 60°C for 40 seconds) on a CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA). The housekeeping gene GAPDH (PY17X_1330200 ...