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Citations for Bio-Rad :
1651 - 1700 of 10000+ citations for rno mir 137 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... were performed in biological triplicates with two technical repeats on the obtained cDNAs using a CFX96 Real-Time PCR Detection system (BioRad). Primer efficiencies and optimal primer concentrations were determined experimentally ...
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bioRxiv - Molecular Biology 2020Quote: ... Each reaction contained 3 technical replicates and were carried out using a CFX384 TouchTM Real-Time PCR Detection System (BioRad Laboratories Ltd. ...
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bioRxiv - Biochemistry 2022Quote: ... The qPCR reactions were carried out in a 96-well plate in CFX96 Real-time PCR Detection System (Bio-Rad). The primers were listed in Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... The quantification of target genes was performed on a CFX96 Touch Real-Time PCR Detection system (Bio-Rad Laboratories Inc.) with the following conditions ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative PCR was performed using TaqMan primers synthesized by Integrated DNA Technologies (Table S2) on a CFX96 C1000 Touch Real-Time PCR Detection System (BioRad). ZmCyanase expression was used to normalize among biological samples ...
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bioRxiv - Plant Biology 2021Quote: ... was used for converting the RNA into cDNA before amplifying target transcripts on a CFX Connect Real-Time PCR Detection System (Biorad). RT-PCR primers are listed in Supplemental Table 1.
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed with SYBR Green Master Mix (Thermo cat. 4309155) using three technical replicates per reaction in a CFX96 Touch Real-Time PCR Detection System (Biorad). TUBULIN1 was used as a normalizer for ChIP-qPCR experiments and PP2AA3 for RT-qPCR (Czechowski et al. ...
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bioRxiv - Plant Biology 2020Quote: ... and qPCR was conducted using Bio-Rad SYBR Green Supermix in CFX-Connect Real-Time PCR Detection System (Bio-Rad). The relative transcript level of genes was calculated following the ΔCt method (Schmittgen and Livak ...
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bioRxiv - Synthetic Biology 2021Quote: The PCIA extracted IP DNA was precipitated and quantified using a homemade EvaGreen based qPCR mix on a CFX Connect Real-Time PCR Detection System (BioRad). qPCR primers are listed in Table S4.
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bioRxiv - Microbiology 2019Quote: ... according to the manufacturer’s instructions in 10 µl reaction volumes and reactions were run on a CFX Real-Time PCR Detection System (BioRad). cas9 mRNA and 16srRNA were analyzed with the following primers ...
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bioRxiv - Microbiology 2021Quote: ... The qPCR was performed in triplicates using the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad, Hercules, USA). The qPCR conditions are given in Supplementary Tab ...
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bioRxiv - Microbiology 2019Quote: ... 1) 16S rRNA gene copies using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) and previously published primers targeting most bacterial 16S rRNA genes ...
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bioRxiv - Genetics 2020Quote: ... we measured GFP relative expression at a CFX-96 Touch Real-Time PCR Detection System (Bio-Rad; cat. no. 1855195) in triplicates for each sample with the following program ...
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bioRxiv - Physiology 2021Quote: ... Samples were run using the CFX96 Touch Real-Time PCR Detection System (running version 3.1 of the CFX Manager Software; Bio-Rad) and data all normalized to Gapdh and plotted relative to the time-independent average of the wild type samples using the 2−ΔΔCt method ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed using the intercalating dye SYBR green on the CFX96 Touch Real Time PCR Detection System (Bio-Rad). qPCR primers were designed to identify all isoform variants and were validated by IDT (Supplementary Table S3) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... We ran samples in duplicate for each gene on the same 384-well plate using a CFX384 Touch Real-time PCR detection system (BioRad). We validated all primers for this study by running a 10-fold serial dilution to determine amplification efficiencies (average ...
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bioRxiv - Plant Biology 2020Quote: ... were performed in biological triplicates with two technical repeats on the obtained cDNAs using a CFX96 Real-Time PCR Detection system (BioRad). Primer efficiencies and optimal primer concentrations were determined experimentally ...
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bioRxiv - Genetics 2019Quote: ... qRT-PCR was performed using the CFX Connect Real-Time PCR Detection System and analyzed with the CFX Manager Maestro Software (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... were performed with a CFX96 Touch Real-Time PCR Detection System and results were analyzed with the CFX Manager software (BioRad). Oligonucleotide primer sequences are listed in Table 1 ...
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bioRxiv - Immunology 2022Quote: ... and read fluorescence for 45 seconds at 60°C using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). The expression levels for each gene were then calculated per the 2-ΔΔCT equation [25].
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bioRxiv - Plant Biology 2022Quote: ... The qPCR was carried out using ChamQ Universal SYBR qPCR Master Mix (Vazyme) in the CFX96 Connect Real-time PCR Detection system (BioRad). Actin2 was used as the internal control and the sequences of the primers used for RT-qPCR is provided in Supplementary Table 1.
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bioRxiv - Synthetic Biology 2022Quote: ... All reagents except the double-stranded beacon were first assembled in a total volume of 9 μL and run at 37 °C in a CFX96 real-time PCR detection system (Biorad). Both WT SpCas9 and SpCas9-NG are highly active at this temperature ...
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bioRxiv - Plant Biology 2022Quote: ... and psbC genes was performed on a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Oslo, Norway) using the iProof High-Fidelity PCR Kit (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR with gene-specific primers was performed with the SsoAdvanced SYBR Green Supermix (Bio- Rad) on a CFX96 Real-Time PCR detection system (BioRad). Signals were normalized to the reference genes ACTIN2 using the ΔCt method and the relative expression of a target gene was calculated from the ratio of test samples to Col-0 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The reaction was kept at 37°C and recorded using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). To prevent evaporation the plate was sealed with a transparent sticker ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared assay plate was exposed to temperature increases from 25 ⁰C to 95 ⁰C with 1 ⁰C increments for 5 s each using a CFX Connect Real-Time PCR Detection System (BioRad). SYBR fluorescence was detected after every temperature increase ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then ran using the Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) with an internal standard curve constructed from a serial dilution of the E ...
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bioRxiv - Microbiology 2023Quote: ... PCR plates were then sealed and amplification was carried out with the CFX96 Touch Real-Time PCR Detection System thermocycler (BioRad) following a thermal program of continuous 65°C with fluorescence read every 30 s for 180 cycles ...
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bioRxiv - Plant Biology 2023Quote: ... following the users’ manual and the reaction was run in CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) under the setup with an initial denaturation at 95 C for 3 min ...
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bioRxiv - Microbiology 2023Quote: The cDNA was diluted to 1:20 and analysed using iQ5 Real time PCR detection system (BioRad, Hercules, California, USA) with SYBR Green detection system ...
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bioRxiv - Immunology 2023Quote: ... cDNA was amplified using the PerfeCTa SYBR green Supermix (Quanta bio) on a CFXConnect real time PCR detection system (Biorad). Results were analyzed using the accompanying software according to manufactures instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and subjected to thermo-cycling in a CFX96 Touch Real-time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). The conditions for thermal cycling were as follows ...
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bioRxiv - Cell Biology 2023Quote: ... USA) was used and reactions were carried out in a CFX Connect Real-Time PCR Detection System (Bio-Rad, USA). Reaction procedures were set to an initial heating step of 95 °C for 3 min as well as 40 cycles of denaturing at 95 °C for 10 s followed by combined annealing-extension at 60 °C for 40 s ...
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bioRxiv - Microbiology 2024Quote: ... China) were conducted in duplicate in a CFX-96 real-time PCR detection system (Bio-Rad Laboratories, Hercules, CA, USA). Using β-actin as the internal reference gene ...
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bioRxiv - Biochemistry 2024Quote: All fluorescence-based detection assays were carried out in a CFX96 touch real-time PCR system (Bio-Rad, CA, USA). The Cas12a ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were heated from 35 °C to 79°C at a rate of 0.3 °C per 15 s in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Fluorescence intensity was determined using excitation (λem=525/20 nm ...
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bioRxiv - Bioengineering 2020Quote: ... Quantitative real time RT-PCR (QPCR) were performed on the Opticon Continuous Fluorescence System (Bio-Rad Laboratories, Inc., Hercules, CA) using the QuantiTect SYBR Green RT-PCR kit (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed on a BioRad CFX connect Real-time system using universal SYBR® Green supermix (BioRad). Relative gene expression was calculated using the 2-ΔΔCT method ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative real time RT-PCR (QPCR) was performed on the Opticon Continuous Fluorescence System (Bio-Rad Laboratories, Inc., Hercules, CA) using the QuantiTect SYBR Green RT-PCR kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: Quantitative RT-qPCR was routinely performed on 0.5 µl cDNA in a CFX96 real-time PCR cycling machine (BioRad, USA) using H ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative mRNA expression was determined by real-time RT-PCR using iTaq Universal SYBR Green Supermix (Bio-Rad, 172-5124). Primers used were ...
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bioRxiv - Microbiology 2019Quote: ... PCR was conducted in a CFX96 RT-PCR Detection System (Bio-Rad, Munich, Germany) with the constitutively expressed control gene Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Genomics 2022Quote: QRT-PCR was conducted on a CFX-connect RT PCR detection system (Bio-Rad). The reaction used Genious 2X SYBR Green Fast qPCR Mix (ABclonal ...
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Transient Nodal signalling in left precursors coordinates opposed asymmetries shaping the heart loopbioRxiv - Developmental Biology 2019Quote: ... Quantitative PCR was carried out using a real-time PCR system (BioRad), and primers as listed in the Key Resources Table ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using SYBR Green (BioRad) and the topA gene was used for reference of data normalization.
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed using CFX96 Touch Real-Time PCR (Bio-Rad). Cycling conditions were 10 mins at 95°C followed by 40 cycles of 10 s at 95°C ...
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bioRxiv - Cancer Biology 2020Quote: ... on a MyiQ Single Color RT PCR Detection System (Bio-Rad). RT-PCR data was quantitated using the relative standard curve method and normalized against GAPDH intensity levels ...
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bioRxiv - Immunology 2023Quote: ... on an iQ5 RT-PCR detection system (BioRad, Hercules, CA, USA).
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bioRxiv - Genetics 2023Quote: ... on an iCycler RT‒PCR Detection System (Bio-Rad Laboratories, USA). ΔΔCT method was used for data analysis ...
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bioRxiv - Immunology 2019Quote: Quantitative PCR (qPCR) was performed as previously described (68) using a CFX96 Touch® Real-Time PCR Detection System (Bio-Rad Laboratories, CA, USA). For the quantification of E ...