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Citations for Bio-Rad :
1651 - 1700 of 10000+ citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
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bioRxiv - Biochemistry 2021Quote: ... Total proteins in the gel or on the membrane were visualized using Coomassie (InstantBlue, Sigma-Aldrich, scanned with the Odyssey or BioRad Chemidoc imagers) or Ponceau S staining ...
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bioRxiv - Synthetic Biology 2021Quote: ... The gel was then immediately placed into a Bio-Rad TransBlot® Turbo apparatus for protein transfer onto a nitrocellulose membrane (Bio-Rad #1704158). Since all samples were produced from the same batch of cell-free expression reaction mix or were of known concentration ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were fractionated via 8 ∼ 14% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using a Trans-blot apparatus (Bio-Rad, Hercules, CA). The PVDF membranes were then sequentially subject to blocking ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates (32 μg proteins/lane) were loaded to and separated on 10% SDS-PAGE gels and electrophoretically transferred to PVDF membrane (1620264, Bio-Rad, Hercules, CA). The membranes were blocked in 5% BSA/TBST (0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... An equal amount of total protein (20 – 50 μ per sample) was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620177) for blotting.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad, Cat #: 1704158EDU) on a Trans-Blot Turbo TM Transfer System (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal quantity of the protein was resolved in a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred to an Immun-Blot PVDF Membrane (Bio-Rad) for antibody incubation ...
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bioRxiv - Immunology 2023Quote: ... Same protein amounts were loaded onto 4-12% NuPAGE Gels and protein was transferred to PVDF membranes by using Trans-Blot® Turbo™ Transfer System (Biorad#17001917). Membrane was blocked with 5% milk-TBS ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein transferred to 0.45 mm polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo transfer system (BioRad Laboratories, Hatfield, PA, USA).
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Systems Biology 2023Quote: Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The gels’ stain-free dye was activated by a 5-min UV exposure and protein was transferred to Immun-Blot low-fluorescence PVDF membrane (Bio-Rad #162-0264) using Hoefer TE77X ...
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bioRxiv - Physiology 2024Quote: ... Then we separated proteins into 4-15% gradient SDS-PAGE gels and transferred them to PVDF immuno-blot membranes (Bio-Rad, Hercules, CA). The primary antibodies S6 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Membrane transfer was confirmed through UV-imaging of membrane (BioRad ChemiDoc ImageLab software). The membranes were incubated with 1:5,000 monoclonal ANTI-FLAG M2 antibody produced in mouse (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... SARS-CoV-2 RT-qPCR reactions (10 µL) using the iTaq Universal Probe One-Step Kit (BioRad) and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713 ...
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bioRxiv - Microbiology 2019Quote: ... and genomic DNA was obtained by using InstaGene matrix as described by the supplier (Bio-Rad Laboratories, United Kingdom). CAI and CAII complete sequences from the different strains were amplified by PCR with oligonucleotides BS192_0456.F/R and BS191_1911.F/R respectively ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Genomic DNA was isolated from tail clippings or liver tissue using the Chelex Instagene-matrix extraction protocol (Bio-Rad).
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bioRxiv - Biophysics 2019Quote: ... All custom-made antibodies were purified from serum with an antigen-coupled matrix (Affi-Gel 10 or 15, Biorad). For western blot analysis of other proteins ...
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bioRxiv - Microbiology 2023Quote: ... The protein-containing cell culture supernatant was passed over 0.5ml of a Strep-Tactin Superflow (IBA Lifescience) matrix in a gravity flow Omniprep column (BioRad). Bound protein was washed with approximately 50ml phosphate buffered saline pH 7.4 (PBS ...
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bioRxiv - Microbiology 2022Quote: ... coli cells were transformed using the Pulser XCell system (Bio-Rad, USA), with the following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 µg of WCE were separated on mini protein TGX stain-free gel and electroblotted using the PVDF transfer membrane (Bio-Rad, Hercules, CA, USA). The ChemiDoc™ MP imaging system (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 μg proteins of the cell line were exposed to a 10% SDS-PAGE gel and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: Proteins were transferred from gels to PVDF membranes using a Trans-Blot® Turbo™ Transfer System and associated accessories (Bio-Rad Laboratories, Inc.). Membranes were probed with anti-Strep-II tag and anti-CB2 antibodies according to manufacturer instructions.
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bioRxiv - Bioengineering 2019Quote: ... the protein bands were visualized upon few minutes of incubation of the membranes with Bio-Rad Clarity Western ECL Substrate (Bio-Rad#1705061, Cressier, Switzerland). Blots were imaged using ChemiDoc imaging system for chemiluminescence assays with 0.1 s exposure ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were transferred on a PVDF membrane according to the manufacturer’s protocol (Trans-Blot® Turbo™ Rapid Transfer System, BIORAD, Les-Ulis, France) using a small protein transfer program ...
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bioRxiv - Neuroscience 2023Quote: ... A 5 μL aliquot of the aSyn protein not incubated or incubated for different periods under aggregating conditions was transferred to a nitrocellulose membrane (Bio-rad®; 0.45 μm). The sample was then blocked with OdysseyTM blocking buffer for 60 min at 22° C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An equal amount of total protein was resolved by a 10% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked and incubated with either anti Phospho-(Ser/Thr ...
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bioRxiv - Molecular Biology 2022Quote: 20µg of protein extracts or 10% Co-IP samples were resolved on 10-12% SDS–PAGE and proteins were transferred to PVDF membranes using a semi dry Western blot system (Bio-Rad Trans-Blot SD). Membranes were blocked in 5% non-fat milk in PBS-T (1x PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein were resolved by sodium dodecyl sulfate-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA, USA). Blots were probed with the indicated primary antibodies listed in Extended Data Table 4 ...
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bioRxiv - Genomics 2022Quote: ... XCell II Blot module was used for wet electrophoretic transfer of proteins from SDS-PAGE gel to nitrocellulose membrane (Biorad, Cat # 1620145, 0.45 μm) in Tris-Glycine Electroblotting buffer (National Diagnostic ...
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bioRxiv - Molecular Biology 2023Quote: ... After gel migration proteins were transferred to Amersham nitrocellulose Protran Western blotting membranes (GE10600001) using semi-dry transfer (SEMI DRY BLOT APPARATUS, Bio-Rad, cat. no.1703940). Blots were blocked in TBS-T and 5 % milk and probed with relevant antibodies overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was transferred to PVDF membrane (Amersham 10600022) using semi-dry transfer apparatus using the ‘mixed MW’ setting (Bio-Rad trans blot turbo). Membranes were blocked for 1 hour in 5% powdered milk in TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... Cellular proteins were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis before being electroblotted onto polyvinylidene fluoride membranes (#1704156, Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with the following primary antibodies for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of proteins (20 μg) were separated by SDS-PAGE and then transferred onto nitrocellulose membranes (ref 1704271, Bio-Rad, Hercules, California, USA). Membranes were incubated in blocking buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores) using a Trans Blot Turbo system (Bio-Rad Laboratories S.r.l., Segrate, Italy) according to manufacturer’s instructions ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: Cell or tissue lysates (10–30 μg protein) or immunoprecipitated samples were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against leptin (Cat# ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of proteins (20 μg) were separated by SDS-PAGE and then transferred onto nitrocellulose membranes (ref 1704271, Bio-Rad, Hercules, California, USA). Membranes were incubated in blocking buffer ...
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Genetic analysis argues for a coactivator function for the Saccharomyces cerevisiae Tup1 corepressorbioRxiv - Genetics 2021Quote: ... A nitrocellulose membrane (Bio-Rad) was used for western transfer with standard methods ...
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bioRxiv - Immunology 2019Quote: ... PVDF membrane was from BioRad Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... LF-PVDF membranes (Bio-rad) were used to transfer the proteins at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... the PVDF membranes (Bio-Rad) were incubated with blocking buffer (5% skim milk in Tris-buffered saline with Tween 20 ...
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bioRxiv - Microbiology 2020Quote: ... A PVDF membrane (Bio-rad) was hydrated with methanol for 15-30 seconds and pre-wet alongside a stack of blotting paper for 3-5 minutes in 1X Transfer buffer ...
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bioRxiv - Microbiology 2021Quote: ... the nitrocellulose membranes (Bio-Rad) were treated with 20% blocking buffer (Li-Cor Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... onto nitrocellulose membranes (BioRad 1620112). Membranes were blocked with 6% milk (BioRad 1706404 ...
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bioRxiv - Genomics 2022Quote: ... transferred onto PVDF membranes (BioRad), blocked with 5% w/v powdered milk in TBST buffer (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... transferred to nitrocellulose membranes (BioRad), and probed using the following specific antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... transferred to PVDF membranes (Biorad), and then subjected to standard immunoblotting procedures as previously described (Tubbesing et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... PVDF membrane (Biorad 162-0177) was used for Western blotting of separated protein samples ...