Labshake search
Citations for Bio-Rad :
1501 - 1550 of 10000+ citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: Tissue or cell extracts equivalent to 20 μg total protein were resolved in 10% SDS-PAGE gels followed by electrophoretic transfer onto a nitrocellulose membrane (Bio-Rad) in Tris-glycine buffer ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins were transferred to a Polyvinylidene difluoride (PVDF) membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad). Detection of GFP was carried out with a rabbit polyclonal anti-GFP antiserum (Bio-Rad ...
-
bioRxiv - Molecular Biology 2021Quote: ... samples were subjected to SDS-polyacrylamide gel electrophoresis and proteins were blotted onto nitrocellulose membranes using the Mini Trans-Blot Cell system (Bio-Rad).
-
bioRxiv - Synthetic Biology 2021Quote: ... Total proteins were separated in SDS-PAGE and transferred to a PVDF membrane using Transblot turbo (Bio-Rad Laboratories, CA, USA). After blocking the membrane with 0.3% skim milk in TBS-T ...
-
bioRxiv - Microbiology 2020Quote: ... proteins were blotted onto nitrocellulose membranes (Whatman, Maidstone, United Kingdom) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) under conditions suggested by the manufacturer ...
-
bioRxiv - Microbiology 2020Quote: ... Protein bands for both of the gels were transferred to polyvinylidene fluoride (PVDF) membranes (Immun-Blot® 0.2 μm, Bio-Rad) using semi-dry electroblotting system (Trans-Blot® TurboTM Transfer System ...
-
bioRxiv - Biophysics 2020Quote: ... 15 μg of proteins were loaded onto gels prior to conducting SDS-PAGE and transferring onto polyvinylidene difluoride membranes (Bio-Rad). Membranes were then incubated with monoclonal antibodies against Cyclin B1 (PC133 ...
-
bioRxiv - Physiology 2019Quote: ... The total protein (about 50 μg) was separated in an SDS-PAGE (10 %) and transferred onto nitrocellulose membrane (Bio-Rad, America). The membranes were blocked with TBST (137 mM NaCl ...
-
bioRxiv - Developmental Biology 2019Quote: ... were used to standardize amount of total protein loaded on a gel 12% polyacrylamide gel was then transferred to polyvenyidene fluoride (PVDF membrane, Bio-Rad). 5% nonfat dry milk in PBST (Phosphate buffered saline with Tween 20 ...
-
bioRxiv - Genetics 2019Quote: ... Separated proteins were transferred to PVDF membrane at 350 mA constant for 90 minutes using the Mini-TransBlot system (Bio-Rad). Transfer was validated by ponceau stain (Sigma) ...
-
bioRxiv - Cell Biology 2020Quote: Transfected cells were lysed in RIPA buffer on day 4 and proteins extracted were separated by 10% - 15% SDS polyacrylamide gel and transferred to Nitrocellulose membranes (Bio-Rad) at 100V for 1 hour and blocked for 1 hour with 5% non-fat milk (Bio-Rad ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein content was determined by BCA assay (Pierce) and proteins were separated by SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad). Membranes were blocked ...
-
bioRxiv - Bioengineering 2021Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Cytiva) using a Trans-Blot SD Semi-Dry Electrophoretic Cell (Bio-Rad) with 1.5 mA / cm2 membrane for 1 h ...
-
bioRxiv - Biochemistry 2021Quote: Equal amount of protein was loaded and resolved on 10% polyacrylamide gel and transferred onto the PVDF membrane supplied by Bio-Rad, USA ...
-
bioRxiv - Biochemistry 2021Quote: ... 80 μg of total protein for each sample was resolved by SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad). Membranes were blocked in Tris-buffered saline + 0.1% (v/v ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins (20 μg – 40 μg depending on the target protein) were resolved by 10%-12% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (BioRad, USA). The membranes were blocked with 5% non-fat milk for 2 h at RT and subsequently probed with specific primary antibodies overnight at 4°C (Table EV2) ...
-
bioRxiv - Cancer Biology 2021Quote: ... the equal quantity of protein (40 µg) was resolved on 10 % polyacrylamide gel and transferred onto the PVDF membrane supplied by Bio-Rad, USA ...
-
bioRxiv - Genetics 2021Quote: ... Equal amounts of proteins were resolved by polyacrylamide-SDS gel electrophoresis (PAGE) and transferred to nitrocellulose membrane (Bio-Rad, Hercules, CA) using Semi-Dry Transfer Cell (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The resolved proteins were transferred to Immuno-Blot® PVDF membranes using a Trans-Blot® Turbo™ Transfer System (BioRad). Membranes were incubated at 4°C overnight in primary antibodies against GRP78 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of total protein were separated on SDS-PAGE gel by electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad). Target proteins were detected using the following antibodies ...
-
bioRxiv - Neuroscience 2021Quote: ... Bolt 4-12 % Bis-Tris Plus Gels and Bolt MES SDS Running Buffer 20X were used for protein separation before transfer to nitrocellulose membranes (Transblot Turbo Transfer Pack, Bio-Rad) using a Transblot Turbo 0.2 μm (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Equal masses of protein (5 ug – 40 ug) were separated by 4—15% of SDS/ PAGE and were transferred onto nitrocellulose membranes (Bio-Rad) for protein blot analysis ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were loaded to each lane on a 4-20% gradient SDS/PAGE gel and transferred to nitrocellulose membranes (Bio-Rad). Signals were developed with HRP-conjugated secondary antibodies and SuperSignal West Pico Chemiluminescent Substrate (Pierce) ...
-
bioRxiv - Neuroscience 2021Quote: ... Thirty micrograms of total protein from each sample were loaded on 8% SDS-PAGE and transferred to PVDF membranes (Bio-Rad).
-
bioRxiv - Immunology 2021Quote: ... Cell lysate proteins were resolved on a 4–12% gradient SDS-PAGE gel and transferred to Trans-Blot nitrocellulose membrane (Bio-Rad). Blots were incubated with polyclonal goat anti-mouse TLR2 Ab (R&D Systems ...
-
bioRxiv - Immunology 2021Quote: ... Tissue lysates were mixed with 2x Laemmli sample buffer and incubated at 100°C for 5 min and equal amounts of protein were loaded into a 4-15% polyacrylamide gel for SDS/PAGE and transferred onto a nitrocellulose membrane (Bio-Rad). Membranes were incubated with the following primary antibodies ...
-
bioRxiv - Immunology 2022Quote: ... Separated proteins were transferred to a PVDF membrane using the Trans-Blot Turbo RTA Transfer Kit (Bio-Rad Laboratories, Feldkirchen, Germany) according to the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2022Quote: ... and proteins were then transferred to a nitrocellulose membrane using a Trans-Blot Turbo Nitrocellulose Transfer Pack and Transfer System (Bio-Rad). The membrane was blocked for 1 h with 5% skim milk in PBS supplemented with 0.05% Tween 20 (PBS-T ...
-
bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (BioRad, California, USA), which were blocked with 5% skim milk for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were heated in reducing Laemmli sample buffer at 95°C and resolved in SDS-PAGE protein gel and transferred to PVDF membrane (Bio-Rad). Membranes were blocked using 5% BSA (Sigma ...
-
bioRxiv - Cell Biology 2022Quote: ... Total protein was resolved on 10% SDS–polyacrylamide gel electrophoresis gels and transferred onto PVDF membranes (Bio-Rad, catalog no. 1704156) by Trans-Blot Turbo (Bio-rad) ...
-
bioRxiv - Systems Biology 2022Quote: ... semidry electrotransfer of proteins to a PVDF membrane was carried out in a Trans-Blot Turbo Transfer System (Bio-Rad, USA). The membrane was incubated in blocking buffer (PBST ...
-
bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein extracts were loaded and separated on a 10% SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad). Membranes were blocked with a 3% BSA in TBS-T (Tris-Buffered Saline with Tween-20 containing in mM ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein (30 μg) was resolved on a 12% SDS-PAGE and transferred to a 0.45 μm nitrocellulose membrane (BioRad Cat# 1620115). The membranes were probed with anti-MAG ...
-
bioRxiv - Physiology 2022Quote: ... after which the separated protein was transferred to a polyvinylidene fluoride (PVDF) membrane in a Trans-Blot Turbo System (Bio-Rad) at 25 V (1.3 A ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 60 μg of each protein sample was loaded on the polyacrylamide gel and then transferred to polyvinylidenedifluoride (PVDF) membrane (TransBlot Turbo, Bio-Rad) with semi-dry transfer (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... The resulting lysate was resolved on SDS-PAGE gel and protein was blotted to nitrocellulose membrane (Li-COR #926-31902) with mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for an hour in Odyssey Blocking Buffer (TBS ...
-
bioRxiv - Bioengineering 2022Quote: ... Equal amounts of protein extracts were resolved by 10%-12% SDS-PAGE and electrotransferred onto a polyvinylidene membrane (Bio-Rad, USA). After blocking in Tris-buffered saline plus 5% (w/v ...
-
bioRxiv - Bioengineering 2022Quote: ... SDS page was performed on the samples and the proteins were transferred to nitrocellulose membranes using wet transfer (Bio-Rad Laboratories). The membranes were blocked with PBS with 0.1% Tween-20 (PBST ...
-
bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred from the unstained gel to polyvinylidene fluoride membranes using the Trans-Blot turbo transfer system (Bio-Rad Laboratories). An iBind™ Flex system (Invitrogen/ThermoFischer ...
-
bioRxiv - Cell Biology 2023Quote: ... Approximately equal loads of extracted protein were subjected to SDS-PAGE and transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Five micrograms of total protein were resolved by SDS-PAGE on a 10% gel under reducing conditions and transferred to PVDF membranes (Bio-Rad). The blots were blocked in TBS containing 5% non-fat milk for 30 mins and incubated with SARS-CoV-2 nucleocapsid protein antibody (1:1000 ...
-
bioRxiv - Genetics 2023Quote: ... The gels were run until proper band separation was achieved and the protein was transferred onto a 0.2 µm nitrocellulose membrane using the wet-transfer method (Bio-Rad Laboratories). The membranes were blocked with Odyssey Blocking Buffer (LiCOR Biosciences ...
-
bioRxiv - Pathology 2023Quote: ... total proteins were extracted and examined as previously described.59 The blotting was performed on the PVDF membrane (Bio-Rad, USA) and imaged with the Western Blotting Detection Kit (Advansta ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and transfers of proteins to .45uM nitrocellulose membranes were performed using the Protean 2 or 3 systems (Bio-Rad) and protocols provided by the manufacturer ...
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred onto PVDF membranes applying the Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). Membranes were blocked with 5% BSA or 5% NFDM in 1X TBS-T buffer and incubated with specific primary and secondary antibodies ...
-
bioRxiv - Microbiology 2023Quote: Total protein extracts (5x106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
-
bioRxiv - Immunology 2023Quote: ... 8 µg protein per condition were separated by SDS-PAGE and blotted onto a 0.2 µm nitrocellulose membrane (Bio-Rad, #1704158) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
-
bioRxiv - Physiology 2023Quote: ... The protein extract (40μg) was separated on 8 or 10% polyacrylamide mini gel and transferred to a PVDF membrane (Bio-Rad, USA). Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS ...