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Citations for Bio-Rad :
1651 - 1700 of 1714 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... were used as secondary antibodies for 1 hour at the room temperature and revealed with the addition of Clarity Western ECL reagent (Bio-Rad Laboratories Ltd). ChemiDoc MP Imaging System (Bio-Rad Laboratories Ltd ...
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bioRxiv - Plant Biology 2021Quote: ... A second polyclonal antibody conjugated with alkaline phosphatase was used for immunodetection (1:1000) at wavelength 405 nm (BioRad Microplate Reader model 680) via the substrate p-nitrophenyl-phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... Immunocomplexes were visualized with horseradish peroxidase-conjugated secondary antibodies and detected with a Clarity Western ECL substrate (Bio-Rad #170-5061, Hercules, CA), and images were acquired on a chemiluminescent imager (Bio-Rad Chem-Doc Imaging System).
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bioRxiv - Physiology 2020Quote: ... The following antibodies were selected and tested on half of each cornea: anti-CD31 mouse monoclonal IgG1 targeting vascular endothelial cells (BioRad, Kidlington, Oxford, England), anti-Neutrophil rabbit polyclonal IgG targeting neutrophils (LifeSpan BioSciences ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 h incubation with the respective secondary antibodies: StarBright700 anti-rabbit (10000068187, 1:5000) or StarBright700 anti-mouse (10000068185, 1:2500) both from BioRad (Hercules, CA, USA). Four washes with TBS-T were performed after incubation with secondary antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... A goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) antibody with HRP conjugate (1706515 and 1706516, respectively, Bio-Rad, Hercules, CA) was used as a secondary detection antibody ...
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bioRxiv - Immunology 2020Quote: ... labelled with viability stain and surface antibodies as above and fixed and permeabilised using Leucoperm A and B reagents (Bio-Rad, Watford, UK). Cells were labelled intracellularly with antibodies (Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... Immunocomplexes were visualized with horseradish peroxidase-conjugated secondary antibodies and detected with a Clarity Western ECL substrate (Bio-Rad #170-5061, Hercules, CA), and images were acquired on a chemiluminescent imager (Bio-Rad Chem-Doc Imaging System).
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bioRxiv - Cell Biology 2021Quote: ... Appropriate secondary antibodies coupled to horseradish peroxidase were detected by enhanced chemiluminescence using ClarityTM Western ECL Blotting Substrate (Bio-Rad, Hercules, CA, USA). Resulting protein bands were quantified and normalised to each lane’s loading control using the ImageStudio Lite software (LI-COR) ...
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bioRxiv - Cell Biology 2022Quote: ... Then membranes were washed 3 times with TBS-T and incubated with goat anti-mouse or anti-rabbit IgG HRP-conjugated secondary antibody (1:10000 in 5% BSA TBS-T, Bio-rad; #1706515, #1706516) for 1 h ...
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bioRxiv - Genetics 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated antirabbit (1: 2500, 170-6515) or antimouse (1: 5000, 170-6516) IgG secondary antibodies (BioRad, Hercules, CA, USA). Proteins were detected using the SuperSignal chemiluminescent substrate kit (Pierce).
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times in TBST prior to incubation with secondary antibody (1/5000 dilution horseradish peroxidase (HRP)-conjugated anti-mouse (Bio-Rad, 170-6516) or anti-rabbit (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were washed again three times with TBS-T followed by the addition of primary antibodies (neat antivenom and normal horse IgG [BioRad] as negative control) in duplicate ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes underwent three times with PBS-T and were then subjected to incubation with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, CA, USA) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in cell lysate was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Catalogue #: 12004167; Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins bound by both primary and secondary antibodies were visualized by chemiluminescence after incubating the blot with Clarity Max Western ECL Blotting Substrate (Bio-Rad, Hercules, CA). Blot images were acquired on a ChemiDoc gel imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used were Immun-Star goat anti-rabbit (GAR)-HRP Conjugate (Cat # 170-5046, or Cat#170-6515; Bio-Rad Laboratories, USA). Clarity Western ECL Substrate (cat #170-5061 ...
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bioRxiv - Molecular Biology 2023Quote: ... primary antibodies and proteins were detected by incubation at a 1:5000 dilution with secondary horseradish peroxidase (HRP)- conjugated antibodies (#170-6516, goat anti-mouse and 170-6515; goat anti-rabbit from Bio-Rad, Hercules, USA). The reactive protein bands were visualized using the WesternBright ECL HRP substrate (Advansta ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with horseradish peroxidase (HPR)-conjugated secondary antibody and visualized by chemiluminescence using a horseradish peroxidase substrate (Bio-Rad, Cat. 170-5061). Quantification was performed using ImageJ.
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated with respective horseradish peroxidase (HRP) conjugated secondary antibodies for two hours and imaged using ChemiDoc (Bio-Rad, California, USA) after reacting with HRP substrate ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated for 1 hour with fluorescent-tagged secondary antibody diluted in PBST (Goat anti-Rabbit IgG StarBright™ Blue 700 or Goat anti-Mouse IgG DyLight™ 800, Bio-Rad). Finally ...
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bioRxiv - Immunology 2023Quote: ... The expression of immune system markers were mostly studied with inmunohistochemistry using the following antibodies: mouse anti rat CD68 (dilution 1:300 BIORAD, ref. MCA 341R) for monocytes/macrophages ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti mouse #1721011; Bio-Rad or goat anti rabbit #1706515; BioRad) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were diluted in blocking buffer (Iba1, 1:1000, Wako cat no 019-19741; CD68, 1:400, Bio-Rad cat no MCA1957) and incubated overnight at 4°C with mild agitation ...
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bioRxiv - Cell Biology 2024Quote: Proteins were detected by chemiluminescence following Western blotting using HRP conjugated secondary antibodies of anti-mouse (Bio-Rad # 170–6516; dilution 1:10,000), anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Microbiology 2024Quote: ... membranes were incubated in an appropriate secondary antibody: anti-Rabbit IgG-HRP Conjugate #1706515 and anti-Mouse IgG (H + L)-HRP Conjugate #1706516 (Bio-Rad, Hercules, CA). Image Quant TL Toolbox v8.1 software (Amersham Bioscience ...
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bioRxiv - Developmental Biology 2024Quote: ... Goat anti-mouse antibody conjugated to horseradish peroxidase (HRP) was used as a secondary antibody (1:2000) for chemiluminescence detection (Clarity Western ECL Substrates, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used (see also Supplementary Table 1 for details): mouse monoclonal IgG1 anti-βCYA (Bio-Rad, clone 4C2, RRID:AB_2571580), mouse monoclonal IgG2b anti-γCYA (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Then washed and incubated with the following peroxidase-conjugated secondary antibodies at room temperature for 45 min: goat anti-rabbit IgG HRP (1:3000; Bio-Rad, California, USA) and goat anti-mouse IgG HRP (1:3000 ...
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bioRxiv - Microbiology 2024Quote: ... PCR products and plasmids were transfected into T7 expressing Baby Hamster Kidney Cells (22) (BHK: BSRT7) using transfections and antibodies as in the confocal section but using a Zoe fluorescent microscope (Bio-Rad, Hercules, CA). All transfections were carried out using X2 polymer (Mirus ...
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bioRxiv - Physiology 2024Quote: ... the membrane was incubated with HRP-conjugated secondary antibodies against mouse (Cat No. 1721011, Bio- Rad Hercules, CA) and rabbit (Cat No. 1706515, Bio-Rad, Hercules, CA) at room temperature for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... Cocktails of antibodies were prepared (α-PanT Lymphocytes:APC [Bio-Rad, MCA751APC], α-CD45:AF750 [Bio- Techne, NB100-65362AF750], α-CD4:PE [Bio-Rad, MCA749PE] ...
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bioRxiv - Microbiology 2024Quote: ... followed by IR800-conjugated goat anti-mouse immunoglobulin (LiCor 926-32210; 0.1 μg/ml) and DyLight680-conjugated mouse anti-rabbit GAPDH antibody (BioRad MCA4739D680; 0.3 μg/ml). Membranes were imaged using a LiCor Odyssey CLx imager.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were immunoprobed using an HRP-conjugated secondary antibody (dilution 1:3000) and chemiluminescent substrate (Clarity Western ECL substrate, Bio-Rad, Hercules, CA, USA). Blot image acquisition was performed using a Molecular Imager® Gel Doc™ XR+ system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... The plate was washed 3 times prior to adding 50 μl of 1:1000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Bio-Rad, Cat# 172-1011) to the plate and incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent dye-conjugated secondary antibodies were used at 1:3000 and the proteins were detected using ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 h with either anti-mouse or anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Bio-Rad Laboratories, Hercules, CA). Bands were visualized and subjected to densitometric analysis using an Odyssey Fc system (LI-COR Biotechnology ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... After overnight incubation membranes were washed three times with 1x TBST for ten minutes per wash and then incubated with a secondary goat anti-rabbit antibody (BioRad Cat#1706515, 1:10000) for one hour with rocking at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Chemiluminescent detection was performed using film or a VersaDoc CCD camera with Clarity™ Western ECL Substrate and horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA) the next day ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times with 1X PBS for 5 min and incubated with goat anti-rabbit IgG-alkaline phosphatase-conjugate secondary antibody (Bio-Rad, 1:3000 dilution) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Nitrocellulose-bound primary antibodies were detected with anti-rabbit IgG horseradish peroxidase-linked antibody using the Clarity Western Peroxide and Luminol/Enhancer reagents (Bio-Rad Laboratories, CA, USA).
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bioRxiv - Developmental Biology 2023Quote: ... The membranes were washed with TBST three times and then incubated with a secondary antibody for 1 h before using Luminol/enhancer solution and ClarityTM Western ECL Substrate (Bio-Rad Laboratories, Inc. US). Western blot images were scanned by using Gel Doc XR system (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2023Quote: Vaginal mucosa cells and splenocytes were analyzed by flow cytometry using the following antibodies: mouse anti-guinea pig CD8 (clone MCA752F, Bio-Rad Laboratories, Hercules, CA), mouse anti-guinea pig CD4 (clone MCA749PE ...
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bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...
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bioRxiv - Microbiology 2023Quote: ... Revelation was performed using a second antibody coupled to the HRP (anti-rabbit or anti-mouse IgG conjugated to horseradish peroxidase, Bio-Rad, 1:5,000 dilution) and detected using the Clarity Western enhanced-chemiluminescence (ECL ...
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bioRxiv - Physiology 2024Quote: ... for 24h for paraffin embedding. Adjacent full footprint sections were immunostained for glucagon (in-house antibody; Dr. Michael Appel) and insulin (Bio-Rad cat. 5330-0104G) using a standard ABC peroxidase protocol (Vector Labs cat ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... Both primary antibodies were diluted 1:5,000 in PBST with 0.2% milk and subsequently conjugated with α-rabbit antibody-HRP conjugate (BioRad Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate #1705046 ...