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Citations for Bio-Rad :
1601 - 1650 of 1714 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed twice with PBS and incubated with secondary antibodies (Desmin and MYOD1: anti-rabbit-HRP, BioRad 170-6515; Myogenin: anti-mouse HRP, BioRad 170-6516) 1:500 in PBS-1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG HRP conjugated secondary antibody was used to detect the protein bands by chemiluminescence (Clarity ECL substrate, Cat no.170-5061, Biorad, California, USA).
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bioRxiv - Microbiology 2021Quote: ... The purity of the eluted fractions was assessed by SDS-PAGE and Western blot using a mouse anti-histidine antibody (BioRad, AbD Serotec).
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bioRxiv - Immunology 2021Quote: ... Secondary antibodies were added according to manufacturer’s protocol and membranes were imaged using chemiluminescence reagent Clarity Western ECL Substrate (BioRad laboratories, Hercules, CA) and a BioRad ChemiDoc XRS system ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were detected by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat anti-mouse IgG (BioRad ...
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bioRxiv - Immunology 2021Quote: ... Livers were paraffin-embedded and immunohistochemistry was performed on 5-micrometer sections using F4/80 antibody clone Cl:A3-1 (BioRad Cat. No. MCA497) and the VECTASTAIN Elite ABS HRP kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: Autaptic murine striatal neurons (DIV 15-18) were incubated with 1 μg/ml human GABAAR mAb or control antibody (anti-CD52, Bio-Rad, #HCA175) at 37°C for 24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C and the supernatant incubated during 4 h at 4°C with 50 µl of V5 magnetic beads (20 mg/mL of Dynabeads® M-280 Tosylactivated, Invitrogen, coupled with Anti V5-Tag Antibody, clone SV5-Pk1, Bio-Rad). The unbound fraction was discarded and the beads washed 2 times with 1ml of IP Buffer and additionally twice more with 1 ml ice cold PBS + protease inhibitors ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were visualized with goat anti-mouse or goat anti-rabbit IgG secondary antibodies (1:5000) diluted in 2% Omniblot milk (AmericanBio) in 1X TBST using a chemiluminescence detection system (BioRad ChemiDoc MP).
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bioRxiv - Bioengineering 2022Quote: ... the cells were blocked with Bovine Serum Albumin (BSA) in DPBS for 30 min and then incubated with anti-His6 antibody (1:100, Bio-Rad, MCA1396) for 2 h at 25°C ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
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bioRxiv - Immunology 2022Quote: ... To each well 50 µL of 2 µg/mL of biotin-conjugated mouse anti-bovine IFN-γ mAb (CC302, Bio-Rad Antibodies) diluted in PBS was added and plates incubated for 2 hours at RT ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Plant Biology 2022Quote: ... Blots were incubated with α-TPLATE appendage antibodies (rabbit) (Dejonghe et al, 2019) and imaged on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Complete antibody conjugation was validated by running both conjugated and un-conjugated paired antibodies on a 4-15% SDS-PAGE gel (Bio-Rad #4561083) under non-reducing conditions ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were detected using chemiluminescence using horseradish peroxidase–conjugated secondary antibodies of anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) origin and enhanced chemiluminescence substrate (Thermo Scientific # 32106) ...
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bioRxiv - Physiology 2023Quote: ... The blots were washed four times with 0.1% tween TBS before being incubated for 1 h at room temperature with goat Anti-rabbit IgG HRP conjugated secondary antibody (Bio-Rad, USA) in 0.1% tween TBS buffer containing 3% BSA (Bovogen ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained in different cycles overnight at 4°C with the following antibodies: anti-CD3 (clone CD3-12; #MCA1477, Bio-Rad, USA), anti-CD206 (clone E6T5J ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (Cytiva) and images were acquired on ChemiDoc Touch (Bio-Rad Laboratories). The acquired images were processed and quantified using Image Lab software (Bio-Rad laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Each membrane was then incubated with the appropriate HRP-conjugated secondary antibodies in TBS-T and visualized by using the Enhanced chemiluminescence substrate (BioRad 170-5060) and ChemiDoc (BioRad).
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody incubation was done at room temperature for 1h (HRP-conjugated goat anti-rabbit IgG, 1:2000 dilution, Bio-Rad, 403005). Membranes were washed with Tris-buffered saline containing 0.05% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed as previously mentioned and the antigen-antibody complex was revealed by Clarity Enhanced Chemiluminescence Blotting Substrate (Bio-Rad, #1705060) using the iBright™ FL100 Imaging System (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were detected using chemiluminescence using HRP conjugated secondary antibodies of either anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) or anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Immunology 2023Quote: ... Blots were probed using primary antibodies in PBST (1X DPBS with 0.05% Tween-20 (BioXtra, catalog #P7949) with 5% Blotting Grade Blocker (Bio-Rad, catalog #1706404) for approximately 16 hours (overnight ...
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bioRxiv - Microbiology 2023Quote: ... The horse-radish peroxidase conjugated to the secondary antibody was detected by incubating the membrane with 2 mL chemiluminescence substrate (Bio-Rad, 1705062) for five minutes and then imaged using a ChemiDoc XRS+ imager (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The blocking buffer was removed and 10 ml 5% BSA-PBST with 1 μl RABBIT anti-DYKDDDDK Tag antibody (Bio-Rad, # AHP1074), 1 μl anti-MBP Monoclonal Antibody (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with secondary antibodies for 1 h at room temperature: goat anti-rabbit IgG (H+L)-HRP conjugate (170-6510, Biorad, 1:10000) and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 5% BSA for 60min at RT and incubated with the primary antibody (anti-NP, Bio-Rad, 1/5000) for 45-60min shaking ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies and later analyzed using a ChemiDocTM Imaging System (Bio-Rad, California, USA).
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used for Western blotting were goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Molecular Biology 2024Quote: ... CD45-APC and Bu1-PerCP-Cy5.5 were generated by conjugation of the antibody with the respective Lynx Rapid conjugation kit (Bio-Rad, Feldkirchen, Germany). Samples were analyzed on a FACS Canto II and FlowJo 10.9 software (BD ...
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bioRxiv - Immunology 2024Quote: ... Cells were then resuspended in RPMI complete media at 1×106 cells/ml and stimulated with 5 µg/ml of anti-Igκ plus anti-Igλ F(ab’)2 antibodies (BioRad, Table 2) for the indicated time at 37°C ...
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bioRxiv - Genetics 2024Quote: ... membranes were washed three times with PBST and incubated with the appropriate StarBright Blue Fluorescent secondary antibodies (Bio-Rad 12005866 or 12004161) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were incubated with primary antibodies 1:1000 rabbit anti-Iba1 (FujiFilm, 019-19741) and 1:300 rat anti-Cd68 (Bio-Rad, MCA1975) at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: Serum SARS2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then washed three times in TBST and probed with horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse antibody (Biorad, Feldkirchen, Germany). Vizualisation was done using enhanced chemiluminescence reagent (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Signals are captured following antibody’s instructions using a ChemiDoc MP Imaging system with Image Lab Touch Software (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The blot was then incubated with HRP-conjugated anti-His monoclonal antibody (1:5000) and detected with supersignalfemto substrate (Thermoscientific, USA) using ChemiDoc imaging system (Bio-Rad laboratories, USA).
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bioRxiv - Genetics 2021Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 hours at room temperature with HRP-conjugated rabbit secondary antibody (Bio-Rad, USA, 170-6515; RRID: AB_11125142) for NALCN detection and HRP-conjugated mouse secondary antibody (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are listed in Supplemental table 2 Immunoreactive proteins were visualized with Clarity Western ECL Substrate (Bio-Rad Laboratories, Hercules, CA, USA). The UVP ChemStudio (Analytik Jena US LLC ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... MNV or HAV RNA recovered by the ISO 15216-1 and PGM-MB or HAV antibody methods was quantified using the One-Step dd-RT-PCR Advanced Kit for Probes (Bio-Rad Laboratories, Ltd.) according to manufacturer’s instructions and as described previously (23 ...
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bioRxiv - Cell Biology 2020Quote: ... Appropriate secondary antibodies coupled to horseradish peroxidase were detected by enhanced chemiluminescence using ClarityTM Western ECL Blotting Substrate (Bio-Rad, Hercules, CA, USA). Resulting protein bands were quantified and normalised to each lane’s loading control using the ImageStudio software (LI-COR).