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Citations for Bio-Rad :
1651 - 1700 of 5223 citations for 6H Purin 6 one 1 2 3 9 tetrahydro 3 methyl 2 thioxo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Serum samples were obtained from blood collected from the submandibular vein at the indicated timepoints and incubated on Kallestad HEp-2 slides (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were prepared for loading by mixing 30 μg of protein in 5xSDS sample buffer containing 15% 2-Mercaptoethanol (Bio-Rad) and heated at 90 °C for 5 minutes ...
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bioRxiv - Physiology 2022Quote: ... followed by a final melting curve analysis from 70°C to 95°C with 0.2°C increment for each step using the CFX96 Real-Time PCR System and the Precision Melt Analysis software (Bio-Rad). For HMA analysis ...
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bioRxiv - Genomics 2024Quote: ... Samples were then run on a 2% agarose gel with 1x SYBR Safe for 2 hours at 120 V before gel extraction with a Freeze ‘N Squeeze column (Bio-Rad). After extraction ...
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bioRxiv - Genomics 2024Quote: ... 10ul of PCR product of each replicate for each gene was loaded in separate wells in 2% agarose gels and imaged using a ChemiDocTM MP Imaging system (BioRad, USA). All replicates for each gene were loaded on the same gel ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% of total input and 15% of total IP fraction were resolved on 10% SDS-PAGE gels and wet transferred onto a 0.45 µm nitrocellulose membrane for 2 h at 80 V and 4°C (Bio-Rad, Germany). The membrane was blocked for 1 h in 5% BSA in PBST (PBS + 0.2% Tween20) ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR was performed with the 2× S6 SYBR Premix EsTaq plus (Scintol) on the CFX Connect Real-Time System (Bio-Rad). The data were analysed using the 2-ΔΔCt method ...
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bioRxiv - Neuroscience 2023Quote: ... and the supernatants were incubated with either 2 µg of anti-PAR antibody (clone #19, custom designed at AbD Serotec, Bio-Rad) or Human IgG F(ab)2 (Rockland ...
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bioRxiv - Genomics 2024Quote: ... This cDNA was then run on a 2% agarose gel in TAE buffer alongside a 100bp-1kb DNA ladder (Bio-Rad). After electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 µg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then transferred to a PVDF membrane at 400mA for 2 hours at 4°C in 1x Tris/Glycine buffer (1610771, Bio-Rad). Membranes were then blocked in casein blocking buffer (PI37528 ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... before solubilisation in 2% SDS (Severn Biotech, Worcs, UK) and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad). Data are expressed as OD595 of selected timepoint relative to OD595 at day 0.
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bioRxiv - Cancer Biology 2023Quote: ... and the fold change was calculated using the 2−ΔΔCT method using Bio-Rad CFX384 Touch Real-Time PCR System and software (Bio-Rad). The results were expressed either as the relative log2 FC (fold change ...
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bioRxiv - Biophysics 2023Quote: ... The subsequent mixture was gently rotated at RT for 20 min prior to the addition of 250 mg SM-2 Bio-Beads (Bio-Rad) per 5 ml of sample ...
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bioRxiv - Cell Biology 2023Quote: ... The total cDNA obtained was diluted 1:10 and 2 µl was used for qRT-PCR by using gene specific primer and SYBR green PCR mix (BIO-RAD) on BIO-RAD CFX96 real time PCR machine ...
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bioRxiv - Immunology 2023Quote: ... Labeled glycans were dissolved in HPLC grade water (FisherScientific) and excess unbound APTS removed using Agencourt CleanSEQ beads (non-antigen specific glycans) and Bio-Gel P-2 (Bio-rad) size exclusion resin (RBD-specific glycans) ...
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bioRxiv - Neuroscience 2023Quote: ... F0 fish were screened by high resolution melt (HRM) analysis using gene-specific primers (Table 2) with Precision melt supermix (Biorad 1725112) according to the manufacturer’s protocol in a BioRad CFX RT-PCR thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR products were run on a 2% agarose gel and images were taken on a Chemidoc MP imaging system (BIO-RAD). Image J was used to analyze the pixel intensity of 3R (288 bp ...
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bioRxiv - Biochemistry 2023Quote: ... for 2 h (for the ERGIC-53 ΔΗ34 mutant) The beads were transferred into a gravity flow column (Econo-column, Bio-Rad) and washed with 20 CV of buffer A supplemented with 0.02% (w/v ...
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bioRxiv - Immunology 2023Quote: ... was used for positive signals and the chemiluminescence was detected using a Light Capture AE-6971/2 device (ATTO) or ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... before being re-incubated with the secondary antibodies at room temperatures for 2 h and then subjected to the imaging of immunoblots by Bio-Rad instrument ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Genomics 2023Quote: ... 10ul of PCR product of each replicate for each gene was loaded in separate wells in 2% agarose gels and imaged using a ChemiDocTM MP Imaging system (BioRad, USA). All replicates for each gene were loaded on the same gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of RNA template was converted to cDNA using iScript™ cDNA Synthesis Kit (1708891, Bio-Rad, Hercules, CA, USA), according to the manufactures’ instructions ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription reaction was conducted using 0.5 µg of total RNA and 2 µl of iScript™ Reverse Transcription Supermix (Bio-Rad) in 10 µl reaction volume ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747, BioRad, Hercules, CA, USA) and run on a Mini-PROTEAN® TGX™ Precast Gel (#4561083 ...
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bioRxiv - Cancer Biology 2023Quote: ... before solubilisation in 2% SDS (Severn Biotech, Worcs, UK) and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad). Data were normalised by dividing raw OCR/ECAR measurement by OD595 for each well of the culture plate ...
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bioRxiv - Biochemistry 2023Quote: ... This mixture was then incubated at 4°C for 2 h with gentle agitation before reconstitution was initiated by removing detergent by incubating with Bio-beads (Bio-Rad) at 4°C overnight with constant rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction products were visualized on a 2 % agarose gel with 0.05% (v/v) Ethidium Bromide and visualized with a Gel Doc XR+ imager (Bio-Rad Laboratories). PCR amplicons were column purified with the Monarch® PCR & DNA Cleanup Kit and eluted in 25 µL of Monarch® DNA Elution Buffer.
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bioRxiv - Biophysics 2024Quote: ... then diluted below the CMC of C12E8 with reconstitution buffer and added to 0.5 g methanol-activated Biobeads SM-2 (Bio-Rad; 1523920). The mixture was rotated end over end for 18 hours and then subject to room temperature size exclusion chromatography on a Superose 6 10/300 GL column ...
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bioRxiv - Genetics 2024Quote: ... Membranes were were in contact with the luminol-H2O2 solution for 2 minutes and then exposed for imaging using the Chemidoc imaging system (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... clarified at 20,000 g for 2 minutes and 15 µL was loaded onto a 4-20% tris-glycine SDS-PAGE gel (Biorad #5671094). Proteins were then transferred to nitrocellulose (Sigma #10600001 ...
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bioRxiv - Microbiology 2024Quote: ... 10 µg Capped mRNA transcripts were electroporated into BHK-21 cells in a 0.2 cm cuvette (25 μF, 1.5 kV, and 200 Ω) with the help of Gene Pulser Xcell Electroporation system (Bio-Rad) and resuspended in antibiotic-free DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... pH6.9 using a micro biospin 6 column (Bio-Rad). The FOS sample was diluted to ∼500 µM with 0.5 M ammonium acetate ...
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bioRxiv - Microbiology 2020Quote: ... and Bio-Plex Manager software (v.6, Bio-Rad). The data were expressed as median fluorescence intensity (MFI ...
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bioRxiv - Neuroscience 2024Quote: ... 6-8 mg of 1.6 µm gold beads (BioRad) were coated with 15 µg of EGFP-N1 (Clontech) ...
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bioRxiv - Molecular Biology 2023Quote: To prepare the tubing for gene gun delivery one tubing and 25 mg of Au particles with 1 µm Ø was used from Helios Gene Gun Optimization Kit (Bio-Rad, Hercules, CA, USA). 10 µg of the synthesized RNA transcript was used four labeling Au particles ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
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bioRxiv - Immunology 2024Quote: ... were covalently coupled to carboxyl-functionalized fluorescent magnetic beads (1-3µg/1.25*10^6 beads) (Luminex Corp. MagPlex® Microspheres; Bio-Rad; Temse, Belgium) employing the BioPlex amine coupling kit (Ref. ...
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bioRxiv - Bioengineering 2020Quote: ... EG depletion was monitored using on an Aminex HPX-87H ion exclusion column (300 mm x 7.8 mm, particle size 9 μm; Bio-rad). The column was maintained at 40°C and samples were isocratically eluted using 0.014 N H2SO4 at a flow rate of 0.55 ml min-1 and read on a refractive index detector (RID) ...
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bioRxiv - Immunology 2022Quote: ... Lysates containing 40 μg of proteins were analyzed by SDS-PAGE on 9-13% gels and proteins were transferred onto nitrocellulose membranes (BioRad). The blots were blocked in non-fat milk diluted in tris-buffered saline (TBS ...