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Citations for Bio-Rad :
1551 - 1600 of 6633 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The blot was visualized for hACE2 band using chemiluminescence substrate in Chem-Doc imaging system (Bio-Rad). For internal control ...
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bioRxiv - Developmental Biology 2022Quote: ... the signals were detected using WESTARNOVA2.0 chemiluminescent substrate (Cyanagen) and the ChemiDoc MP Imaging system (Bio-Rad). For western blots after Co-IP SuperSignalTM West Femto Maximum Sensitivity Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes probed with HRP conjugated antibodies were developed using Clarity™ Western ECL Substrate (BioRad, 170-5061). Membranes were imaged using BioRad ChemiDoc™ MP Imaging System.
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed 3X with 0.1% TBS-T followed by incubation with Clarity Western ECL Substrate (BioRad). Chemiluminescence signal was detected using UVP ChemStudio (Analytic Jena).
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bioRxiv - Neuroscience 2023Quote: ... we used horseradish peroxidase-conjugated secondary antibodies (Cell Signalling) and Clarity Western ECL substrate (1705060, Bio-Rad) or SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cell Biology 2023Quote: Transfer was performed using iBlot2 (Thermo) and immunoblots were developed using Clarity Western ECL substrate (Bio-Rad) for HRP-linked secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... and detected using the Clarity Western enhanced-chemiluminescence (ECL) substrate as describes by the manufacturer (Bio-Rad). Images were acquired and analyzed with the ImageQuant Las 4000 luminescent image analyzer.
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bioRxiv - Microbiology 2024Quote: ... then washed with TBS-T and detected by chemiluminescent substrate using a Chemidoc MP imaging system (BioRad).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The chemiluminescence signals were obtained with Clarity Western ECL Substrate (Bio-Rad, California, USA, Cat. No. 1705060) and captured with ChemiDoc XRS+ system (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Primary antibodies were labeled with horseradish peroxidase-conjugated secondary antibody (1:5000) and ECL substrates (Bio-Rad). The densitometric intensities of the bands were quantified by using ImageJ software.
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bioRxiv - Immunology 2024Quote: ... Signal was detected by chemoluminescence (Fusion FX imaging system, Vilber) after exposition to Clarity ECL substrate (Biorad).
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bioRxiv - Neuroscience 2024Quote: ... The peroxidase reaction for AffiniPure antibodies was developed with Clarity™ Western ECL substrate (Bio-Rad, 1705061). Imaging was performed using either an Odyssey Fc imaging system (Li-Cor ...
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bioRxiv - Immunology 2023Quote: ... Western blots were revealed with BioRad Clarity Max western ECL substrate and imaged using ChemiDoc MP (BioRad).
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bioRxiv - Molecular Biology 2024Quote: ... The blot was developed as per instructions for chemiluminescence using Clarity Western ECL Substrate (1705061; Bio-Rad). The relative density was measured using Image J software.
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... 30 second at 72°C and a final extension of 7 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). Five μl of PCR product was visualized on a non-denaturing 1% agarose gel with a 100bp ladder ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 seconds at 72°C and a final extension of 5 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). In order to standardize the expression pattern of NvDsx ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Biophysics 2019Quote: ... Temperature was increased at a rate of 1°C per minute from 4°C to 100°C and fluorescence was measured every minute with a qPCR thermocycler in FRET mode (Bio-Rad). Aggregation temperature was determined by locating the global minimum of the second derivative of the raw data ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated at a rate of 0.5 °C per minute from 25 °C to 95 °C and fluorescence signals were recorded with the CFX Connect Real-Time PCR Detection System (Bio-Rad). The melting temperatures were analyzed using the derivative plots of the melting curve.
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bioRxiv - Microbiology 2020Quote: ... was added to each well and samples were heated from 25 to 95 °C in 1 °C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation/emission filters of 492 and 516 nm were used to monitor the fluorescence increase resulting from binding of the Sypro Orange to exposed hydrophobic regions of the unfolding MsSepFcore ...
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bioRxiv - Plant Biology 2021Quote: ... All qPCR reactions were run for 94°C for 2 min followed by 94°C for 15 s and 60°C for 1 min in a BioRad CFX384 Real Time System (Biorad, USA) qPCR machine ...
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bioRxiv - Microbiology 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative −d(RFU)/dT was determined to be the Tm.
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bioRxiv - Cancer Biology 2022Quote: ... then 40 cycles of 15 seconds at 95°C and 60 seconds at 60°C using CFX96 Touch Real-Time PCR Detection System (Bio-rad). A standard curve was derived from the serial dilutions of DNA template following concentrations ...
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bioRxiv - Biochemistry 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative ...
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Bioengineering 2020Quote: ... followed by 40 cycles of 95°C for 5 s and 60°C for 30 s (Bio-Rad CFX manager 3.1). Quantification of mRNA levels of different genes was performed using the 2−ΔΔct method ...
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bioRxiv - Microbiology 2021Quote: ... 60°C for 30 sec and 72°C for 30 sec on a Bio-Rad CFX96 qPCR cycler (Bio-Rad, Germany).
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bioRxiv - Microbiology 2022Quote: ... and 40 cycles of PCR (5 s at 95°C followed by 20 s at 60°C) in a CFX Opus 96 Real-Time PCR System (Bio-Rad). Results were analyzed by the Comparative CT Method (ΔΔCt Method) ...
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bioRxiv - Biochemistry 2022Quote: ... was measured over temperatures ranging from 10 °C to 95 °C using a CFX Connect Real-Time PCR Detection System (Bio-Rad). Melting temperature (Tm ...
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bioRxiv - Neuroscience 2022Quote: ... at either 95°C for 5 minutes or at 70°C for 10 minutes and resolved in a 4-15% Precast Gels (Bio-Rad, 12-well ...
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bioRxiv - Microbiology 2023Quote: ... was added to each well and the mixture was heated from 25 to 95°C in 1°C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation and emission filters of 492 and 516 nm respectively ...
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bioRxiv - Neuroscience 2023Quote: ... with a melt-curve from 65 to 95 °C (in 0.5 °C increments per 5 s) using CFX384 Touch Real-Time PCR Detection System (Bio-Rad, 1845384). Quantification of mRNA was determined using the comparative cycles to threshold CT (ΔΔCT ...
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bioRxiv - Immunology 2023Quote: ... extension at 72°C for 30sec and final extension at 72°C for 5minutes) were conducted in Thermocycler (Bio-Rad, USA). Positive control samples were taken from Molecular Parasitology Laboratory ...
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bioRxiv - Physiology 2023Quote: ... Lysates were then incubated for 5 min at 37 °C and 5 min at 75 °C in a thermocycler (T100, Bio-Rad), immediately followed by storage at -80 °C until further processing.
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Immunology 2021Quote: ... TMB substrate was added to stop the reaction before performing the reading at 450 nm in the ELISA plate reader (iMark Microplate Reader, Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... Germany) and lysosomes with rat monoclonal anti-Cd68 (clone FA-11, #MCA1957, Bio-Rad, Hercules, CA, USA). In the second set ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Neuroscience 2022Quote: ... was used for quantitative real time PCR with commercially available rat Magel2 and Gapdh primers (Bio-Rad, USA ...
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bioRxiv - Neuroscience 2024Quote: ... For analysis the Bio-Flex Pro™ Rat Cytokines Assay (Bio-Rad, 24-plex panel #171-K1001M) was used on a Bio-Plex® MAGPRIX™ multiplex reader and analysed with Bio-Plex Analysis software (Bio-Rad).
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bioRxiv - Plant Biology 2019Quote: ... Membranes were developed with 50/50 mix of ‘pico’ and ‘femto’ chemiluminescence substrates (Thermo) on a ChemiDoc (Biorad).
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bioRxiv - Biochemistry 2021Quote: ... Immunoblots were visualized using horseradish peroxidase–tagged secondary antibody and chemiluminescence (Clarity Western ECL Substrate; BioRad, Hercules, CA). The antibodies used included those directed to lamin A/C ...
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bioRxiv - Plant Biology 2021Quote: ... After washing 3 times with TBST the chemiluminescent was conducted by adding Clarity Western ECL Substrate (Biorad, #1705061) for 2 min and detected by ChemiDoc imaging system (Biorad) ...
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bioRxiv - Microbiology 2021Quote: ... Blots were developed using the chemiluminescent HRP substrate and scanned by the Image Lab software (Bio-Rad, USA). The relative differences in expression patterns of selected cytokines among the different groups of samples were assessed using the GraphPadPrism8 software.
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bioRxiv - Synthetic Biology 2022Quote: ... Membranes were washed an additional four times in TBST buffer before Clarity Max Western ECL Substrate (Bio-rad) was applied and the membrane was imaged for chemiluminescence ...
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bioRxiv - Biochemistry 2022Quote: ... Protein quantity was extrapolated based on HRP activity using the Clarity™ Western ECL Substrate (Bio-Rad Laboratories), measured on a ChemiDoc XRS+ (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... The Amersham™ Imager 600 was used to detect HRP-conjugated secondary antibodies using a chemiluminescent substrate (Biorad). Densitometry analysis was performed using ImageJ software.
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bioRxiv - Microbiology 2022Quote: ... Chemiluminescence was induced using the SuperSignal West Pico substrate (Pierce) and imaged on a ChemiDoc XRS+ (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Blots were developed with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo) and imaged on a Chemidoc XRS+ (BioRad). All assays were performed in triplicate and immunoblot quantification was done with ImageJ ...