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Citations for Bio-Rad :
1451 - 1500 of 6633 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the blot was washed thrice with 0.1% TBST and developed using Clarity Western ECL substrate (Biorad) on a GEImageQuant LAS 4000 system ...
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bioRxiv - Molecular Biology 2023Quote: ... The immunoreactive bands were visualized by a Clarity Max™ Western ECL substrate (Bio-Rad #1705062) according to the manufacturer’s protocol and an iBright Imaging systems (Thermo Fisher Scientific #CL1000).
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bioRxiv - Microbiology 2023Quote: ... for an hour at RT and imaged with the Clarify Western ECL substrate solutions (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Detection was performed according to the manufacturer’s instructions for the ECL blotting substrate (Bio-Rad, 1705061) and visualized using the ChemiDoc™ XRS+ System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were then washed three times with TBST and developed using Clarity Western ECL Substrate (BioRad) on a ChemiDoc imaging system (BioRad ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was detected using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Biophysics 2023Quote: ... were developed with SuperSignal West Pico Chemiluminescent Substrate (ThermoScientific, 34077) using a Chemidoc scanner (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were developed for ECL detection (Clarity Western ECL substrate, Bio-Rad Laboratories, Hercules, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Clarity Max Western ECL substrate was added to the membranes for 5 mins (Bio-Rad #1705062) and chemiluminescent signal imaged using a Chemidoc imager (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... after incubating each membrane with the enhanced chemo-luminescent (ECL) Clarity Western ECL substrates (1705060, BioRad) for 1-2 minutes at RT ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated secondary antibodies were visualized using Clarity™ Western ECL Substrate (Cat # 1705061, Bio-Rad) and blot images captured using the ChemiDoc™ MP Imaging System (Cat # 12003154 Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Microbiology 2023Quote: ... were used with Clarity Western ECL Substrate on the ChemiDoc Touch Imaging Sysytem (Bio-Rad Laboratories). For analyses of protein band intensities ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were HRP-conjugated and imaging was performed using Clarity Western ECL substrate (Bio-rad) and a Chemidoc imaging system (Bio-rad)
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bioRxiv - Biochemistry 2024Quote: ... Luminescence detection was done using a Clarity Western ECL substrate (1705060, Bio-Rad Laboratories, Hercules, CA) in a ChemiDoc Touch imaging system (version 3.1 ...
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bioRxiv - Microbiology 2024Quote: ... An HRP-conjugated goat anti-mouse secondary antibody and the clarity Western ECL substrate (Biorad, 1705060) were used for protein detection ...
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bioRxiv - Bioengineering 2023Quote: ... and Clarity Max Western Enhanced Chemiluminescence (ECL) Substrate (1705060) were purchased from Bio-Rad (CA, USA). RIPA Buffer (9806) ...
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bioRxiv - Immunology 2020Quote: ... IL-23 was measured using either a mouse IL-23 ELISA (R&D) or Luminex based method from BioRad. IL-12p40 ...
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bioRxiv - Immunology 2020Quote: ... followed by measuring the absorbance of individual wells at 450 nm using an ELISA reader (Bio-rad mod.550). Serum samples from unmanipualted mice were used for negative controls.
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bioRxiv - Biochemistry 2020Quote: ... The temperature was varied 25 - 95 °C in 0.5 °C increments by using an iCycle IQ5 real-time PCR system (BioRad). Relative fluorescence was measured using excitation and emission wavelengths of 580 nm and 623 nm ...
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bioRxiv - Molecular Biology 2019Quote: ... primer and probe hybridization temperature that was checked individually by PCR using a gradient ranging from 57.5 to 61.4°C in intervals of 0.8°C (CFX96 Touch™ Bio-Rad), ii ...
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bioRxiv - Biochemistry 2021Quote: ... MDH2 aliquots were heated to 42 °C for 10 min then returned to 37 °C in a thermocycler (BioRad), while native samples were maintained at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence was measured at 512 nm along a temperature gradient from 20 to 100 °C (0.5°C increase every 10 s) in the CFX ConnectTM real-time thermocycler (BioRad) with the Cfx MaestroTM software ...
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bioRxiv - Biochemistry 2019Quote: ... The microsomal fraction was aliquoted and stored at −80 °C after protein quantification with the Quick Start Bradford Protein Assay Kit (Bio-Rad Laboratories, Munich, Germany).
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bioRxiv - Neuroscience 2023Quote: ... frozen in liquid nitrogen and stored at −80°C until the RNA isolation with Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA, USA), as described (Makarava et al. ...
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bioRxiv - Physiology 2019Quote: ... initiation at 95°C/10 s followed by 45 amplification cycles (60°C/15 s) on the CFX Thermal cycler (Biorad).
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bioRxiv - Systems Biology 2021Quote: ... followed by 15 s at 95 °C and 60 s at 58 °C for 40 cycles using Biorad CFX384 thermocycler (Biorad). The mRNA levels of genes encoding cytokine expression were normalized relative to the mean levels of the housekeeping gene and compared using the 2−ΔΔCt method as described previously2 ...
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bioRxiv - Systems Biology 2020Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Systems Biology 2020Quote: ... 40 cycles of PCR denaturing for 5 secs at 95 °C and annealing/extending for 30 secs at 55 °C) in real-time PCR system (CFX384 Touch, Biorad).
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bioRxiv - Microbiology 2020Quote: ... 73105b and 73106f pseudotyped viruses were incubated at temperatures from 37°C to 50°C for 60 minutes using temperature gradient on PCR thermal cycler (BioRad). Pseudoviruses were then aliquoted in a 96-well culture plate ...
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bioRxiv - Biophysics 2022Quote: ... The protein sample was incubated at 40°C and 80 °C respectively for 1 h in a PCR cycler (BioRad) with a heated lid to prevent evaporation ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR was carried out in triplicates and was performed at a 2-step reaction with 95°C denaturation and 56°C annealing and extension for 35 cycles on a CFX96 Real-Time System (BIORAD). Relative quantification of target genes was determined using Bio-Rad CFX manager 3.1 software ...
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bioRxiv - Neuroscience 2022Quote: ... then denatured for 5 min at 95°C and annealed by gradual cooling down at −0.1 °C/sec using a PCR thermocycler (BioRad T100). Annealed oligos were subsequently inserted into BbsI-digested vector pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230) ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was then heated up to 95°C for 5 minutes and cooled down to 30°C at the rate of 1°C per minute using a thermal cycler (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... at a ratio of 1:2 in 100 µL of TE by heating to 95°C for 3 min followed by cooling to 25°C at 0.1 °C/min in a T100 thermal cycler (Bio-Rad). In Figures ...
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bioRxiv - Molecular Biology 2022Quote: ... and heating to 95°C for 3 min followed by cooling to 25°C at 0.1 °C/min in a T100 thermal cycler (Bio-Rad). Primer extension assays were performed either as time courses (up to 240 min ...
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bioRxiv - Zoology 2022Quote: ... and folded by heating to 95°C for 2 min and slowly cooling down at 0.1 °C per second using a thermocycler (Bio-Rad). DsRNA concentrations were adjusted to 3 or 14 μg / μl using SpeedVac Concentrator (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared assay plate was exposed to temperature increases from 25 ⁰C to 95 ⁰C with 1 ⁰C increments for 5 s each using a CFX Connect Real-Time PCR Detection System (BioRad). SYBR fluorescence was detected after every temperature increase ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 40 cycles of 95 °C for 5 sec and 60 °C for 30 sec using the CFX 96 real-time systems (Biorad). The 2−ΔΔCt method was used to analyze the data (Schmittgen and Livak ...
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bioRxiv - Systems Biology 2023Quote: ... 40 cycles of PCR denaturing for 5 secs at 95 °C and annealing/extending for 30 secs at 55 °C) in real-time PCR system (CFX384 Touch, Biorad). The results were normalized to GAPDH from the same well ...
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bioRxiv - Cancer Biology 2022Quote: ... 95°C for 2 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min (BioRad). The following primers from Integrated DNA Technologies (IDT ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated from 10 °C to 95 °C (10 s at each 0.5 °C step) using a CFX Connect qPCR with CFX Manager software (Bio-Rad). Melting temperatures were calculated with DSFWorld27 (by model 2).
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bioRxiv - Microbiology 2023Quote: ... and 40 cycles of PCR (5 s at 95°C followed by 20 s at 60°C) in a CFX Opus 96 Real-Time PCR System (BioRad). Results were analyzed by the Comparative Ct Method (ΔΔCt Method ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 15 minutes at 50°C and 5 minutes at 85°C in T100 Thermal Cycler (1861096, Bio-Rad). Real-time qPCR reactions were performed using SsoAdvanced Universal SYBR® Green Supermix (1725274 ...
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bioRxiv - Systems Biology 2023Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). For gene expression assays ...
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bioRxiv - Plant Biology 2024Quote: ... using 45 cycles of 15 sec at 95°C and 30 sec at 60°C in a CFX96 cycler (Biorad). Data were normalized to the transcript encoding PP2A subunit A3 (At1g13320 ...
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bioRxiv - Neuroscience 2024Quote: ... 72°C (30s) and 1 cycle of 72°C for 5 min (C1000 Touch Thermal Cycler, Bio-Rad, Hercules, CA). 2–5 μl of the PCR reactions were subjected to gel electrophoresis using a 3% agarose gel in tris-borate-ethylenediamine tetraacetic acid buffer ...
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bioRxiv - Plant Biology 2024Quote: ... using 45 cycles of 15 s at 95°C and 30 s at 60°C in a CFX96 cycler (Biorad). Primers are listed in Additional file 1.
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bioRxiv - Immunology 2019Quote: ... and a primary rat anti-mouse CD68 antibody (activated microglia/macrophages, 1:200 dilution, MCA1957, Biorad) with secondary goat anti-rat Alexa-Fluor 555 (1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: anti–α-tubulin (MCA77G YL1/2, rat monoclonal; Bio-Rad), anti-GAPDH (mouse ...