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Citations for Bio-Rad :
1501 - 1550 of 8354 citations for 7 8 DIHYDRO 1 3 DIOXOLO 4 5 G ISOQUINOLIN 5 6H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 50 seconds at 72°C and a final extension of 5 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). In order to standardize the expression pattern of NvDsx ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were then diluted 1:10 and 2 µL of diluted cDNA were used for RT-qPCR along with 5 µL of iTaq universal SYBR mix (Bio-Rad), 1.5 µL of primers (1.5 µM mix of forward and reverse ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... Up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Microbiology 2019Quote: T1L virions (2×1012 particles/ml) were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of the reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed 3 times for 5 min with TBST after secondary antibody incubation and chemiluminescence signal was detected using Clarity Western ECL Substrate (Bio-Rad). All relevant information on antibodies used in this study are listed in Table S2.
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bioRxiv - Bioengineering 2021Quote: ... This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad) for 1 h with rocking ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction components were prepared as a 2x-master mix and 5 µl of the mix was pipetted into opaque wells of 384-well PCR-plate (Bio-Rad). Then an equal volume of sample was added ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 min and proteins were resolved on 10% SDS polyacrylamide gels and blotted onto nitrocellulose membranes (Bio-Rad). Anti-rabbit Phospho-p44/42 MAPK antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Assays comprising of premixtures of a forward and reverse primer (18 μM each) with a FAM- or HEX-conjugated hydrolysis probe (5 μM) were either purchased from Bio-Rad, based on previous publications (Roberts et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Gene expression analysis were performed by quantitative RT-qPCR in a Viia7PCR device with 5 μl reaction mix containing SyberGreen Supermix (Bio-Rad). Tomato actin was used as a reference gene and fold change in expression were calculated based on 2^(-ddCt ...
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bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... The total PCR reaction volume was 15 µL per well (10 µL master mix + 5 µL cDNA sample) and the qPCR was performed on the CFX384 Touch Real-Time PCR Detection System (Bio-Rad) using the cycling conditions listed below:
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed again for 3 × 5-min in TBS-T before being immersed for 5 min under gentle agitation at room temperature in Clarity ECL detection substrate (Bio-rad Laboratories Pty Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... Standard qPCR reactions with 5 or 10 ng cDNA per reaction were performed with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX Connect Real-Time PCR Detection System ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were run at 90 V for 120 min and there-after stained with Ethidium bromide at room temperature for 5 min and visualised using a Gel Doc XR imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... for 30 minutes at room temperature was followed by three 5 minute washes in milliQ water before imaging on a ChemiDoc MP (Bio-Rad).
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bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude) ...
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bioRxiv - Cell Biology 2022Quote: ... denatured in 2x Laemmli buffer (5 min at +95°C) and 20 µg were loaded per well of Mini-PROTEAN Precast Gel (Bio-Rad). The protein transfer was done with the Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... The stoichiometry of condensin II subunits for each preparation was checked by 5% SDS-PAGE followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with beta-mercaptoethanol and denaturated at 95 °C for 5 min before loading on a 12 % PAA precast gel (#4561044, Bio-Rad). The gel was run at 70 V for 15 min and then 100 V for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with appropriate primary and secondary antibodies listed in Table 2 in 5% milk and developed using the Clarity Western enhanced chemiluminescence substrate system (1705060, Bio-Rad) in a Xograph Compact X5 processor ...
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bioRxiv - Microbiology 2020Quote: Total protein extracts (5 × 106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) [55] ...
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bioRxiv - Developmental Biology 2020Quote: ... Total protein lysates were prepared from 25 pair of testes and loaded in equal dilution series (5, 10, and 20 μg) onto precast stain-free PAAG gel (Bio-Rad). After electrophoresis ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25% of beads by heating at 95°C for 5 minutes in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from the remaining 75% of beads using TRIzol (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were boiled for 5 min at 95°C in 1x XT sample buffer containing 1x XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT precast gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were denatured for 5 min at 95 °C and run on Mini or Midi TGX-Stain-free precast gels (Bio-rad) at 110V for 60 min (mini gels ...
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bioRxiv - Immunology 2020Quote: ... polβ and Exo1 to the 5’Sμ (in CH12-F3) was assessed by quantifying ChIP-enriched DNA using CFX384 realtime PCR (Bio-Rad). The following primers were used for qPCR:
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed four times in PBST for 5 minutes at room temperature and imaged directly using a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Biophysics 2019Quote: ... Gradients were manually fractionated into 200 μL fractions and protein concentrations were determined by the Bradford assay (Protein Assay Dye Reagent Concentrate 5×, Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... 14 µL of PCR product were then transformed by electroporation in 90 µL cells (2.5 kV electric pulse for 5 ms, Bio-Rad micropulser). Cells were recovered in SOC outgrowth medium (NEB B9020S ...
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bioRxiv - Immunology 2022Quote: ... Transfection took place over two rounds of electroporation at 850 V and 25 μF with an interval of 5 secs (Bio-Rad Gene Pulser Xcell Electroporation Systems) ...
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bioRxiv - Neuroscience 2019Quote: ... and then eluted by boiling in SDS lysis buffer for 5 minutes at 95°C and resolved by SDS-PAGE (Bio-rad) gel ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were boiled for 5 min in 1X XT sample buffer containing 1X XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT Precast 26-well gels (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... 80 μL of the supernatant was removed (termed ‘whole cell extract [WCE] sample’) and 5 μg of antibody (anti-PK (Bio-Rad) or anti-HA (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions or ISVPs (2×1012 particles/ml) were incubated for 5 min at the indicated temperatures in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative RT-PCR (qRT-PCR) reactions were performed in 10 μL volumes using 5 μL of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad), 4 μL of a mix containing 0.5 μM of each transcript-specific primer (Table S2) ...
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bioRxiv - Immunology 2019Quote: ... NK cells were pre-incubated with activating IB4 antibody (IgG2a, gift from Dr. Fabio Malavasi, University of Torino) blocking CD38 (clone AT13/5, Bio-Rad), CD31 (clone WM59 ...
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bioRxiv - Bioengineering 2019Quote: ... The fractions were analyzed by SDS-Page and fractions containing the protein of interest were pooled and loaded onto a 5 mL Mini-profinity IMAC column (Bio-Rad). Metal affinity chromatography was performed as previously described for the TEV protease ...
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bioRxiv - Microbiology 2019Quote: ... The gel was stained with 5 μg/ml ethidium bromide for 20 min and imaged using a Chemidoc XRS+ (Bio-Rad).
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bioRxiv - Microbiology 2019Quote: ... Reactions were run in triplicate in a Bio-Rad CFX96 Touch C1000 qPCR machine in 10 μl final volumes with 5 μl iTaq Universal SYBR Green Supermix (Bio-Rad), 0.5 μl of each primer and 1 μl DNA extract ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked 1 hr at room temperature with Odyssey PBS Blocking Buffer (Li-Cor, 927-40000) or in 5% Blotting-Grade Blocker (Biorad, 1706404) in TBST (50 mM Tris buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The blots were washed three times with TBS-T for 5 min each time before to development with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging on the ChemiDoc XRS+ System (Bio-Rad).
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... The incubation was terminated by boiling the samples at 95°C for 5 minutes in 1X Laemmli sample buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (rat (IgM)/LM20 and LM19 mouse (IgG)/2F4 (in 5% milk PBS) and after washing chemiluminescence induced with Clarity Western ECL Substrate (Biorad, #1705061) and detected with ChemiDoc imaging system (Biorad).
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bioRxiv - Synthetic Biology 2020Quote: ... The samples were run with 5 mM H2SO4 at 0.6 mL/min on an Aminex HPX-87H (Biorad Inc., CA, USA) column at 50°C ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed four times in PBST for 5 minutes at room temperature with agitation and imaged directly using a ChemiDoc MP imaging system (Bio-Rad). Alternatively ...