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Citations for Bio-Rad :
101 - 150 of 3617 citations for Sodium Voltage Gated Channel Alpha Subunit 10 SCN10A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... pH 9.0 running buffer and visualized using the FITC channel set at 100 µM resolution in low intensity mode on a GelDoc Pharos imager (BioRad Laboratories). ImageQuant software was used to analyse the fraction of probe bound in each lane and GraphPad Prism version 8 software was used to calculate the Kd ...
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bioRxiv - Biochemistry 2021Quote: ... The melting curves were measured at 1°C/min at the FRET channel in triplicate measurements and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Biochemistry 2020Quote: ... The melting curves were measured at 1°C/min at the FRET channel and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Biophysics 2022Quote: ... The real-time measurement of fluorescence using the HEX channel (excitation 515-535 nm, emission 560-580 nm) was performed in CFX Connect Thermal Cycler (Bio-Rad). Samples were heated from 25°C to 95°C at 0.2°C min-1 ...
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bioRxiv - Neuroscience 2021Quote: ... were added to the 8 channel droplet generation cartridge according to manufacturer instructions and this cartridge was placed in the QX200 droplet generator (Bio-Rad). Then ...
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bioRxiv - Microbiology 2023Quote: ... Amplification was quantified in real-time by detecting DNA-bound SYBR Green on the FAM channel of a CFX1000 instrument (Bio-Rad) and cycle thresholds (Cq ...
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bioRxiv - Microbiology 2023Quote: ... Each assembled ddPCR reaction mixture was then loaded into the sample well of an eight-channel disposable droplet generator cartridge (Bio-Rad). A volume of 70 μL of droplet generation oil (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the number of positive and negative droplets was quantified for both Fam and Hex channels using the QX200 droplet reader (Bio-Rad) and the positive/negative ratio was used to estimate the initial gDNA concentration of each gene assuming a Poisson distribution ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Expressions optical density were determined and presented as ratio against GAPDH with the help of alpha imager (Bio-Rad). All the expressions were checked in triplicate (Table 1).
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bioRxiv - Immunology 2020Quote: ... We used FITC-conjugated mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α (clone 76-2-11 ...
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bioRxiv - Neuroscience 2020Quote: ... The antibodies were purified by immunoaffinity chromatography using antigen-coupled affi-gel 10 (Bio-rad).
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (BioRad). Following gel electrophoresis ...
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bioRxiv - Microbiology 2022Quote: ... To address the limited number of channels in our commercial ddPCR system (e.g., QX200 Droplet Digital PCR system, Bio-Rad, Hercules, USA), we modulated the amplicon length and primer/probe concentration in the reaction mixture to enable the separation of different targets within the same color (Levy et al. ...
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bioRxiv - Biochemistry 2021Quote: ... surface CD8 was labeled with 10 µg ml−1 AlexaFluor647-anti-CD8 antibody (Bio-Rad, MCA1226A647) at 37 °C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used: goat α-mouse (1:10 000, Bio-Rad, 170-6515), donkey α-goat (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% (w/v) of sodium dodecyl sulfate (SDS, Biorad, catalog #: 1610302). Protein ladder was added (Biorad ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad). Protein was transferred from the gel to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sodium dodecyl sulfate (SDS) and PVDF membrane were procured from Bio-Rad. The protein ladder was purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The results were obtained from channels 1 (FAM) and 2 (HEX/VIC) and analysed with QuantaSoft™ Analysis Pro 1.0 (Bio-Rad Laboratories, Inc). All ddPCR reactions were prepared with QX200™ ddPCR™ EvaGreen® Supermix (2x).
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bioRxiv - Neuroscience 2020Quote: ... 20μL reactions were prepared in triplicate in 96-well white plates (Alpha Laboratories, UK) consisting of 10μL iTaq™ Universal SYBR® Green Supermix (BioRad), 1μL 20x TaqMan gene expression assay ...
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bioRxiv - Cell Biology 2022Quote: ... and imaged either with MultiImage Light Cabinet using FluorChem 8900 software (Alpha Innotech Corporation, San Leandro, CA, USA) or Gel Doc XR+ System with Image Lab Software (Bio-Rad). Coomassie staining with Colloidal Blue staining kit (Thermo Fisher Scientific Cat# LC6025 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies against CBX2 were affinity purified using the Affi-gel (Bio-Rad, Affi-Gel 10, Cat# 1536099)-coupled FLAG-CBX2-ΔCBox ...
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bioRxiv - Physiology 2022Quote: ... and antibodies were affinity purified from the sera using Affigel 10 beads (BioRad Laboratories, Hercules, CA, USA) loaded with the antigens according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... Colonies that were identified as expressing mCherry based on fluorescence at 670 nm (Cy5 channel) using a Chemidoc MP Imaging System (Bio-Rad, Mississauga, Ontario, Canada) were streaked onto M9 glucose plates supplemented with 50 μg/mL gentamycin and 100 μg/mL ampicillin until pure cultures were obtained.
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bioRxiv - Microbiology 2019Quote: ... and 1 mM sodium vanadate and equilibrated for protein content (Biorad assay kit). All lysates were resolved by SDS-PAGE electrophoresis and transferred to PVDF membranes ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were eluted into sodium dodecyl sulfate (SDS) sample buffer (Bio-Rad) containing 5% 2-mercaptoethanl (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... separated on a 4-15% sodium dodecyl sulfate/ polyacrylamide gel electrophoresis gel (BioRad) and transferred to a nitrocellulose membrane (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5% sodium deoxycholate) and 7.5uL of 4X Laemmli buffer (1610747; BioRad, Hercules, CA) and run on 10-20% Criterion Tris-HCl gels (3450042 ...
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bioRxiv - Immunology 2024Quote: ... Adherent cells were lysed in sodium dodecyl sulfate (SDS) sample lysis buffer (BioRad). Proteins were separated by gel electrophoresis on a 10% Tris-Glycine gel (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... Urea and Sodium dodecyl sulfate (SDS) were purchased from Bio-Rad (Hercules, CA). Acetonitrile and water for nano-LC−MS/MS were purchased from J ...
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bioRxiv - Plant Biology 2020Quote: ... The reactions were carried out in a thermal cycler (Single Block Alpha Unit, DNA Engine®, Bio-Rad Laboratories, Berkeley, CA, USA) with an annealing temperature of 55 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified over the corresponding GST-LRRCC1 fusion bound to Affi-Gel 10 resin (Bio-Rad). Other primary and secondary antibodies used in this study are listed in Table 1.
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bioRxiv - Microbiology 2020Quote: ... Blot was washed 10 minutes (3X) in TBST (0.1% Tween-20) secondary goat anti-rabbit antibody (BioRad, 1721019) was applied (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: 10 μm sections of mouse tumors were stained with primary rat anti-mouse CD68 antibody (MCA1957B, Bio-Rad), and goat anti-human TSP-4 (AF2390 ...
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bioRxiv - Biochemistry 2021Quote: ... D3_Sof protein was immobilized covalently on the surface of one of the vertical channels of the GLC sensor chip (Bio-Rad Laboratories, Hercules, CA USA) to a 100 RU level ...
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bioRxiv - Neuroscience 2020Quote: ... These proteins were then subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad) and transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Protein was separated by 8%–12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (Bio-Rad) and blotted on polyvinylidene difluoride membrane (Merck Millipore) ...
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bioRxiv - Microbiology 2023Quote: Sodium-Dodecyl-Sulphate (SDS) Polyacrylamide Gel Electrophoresis was done in precast gels (Bio-Rad TGX Mini-Protean 4-15% gels with Dual Colour Standard ...
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bioRxiv - Bioengineering 2023Quote: ... GL Sciences, Tokyo, Japan, F10-G-10, 10 × 10 mm2; F10-G-20, 20 × 10 mm2, a polystyrene cuvette: BIO-RAD, Hercules, CA, #1702415, 10 × 10 mm2), and was placed on a suspended sample stage combined with a θ stage (Thorlabs ...
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bioRxiv - Biophysics 2019Quote: ... All custom-made antibodies were purified from serum with an antigen-coupled matrix (Affi-Gel 10 or 15, Biorad). For western blot analysis of other proteins ...
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bioRxiv - Plant Biology 2021Quote: ... dissolved in a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer (Bio-Rad) and separated on 15 % SDS-PAGE gels following the Laemmli method (Laemmli ...
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bioRxiv - Neuroscience 2022Quote: ... A 12% sodium dodecyl sulfate-polyacrylamide gel was prepared according to manufacturer’s protocol (BIO-RAD). For the analysis 32 μl of the production supernatant was mixed with 8 μl 5xLaemmli buffer containing ß-Mercaptoethanol and incubated for 10 min at 95°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... miR-146a-loaded MLNPs were lysed with 0.5% sodium dodecyl sulfate (SDS) (BioRad, Hercules, CA), and loaded onto 2% agarose (Fisher BioReagents ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated in 4–15% sodium dodecyl sulfate/polyacrylamide gel electrophoresis gel (Bio-Rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Bioengineering 2021Quote: ... which were gently transferred using a multi-channel pipette into a semi-skirted 96-well plate before heat-sealing (Bio-Rad PX1™ PCR Heat Sealer, 1814000). Target amplification within each droplet was conducted in the C1000 Touch™ Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Onboard de-waxing was performed in accordance with the standard Leica Bond protocol and staining undertaken using IHC Protocol F with the following adaptations: additional non-specific block prior to primary antibody incubation (10% normal horse serum (Biorad), 1x Casein (Vector Labs ...
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bioRxiv - Neuroscience 2019Quote: ... conjugated secondary antibody (1:10 000) was subsequently applied and visualized by reaction with chemiluminesence reagent (Clarity Western Blotting Substrates Kit, BioRad) on radiography film (Carestream Blue X-ray film).
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bioRxiv - Neuroscience 2021Quote: ... The blots were then washed 3x with TBS-T for 10 min each and incubated with the horseradish peroxidase (HRP) conjugated specific secondary antibodies (1:20,000, Bio-Rad) for 1 h at RT ...
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bioRxiv - Plant Biology 2023Quote: ... HA-tagged proteins were detected using primary antibody anti-HA (1/10 000) followed by incubation with the HRP-conjugated secondary antibody and finally revealed by chemiluminescence (Clarity Western ECL, Biorad) on a Fusion-FX imager (Vilber).