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Citations for Bio-Rad :
51 - 100 of 3617 citations for Sodium Voltage Gated Channel Alpha Subunit 10 SCN10A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: The prepared lysates were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto PVDF membranes (Bio-Rad, USA). The membranes were then blocked and incubated overnight at 4°C with the primary antibodies (as listed in Table S6) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Mini TGX gels (10%, Bio-Rad, Hercules, USA) according to standard protocols and transferred to nitrocellulose membranes (0.2 μm ...
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bioRxiv - Pathology 2021Quote: ... and an antibody (PPG5/10; Bio-Rad Laboratories, Hercules, CA) widely used to measure estrogen receptor beta ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
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bioRxiv - Microbiology 2022Quote: ... the proteins were separated on a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, USA). The blots were blocked in 5% BSA in Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (∼15 μg) were loaded and separated on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad, Hercules, CA), and transferred to polyvinilidene difluoride membranes (PVDF ...
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bioRxiv - Biochemistry 2023Quote: The histones containing residue-specific acetylations or the nucleosomes used for the acetyltransferase activity assay were electrophoresed in a 10–20% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) (DRC, NXV-396HP20) and transferred onto a nitrocellulose membrane (BIO-RAD, 1620112) at 20 V for 10 min by the semi-dry method ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of protein from each lysate were resolved on 10% or 12% sodium dodecyl sulphate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to a polyvinylidene fluoride (PVDF) membrane (Bio-Rad, USA) using a semidry electrophoretic apparatus ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant plasmid pT181-rpvA and the pT181 vector were electroporated into RN4220 (voltage = 2.5 kV, resistance = 100Ω, capacity = 25 μF) using MicroPulserTM Electroporation Apparatus (Bio-Rad respectively ...
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bioRxiv - Developmental Biology 2024Quote: ... at room temperature and constant voltage (100 V) in 1X MOPS Running buffer and transferred to a nitrocellulose membrane (0.45 mM, Bio-Rad) using iBlot2 system (Invitrogen) ...
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bioRxiv - Biophysics 2020Quote: Channels and connected inlet and outlet tubing where primed by flushing 25 μl droplet oil (BioRad) using a 25 μl direct displacement pipette (Gilson ...
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bioRxiv - Microbiology 2020Quote: ... ddPCR reaction mix was then loaded into eight channel disposable droplet generator cartridge (Bio-Rad, Australia). 70 µl of droplet generation oil was added to each channel and placed in the Bio-Rad QX200 droplet generator ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were collected then Gag was immunoprecipitated using an α-RSV antibody and were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and quantified using a PhosphorImager (Bio-Rad). After 35S-Met/Cys labeling for 2.5-hours ...
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bioRxiv - Microbiology 2021Quote: ... and analyzed by Western blot as previously described.21 Densitometry was performed to quantify the cleavage efficiency of full-length spike to S1/S2 subunits using ImageLab 6.0.1 (Bio-Rad #12012931). The average results of two experiments were presented.
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... the two subunits 60S and 40S as well as the very light mRNPs were detected by UV absorbance at 256 nm (BioRad) and each gradient was collected in 10 fractions ...
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bioRxiv - Pathology 2021Quote: ... Hippocampal samples with equal amounts of protein (40 μg) were separated by electrophoresis in 10% sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and transferred onto PVDF membrane (Bio-Rad Labs Inc.). Nonspecific binding sites on the membrane were blocked by incubation with 5% milk in Tris-buffered saline solution containing 0.1% Tween 20 at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty micrograms of proteins for each well were separated on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel with pre-stained molecular weight markers (cat. num. 161-0374; Bio-Rad, Hercules, CA) and transferred to a polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2021Quote: ... total and surface labeled proteins were separated using a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (Bio-Rad, Feldkirchen, Germany). The membrane was blocked for 1 h in PBS-T supplemented with 5% skim milk and afterward incubated with 1:600 primary c-Myc-tag Polyclonal antibody (sc-789 ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then subjected to SDS-PAGE at a constant voltage of 100 V for 1 hour at room temperature and transferred to nitrocellulose membrane (BIO-RAD) at a constant voltage of 30 V at 4°C overnight ...
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bioRxiv - Pathology 2019Quote: ... 20% methanol and MilliQ water) overnight at 4°C in a wet transfer apparatus at 70 mA constant voltage (Bio-Rad). Detection of the chemiluminescence immunochemical reaction was performed on a ChemiDoc ™ XRS + Molecular Imager® (Bio-Rad ...
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bioRxiv - Bioengineering 2022Quote: ... run at 120 V constant voltage (Pharmacia LKB, ECPS 3000/150) using a Hoefer gel system and Precision Plus Protein Kaleidoscope standards (Bio-Rad). Protein and peptide concentrations were determined by direct measurement on a Nanodrop 1000 UV-Vis spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred electrophoretically at a constant voltage of 25 V for 7 min using a Trans-Blot Turbo Transfer System (Bio-Rad). After transfer ...
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bioRxiv - Synthetic Biology 2020Quote: ... under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA). Alternatively ...
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bioRxiv - Cell Biology 2023Quote: ... Transfer was performed for 50 min and a constant voltage of 120 V on a Mini Trans-Blot Cell (Biorad R) system ...
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bioRxiv - Microbiology 2021Quote: ... The amplified samples were read in the FAM and HEX channels using the QX200 reader (Bio-Rad). Each experiment was performed with a negative control (no template control ...
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bioRxiv - Cell Biology 2022Quote: ... The stoichiometry of condensin II subunits for each preparation was checked by 5% SDS-PAGE followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining ...
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bioRxiv - Microbiology 2022Quote: ... Sodium dodecyl sulphate (SDS) electrophoresis grade from BioRad. Fructose ≥99% from Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... SDS (Sodium Dodecyl Sulfate) (Bio-Rad, Cat#1610302), Dulbecco’s MEM (DMEM) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... following which they were subjected to separation via sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8% or 10% gels and the Mini-Protein II apparatus (Bio-Rad, CA, United States). Protein bands were electroblotted onto the polyvinylidene difluoride (PVDF ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An equal amount of total protein was resolved by a 10% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked and incubated with either anti Phospho-(Ser/Thr ...
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bioRxiv - Cell Biology 2021Quote: ... prebound with 10 µL anti-V5 antibody (SV5-Pk1, BioRad Cat# MCA1360G) or 10 µL anti-FLAG antibody (M2 ...
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bioRxiv - Systems Biology 2020Quote: ... Unspecific antibody binding was blocked with PBS/10% AB-serum (Bio-Rad) for 5 min at 4°C prior to staining with CD19 or CD3 ...
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bioRxiv - Biophysics 2024Quote: ... and run on a non-denaturing polyacrylamide gel (10% acrylamide-bis 19:1 in EMSA buffer) in 1X EMSA buffer at constant voltage (150 V for 40 min) using a Mini-PROTEAN® Tetra System (Bio-Rad). To prevent heating during electrophoresis ...
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bioRxiv - Plant Biology 2021Quote: ... were loaded onto an 8-channel DG8TM cartridge for droplet generation in the QX200 Droplet Generator (Bio-Rad). Droplets were transferred into a 96-well plate (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... The gels were visualized separately in Cy3 and Cy5 channels using the ChemiDoc MP imaging system (BIO-RAD).
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and stored at -80 until α5 subunit and its binding partners were immunoprecipitated using SureBeads protein G magnetic beads according to the manufacturer’s protocol (Biorad, Hercules, CA, USA). Western blots (detailed in section 2.8 ...
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bioRxiv - Cell Biology 2021Quote: ... prebound with 5-10 µL anti-V5 antibody (SV5-Pk1, BioRad Cat# MCA1360G) and washed once with PBS before incubation with supernatant (4°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... Antibody/chromatin complexes were de-cross-linked with 10% Chelex-100 resin (BioRad) in water at 93 °C and incubated with RNase A and Proteinase K ...
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bioRxiv - Bioengineering 2021Quote: ... The plate was then transferred and read in the FAM and HEX channels using the QX200 droplet reader (BioRad). Data analysis was performed using the QuantaSoft Analysis Pro Software (Version 1.0.596 ...
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bioRxiv - Molecular Biology 2022Quote: ... and then imaged under the green channel of a ZOE Fluorescent Cell Imager (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The “SYBR/FAM” channel of the CFX96 Touch Real-Time PCR Detection System was used for quantification (Biorad, USA). Cycling conditions were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... and 60 μg purified 6xHis-CNAα copurified with regulatory subunit CNB were resolved by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad, 162-0112). The membrane was stained with Ponceau S solution (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2021Quote: ... The droplets were then read in the FAM channels and analyzed using the QX200 reader (Bio-Rad, Hercules, CA, USA). Primers specific for the Km-resistance gene (Km13fw and Km96rev ...
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bioRxiv - Immunology 2021Quote: ... each ddPCR reaction mixture was loaded into the sample well of an eight-channel disposable droplet generator cartridge (Bio-Rad). A volume of 60 μl of droplet generation oil (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... each ddPCR reaction mixture was loaded into the sample well of an eight-channel disposable droplet generator cartridge (Bio-Rad). A volume of 60 μl of droplet generation oil (Bio-Rad ...
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bioRxiv - Synthetic Biology 2019Quote: ... 96-well Alpha™Unit cycling block and low-profile PCR stripes (Bio-Rad Laboratories GmbH, Muenchen, Germany) were utilized ...
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bioRxiv - Systems Biology 2024Quote: ... buffer + 2% (v/v) Sodium dodecyl sulfate (SDS, Bio-Rad). Proteins were denatured at 95 °C and 600 rpm for 10 min before adding a final concentration of 2% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... a constant voltage of was applied (200-300 V to obtain E = 40-60 V/cm) using a DC power supply (Bio-Rad PowerPac Basic, Hercules, CA). At PAGE completion ...