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Citations for Bio-Rad :
101 - 150 of 1202 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... followed by goat anti-mouse IgG (H+L) alkaline phosphatase (AP)-conjugated (1:3000, Bio-Rad Cat# 170-6520, RRID:AB_11125348) antibody or Alexa Fluor 546 goat anti-mouse IgG (H+L ...
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bioRxiv - Molecular Biology 2019Quote: ... Then the membranes were incubated with goat anti-rabbit IgG (H+L) alkaline phosphatase conjugate (1:10,000 dilution) (BIO-RAD). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated with an Alkaline Phosphatase-conjugated Anti-Digoxigenin antibody and the chemiluminescent signal was revealed using a ChemiDoc MP imaging system (Biorad).
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bioRxiv - Cell Biology 2022Quote: ... cocktail tablets and the protein concentration were determined by Bio Rad Protein assay (Bio-Rad). Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant removed to a new microcentrifuge tube and protein concentration determined using the Bradford assay (Bio-Rad).
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bioRxiv - Plant Biology 2020Quote: ... PCR was performed in 0.5 ml eppendorf-type microcentrifuge tubes on an iCycler (Bio-Rad, Hercules, CA, USA) or Dyad (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... in a final volume of 25µl per tube in a CFX96 Real time PCR system® (Bio-Rad) with the thermal conditions as described below ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were transferred to new tubes and peptide concentrations were quantified using the DC Protein Assay (Bio-Rad). Peptides were labeled using 10-plex TMT reagents as described above and 4 μg of proteins were used for each TMT channel ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were transferred to new tubes and peptide concentrations were quantified using the DC Protein Assay (Bio-Rad). 50 μg of each sample were set aside for TMT labeling ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were transferred to fresh tubes and loaded on a 12% SDS-PAGE minigel for electrophoresis (Bio-Rad) and protein blot analysis.
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bioRxiv - Immunology 2021Quote: ... then blocked with 2% blocking buffer (PBS + 2% non-fat dry milk (Bio-Rad) + 2% goat serum + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... then blocked with 2% blocking buffer (PBS + 2% non-fat dry milk (Bio-Rad) + 2% goat serum + 0.05% Tween-20 ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK). Spots were enumerated using an AID ELISpot reader and software (AID).
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bioRxiv - Cell Biology 2019Quote: ... Western analyses were performed using the alkaline phosphatase system and blots were developed using the AP color development reagents from Bio-Rad. Quantitation of the protein band intensity was done using the IPLab software as described previously (Qiu et al. ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK).
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times with 1X PBS for 5 min and developed with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate in alkaline phosphatase buffer (Bio-Rad).
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bioRxiv - Immunology 2019Quote: ... and 2-Mercapoethanol (BIO-RAD) was added to every protein sample with 5 minutes boiling prior to running on gels for western blots ...
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bioRxiv - Biophysics 2019Quote: ... Bio-Beads SM-2 (Biorad) were added to a 50 mg ml-1 concentration and incubated at 4° C overnight to remove detergent.
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bioRxiv - Cell Biology 2019Quote: ... and 2-Mercaptoethanol (BioRad #1610710) and boiled at 95°C for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... + 2 μl SilentFect (Bio-Rad) was incubated at RT for 5 min before mixing with 2 μl siRNA (20 μM stock ...
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bioRxiv - Cell Biology 2020Quote: ... 2× loading buffer (Biorad, 1610768) was added to hairpin samples ...
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bioRxiv - Microbiology 2020Quote: ... and 2-mercaptoethanol (Bio-Rad), heated at 100°C for 5 minutes ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... and MOMA-2 (Bio-Rad). Plaque size was analyzed by ORO staining to provide contrast ...
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bioRxiv - Physiology 2019Quote: ... and 2-mercaptoethanol (Bio-rad) and heated at 95°C for 5-10 min ...
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bioRxiv - Microbiology 2022Quote: ... + 2-mercaptoethanol (Bio-Rad #1610710) directly to the cell monolayer followed by incubation at 95°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 2 mm cuvette (Bio-Rad). The mixture was recovered for 2-3 h in 1 mL of NB medium at 28℃ and then cultured for 2 h after adding aTc to a final concentration of 200 μg/mL ...
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bioRxiv - Biophysics 2023Quote: ... 2% bis-acrylamide (Bio-Rad), and fluorescent beads (yellow or Texas red with ~0.5 μm diameter ...
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bioRxiv - Molecular Biology 2023Quote: ... + 2 µl SilentFect (Bio-Rad) was incubated at room temperature (RT ...
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bioRxiv - Genomics 2023Quote: ... 0.128mM 2-Mercaptoethanol (BioRad 1610710XTU), and 1X Leukemia Inhibitory Factor (purified and gifted by Barbara Panning Lab at UCSF) ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant were transferred to fresh tube and solubilized protein was measured using a DC protein assay kit (Bio-Rad). For immunoblotting an appropriate volume of 1 x Laemmli (Bio-rad ...
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bioRxiv - Neuroscience 2020Quote: ... The mixture was incubated at 37°C in PCR tubes on a thermal cycler (BioRad DNA Engine PTC-200). After various times 50 µl of the mixture was taken and subject to SEC using a Shodex KW-802.5 column (HICHROM ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then pelleted in a 1.5 mL tube and resuspended in 1 mL phosphate buffered saline pH 7.8 (Bio-Rad) (PBS) ...
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bioRxiv - Genomics 2019Quote: ... we first assembled the 8-strip tubes into 96-well plates for increased throughput (Bio-Rad, RC9601 and MSA5001). For FACS-collected cells we proceeded directly with the obtained 96-well or 384-well plates ...
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bioRxiv - Plant Biology 2021Quote: ... The following components were added to a reaction tube: 9 μL of iQ SYBR Green Supermix solution (Bio-Rad), 1 μL of 5 mM specific primers ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was then transferred into a new tube and protein concentration was determined by BCA assay (Bio-Rad). Immunoblotting was performed using CDCP1(D1W9N ...
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bioRxiv - Neuroscience 2022Quote: ... GFP+ cells were captured into single tube with 100 μl lysis buffer containing Proteinase K and DNase (Bio-Rad). The sorted cells were then incubated for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... then supernatant was transferred to pre-chilled low-adhesion tubes and quantified by Bradford (cat. no. 5000006, Bio-Rad).
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bioRxiv - Immunology 2020Quote: ... the reaction mixture was passed through 2 Bio-Gel P-2 mini columns (1504114, Bio-rad) by centrifugation (3000 rpm ...
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bioRxiv - Microbiology 2019Quote: ... Fractions of 1 ml were recovered from the top to the bottom of each tubes and loaded on a gradient 4-12 % SDS-PAGE Bis-Tris gel (BioRad). For Western Blot analysis ...
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bioRxiv - Neuroscience 2020Quote: ... The mixtures were incubated at 37°C in micro PCR tubes on a thermal cycler (BioRad DNA Engine PTC-200). At the indicated time points ...
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bioRxiv - Neuroscience 2021Quote: ... PCR tubes were placed in a DNA Engine® Peltier Thermal Cycler PTC-200 (Bio-Rad Laboratories, Inc., Hercules, CA), and samples were incubated at 25°C for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... GEMs were transferred to PCR 8-tube strips and GEM-reverse transcription was performed in a C1000 Touch Thermal Cycler (BioRad): 53 °C for 45 min ...
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bioRxiv - Physiology 2019Quote: ... individual cardiomyocytes were immediately aspirated and deposited into separate microcentrifuge tubes containing SingleShot Cell Lysis buffer (Bio-Rad, Hercules, CA) supplemented with DNase ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli Mach1-T1 cells was collected in 1.5 mL centrifuge tubes and then visualized under bright field and imager (ChemiDoc MP Imaging System, Bio-Rad). For imaging the fluorescence of mCherry ...
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bioRxiv - Cell Biology 2022Quote: ... supernatants were transferred to pre-chilled low-adhesion 1.5 mL tubes and quantified by Bradford (cat. no. 5000006, Bio-Rad). Prior to loading ...
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bioRxiv - Cell Biology 2022Quote: ... supernatants were transferred to pre-chilled low-adhesion 1.5 mL tubes and quantified by Bradford (cat. no. 5000006, Bio-Rad). After quantification ...
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bioRxiv - Genetics 2022Quote: ... the antibodies were prepared by mixing 3 ug of antibody HNRNPK and IgG (as a negative control in a separate tube) with 650 ul of Dilution Buffer and 50 ul of Surebeads Protein G magnetic Beads (BioRad) and left in rotation for 30 minutes at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... All steps were done manually using 8-strip PCR tubes and PCR reactions were carried out on a 96-plate thermal cycler (Biorad). Libraries were pooled and sequenced on a Nextseq550 sequencer (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction mixtures were held at 10°C until the PCR tubes were removed from the thermocycler (C1000 Touch, BioRad). After ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then transferred to fresh low- protein binding tubes and antibody-protein complexes were eluted by incubating in 60 μL 1x Laemmli Sample Buffer (Biorad, cat ...