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Citations for Bio-Rad :
1 - 50 of 1202 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... phosphatase inhibitor cocktail and diluted in 4X Laemmli buffer (BioRad) containing β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2024Quote: ... discs from third-instar larvae were dissected in Ringer’s Solution supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail on ice and then lysed in 2x Laemmli Sample Buffer (Biorad #1610737EDU) and heat denatured at 95°C for 5 minutes.
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bioRxiv - Cell Biology 2021Quote: ... ± phosphatase inhibitor cocktail 3) and incubated for 30 min on ice before addition of MnCl2 and λ-phosphatase (Bio-Rad) to the appropriate samples for 30 min at 30°C ...
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bioRxiv - Cancer Biology 2019Quote: ... lysis buffer supplemented with protease inhibitor cocktail (Pierce) and quantified (BioRad DC protein assay). 30 μg of lysate was loaded to SDS-polyacrylamide gels ...
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bioRxiv - Immunology 2022Quote: ... supplemented with Protease and Phosphatase Inhibitor and loaded on 4–15% SDS-PAGE gels (Bio-Rad Laboratories, Inc.). Proteins were transferred on a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2019Quote: ... was transferred to a new tube with 50 μL 2 M HCl and boiled for 2 hours at 95°C in a C1000 thermocycler (Bio-Rad) to hydrolyze all glucans to glucose ...
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bioRxiv - Genetics 2019Quote: ... and 1x phosphatase inhibitors and run as previously reported (Blauwendraat et al. 2018a) on 4-20% Criterion TGX gels (Bio-Rad) in Tris-glycine running buffer and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: E15.5 forebrains were dissected/frozen on dry ice and then lysed in standard RIPA buffer with protease and phosphatase inhibitors and combined with Laemmli buffer (BioRad 1610737EDU) containing 2-Mercaptoethanol and incubated at 95°C for 5 minutes ...
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bioRxiv - Physiology 2024Quote: ... Pierce™ Protease& Phosphatase Inhibitor Mini Tablets) and final protein concentration was determined with BCA protein Assay (Biorad Laboratories, Feldkirchen, Germany). Heart tissue samples of the infarct areas were excised (2 weeks or 8 weeks post-surgery ...
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bioRxiv - Genetics 2021Quote: ... protease inhibitor cocktail) and protein concentrations were determined by Bradford protein assay (Bio-Rad Laboratories, Inc. Hercules, CA, USA) and absorbance was measured at 595 nm on the Tecan Infinite® 2000 PRO plate reader (Tecan ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were diluted 1:10 in RIPA Lysis and Extraction buffer (Thermo Fischer) supplemented with protease inhibitor cocktail (Fischer Scientific) for use in BCA protein assay (BioRad). BCA protein assay was performed in triplicate for each diluted sample following manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Chemiluminescent alkaline phosphatase (AP)-conjugated (BioRad) or fluorescent IRDye 800CW (goat anti-rabbit ...
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bioRxiv - Microbiology 2023Quote: ... brucei PFs were prepared in 1% Triton X-100 in PBS with 1X protease inhibitor cocktail (Bio-Vision) and mixed in 4X Laemmli buffer (Bio-Rad) with 710 mM β-mercaptoethanol and heated for 5 min at 95°C ...
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bioRxiv - Genomics 2021Quote: ... at 1.8 kV distributed over 2 cuvettes (0.1 cm gap width) per tube using a Micropulser Electroporator (Bio-Rad Laboratories). 10-fold serial dilutions of a 10 μL aliquot were plated on LB plates with ampicillin at 100 μg/mL to assess electroporation efficiency ...
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bioRxiv - Biophysics 2021Quote: ... The solution was then transferred to a tube containing ∼30- 50 mg of Bio-Beads (Bio-Beads SM-2, BioRad) that were previously equilibrated in 20 mM Tris-HCl pH 7 ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1X phosphatase inhibitor cocktail and equal amounts of protein samples were loaded and run in a 4 % to 20 % SDS gel (Bio-Rad Laboratories). Protein was transferred to a polyvinylidene fluoride membrane (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... 1 cOmplete™ protease inhibitor cocktail EDTA free tablet) and protein concentrations determined using the DC protein assay (Bio-Rad, Hercules, CA). Oxygen Consumption Rate (OCR ...
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bioRxiv - Bioengineering 2019Quote: ... generated droplets or Gelbeads were extracted into PCR tubes (0.2 mL individual PCR tubes, BioRad) and incubated in a thermal cycler (T100 ...
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bioRxiv - Plant Biology 2023Quote: ... alkaline phosphatase-conjugated antibodies (Bio-Rad, USA) were used for fluorometric detection of the immune-reactive bands with the AttoPhos system (Promega ...
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bioRxiv - Cell Biology 2021Quote: Total cell lysates were prepared in Laemmli SDS-sample buffer supplemented with Protease Inhibitor Cocktail (Nacalai Tesque, Kyoto, Japan) and protein concentrations were quantified by the DC protein assay (Bio-Rad, CA, USA). Protein concentrations were normalized if necessary and boiled for 5 min with 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA (25 ng) were used for qRT-PCR using 2 x IQ SYBR Green supermix cocktail (Bio-Rad, cat. 1708880). Primers used for all genes are listed in the following Table ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies conjugated with alkaline phosphatase were used with an ImmunStar™-Alkaline Phosphatase (AP) Western Chemiluminescent Kit (Bio-Rad) for signal development ...
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bioRxiv - Microbiology 2020Quote: ... followed by ExtrAvidin alkaline phosphatase conjugate and alkaline phosphatase substrate: p-nitro blue tetrazolium chloride enhanced 5-bromo-4chloro-3-indolyl phosphate (Bio-Rad). The membranes with the transferred rgp120 were incubated with the HRP-conjugated V5 antibody (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... a small implant of the thallus of interest (0.5 cm2) was set in 2 mL tube containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
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bioRxiv - Plant Biology 2022Quote: ... 50 mg of powder was transferred into a 1.5 mL microcentrifuge tube with 150 ul of 2 x Laemmli buffer (Bio-Rad, Hercules, CA, USA). The sample was immediately vortexed for 1 min followed by incubation at 70°C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... 0.2 mL microcentrifuge tubes (Bio-Rad Laboratories) were coated with 10 mM DOPA-Tet ...
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bioRxiv - Physiology 2022Quote: ... Tubes were placed in a Thermocycler (BioRad). Samples were cycled at 50 °C for 30 seconds ...
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bioRxiv - Genetics 2022Quote: ... of each mating type of the strains of interest were placed in 2 mL tubes containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were visualized by alkaline phosphatase conjugate substrate (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Spots were visualised using alkaline phosphatase substrate kit (Bio-Rad) and the reaction stopped using water ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized by alkaline phosphatase conjugate substrate (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: Cells were resuspended in regular culture media in 8-strip PCR tubes with 50□μl per tube and received heat shock (HS) in a thermal cycler (Bio-Rad, 1851148) for various durations before returning to normal culture condition ...
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bioRxiv - Biochemistry 2024Quote: ... and secondary goat antimouse IgG antibody conjugated to Alkaline Phosphatase (BioRad) were used.
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bioRxiv - Cell Biology 2020Quote: ... via alkaline phosphatase and quantified with ImageLab 5.0 (ChemDoc MP, BioRad, USA). The integrated intensity was used to estimate the expression level of tagged MVI compared to the endogenous level.
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bioRxiv - Microbiology 2020Quote: ... specific proteins were visualized with alkaline phosphatase conjugate substrate kit (Bio-Rad) and images were scanned with Image Scanner (Amersham Pharmacia Biotech ...
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bioRxiv - Biochemistry 2022Quote: ... and secondary goat anti-mouse IgG antibody conjugated to Alkaline Phosphatase (BioRad) were used.
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bioRxiv - Immunology 2022Quote: ... To each well 50 µL/well of alkaline phosphatase substrate (Bio-Rad) was added and incubated at RT for approximately 15 min or until spots developed ...
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bioRxiv - Neuroscience 2022Quote: ... Spots were visualised with alkaline phosphatase conjugate substrate (Bio-Rad; 170-6432) and PK-resistant infected cells were counted using the Bioreader 5000-Eβ (BioSys Karben ...
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bioRxiv - Physiology 2023Quote: ... The alkaline phosphatase conjugated goat anti-mouse IgG antibody (BioRad; Hercules, CA) was used as secondary antibody (with all of the primary antibodies described here ...
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bioRxiv - Microbiology 2021Quote: ... and pelleting were conducted in racked titer tubes (Bio-Rad; 2239391) on ice or at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... tubes were aspirated and Bio-Plex Cell-Lysis Kit (Bio-Rad) was added and spheroids were then sonicated ...
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bioRxiv - Neuroscience 2022Quote: ... A red Bio-Rad 2100MPD photomultiplier tube (Bio-Rad, Hercules, CA, USA) (570 – 650 nm ...
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bioRxiv - Synthetic Biology 2023Quote: ... in 0.2 ml 8-Tube PCR Strips (Bio-Rad™, clear #TBS0201) with 0.2 ml Flat PCR Tube 8-Cap Strips (Bio-Rad™ ...
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bioRxiv - Immunology 2020Quote: ... The reaction tubes were transferred into a C1000 Touch Thermal Cycler (Bio-Rad) or UNO96 HPL Thermal Cycler (VVR ...
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bioRxiv - Synthetic Biology 2021Quote: ... All tubes were imaged simultaneously with a ChemiDoc MP Imaging System (Bio-Rad) using the ‘Fluorescein’ preset (blue epi excitation ...
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bioRxiv - Microbiology 2021Quote: ... or 0.2 mL white 8-tube PCR strips and optical caps (Bio-Rad). For bacterial cultures ...
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bioRxiv - Cell Biology 2021Quote: ... cocktail tablets and the protein concentration were determined by Bio-Rad Protein assay (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... cocktail tablets and the protein concentration were determined by Bio-Rad Protein assay (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2 M 2-Mercaptoethanol (Bio-Rad), and boiled for 3 min ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... and alkaline phosphatase-conjugated anti-rabbit serum from goat as secondary antibody (1706518, Biorad, Munich, Germany). The signals were visualized by treating the blot with BCIP/NBT substrate (B1911 ...