Labshake search
Citations for Bio-Rad :
1201 - 1250 of 2247 citations for Phosphatidylinositol N acetylglucosaminyltransferase subunit Q PIGQ Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... The plates were then washed again and incubated for 30 minutes with 50 µl of either a 1:7000 dilution of goat IgG conjugated with horseradish peroxidase (HRP) having anti-chicken IgY specificity (Biorad), or 1:3000 dilution of goat IgG conjugated with horseradish peroxidase (HRP ...
-
bioRxiv - Molecular Biology 2024Quote: ... diluted to a concentration of 1/5,000 in Can Get Signal Solution 2 (TOYOBO) or HRP-conjugated goat anti-rabbit IgG (Bio-Rad) diluted to a concentration of 1/3,000 in 5% skim milk at room temperature for 1 hour ...
-
bioRxiv - Cell Biology 2024Quote: ... Western blot chemiluminescent substrate or Femtolucent plus HRP chemiluminescent reagents and imaged on a ChemiDoc™ Imaging System (Bio-Rad).
-
bioRxiv - Cell Biology 2024Quote: ... After incubation the blot was again subjected to three washes and then developed using HRP-Luminol based chemiluminescence (Clarity, Biorad) in Vilber-Lourmat chemidoc machine ...
-
bioRxiv - Cell Biology 2024Quote: ... then incubated for 1 h at room temperature with 0.1 μg/ml HRP- conjugated goat anti-rabbit (BioRad, 162-0177) in blotting buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... For MARCO and TLR2 antibody blockade either 0.8 µg of MARCO antibody (Bio-Rad) 34 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Antibodies used for SBA: Alexa 488 labeled mouse anti-human CD9 antibody (MCA469A488, Biorad), PE-labeled mouse anti human CD63 antibody (12-0639-42 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The secondary antibody was a goat anti-rabbit antibody conjugated to horseradish peroxidase (BioRad). Bound antibody was visualized by incubation in Clarity ECL substrate (BioRad ...
-
bioRxiv - Biochemistry 2021Quote: ... secondary antibody (Biorad #170-6515), H3-HRP (Abcam #ab21054) ...
-
bioRxiv - Systems Biology 2023Quote: ... Horseradish-conjugated secondary antibodies (BioRad) were diluted 1:5000 in blocking buffer and membranes were incubated 1 h at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibodies to TGN46 (AHP500G, Biorad) and GM130 (610822 ...
-
bioRxiv - Bioengineering 2024Quote: ... anti-V5 antibody (BioRad MCA1360) was captured on Dynabeads pA (Life Technologies 10002D ...
-
bioRxiv - Cell Biology 2024Quote: ... coli antibodies (Bio-Rad Laboratories) at 1:100 dilution in 2% BSA in PBS were applied to fixed ...
-
bioRxiv - Physiology 2020Quote: ... versions with an N-terminal cysteine were additionally made in order to conjugate a TMR-C5-maleimide Bodipy dye (Bio-Rad, CA, USA), to make fluorescent TMR-C5-maleimide-SPTISITAPIDVLRKTWAKENMRKQMQINREYLKNLQamide (DH37-F ...
-
bioRxiv - Microbiology 2021Quote: ... 40 μg of ΔORF3-E mNG RNA and 20 μg of N-gene RNA were electroporated into 8×106 Vero-ORF3-E cells using the Gene Pulser XCell electroporation system (Bio-Rad, Hercules, CA) at a setting of 270V and 950 μF with a single pulse ...
-
bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
-
bioRxiv - Molecular Biology 2024Quote: ... separated by gel electrophoresis and the band at ∼220bp excised using Freeze ’N Squeeze™ DNA Gel Extraction Spin Columns (Biorad cat#7326166). The DNA was further concentrated using Zymo DNA clean and concentrate columns (Zymo cat#D4014 ...
-
bioRxiv - Cancer Biology 2021Quote: ... An anti-mouse IgG secondary antibody (diluted 1:5000) conjugated with horseradish peroxidase (HRP) was used to reveal the immunoblot bands by chemiluminescence (Clarity Western ECL substrate, Bio-Rad) following the supplier’s instructions ...
-
Systemic impact of the expression of the mitochondrial alternative oxidase on Drosophila developmentbioRxiv - Biochemistry 2022Quote: ... the membranes were incubated with a 1% nonfat milk/PBST solution containing the secondary HRP-conjugated goat anti-rabbit (1:10,000, Bio-Rad, USA) and anti-mouse (1:10,000 ...
-
bioRxiv - Bioengineering 2021Quote: ... Both secondary antibodies are HRP-conjugated and were visualized using West Pico Plus HRP substrate (Thermo Fischer) and measured with a ChemidoC XRS image analyzer (Bio-Rad).
-
bioRxiv - Neuroscience 2021Quote: ... the membrane was washed and submerged in chemiluminescent HRP substrate while the fluorescent signals of the blots were captured by gel imaging analysis system (Bio-rad). The membrane was incubated with primary antibody of β-actin (1:1000 ...
-
bioRxiv - Microbiology 2020Quote: ... HRP activity was detected with a chemiluminescence detection system (Li-COR Imaging System) by using Clarity chemiluminescent detection system HRP substrate (Bio-Rad). Each Western blot also included 3 μL of a Page Ruler Plus pre-stained protein ladder (Thermo ...
-
bioRxiv - Neuroscience 2020Quote: ... Immunoblots were developed using Luminata Crescendo Western HRP Substrate and digital signals were imaged and analyzed using ChemiDoc Imaging system (Bio-Rad) and ImageLab software (BioRad) ...
-
bioRxiv - Plant Biology 2021Quote: ... The blot was washed 3 times with TBST and incubated for 30 min in 1% milk prepared in TBST with anti-mouse HRP conjugate (Bio-Rad). Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration (Thermo ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were washed thrice before the addition of horseradish peroxidase (HRP)-conjugated anti-mouse IgG (H+L) (Bio-rad, 170-6516) at 1:3,000 ...
-
bioRxiv - Biochemistry 2021Quote: ... both used at a concentration of 1:200 were followed by a one-hour incubation with secondary Goat anti-(Rabbit IgG-HRP conjugate) (#1706515, Bio-Rad) at a concentration of 1:8000 at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... at room temperature [goat anti-mouse 170-6516 or goat anti-rabbit 170-6515 IgG (H+L) HRP conjugate (Bio-Rad)] were all used at 1:2000 in 3 % BSA in 0.1 % Tween 20 (PBS/T) ...
-
bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...
-
bioRxiv - Molecular Biology 2024Quote: ... blots were washed three times in TBST for 5 minutes each and incubated with an anti-mouse HRP secondary (Bio-Rad) at 1:2000 dilution for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Excess bound secondary antibody was then removed by washing and HRP activity was visualized by enhanced chemiluminescence with Clarity ECL Western Blotting Detection System (Bio-Rad). Band intensity was evaluated with the Image J software using either β-actin or α-tubulin band density as loading control ...
-
bioRxiv - Microbiology 2023Quote: ... Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380) diluted 1:10,000 in TBST ...
-
bioRxiv - Immunology 2023Quote: ... plates were washed thrice before addition of horseradish peroxidase (HRP)-conjugated anti-mouse IgG (170-6516; Bio-rad, Hercules, California, USA), anti-mouse IgG1 (ab97240 ...
-
bioRxiv - Microbiology 2024Quote: ... and the same transferred membrane was stripped and re-probed for Staphylococcus Protein A detection with HRP-conjugated Anti-rabbit (Bio-Rad) at 1/3000 dilution.
-
bioRxiv - Neuroscience 2024Quote: ... Detection was carried out using the Immobilon Forte Western HRP substrate (MilliporeSigma) and images were acquired with ChemiDoc Imaging system (Bio-Rad). Data was quantified by densitometric analysis using ImageJ software and individual band intensities were normalized to β-actin.
-
bioRxiv - Genomics 2024Quote: ... The membrane was washed thrice with 1X PBST for 10 minutes each and developed using Clarity-max chemiluminescent HRP substrate (BIO-RAD) using the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Bound antibodies were detected with the species-appropriate secondary antibodies by a ChemiDoc XRS (Biorad) imager after enhanced chemiluminescence (Supersignal West Pico Plus ...
-
bioRxiv - Systems Biology 2021Quote: ... which contained 200 nM of TSWV-N or thrips internal reference Actin gene primers in the SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). Samples with missing and/or high (>35 ...
-
bioRxiv - Microbiology 2023Quote: ... ddPCRs for each sample (n=50) were prepared in an initial volume of 72 µl comprising 37.5 µl EvaGreen Supermix (QX200; Bio-Rad, South Granville, NSW, Australia), 31.5 µl sterile water ...
-
bioRxiv - Plant Biology 2024Quote: RNA samples resolved in denaturing polyacrylamide gels were transferred to positively charged nylon membranes (Cytiva, Hybond-N+, catalog no. 45-000-850) using a semi-dry Trans-Blot Transfer System (Bio-Rad, catalog No. 1703940) in 0.5X TBE at constant 20 V for 45 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected using the EZ-ECL Chemiluminescence Detection Kit for HRP (Biological Industries) and ChemiDoc XRS+ Gel Documentation System (Bio-Rad Laboratories). Images were taken using the ImageLab™ software (Bio-Rad).
-
bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
-
bioRxiv - Cell Biology 2021Quote: ... The membrane was washed three times for 10 minutes with 1x PBS and incubated with Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad, #172-1011). Chemiluminescence was induced by incubation with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher ...
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed three times in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1,706,515, 1:10,000) secondary antibodies for 2 h at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
-
bioRxiv - Molecular Biology 2021Quote: ... Densitometry of the blots was detected using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA). Information on the primary antibodies used has been listed and provided (Table EV4).
-
bioRxiv - Molecular Biology 2021Quote: ... The membrane was exposed using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA).
-
bioRxiv - Microbiology 2024Quote: ... SigA proteins were detected using anti-SigA85 (1:10,000 dilution) combined with anti-rabbit-HRP secondary (Bio-Rad, #1706515, 1:5,000 dilution) antibodies ...
-
bioRxiv - Cancer Biology 2022Quote: ... The membrane was visualised by chemiluminescence on Biorad ChemiDoc MP Imager after incubation with goat anti rabbit HRP conjugate (Biorad; 170-6515) or goat anti-mouse-HRP conjugate (Biorad ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the membrane was washed using TBST and incubated for 1 h in a 1:20,000 dilution in 5 % milk of Immun-Star Goat Anti-Mouse (GAM)-HRP conjugate from Bio-Rad (Ontario Canada). Three washes were done ...