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Citations for Bio-Rad :
1151 - 1200 of 2247 citations for Phosphatidylinositol N acetylglucosaminyltransferase subunit Q PIGQ Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... from colonic samples of DR3.IL17A-/- (n = 5) and DR3 mice (n = 5) were reverse transcribed to cDNA using the High-Capacity iScript cDNA synthesis kit (Bio-Rad). qPCR reactions were then carried out in triplicate using 50 ng of cDNA and the Applied Biosystems Power SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified RNA was separated on a 1.7% agarose–formaldehyde denaturing gel and was then transferred to a nylon membrane (Hybond-N+, Cytiva) in 10× SSC buffer using a model 785 vacuum blotter (Bio-Rad). RNA was cross-linked to the membrane using an ultraviolet (UV ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... at which point the crushed gel could be transferred from the tube lid to a DNA spin column (Freeze ‘N’ Squeeze, Bio-Rad) with tweezers ...
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bioRxiv - Cell Biology 2020Quote: ... ECL blotting reagent was used to visualise HRP and chemiluminescent signal was detected using the ChemiDoc MP system (BioRad) as per the manufacture’s instruction.
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bioRxiv - Cancer Biology 2020Quote: ... The immunoblotted bands were detected by the addition of horseradish peroxidase (HRP) substrate (Bio-Rad, cat. no. 170-5601) and captured on a ChemDoc XRS+ System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... and Immobilon Western Chemiluminescent HRP Substrate was used for imaging with the ChemiDoc Touch imaging system (Bio-Rad, 1708370).
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated anti-rabbit and anti-mouse were used for detection using the chemiluminescence detection kit Clarity ECL (Biorad).
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bioRxiv - Genetics 2020Quote: ... or by anti-Smc3 K112/K113 Acetylation (1:1,000, gift from Dr. Katsuhiko Shirahige) in combination with goat anti-mouse HRP (1:10,000) (Bio-Rad) and ECL prime (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was developed with Immobilon Crescendo Western HRP substrate (MilliporeSigma) and imaged using an ChemiDoc Imaging System (Biorad).
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bioRxiv - Immunology 2021Quote: ... Bound PRRSV-2-specific IgG was detected using goat anti-pig IgG conjugated with horseradish peroxidase (HRP; Bio-Rad) diluted at 1:10,000 in blocking buffer for 1 h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated goat anti-rabbit IgG (1:5000; 1706515, Bio-Rad) at RT for 1 h followed by chemiluminescent detection with ECL Substrate (1705060 ...
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bioRxiv - Developmental Biology 2023Quote: ... Chemiluminescence detection was performed using Luminata Forte Western HRP substrate (MilliporeSigma, WBLUF0100) and a ChemiDoc Touch Imaging System (BioRad). Results shown are representative from at least three independent experiments performed.
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bioRxiv - Developmental Biology 2023Quote: ... and then the membrane signals were visualized by a chemiluminescent HRP substrate reagent (Bio-rad Laboratories, Hercules, CA, USA) and images were captured with Tanon5200 Imaging System (Biotanon ...
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bioRxiv - Developmental Biology 2024Quote: ... including either Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (1/50,000; Bio-Rad #1721019 or Cell Signaling #7074) or Goat Anti-Mouse IgG (H+L)-HRP Conjugate (1/50,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 dilution) followed by Goat anti-mouse IgG (H+L) HRP conjugate at 1:1000 (Biorad 172-1011). The number of infected cells were revealed using True Blue™ peroxidase substrate (KPL 50-78-02 ...
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bioRxiv - Plant Biology 2024Quote: ... α-StrepTag antibodies (Biorad). After transferring to nitrocellulose membrane ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... coli antibodies (BioRad, ON) or rabbit anti-Salmonella O antiserum group B antibodies (Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: V5-antibody (Bio-Rad) was functionalised with a 20 kDa biotin-PEG-SVA linker (Laysan Bio ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plaques of FCoV were stained with specific antibodies (primary antibody: mouse anti feline coronavirus monoclonal antibody at 1:500 dilution solution, Bio-Rad; secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Final panning using antibodies against Thy1.2 (CD90.2) (#MCA02R; Bio-Rad Antibodies, Hercules, CA) positively selected for RGCs ...
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bioRxiv - Genetics 2024Quote: ... Membranes were incubated with primary antibodies and peroxidase-conjugated secondary antibodies (Bio-Rad) using an iBind Flex Western Device (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples (n = 80 embryos / lane) were run on a precast SDS-PAGE gel (4-20% MP TGX Stain-Free Gel, Bio-Rad 4568093), with a Precision Plus Protein WesternC Stds ladder (Bio-Rad 1610376) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μL 5 N NaOH solution was added to and the absorbance was read at 405 nm with a reference filter of 620 nm by iMark Microplate Reader S/N 20255 (Bio-Rad, USA).
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bioRxiv - Synthetic Biology 2020Quote: ... Target bands were excised on a blue light transilluminator and purified with a Quantum Prep® Freeze ‘N Squeeze spin column (Bio-Rad). The eluate was further purified with a magnetic bead clean-up (Agencourt® AMPure® XP ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was slot blotted onto a Hybond N+ membrane with the help of a dot-blot apparatus (Bio-Rad, 170-6545) and subsequently hybridized with a biotin-labeled (TTAGGG)3 probe synthesized by Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was determined by Bradford measurement (64) according to the manufacturer’s instructions (Bio-Rad, cat n° 5000006).
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bioRxiv - Physiology 2023Quote: ... in duplicates in 384-qPCR plates (n = 5 plates) and qPCR were performed with a Biorad instrument (CFX-384, Biorad, Hercules, USA). We used Recombination Activating Gene 1 (RAG1 ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Developmental Biology 2020Quote: ... Protein bands were visualised by triggering the HRP-catalysed chemiluminescent reaction catalysed through addition of the “Clarity” (TM) Western ECL Substrate (BioRad) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane was treated with ECL (Immobilon Western chemiluminescence HRP substrate) for 1 min and visualized with the ChemiDoc MP imaging system (BioRad). Exposure was optimized for each blot.
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bioRxiv - Biochemistry 2022Quote: ... HRP-conjugated anti-mouse was incubated for 1 h at room temperature and visualized by enhanced chemiluminescence (Clarity Substrate, Biorad) using Amersham Imager 680.
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bioRxiv - Biochemistry 2022Quote: Following a series of TBST washes the blots were visualized using Luminata Forte HRP detection reagent and imaged on a gel scanner (BioRad) for chemiluminescence ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were washed a few times in TBST before HRP activity was visualized using Clarity Western ECL Substrate (Bio-Rad) on a ChemiDoc (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated 3 h with a solution containing a secondary goat-HRP anti rabbit (1:10 000) (Biorad) (milk 0.5 % TBST) ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 hour at RT with 1:10,000 goat anti-rabbit horseradish peroxidase (HRP) (Bio-Rad, Cat# STAR208P). The membrane was washed 3 times for 5 minutes with TBST ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for one h at room temperature (RT) with horseradish peroxidase (HRP)-coupled secondary Abs at 1:2000 for one h (all secondary Abs from Biorad, raised in goatanti-rat HRP Cat # 5204-2504 and anti-mouse HRP Cat # 1721011) ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated with BioRad clarity western ECL substrate and Millipore HRP substrate solutions and then imaged in a ChemiDoc MP from BioRad. Some blots were then stripped with 25 mM glycine-HCl and 1% SDS (pH 2.0 ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed three times before the addition of HRP-conjugated anti-mouse IgG (H+L; Bio-Rad; 170-6516) at 1:3,000 and anti-mouse IgG2b (ab97250 ...
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bioRxiv - Cancer Biology 2024Quote: ... Chemiluminescence was performed using Western HRP Substrate and imaged using the Bio-Rad ChemiDoc MP Imaging System (Bio-Rad Laboratories) and Image Lab software.
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bioRxiv - Immunology 2024Quote: ... rabbit anti-FLAG-HRP (clone D6W5B, CellSignalingTechnology #86861S, RRID:AB_2800094) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P, RRID:AB_609796) detection Abs were added for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Precision Plus Protein™ WesternC™ Blotting Standards and Precision Protein™ StrepTactin-HRP Conjugate (Bio-Rad, 1610376 and 1610381) were used ...
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bioRxiv - Cell Biology 2022Quote: ... The signal was detected by enhanced chemiluminescence reagents (Immobilon Crescendo Western HRP substrate, Merk) was imaged with a ChemiDoc MP Imaging System (Biorad). Ponceau S staining (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... This step was followed immediately by an hour incubation with 1:5000 dilution of Goat α-mouse-HRP (Bio-Rad) in TBST ...
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bioRxiv - Biochemistry 2023Quote: ... and Mouse IgG HRP-linked whole Ab (Cytiva), then imaged using (ECL Select, Cytiva) with a ChemiDoc MP (Bio-Rad). For gel quantification ...
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bioRxiv - Microbiology 2023Quote: ... followed by three-times washing with TBST for 5 min and subsequent incubation with the goat anti-rabbit IgG (H+L)-HRP conjugate (BioRad) (diluted 1:3000 in TBST ...
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bioRxiv - Biophysics 2023Quote: ... HRP signal was detected with Amersham ECL Select Western Blotting Detection Reagent (Cytiva) using a ChemiDoc Imaging System (Bio-Rad) in Protein Blot -Chemiluminescence setting.
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bioRxiv - Neuroscience 2023Quote: ... HRP is detected by a chemiluminescence assay using the kit Clarity Western ECL Substrate (Bio-Rad, Marnes-la-Coquette, France), whose light emission is captured by the camera of the device ChemiDoc Touch (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... incubated in Radiance ECL HRP substrate (Azure Biosystems) for 2 min and visualized using the chemiluminescent setting on a ChemiDoc XRS+ (Biorad).
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bioRxiv - Immunology 2024Quote: ... and incubated in Radiance ECL HRP substrate (Azure Biosystems) for 2 min before visualization using the chemiluminescent setting on the ChemiDoc XRS+ (Biorad).