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Citations for Bio-Rad :
1201 - 1250 of 1641 citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 μg of protein samples and 3-5 μL of Precision Plus Protein Dual Color Standards (BIO-RAD, #1610374) were loaded in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... bovis BCG-mCherry) respectively at an interval of 24 hours daily for 5 days using a fluorescence multimode plate reader (Biorad). An MOI of 1 ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Protein samples were loaded on 4-15% polyacrylamide gels (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... followed by three-times washing with TBST for 5 min and subsequent incubation with the goat anti-rabbit IgG (H+L)-HRP conjugate (BioRad) (diluted 1:3000 in TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed in 4 x 5 minutes in 0.2% TBS tween before incubating in Clarity™ Western ECL Substrate (BioRad) for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were washed at least three times for 10 minutes with 1x TBST solution followed by 5 minutes incubation at room temperature with either Clarity Western ECL (BioRad). Immunoblots were developed using an Amersham ImageQuant 500 CCD imaging system (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times for 5 min with 1X TBS 0.1% Tween 20 and unsaturated fluorescent signal was acquired using Chemidoc MP imager (Bio-Rad) with identical settings for samples to be compared within an experiment.
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bioRxiv - Cell Biology 2023Quote: ... 150ul of the suspension was filtered through a 40μM cell strainer at 40G for 5 minutes to remove clumped cells and subsequently used for analysis on the ZE5 flow cytometer (Biorad). Analysis of gene expression was analyzed using the Easyflow MATLAB script (https://antebilab.github.io/easyflow/) ...
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bioRxiv - Immunology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Biophysics 2023Quote: ... The blots were finally washed thrice with TBST with 5 minute incubations before adding the ECL substrate solution (1705061, Biorad). Chemiluminescence images were acquired on a Chemidoc™ imaging system (Biorad) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 15 minutes at 50°C and 5 minutes at 85°C in T100 Thermal Cycler (1861096, Bio-Rad). Real-time qPCR reactions were performed using SsoAdvanced Universal SYBR® Green Supermix (1725274 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with secondary antibody goat anti-rabbit conjugated to HRP (1:5000, 5% wt/vol milk in TBS-T; #170-6515, BioRad) for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run for 5 min at 130 V followed by 55min min at 180 V (PowerPac Basic Power Supply (Biorad)) ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 μl of this cell suspension was then placed on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) TGY2X pad prepared on a cover glass using a gene frame (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Detection of proteins was achieved following 5 min incubation with Enhanced Chemiluminescence (ECL) reagent and exposed using a ChemiDoc imaging system (BioRad). Loading control was performed by normalizing the target protein to the stain-free images of total protein ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were boiled at 95 °C for 5 min before being loaded onto a polyacrylamide gel (Cat# 4561094, Bio-Rad). Proteins were blotted onto a PVDF membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was diluted 1:4 and 4.3 µL of cDNA was mixed with 5 µL iTaq Universal SYBR Green Supermix (BioRad 1725124) and 0.7 µL of 5 µM forward and reverse qPCR primers targeting the gene of interest (333 nM final primer concentration ...
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bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
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bioRxiv - Developmental Biology 2024Quote: ... PVDF membranes were blocked for 1 hour in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4 °C with antibodies listed in Supplementary Table 2 in 5% BSA in TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... All samples collected were heated to 95°C for 5 minutes before resolving on a 12% mini-PROTEAN precast gel (BioRad). A Western blot using polyclonal EssR antiserum was conducted following the protocol outlined in the Western blot method section below ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was then transferred to a 1 mm cuvette (ice chilled) for electroporation at 1.5 kV for 5 ms using an electroporator (Bio-Rad). Electroporated samples were mixed with 1 ml of SOC medium and transferred into a culture tube containing 1 ml of Brucella broth (BD) ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies to the membrane for 1 h in the presence of 5% milk followed by four washes in TBS-T and developing with ECL substrate (1705061; BioRad). Blot signal was captured using the same BioRad ChemiDoc™ Touch Gel Imager ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was blocked with B-TBST (TBS buffer with 0.05% Tween-20 and 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were then blocked with B-TBST (TBS buffer with 0.05% Tween-20 and supplemented with 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were then blocked with B-TBST (TBS buffer with 0.05% Tween-20 and supplemented with 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
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bioRxiv - Neuroscience 2024Quote: ... 72°C (30s) and 1 cycle of 72°C for 5 min (C1000 Touch Thermal Cycler, Bio-Rad, Hercules, CA). 2–5 μl of the PCR reactions were subjected to gel electrophoresis using a 3% agarose gel in tris-borate-ethylenediamine tetraacetic acid buffer ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... Expression studies were further carried out using 100 ng of cDNA template and 0.5 µM (each) of specific primers in a Bio Rad CFX96 real time PCR with SyBr Green iTaq Mix (Biorad). Data generated was further analysed using 2-ΔΔCT method ...
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bioRxiv - Neuroscience 2024Quote: ... Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad) using a turbo transfer system (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were eluted by heating at 100°C for 5 min in Laemmli sample loading buffer (Bio-Rad, USA), and resolved on an SDS/PAGE ...
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bioRxiv - Plant Biology 2024Quote: ... prior to bombardment with 10 mg 1 μm tungsten beads treated with 5 μg purified plasmid DNA in a Helios gene gun (BioRad) at 1,550 PSI ...
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Real time quantitative PCR (qRT-PCR) was performed using an iQ™5 Multicolor Real-Time PCR Detection System (BIO-RAD) with qPCRBIO SyGreen Mix fluorescein (PCR BIOSYSTEMS) ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The blots were washed four times with TBST for 5 minutes each before developing with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging ...
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bioRxiv - Immunology 2021Quote: ... They were incubated at the room temperature for 5-10 min and they were placed inside a 4-mm cuvette (Bio-Rad). The cuvettes were pulsed using the BTX electroporator (300 V ...
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bioRxiv - Genetics 2019Quote: ... and heated at 90°C for 5 min before being run on a stain free gel and imaged by GelDoc imager (BIO-RAD).
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 5 minutes at 95°C before separation on a gradient 4-20% SDS-PAGE gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semi-dry transfer (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were washed three times with TBST for 5 minutes and incubated with Goat Anti-Rabbit IgG (Bio-Rad, 170-6515) for 1 hour at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 seconds at 72°C and a final extension of 5 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). In order to standardize the expression pattern of NvDsx ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were then diluted 1:10 and 2 µL of diluted cDNA were used for RT-qPCR along with 5 µL of iTaq universal SYBR mix (Bio-Rad), 1.5 µL of primers (1.5 µM mix of forward and reverse ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...