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Citations for Bio-Rad :
1051 - 1100 of 1641 citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... was added to a final concentration of 1X to the quenched reaction and samples were loaded onto a 5% TBE gel and run for 60 min at 150 volts in 0.5X TBE Buffer (BioRad). Gels were stained with ethidium bromide and imaged on a BioRad ChemiDoc ...
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bioRxiv - Immunology 2022Quote: ... Internal loading control was performed by stain free visualization after 5 min activation on a ChemiDoc imaging system (BioRad). Protein transfer was done either on nitrocellulose membrane or polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2022Quote: ... 5% β-mercaptoethanol) to be run on SDS-PAGE gels using the Mini Protean system (Bio-Rad, no. 1658004). Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 minute and then imaging every 10 seconds for 5 minutes on the ChemiDoc XRS+ machine (Bio-Rad). ImageLab (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using 5% non-fat milk in 0.1% TBS-T (1X Tris-Buffered Saline, 0.1% Tween 20, Bio-Rad) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and then boiled for 5 minutes before being loaded into either a precast 10% polyacrylamide gel (4561034, Bio-Rad) or manually made gel for electrophoresis with Tris-glycine-SDS running buffer (24.8 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... samples were loaded onto Mini-PROTEAN TGX Pre-cast Stain-Free gels (5-15%, BioRad Laboratories, Hercules, CA, USA) and total protein was visualised post-transfer to PVDF membranes on ChemiDoc Touch Imaging System ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µL of the supernatant was loaded into a 4−20 % Mini-PROTEAN-TGX gel (BioRad, Hercules, CA, USA), run at 165 V for 50 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Each reaction consisted of 5 μl of extracted DNA and 15 μL of iTaq™ Universal Probes Supermix (BioRad), containing 0.4 mM of each primer ...
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bioRxiv - Microbiology 2023Quote: ... both proteins were supplemented with 5 mM MgCl2 and protein concentration was determined using a colorimetric assay (Bio-Rad). Proteins were then mixed at a 2.5:1 mass ratio of KtrC to N-terminal Strep-tagged KtrA (11 mg of KtrC and 4.5 mg of N-terminal Strep-tagged KtrA in 10 mL total volume of 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.5] with 0.1% (v/v) Tween 20) containing 5% Blotting-Grade Blocker (Bio-Rad Laboratories, cat. #170-6404) for 30 mins ...
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bioRxiv - Genetics 2023Quote: ... The membrane was blocked 1 hr with 5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad, Cat# 1706404XTU) and incubated overnight with the primary antibodies [TTC3 Polyclonal Antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf lysates were cleared by centrifugation at 10,000×g for 5 min and total protein normalized via Bradford Assay (Biorad). Extracts were prepared for SDS-PAGE with a 5x loading buffer containing 10% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... To visualize the protein bands, membranes were incubated (5 min, RT) in Clarity™ western ECL substrate (Bio-Rad) and imaged using the ChemiDoc™ MP imaging system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... The transferred membrane was briefly washed with TBST (0.1% Tween-20 in TBS) and blocked with Blocking Buffer (5% Blotting-Grade Blocker [1706404, BioRad] or 5% BSA [A9647-50G ...
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bioRxiv - Immunology 2023Quote: ... cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primer ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg/sample was loaded onto 4-15% gradient polyacrylamide gels (Mini-PROTEAN TGX Gels, Bio-Rad, 4561083), transferred (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... and 20 μl were mixed with 5 μl 5x Lämmli buffer for loading on a 10% SDS-PAGE gel (BioRad). After blotting was done on nitrocellulose using the semi-dry system (BioRad) ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was diluted 1:5 and RT-qPCR was done using SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad) with PrimePCR Assays (CCDC40 ...
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bioRxiv - Plant Biology 2023Quote: ... at 1:10,000 dilution in 5% milk PBST and ECL prime chemiluminescent reagent (Amersham: RPN2236) on a ChemiDoc gel imager (Biorad).
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bioRxiv - Physiology 2023Quote: ... Total protein (5∼15 µg) was loaded and separated by 4–20% pre-made SDS-PAGE gels (#4568095, BioRad, Mini-PROTEAN TGX Stain-Free Precast Gels ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein samples (5-20µg) were diluted in RIPA buffer and βME-Laemmli buffer (Bio-Rad Laboratories) to equal volumes and boiled for 7 minutes at 95°C ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was loaded onto a 5 mL Bio-Scale Mini Nuvia IMAC Ni-charged Cartridges (Bio-Rad) which was pre-equilibrated with 5 column volumes (CV ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Molecular Biology 2023Quote: Quantitative PCR (qPCR) was performed in 10 µL reactions containing 5 µL SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µL H2O ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were heated to 95°C with 2X sample buffer for 5 mins before loading the samples directly to a 0.2μm nitrocellulose (BioRad, #1704159) membrane ...
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bioRxiv - Microbiology 2024Quote: ... and then lysed by a 5-minute incubation with with 50 uL iScript RT-qPCR Sample Preparation Reagent (BioRad) (64) ...
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: 0.2 – 1 mL (sufficient to cover the sample) of 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA, USA) with 0.2% Tween20 and 0.1 mg/mL proteinase K (Merck ...
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bioRxiv - Molecular Biology 2024Quote: Input lysates (5 μg of total protein) and entire elution products were combined with 4x Laemmli buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... After 1h-incubation with HRP-conjugated secondary antibodies diluted in 5% milk in TBST (anti-mouse HRP (BioRad, 1706516) 1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked with 5% non-fat milk in PBS-T (0.5% Tween-20 [1706531, Bio-Rad] in PBS) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were loaded onto polyacrylamide native gels (5% polyacrylamide 29:1 [BioRad], 1× TBE, 0.3% APS, and 0.06% Temed) and protein/DNA complexes were resolved by electrophoresis at 60 V for 2 h at 24 °C in 1X TBE ...
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bioRxiv - Biophysics 2024Quote: ... Blots were washed 3 times with TBST for 5 min and visualized using a ChemiDoc MP Imaging System (Biorad).
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bioRxiv - Biochemistry 2024Quote: ... The enzyme was purified using a 5-mL Ni-charged EconoFit Nuvia IMAC Column (Bio-Rad, Hercules, CA, USA) and a linear gradient of 10 - 250 mM imidazole in binding buffer (100 mL in total at 2.5 mL/min flow rate) ...
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bioRxiv - Cell Biology 2024Quote: ... and heated to 98°C for 5 min and transferred to Trans-Blot PVDF Transfer membranes (Bio-Rad, #1704157). Membranes were blocked for 1 h at room temperature with TBS-T (0.1% Tween 20 ...
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bioRxiv - Cell Biology 2024Quote: ... rinsed twice with water and resuspended in 20 µL Laemmli sample buffer (Bio-Rad, 2X with 5% β-mercaptoethanol). After boiling for 5 min at 100°C ...
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bioRxiv - Biophysics 2020Quote: The prepared DNA samples (5 μL) were mixed thoroughly with 1 μL of 6X DNA loading buffer (Bio-Rad Laboratories). The mixtures were loaded into the wells of the prepared gel ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified mouse α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Cell Biology 2019Quote: Polymerization of the soft pre-mix was started immediately after the previous step by addition of 5 µl 10% ammonium persulphate (APS; BioRad) and 1 µl N ...
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bioRxiv - Microbiology 2021Quote: ... and switch time linearly ramped from 1 s to 5 s over 14-15 h (CHEF DR III, Bio-Rad). DNA bands were visualized by staining the gel with a solution of 0.5 µg/mL ethidium bromide ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’KI and 3’KI as detailed (Fig 2A and D) with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were boiled at 95 °C for 5 mins with 6xSDS sample buffer before loading onto 4-20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... by Bio-Rad tans-blot turbo transfer system at mixed weight transfer setting and incubated in 5% blotting grade blocker (BioRad Catalog: 1706404) solution for 1 hour ...
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bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was obtained by incubating the samples (whole embryos or adult caudal fin fragments) in TE buffer supplemented with 5% Chelex-100 (BioRad) and 10 μg/ml Proteinase K (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... Tween 20 (TBS-T) in 5% (w/v) milk (pH 7.4) followed by incubation with an anti 6xhistidine antibody (Bio-Rad) overnight ...
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bioRxiv - Neuroscience 2021Quote: ... A 1:5 dilution from serum samples was combined with 4 °C Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories ...