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Citations for Bio-Rad :
1201 - 1250 of 7379 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 µg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
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bioRxiv - Neuroscience 2023Quote: ... and the supernatants were incubated with either 2 µg of anti-PAR antibody (clone #19, custom designed at AbD Serotec, Bio-Rad) or Human IgG F(ab)2 (Rockland ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription reaction was conducted using 0.5 µg of total RNA and 2 µl of iScript™ Reverse Transcription Supermix (Bio-Rad) in 10 µl reaction volume ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Genomics 2024Quote: ... Samples were then run on a 2% agarose gel with 1x SYBR Safe for 2 hours at 120 V before gel extraction with a Freeze ‘N Squeeze column (Bio-Rad). After extraction ...
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bioRxiv - Biochemistry 2023Quote: ... This mixture was then incubated at 4°C for 2 h with gentle agitation before reconstitution was initiated by removing detergent by incubating with Bio-beads (Bio-Rad) at 4°C overnight with constant rotation ...
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bioRxiv - Cancer Biology 2023Quote: ... before solubilisation in 2% SDS (Severn Biotech, Worcs, UK) and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad). Data were normalised by dividing raw OCR/ECAR measurement by OD595 for each well of the culture plate ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747, BioRad, Hercules, CA, USA) and run on a Mini-PROTEAN® TGX™ Precast Gel (#4561083 ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... before solubilisation in 2% SDS (Severn Biotech, Worcs, UK) and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad). Data are expressed as OD595 of selected timepoint relative to OD595 at day 0.
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bioRxiv - Cancer Biology 2022Quote: ... and 2 μg of RNA was used to synthesize cDNA with Bio-RAD iScript cDNA Synthesis Kit (170-8891, Bio-Rad). qRT-PCR was performed using Taqman universal PCR mix and predesigned gene expression assays with best coverage from Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins (10μl solubilized in 2X Laemmli/20% of 2-mercaptoethanol) were separated by SDS-PAGE on 10% polyacrylamide gel (Bio-Rad, Hercules). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The mixture was transferred to a pre-chilled 2 mm gap electroporator cuvette and cells were electroporated using the EC2 program (MicroPulser, Bio-Rad). Cells were then suspended in 3 ml 1/4 TSB (at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Three more washing steps of 2% w/v TBST/milk were applied and finally the membranes were screened with a ChemiDoc MP Imaging system (Biorad, USA) and freshly mixed ECL fluorescent mixture and optimal exposure times ...
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bioRxiv - Immunology 2022Quote: ... were coated with 50 µL/well of 2 µg/mL of mouse anti-bovine IFN-γ mAb (CC330 Bio-Rad Antibodies) diluted in carbonate coating buffer (pH 9.6 ...
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...
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bioRxiv - Microbiology 2022Quote: Cell pellets from 2 mL overnight cultures were used to create gel plugs using the CHEF Bacterial Genomic DNA Plug Kit (Bio-rad). DNA was digested with I-SceI ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We then performed quantitative polymerase chain reaction (qPCR) using 2 ng of complementary DNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... The pellets were resuspended with 2 % SDS and loaded onto a cellulose acetate membrane assembled in a slot blot apparatus (Bio-Rad). The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... tabacum) BY-2 suspension-cultured cells and biolistic bombardment using a PDS-1000/He biolistic particle delivery system (Bio-Rad Laboratories) were performed as previously described (Banjoko and Trelease ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Genomics 2023Quote: ... 10ul of PCR product of each replicate for each gene was loaded in separate wells in 2% agarose gels and imaged using a ChemiDocTM MP Imaging system (BioRad, USA). All replicates for each gene were loaded on the same gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of RNA template was converted to cDNA using iScript™ cDNA Synthesis Kit (1708891, Bio-Rad, Hercules, CA, USA), according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Genetics 2024Quote: ... Membranes were were in contact with the luminol-H2O2 solution for 2 minutes and then exposed for imaging using the Chemidoc imaging system (Bio-Rad).
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bioRxiv - Biophysics 2024Quote: ... then diluted below the CMC of C12E8 with reconstitution buffer and added to 0.5 g methanol-activated Biobeads SM-2 (Bio-Rad; 1523920). The mixture was rotated end over end for 18 hours and then subject to room temperature size exclusion chromatography on a Superose 6 10/300 GL column ...
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bioRxiv - Microbiology 2024Quote: ... 10 µg Capped mRNA transcripts were electroporated into BHK-21 cells in a 0.2 cm cuvette (25 μF, 1.5 kV, and 200 Ω) with the help of Gene Pulser Xcell Electroporation system (Bio-Rad) and resuspended in antibiotic-free DMEM supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed using 2 uL of diluted cDNA in a 20-µL reaction with SYBR Green dye polymerase mix (SsoAdvanced; Bio-Rad) in white-walled plates in a CFX96 thermocycler (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... 500 μL of protoplasts in a total volume of 600 μL electrotransfection buffer were electrotransfected with 2-6 μg plasmid DNA using the Gene Pulser XcellTM (Bio-Rad) with a pulse at 160 V for 10 ms ...
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bioRxiv - Immunology 2024Quote: ... for two hours at RT with mild rotation and proteins in FhNEJ-Som were acetone-precipitated using the ReadyPrep 2-D Cleanup kit (BioRad, #1632130) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... detergents in FhNEJ-Teg and FhNEJ-Som were removed as previously described 46: FhNEJ-Teg was incubated with SM-2 Adsorbent Bio-Beads (BioRad, #1523920) for two hours at RT with mild rotation and proteins in FhNEJ-Som were acetone-precipitated using the ReadyPrep 2-D Cleanup kit (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... Samples were mixed in nanodisc buffer (25 mM Napi, 100 mM NaCl, pH 7.0) with Bio-beads™ SM-2 Adsorbent Media (Bio-RAD) for cholate and detergent (DDM ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed in PBS-T and developed for 2 min with ClarityTM Western ECL Substrate (Bio-Rad, CA, United States). Imaging was performed using a ChemiDocTM MP Imaging System with Image LabTM software (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... This solution was diluted 4-fold then placed in 3 mL Slide-a-lyzer 10 kDa MWCO cassette (Pierce) and dialyzed against 250 mL of SUV buffer in the presence of 2 g SM2-biobeads (Bio-Rad) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and 70 mM 2-mercaptoethanol) and set on the glucose oxidase enzymatic system (GLOX) for STORM using a frame-sealed incubation chamber (Bio-Rad). 2D-STORM image acquisitions were performed using a Nikon N-STORM with Eclipse Ti-E inverted microscope and laser TIRF illuminator (Nikon Instruments Inc.) ...
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bioRxiv - Cancer Biology 2024Quote: ... Relative mRNA expression was quantified using the 2-ΔΔCT method [95] after obtaining CT values from the CFX Manager software (Bio-Rad). Primer sequences are provided in Supplementary Table 6.
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bioRxiv - Biochemistry 2024Quote: ... The gels were dried at 56 °C for 2 hours in a BioRad Model 583 gel dryer (BioRad Cat # 165-1745), rehydrated and subjected to southern blotting using 5’-32P labeled oligonucleotide probes targeting either the extrusion containing strand (Fwd 1947 ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR was performed with TaqMan Fast Advance Mastermix and primer pairs listed in Supplementary Table 2 and analysed using a CFX96TM Real-Time PCR System (Bio-RAD). Gene expression was normalised to glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA (2 μg) was subjected to cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). Real-time PCR was performed on an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... transferred to a nitrocellulose membrane (Cytiva) for 2 h (at 30V) and detected using infrared light on a Chemidoc MP (Bio-Rad).
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bioRxiv - Genetics 2024Quote: ... 2 µL of the diluted cDNA samples are supplemented with 10 µL of Supermix 2X ddPCR (without dUTP, Bio-Rad, #1863024), 1 μl of target probe (ZEN™ FAM)/primers mix (final concentration of 750 nM of each primer and 250 nM of probe ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA was amplified using the primers listed in Extended Table 2 and the SsoAdvanced universal SYBR Green supermix (Bio-Rad). Amplimers for vacA ...
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bioRxiv - Genetics 2021Quote: ... DNA was then diluted 1:100 and 1:10 and combined with QX200 ddPCR EvaGreen Supermix (BioRad) and custom-designed primers targeting either Slx/Slxl1 ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was diluted between 1:10 and 1:20 for qPCR experiments and SsoFast EvaGreen Mastermix (Biorad) was used to amplify cDNA ...
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bioRxiv - Bioengineering 2020Quote: ... Blots were incubated in ECL reagents (1:1 mix of luminol/HRP substrate solutions; Bio-Rad # 1705062) for 1 min and then imaged for 120 s using the ChemiDoc imaging system (Bio-Rad # 17001401) ...
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bioRxiv - Bioengineering 2020Quote: ... Hydrogels were incubated in ECL reagent (1:1 mix of luminol/HRP substrate solutions; Bio-Rad # 1705062) for 1 min and then imaged for 300 s using the ChemiDoc imaging system (Bio-Rad # 17001401) ...
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bioRxiv - Cell Biology 2020Quote: ... HRP secondary antibodies were used as follows: 1:500 to 1:1000 anti-mouse (BioRad, 170–6516) and 1:500 to 1:1000 anti-rabbit (BioRad ...
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bioRxiv - Genetics 2022Quote: ... the membrane was incubated in a 1:1 mixture of Clarity Western ECL Substrate reagents (Bio-Rad). Blots were then imaged on a ChemiDoc MP Imaging System and analyzed with Bio-Rad Image Lab software (v6.1.0 build 7) ...