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Citations for Bio-Rad :
1451 - 1500 of 7379 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The assembled products were mixed with electrocompetent cells and transformed into electrocompetent cells in a 2-mm Gene Pulser cuvette (Bio-Rad, 165-2086) using an electroporation instrument (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Twenty µg (islet sample) or 2 µg (mouse liver sample: positive control) was mixed with 10uL of Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and denatured at 95°C for 10 min ...
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bioRxiv - Cell Biology 2023Quote: RT-PCR was performed after generating first-strand cDNAs from 2 µg of total RNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA), as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... plates were stained with primuline (0.05% in acetone/water, 8/2; v/v) and photographed under long-wave UV light (Biorad ChemiDoc XRS Imaging System).
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (0.2 µm pore size) using the Trans-Blot® Turbo™ semi-dry transfer system (7 min mixed MW MIDI program) (Bio-Rad). After blocking in a 2.5% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: mMEC and hMEC samples were directly homogenized using 2x Laemmli Sample Buffer and supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Proteins were separated by electrophoresis on a polyacrylamide gradient gel (BioRad Hercules ...
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bioRxiv - Immunology 2023Quote: ... The amplified products were analyzed on a 2% agarose gel stained with SYBR safe and imaged using the Gel-documentation system (Bio-Rad, Hercules, CA).
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bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... and loaded (crude homogenate: 10 µg per lane; membrane: 2 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2024Quote: ... Ten μg (final volume 2 μl for all samples) of serum proteins were loaded onto a nitrocellulose membrane (Bio-rad Laboratories, CA, USA). Then ...
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bioRxiv - Cell Biology 2024Quote: ... PCR enriched fragments were further size-selected by running the PCR reaction mix in 2% low-molecular weight agarose gel (Bio-Rad, 161-3107) and subsequently purifying them using QIAquick Gel Extraction Kit (28704) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cDNA was amplified by qPCR with specific primers (Supplementary Table 2) using the C1000™ thermal cycler with CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories). The relative expression of genes was analyzed quantitatively by the ΔΔCt method ...
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bioRxiv - Microbiology 2024Quote: ... Target bands were visualized by 2% agarose gel electrophoresis with GelRed staining and recorded by a gel imager (GelDoc; Bio-Rad, California, U.S.).
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bioRxiv - Cell Biology 2024Quote: ... PCR enriched fragments were further size-selected by running the PCR reaction mix in 2% low-molecular weight agarose gel (Bio-Rad, 161-3107) and subsequently purifying them using QIAquick Gel Extraction Kit (28704) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were resolved on 1× TAE 1% agarose gels and imaged using Gel Doc EZ System (Bio-Rad). Raw gel scans are provided in SI Data File 4 ...
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bioRxiv - Cell Biology 2021Quote: ... HRP (horseradish peroxidase)-conjugated goat anti-mouse (#176516, 1:10,000 for WB) and anti-rabbit IgG antibodies (#176515, 1:10,000 for WB) were from Bio-Rad. Alexa Fluor conjugated goat anti-mouse (1:500 for IF) ...
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bioRxiv - Microbiology 2020Quote: ... membranes were blocked overnight in PBST supplemented with 5% non-fat dry milk and incubated with antiserum (1:5,000) or vaginal washes (1:500) from test or control mice followed by goat anti-mouse IgG (BioRad) or IgA (SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1 h at room temperature with peroxidase-conjugated anti-mouse (1:3000; BioRad, Hercules, CA) or anti-rabbit (1:3000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected with anti-rabbit (1:3,000) and anti-mouse (1:20,000) secondary antibodies coupled to horseradish peroxidase (Bio-Rad), diluted in 3% BSA-PBST ...
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bioRxiv - Biochemistry 2022Quote: ... the responses obtained from the AmQNS-small molecular interactions at different concentrations were fitted using the Langmuir 1:1 biomolecular interaction model using the ProteOn Manager software version 3.1.0.6 (Bio-Rad, USA). Equilibrium dissociation constants (KD ...
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bioRxiv - Microbiology 2020Quote: One ug of each respective protein was mixed at a 1:1 ratio with 2X Laemmli buffer (Bio-Rad) which was supplemented with 2% β-mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... or by anti-Smc3 K112/K113 Acetylation (1:1,000, gift from Dr. Katsuhiko Shirahige) in combination with goat anti-mouse HRP (1:10,000) (Bio-Rad) and ECL prime (GE Healthcare ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged to pellet tissue debris and equal volumes mixed 1:1 with 2x Laemmli sample buffer (BioRad). Samples were boiled ...
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bioRxiv - Biochemistry 2021Quote: ... was performed with 1:1,000 or 1:100,000 diluted PCR1 product using SsoAdvanced Universal SYBR green supermix (Bio-Rad). To quantify the Pten 5-6k isoform ...
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bioRxiv - Microbiology 2022Quote: ... GroEL was detected with anti-GroEL primary antibody (Pierce; 1:10,000) and anti-rabbit secondary antibody (Biorad; 1:20,000).
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bioRxiv - Biochemistry 2024Quote: ... Samples were loaded onto polyacrylamide native gels (5% polyacrylamide 29:1 [BioRad], 1× TBE, 0.3% APS, and 0.06% Temed) and protein/DNA complexes were resolved by electrophoresis at 60 V for 2 h at 24 °C in 1X TBE ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Bioengineering 2023Quote: ... A 10 μL aliquot of each assembly reaction was mixed 1:1 with Native Sample Buffer (Bio-Rad Laboratories), loaded into precast 4-15% polyacrylamide gels (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2023Quote: PFA fixed brain samples were incubated in 1% hydrogel monomers (HMs) consisting of 1% w/v acrylamide (#1610140, BioRad), 0.05% w/v bisacrylamide (#1610142 ...
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bioRxiv - Genomics 2022Quote: Antibodies and their working concentrations: anti-mouse (1:5000; #7076, Cell Signaling Technology, or 1:20000, #STAR117D800GA, Bio-Rad), anti-rabbit (1:5000 ...
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bioRxiv - Biochemistry 2023Quote: ... The nitrocellulose membrane was exposed to a 1:1 v/v solution of Clarity Western ECL Substrate (Bio-rad) for 5 mins and chemiluminescence quantified and analysed using FluorChem E software (ProteinSimple).
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed three times with TBST and then incubated for 60 min at room temperature with horseradish peroxidase-conjugated secondary antibodies (1:3,000 dilution, ref: 172-1011 and 1:5,000 dilution, ref: 170-6515, Bio-Rad) in blocking buffer ...
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bioRxiv - Bioengineering 2024Quote: ... as a primary antibody and goat anti-rabbit IgG HRP conjugate (1:10,000 dilution in 1% milk, Bio-Rad) as a secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated anti-rabbit (1:10,000) or anti-mouse (1:10,000) antibodies and a Western ECL Substrate kit (Bio-Rad) were used for signal detection.
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bioRxiv - Biophysics 2024Quote: ... and bound antibody was detected with chemiluminescence using HRP substrate and luminol in a 1:1 ratio (Bio-Rad). Protein band intensities were measured via densitometric analysis of Western blot data using the Image Lab software from Bio-Rad.
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 dilution) followed by Goat anti-mouse IgG (H+L) HRP conjugate at 1:1000 (Biorad 172-1011). The number of infected cells were revealed using True Blue™ peroxidase substrate (KPL 50-78-02 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were rinsed twice in TBS with 0.1% Tween and incubated for 1 h with secondary antibodies coupled to a horseradish peroxidase (HRP) (1/3000; BioRad) at RT ...
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bioRxiv - Bioengineering 2024Quote: ... and interleukin 4 (IL-4)) cytokines run on a Bio-Plex 200 reader (BioRad). Data was collected from only two donors for RANTES ...
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bioRxiv - Molecular Biology 2021Quote: ... 4–20% gel (Bio-Rad) using SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... 0.8 × 4 cm (Bio-Rad). The column was washed with 10 column volumes (c.v. ...
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bioRxiv - Cell Biology 2020Quote: ... 4-20% TGX gels (BioRad) were used ...
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bioRxiv - Cell Biology 2021Quote: ... 4-20% gradient (Bio-Rad,); and then proteins were transferred to PVDF membranes ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-15% (Bio-Rad #4561085), with 1X Tris/Glycine/SDS Buffer (Bio-Rad #1610732) ...
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bioRxiv - Cancer Biology 2024Quote: ... 4-12% acrylamide gels (BioRad) were loaded with protein samples ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified mouse α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified goat α-rabbit or goat α-mouse IgG conjugated to alkaline phosphatase ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated secondary antibodies were diluted 1:10000 and 1:2000 for polyclonal Goat-α-Mouse (Bio-Rad, 170-6516) and Goat-α-Rabbit HRP (Dako ...
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bioRxiv - Genomics 2021Quote: ... 1 mM EDTA at pH 8.3) and by pulse field gel electrophoresis in 1% Certified™ Megabase Agarose (Bio-Rad) in 1X TAE buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were next washed and incubated for 1 hour at room temperature with peroxidase conjugated anti-rabbit (1:5000, BioRad), or anti-mouse (1:5000 ...