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Citations for Bio-Rad :
1151 - 1200 of 1388 citations for 6 PHOSPHOFRUCTO 2 KINASE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... Reaction mixtures were incubated for 2 hours at 25 °C and quenched by adding 10 μL 4x Laemmli Sample buffer (Bio-Rad). Samples were boiled at 95 °C for 5 min and each 8 μL were loaded onto 12% SDS-PAGE gels ...
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bioRxiv - Plant Biology 2022Quote: ... 500 nM concentration of each PCR primer and 2 μL of 15x diluted cDNA templates using CFX96 real-time PCR system (Bio-Rad) as described (Gayomba et al. ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The reactions were assembled isothermally on a PTC-225 Peltier Thermal Cycler (MJ Research) or a Tetrad 2 Peltier thermal cycler (Bio-Rad) at a suitable growth temperature (e.g ...
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bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions or ISVPs (2×1012 particles/ml) were incubated for 5 min at the indicated temperatures in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Developmental Biology 2019Quote: ... total RNA from Astyanax embryos of various stages (2-24 hpf) was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad) and amplified using the following primers:
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bioRxiv - Zoology 2020Quote: ... Samples were incubated in a 1:1 (w/v) ratio of 2× sample buffer (0.2M DTT, BioRad Laemmli Sample Buffer #1610737) at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of each protein sample was denatured and reduced by boiling at 95°C for 5 min in 10% (v/v) 2-mercaptoethanol-containing Laemmli sample buffer (Bio-Rad). Samples were resolved by SDS-PAGE with a precast Tris-glycine gel (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: ... to nitrocellulose membranes using a constant voltage of 100 V for 2 h using the BioRad Midi PROTEAN Western blotting module according to the manufacturer’s instructions (BioRad, CA, USA). The membranes were blocked with blocking solution [Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads were washed five times with IP lysis buffer and were boiled for 5-10 min in 2 times Laemmli sample buffer (#1610737, Bio-Rad) with 2-mercaptoethanol (#BP176-100 ...
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bioRxiv - Genomics 2021Quote: The nuclei solution were adjusted to 43°C for 3 min and melted 2% agarose from CHEF Genomic DNA Plug Kits (Bio-Rad) was added to reach a 0.82% agarose plug concentration ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was incubated at 37°C for 30 minutes then left to cool for 10 minutes before adding 6 μL of 0.2% 2-mercaptoethanol in SDS-page loading dye.38 Samples were then heated to 94°C for 2 minutes and added to the wells of a pre-cast 12% polyacrylamide gel (Bio-Rad). The gel was run for 45 minutes at 120 V in running buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was transformed into Escherichia coli SS320 cells by electroporation in a 2-mm cuvette using a Gene Pulser Xcell (Bio-Rad) with a setting of 2,400 volts ...
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bioRxiv - Biochemistry 2021Quote: ... Proteoliposomes or protein-free liposomes were generated by two rounds of treatment with 300 mg of wet SM-2 Bio-Beads (Bio-Rad) in total ...
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bioRxiv - Neuroscience 2021Quote: ... In a water bath samples were boiled for 2 minutes and 12 ul of each lysate were loaded on two separate gels (Biorad, #4568096), one each required for parallel HTT and pS13 HTT detection ...
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bioRxiv - Microbiology 2020Quote: ... Optimized ddPCR was used to detect the presence of SARS-CoV-2 viruses following our previous study.10 Analysis of the ddPCR data was performed with QuantaSoft software (Bio-Rad). The concentration reported by the procedure equals copies of template per microliter of the final 1x ddPCR reaction ...
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bioRxiv - Immunology 2020Quote: ... in reducing or nonreducing conditions using SuperSep Ace 5%–20% gradient polyacrylamide gel (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 2 × Laemmli Sample Buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... the primer pairs (Table 2; exception: S. umbla) were used to create EvaGreen-based droplet dPCR assays using the AutoDG (Bio-Rad) for droplet generation ...
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... The Ct value was determined by CFX Maestro software when the fluorescence rises exponentially 2-fold above background (Biorad, Hercules, CA.). The reference group was the negative control birds.
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bioRxiv - Neuroscience 2021Quote: ... Gels were run for 2 hours and 10 min at 80V and transferred using the Trans-Blot SD Semi-Dry Transfer cell system (Bio-Rad). After transfer the LI-COR Revert 700 Total Protein Stain (LI-COR ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added to the control wells without cells (Lanes 1 and 12) ranging from 0.125 – 2 mg/ml (BioRad 500-0202) and treated with formalin and NaOH at the same concentrations as the cells ...
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bioRxiv - Molecular Biology 2020Quote: ... or in transfer buffer (25 mM Tris, 200 mM glycine, 20% ethanol) for 2 h at 52V with a liquid transfer system (Bio-Rad). Total proteins were revealed by Pierce® Reversible Stain (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... and by linear regression in log10 copies/ml with a standard curve realized from a SARS-CoV-2 positive nasopharyngeal sample quantified by Droplet-Digital PCR (Bio-Rad). IC50s were analyzed by nonlinear regression using a four-parameter dosage-response variable slope model with the GraphPad Prism 8.0.2 software (GraphPad Software ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 μg of RNA was used to synthesize cDNA with Bio-RAD iScript cDNA Synthesis Kit (170-8891, Bio-Rad). qRT-PCR was performed using Taqman universal PCR mix and predesigned gene expression assays with best coverage from Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins (10μl solubilized in 2X Laemmli/20% of 2-mercaptoethanol) were separated by SDS-PAGE on 10% polyacrylamide gel (Bio-Rad, Hercules). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The mixture was transferred to a pre-chilled 2 mm gap electroporator cuvette and cells were electroporated using the EC2 program (MicroPulser, Bio-Rad). Cells were then suspended in 3 ml 1/4 TSB (at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Three more washing steps of 2% w/v TBST/milk were applied and finally the membranes were screened with a ChemiDoc MP Imaging system (Biorad, USA) and freshly mixed ECL fluorescent mixture and optimal exposure times ...
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bioRxiv - Immunology 2022Quote: ... were coated with 50 µL/well of 2 µg/mL of mouse anti-bovine IFN-γ mAb (CC330 Bio-Rad Antibodies) diluted in carbonate coating buffer (pH 9.6 ...
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Microbiology 2022Quote: Cell pellets from 2 mL overnight cultures were used to create gel plugs using the CHEF Bacterial Genomic DNA Plug Kit (Bio-rad). DNA was digested with I-SceI ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We then performed quantitative polymerase chain reaction (qPCR) using 2 ng of complementary DNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... The pellets were resuspended with 2 % SDS and loaded onto a cellulose acetate membrane assembled in a slot blot apparatus (Bio-Rad). The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... tabacum) BY-2 suspension-cultured cells and biolistic bombardment using a PDS-1000/He biolistic particle delivery system (Bio-Rad Laboratories) were performed as previously described (Banjoko and Trelease ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral copy numbers were determined using SARS-CoV-2 RdRp gene specific primers and probe (see [28]) and droplet digital PCR (Bio-Rad Laboratories Inc. ...
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bioRxiv - Immunology 2022Quote: Serum samples were obtained from blood collected from the submandibular vein at the indicated timepoints and incubated on Kallestad HEp-2 slides (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were prepared for loading by mixing 30 μg of protein in 5xSDS sample buffer containing 15% 2-Mercaptoethanol (Bio-Rad) and heated at 90 °C for 5 minutes ...
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bioRxiv - Physiology 2022Quote: ... followed by a final melting curve analysis from 70°C to 95°C with 0.2°C increment for each step using the CFX96 Real-Time PCR System and the Precision Melt Analysis software (Bio-Rad). For HMA analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% of total input and 15% of total IP fraction were resolved on 10% SDS-PAGE gels and wet transferred onto a 0.45 µm nitrocellulose membrane for 2 h at 80 V and 4°C (Bio-Rad, Germany). The membrane was blocked for 1 h in 5% BSA in PBST (PBS + 0.2% Tween20) ...
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then transferred to a PVDF membrane at 400mA for 2 hours at 4°C in 1x Tris/Glycine buffer (1610771, Bio-Rad). Membranes were then blocked in casein blocking buffer (PI37528 ...
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bioRxiv - Genomics 2024Quote: ... 10ul of PCR product of each replicate for each gene was loaded in separate wells in 2% agarose gels and imaged using a ChemiDocTM MP Imaging system (BioRad, USA). All replicates for each gene were loaded on the same gel ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR was performed with the 2× S6 SYBR Premix EsTaq plus (Scintol) on the CFX Connect Real-Time System (Bio-Rad). The data were analysed using the 2-ΔΔCt method ...
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bioRxiv - Genomics 2024Quote: ... This cDNA was then run on a 2% agarose gel in TAE buffer alongside a 100bp-1kb DNA ladder (Bio-Rad). After electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 µg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
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bioRxiv - Cell Biology 2023Quote: ... followed by washes at room temperature (1 x 15 min, 2 x 5 min) and incubation with HRP-conjugated secondary antibodies (Bio-Rad) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... and the supernatants were incubated with either 2 µg of anti-PAR antibody (clone #19, custom designed at AbD Serotec, Bio-Rad) or Human IgG F(ab)2 (Rockland ...
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bioRxiv - Cancer Biology 2023Quote: ... and the fold change was calculated using the 2−ΔΔCT method using Bio-Rad CFX384 Touch Real-Time PCR System and software (Bio-Rad). The results were expressed either as the relative log2 FC (fold change ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction products were visualized on a 2 % agarose gel with 0.05% (v/v) Ethidium Bromide and visualized with a Gel Doc XR+ imager (Bio-Rad Laboratories). PCR amplicons were column purified with the Monarch® PCR & DNA Cleanup Kit and eluted in 25 µL of Monarch® DNA Elution Buffer.