Labshake search
Citations for Bio-Rad :
1001 - 1050 of 1388 citations for 6 PHOSPHOFRUCTO 2 KINASE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... The input (15 µg) and eluted proteins (∼2% of IPT) were fractionated in 4−15 % SDS-PAGE gels (Bio-rad) and analyzed by immunoblotting ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing 2 μl of five times diluted cDNA and monitored by CFX Connect Real-Time PCR Detection System (Bio-Rad). The following cycling parameters were used ...
-
bioRxiv - Microbiology 2020Quote: ... at 1/10,000 dilution in TBE buffer for 2 h with rocking and visualised using a Gel Doc EZ Imager (Bio-Rad). Band intensity was quantified using ImageJ software ...
-
bioRxiv - Plant Biology 2021Quote: ... Quantification of relative gene expression was performed using the comparative CT method (2-ΔΔCt) (Schmittgen & Livak, 2008) on a CFX96 Real-Time System (BioRad). Primers used are listed in Supplemental Table 1.
-
bioRxiv - Plant Biology 2021Quote: ... Quantification of relative gene expression was performed using the comparative CT method (2−ΔΔCt) (Schmittgen & Livak, 2008) on a CFX384 Touch Real-Time PCR Detection System (BioRad).
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed in PBS-T and developed for 2 minutes with ClarityTM Western ECL Substrate (Bio-Rad, CA, USA). Imaging was performed using a ChemiDocTM MP Imaging System with Image LabTM software (Bio-Rad ...
-
bioRxiv - Genomics 2021Quote: ... at 1.8 kV distributed over 2 cuvettes (0.1 cm gap width) per tube using a Micropulser Electroporator (Bio-Rad Laboratories). 10-fold serial dilutions of a 10 μL aliquot were plated on LB plates with ampicillin at 100 μg/mL to assess electroporation efficiency ...
-
bioRxiv - Biophysics 2020Quote: ... before being transferred to Whatman 3 MM paper and dried (50°C, 2 hours) using a gel dryer (Model 583, BioRad) attached to a vacuum pump ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 2 µl of the lysates were subject to 1 ml of 0.2x Bradford protein dye reagent (Bio-Rad #5000006) for protein quantification ...
-
bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
-
bioRxiv - Pathology 2022Quote: ... The CD68 primary antibody incubation was performed at room temperature for 2 hours (mouse CD68, BIO-RAD, MCA1957, 1:750) and CD11b staining (BD Pharmigen ...
-
bioRxiv - Neuroscience 2022Quote: ... 2 ng of ExoDNA was used to calculate mtDNA copies number with iQ™ SYBR® Green Supermix (Bio-Rad) on a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2022Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Nuclear and cytosolic fractions of iPSCs-differentiated cells were acquired using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific #78833 ...
-
bioRxiv - Microbiology 2022Quote: ... Viral copy numbers were assessed via digital-droplet PCR (ddPCR) using SARS-CoV-2 ddPCR kit (#12013743) from Bio-Rad with N1 and N2 primers and probes targeting the nucleocapsid genes ...
-
bioRxiv - Microbiology 2020Quote: ... Protoplast suspensions (2 × 108 cells/ml) containing 0.6% low melting agarose gel were added to 50-well dispensable mold plates (Bio-Rad). Plugs were immersed in 10 ml of NDS (1% N-lauroyl sarcosinate sodium salt solution ...
-
The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... Samples were run on a precasted gradient gel (4 – 15% Criterion TGX, 12+2 wells, 45 μl, BioRad, CA, USA) for 45 min at 60 mA ...
-
bioRxiv - Molecular Biology 2021Quote: ChIP- and DRIP-derived DNA samples were subjected in triplicates (1.5 - 2 µl) to quantitative real-time PCR (qPCR) analysis on a CFX96 Real-Time Analyzer (Bio-Rad) using iQ SYBR Green Supermix reagent (Bio-Rad ...
-
bioRxiv - Biochemistry 2020Quote: ... 2 µg of RNA isolated from the strain was spotted onto a Nytran membrane using a dot-blot apparatus (Biorad) and UV cross-linked (1200 energy ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from Bis-Tris gels to polyvinylidene fluoride (PVDF) membranes for 2 hours using a wet transfer system (BioRad). Following transfer ...
-
bioRxiv - Biochemistry 2021Quote: ... blots were incubated for 1-2 h at RT with an anti-rabbit HRP-conjugated secondary antibody (diluted 1 : 3,000, Bio-Rad) in TBST with 5% milk ...
-
bioRxiv - Genetics 2022Quote: ... The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad). Data was normalized by loading control and presented as fold change compared to control samples.
-
bioRxiv - Genetics 2022Quote: ... and a 2-μl aliquot was used in a 12-μl PCR reaction with SsoAdvanced Universal Probes Supermix (Bio-Rad) and specific TaqMan primer/probe assays for Gaa (Taqman assay #Mm00484581_m1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The Flag-M2 beads were washed 2 times with 45 ml BD450 buffer and they were transferred into a purification column (Biorad). The beads on the column were washed 2 more times with 10 ml BD450 buffer and 5 ml Elution buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2019Quote: ... cDNA (25 ng) were used for qRT-PCR using 2 x IQ SYBR Green supermix cocktail (Bio-Rad, cat. 1708880). Primers used for all genes are listed in the following Table ...
-
bioRxiv - Biochemistry 2019Quote: ... 10% of 2 mM EGTA eluates were concentrated and separated on 4%– 20% gradient SDS-polyacrylamide Tris-glycine gels (Biorad). Total protein content was visualized by silver staining ...
-
bioRxiv - Pathology 2019Quote: ... PCR products were separated electrophoretically in 2% agarose gels including 0.5 µg/ml ethidium bromide and bands were visualized in the gel-doc system (Biorad, USA). ISSR gels were photographed using FOTO Analyst Express Electronic Imaging System (Fotodyne ...
-
bioRxiv - Cancer Biology 2020Quote: ... The PCR product was then run on a 2% agarose gel and imaged with a ChemiDoc Gel Imager (Bio-Rad).
-
bioRxiv - Developmental Biology 2021Quote: ... was performed using 1 μl of cDNA as a template with qPCR primers (Table 2) and iTaq Universal SYBR Green Supermix (Biorad) according to the manufacturer’s instruction and analyzed using CFX Connect Real Time System (BioRad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Quantification of percentage of exon 2 inclusion was performed with ImageJ or with ImageLab software (version 5.2, Bio-Rad Laboratories) when 32P-labelled DUP51S_F primer was used for the PCR ...
-
bioRxiv - Cell Biology 2019Quote: ... An 11 kPa polyacrylamide (PA) solution (715 µL 50 mM HEPES pH 7.4, 150 µL 2% bis-acrylamide (Bio-Rad), 125 µL 40% acrylamide ...
-
bioRxiv - Microbiology 2021Quote: ... The nitrocellulose strips were blocked in PBS with casein for 30 mins at room temperature and then probed for 1 hour with monoclonal antibodies (mAb) recognizing H3 HA (clone 36/2 (26)) or IAV M1 matrix protein (clone MCA401, BioRad). Strips were washed and incubated for 1 hour with a horse radish peroxidase (HRP)-conjugated secondary immunoglobulin (DAKO) ...
-
bioRxiv - Developmental Biology 2019Quote: ... RT-PCR was performed with 10–100 ng of cDNA template in a 25 μl total reaction volume (12.5 μl iQ SYBR Green Supermix (2×) (Bio-Rad), 0.5 mM of each gene-specific primer ...
-
bioRxiv - Molecular Biology 2020Quote: ... the fractions that contained RTs were pooled and loaded onto a 2-ml Macro-Prep DEAE Resin (Bio-Rad, USA) column pre-equilibrated with buffer C (50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2019Quote: ... and the supernatants were purified with IBA Strep-Tactin sepharose beads (IBA, 2-1201-002) on Poly-Prep Chromatography Columns (731-1550, BioRad) with IBA’s buffer’s W (washing ...
-
bioRxiv - Cell Biology 2020Quote: ... The embryos were mounted on a 2 % agarose pad and heat-shocked at 30°C for 1h (Thermocycler Bio-Rad). After 2h recovery at 20°C ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... with modified primer lengths that allowed all six loci to be used in combination in a single PCR in a DNA Engine Tetrad 2 thermal cycler from BioRad set at the following parameters ...
-
bioRxiv - Biochemistry 2021Quote: ... 2.5×106 Jurkat T cells were electroporated with 2 μg minigene constructs using 220 V and 1,000 mA (Gene pulser X,Bio-Rad). After 24 h incubation ...
-
bioRxiv - Cancer Biology 2021Quote: Click reaction mixtures and unreacted alkyne-modified oligonucleotides were separated in a 1.2 % agarose gel for 1 h at constant 120 mV and DNA bands were captured by the ChemiDoc system (BioRad) using SYBR safe mode.
-
bioRxiv - Immunology 2021Quote: For performing the digital droplet-PCR (ddPCR) we prepared the reaction mixture by combining a 2× ddPCR Mastermix (Bio-Rad), 20× primer ...
-
bioRxiv - Biophysics 2021Quote: ... The solution was then transferred to a tube containing ∼30- 50 mg of Bio-Beads (Bio-Beads SM-2, BioRad) that were previously equilibrated in 20 mM Tris-HCl pH 7 ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR products were electrophoresed in a 2% agarose gel and visualized using a Bio-Rad gel system (Bio-Rad).
-
bioRxiv - Microbiology 2021Quote: ... Bradford Protein Assay Reagents 1 and 2 and Precision Plus Protein All Blue Prestained Protein Standard were from Bio-Rad. The anti His-tag mouse antibody was from GenScript (cat no ...
-
bioRxiv - Immunology 2020Quote: ... Cathepsin B activity was determined using Magic Red™ (a cell-permeable and non-cytotoxic reagent that contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe) and lysosomes were detected with acridine orange (Biorad), according to manufactures instructions.
-
bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
-
bioRxiv - Developmental Biology 2020Quote: ... 5 μL of PCR1 reaction (2 ng) were combined for the qPCR reaction using CFX 96 Touch Biorad qPCR thermocycler (Biorad). Δct was calculated by subtracting the mean Cq of each sgRNA to a non-targeting control ...
-
bioRxiv - Genetics 2019Quote: ... Gels were transferred in buffer containing 20% methanol and 0.03% SDS for 2 hours at 4C at 28V onto 0.2μM nitrocellulose membranes (Bio-Rad, cat #1620097). Membranes were blocked in tris-buffered saline and Tween 20 (TBST ...
-
bioRxiv - Immunology 2022Quote: ... 2 μm paraffin-embedded kidney sections were incubated with an anti-F4/80 antibody (CI:A3-1; Bio-Rad, Feldkirchen, Germany). After incubation with biotinylated goat anti-rat IgG (Abcam ...
-
bioRxiv - Biochemistry 2022Quote: ... The other two lanes were incubated with 1xTris/glycine/SDS buffer for 15 min and then incubated with 2 x Laemmli sample buffer (BioRad) for 15 min ...
-
bioRxiv - Cell Biology 2022Quote: ... and the nuclear ACTIN locus (Supplementary Table 2) were independently amplified from 12.5 ng of total genomic DNA with ssoAdvanced SYBR Green Supermix (Bio-Rad) in a total volume of 10 μl with standard cycling condition suggested by Bio-Rad ...
-
bioRxiv - Cancer Biology 2022Quote: ... before solubilisation in 2% SDS and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad, Laboratories, Inc.). The number of adherent cells was claculated by the confluence in each concentration of CB-839/UK-5099 at 72 hours ...