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Citations for Bio-Rad :
1101 - 1150 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed using 2 × T5 Fast qPCR Mix (SYBR Green I) with a Bio-Rad CFX Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: The pure cultures were grown in TSAYE plates for 24 h and DNA was extracted using 2% Chelex solution (Chelex 100 resin, Bio-Rad laboratory, Hercules, California, USA). The colonies (2-3 mm ...
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bioRxiv - Microbiology 2020Quote: ... in oral swabs of positive and negative subjects for SARS-CoV-2 using magnetic bead-based multiplex immunoassays (Bio-Plex Pro™ human cytokine 27-plex panel, Bio-Rad Laboratories, Milan, Italy) according to the pre-optimized protocol 15 ...
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bioRxiv - Genetics 2020Quote: ... and ligated to MseI and EcoRI adaptors (Supplementary Table 4) at 37° C for 2 h in a T100™ Thermal cycler (Bio-Rad Laboratories, Hercules, CA). Success of the digestion/ligation reaction was confirmed on 1.5% of agarose gel electrophoresis ...
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bioRxiv - Genomics 2021Quote: ... Quantitative PCR was performed in triplicate and the 2−ΔCt method was used to calculate the expression of RUNX3 relative to β-actin (ID Assay qHsaCED0036269, Bio-Rad Laboratories, Kidlington, UK).
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bioRxiv - Immunology 2023Quote: ... was performed using 2×RealStar Green Fast Mixture (GenStar, Beijing, China) in a CFX384 Real-Time System C1000 Touch Thermal Cycler (Bio-Rad Laboratories, Hercules, CA, USA). The relative transcription levels of the genes of interest were normalized against the expression of GAPDH and calculated using the ΔΔCT method ...
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bioRxiv - Bioengineering 2022Quote: ... and EV/HSP27 mixtures containing 2 µg of HSP27 at the indicated w/w ratios were mixed with native sample buffer (cat#1610738, Bio-Rad Laboratories Inc., Hercules, CA) and loaded into the gel lanes ...
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bioRxiv - Biochemistry 2023Quote: ... The eluate was incubated for 60 min at room temperature with 50 mg of prewashed SM-2 Bio-Beads (Bio-Rad Laboratories, Hercules, CA, USA) under overhead rotation to ensure detergent removal.
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd, Hercules, CA, USA) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd) ...
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bioRxiv - Molecular Biology 2024Quote: ... were resolved on a 4-15% or 10% acrylamide gel and transferred onto a PVDF membrane (Invitrogen iBlot 2 or Bio-Rad Trans-Blot Turbo system). Membranes were blocked with 5% milk (standard protein detection ...
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bioRxiv - Cell Biology 2020Quote: ... 0.01% (w/v) bromophenol blue) and loaded on a 5% poly-acrylamide (29:1, BioRad) 0.5 x TBE gel ...
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bioRxiv - Cell Biology 2020Quote: ... blocked for 1 hour in 5% non-fat milk in TBS-T (Bio-Rad, USA), and incubated with primary antibodies anti-ACE2 (1:1000 ...
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bioRxiv - Synthetic Biology 2020Quote: ... We used 5% (w/v) skimmed milk in 1× tris-buffered saline (1706435, Bio-Rad) as the blocking reagent ...
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bioRxiv - Neuroscience 2024Quote: ... serum samples were diluted 1:5 with Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min ...
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bioRxiv - Biophysics 2023Quote: ... Membranes were then incubated with ECL solution diluted 1:5 in deionized water (BioRad #1705061) for 30 seconds ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were run in a 2% agarose slab gel and stained with ethidium bromide for visualization by UV shadowing (Bio-Rad Molecular Imager Gel Doc XR+).
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bioRxiv - Cell Biology 2020Quote: ... X-ray film (Figs 1-5) and the ChemiDoc MP Imaging System (Bio-Rad, cat#17001402) (Supplemental Fig 3).
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bioRxiv - Microbiology 2021Quote: ... Protein was applied at 1 ml/min to a 5 ml ceramic hydroxyapatite column (BioRad BioscaleTM) in the dialysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... and applied to a 5 mL prepacked Mini ceramic hydroxyapatite (CHT) type 1 column (Bio-Rad). The flowthrough was collected and purified by SEC using a HiLoad 16/600 Superdex 200 (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated with Tidyblot (diluted 1:5000 in 5% BSA in TBST, Biorad, USA), for 1 h ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with C-kit variant 1 forward primer 5’-CAACAAAGAGCAAATCCATCCC-3’ and reverse primer 5’-CATCACAATAATGCACATCATGCC-3’ with iQ SYBR Green supermix (BioRad, Hercules, CA, Cat No. 1708882). The PCR program was as follows ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were then blocked for 1 h at 25 °C with 5% non-fat dry milk (BioRad) in TBST (50 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 h at room temperature in 5% skim milk (Catalog #170-6404, Bio-Rad). Primary antibody incubations were performed overnight at 4°C with antibodies diluted in TBS/0.1% Tween-20/5% BSA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Synthesized cDNA was diluted 1:10 and 5 µl mixed with SsoAdvanced Universal Sybr Green supermix (Biorad). qPCR was performed using the Biorad CFX96 PCR machine ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated shaking for 1 hour at RT with 4% blocking solution (5% milk powder (BioRad) in TBS-T) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted 1:1000 in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...
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bioRxiv - Cell Biology 2020Quote: ... TBST (50 mM Tris-HCl pH 7.4, 1% Triton X-100) with 5% non-fat milk (Bio-Rad) was used for blocking ...
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bioRxiv - Genetics 2021Quote: ... Samples were then diluted 1:5 and placed into a 96 well plate with Supermix (no dUTPs, BioRad) and the required target locus primers and MGB-NFQ probes (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized with 1-5 μg of RNA using iScript cDNA synthesis kit (Bio-Rad). mRNA levels were measured using qRT-PCR with SYBR Green Master Mix (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... Immunohistochemistry was performed on paraffin sections (5 μm) using antibody F4-80 (Bio-rad, France; diluted 1:100). Sections were incubated overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked 1 hour in skim milk 5% TBS-Tween buffer (TBST, Bio-Rad, 0.05% Tween 20), and incubated overnight with the primary antibodies diluted in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 h at room temperature with 5% non-fat dry milk (Bio-Rad Laboratories) in Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked at room temperature for 1 hour in 5% nonfat dry milk (blocking grade, Bio-Rad) dissolved in Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Genetics 2021Quote: ... rotated at 4°C for 2h and then poured into a Econo-Column® Chromatography column (Bio-Rad, Hercules, CA). After extensive washing first with 80 mL of 20 mM Tris HCl [pH8@4°C] ...
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bioRxiv - Biophysics 2021Quote: ... and eletro-transfer at 100V for 2h were performed to separate protein extracts and transfer them to PVDF membranes (Bio-Rad). Non-specific sites were blocked with 5% BSA in TBS 0.05% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then transferred to a 0.2 µm nitrocellulose membrane (Amersham) in Tris/glycine/ 20% MeOH at 400 mA for 2h using a tank blot system (Bio-Rad). In order to detect any blotting artifacts the proteins on the membranes were visualized with Ponceau red solution ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker as follows: goat anti-mouse HRP conjugate (BioRad, 1706516; 1:5000), donkey anti-rabbit HRP conjugate (Amersham ...
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bioRxiv - Bioengineering 2020Quote: The cDNA was diluted 1/5 with dH2O and 2.5 µL were used for qPCR analysis (10 µL total volume; BioRad, iTaq Universal SYBR Green Supermix ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed 3 times with PBS and then blocked for 1 hour at room temperature with DPBS with 0.4% Triton X-100 and 5% non-fat dry milk (BioRad). Cells were stained with primary and secondary antibodies (Table S3 ...
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bioRxiv - Genomics 2021Quote: ... 1 μL diluted cDNA in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each sample we conducted three technical replicates and used their mean as the data point ...
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bioRxiv - Genetics 2023Quote: ... The membrane was blocked 1 hr with 5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad, Cat# 1706404XTU) and incubated overnight with the primary antibodies [TTC3 Polyclonal Antibody (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: 0.2 – 1 mL (sufficient to cover the sample) of 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA, USA) with 0.2% Tween20 and 0.1 mg/mL proteinase K (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 minute and then imaging every 10 seconds for 5 minutes on the ChemiDoc XRS+ machine (Bio-Rad). ImageLab (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was diluted 1:5 and RT-qPCR was done using SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad) with PrimePCR Assays (CCDC40 ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were blocked for 1 hour with 5% (w/v) skimmed milk powder in TBS-Tween 20 (Bio-Rad) (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... pooled and dialysed fractions were loaded onto a 1 mL ENrich™ Q 5 x 50 Column (Bio-Rad) for ion-exchange purification on the NGC system (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Xrn-1 digest samples were diluted 1:1 in 2X RNA loading dye (BioRad) and RNA was denatured at 80°C for 90 seconds ...
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bioRxiv - Biophysics 2020Quote: The prepared DNA samples (5 μL) were mixed thoroughly with 1 μL of 6X DNA loading buffer (Bio-Rad Laboratories). The mixtures were loaded into the wells of the prepared gel ...
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bioRxiv - Microbiology 2021Quote: ... and switch time linearly ramped from 1 s to 5 s over 14-15 h (CHEF DR III, Bio-Rad). DNA bands were visualized by staining the gel with a solution of 0.5 µg/mL ethidium bromide ...
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bioRxiv - Neuroscience 2021Quote: ... A 1:5 dilution from serum samples was combined with 4 °C Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories ...