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Citations for Bio-Rad :
951 - 1000 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... qPCR was performed with TaqMan Fast Advance Mastermix and primer pairs listed in Supplementary Table 2 and analysed using a CFX96TM Real-Time PCR System (Bio-RAD). Gene expression was normalised to glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA (2 μg) was subjected to cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). Real-time PCR was performed on an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... transferred to a nitrocellulose membrane (Cytiva) for 2 h (at 30V) and detected using infrared light on a Chemidoc MP (Bio-Rad).
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bioRxiv - Genetics 2024Quote: ... 2 µL of the diluted cDNA samples are supplemented with 10 µL of Supermix 2X ddPCR (without dUTP, Bio-Rad, #1863024), 1 μl of target probe (ZEN™ FAM)/primers mix (final concentration of 750 nM of each primer and 250 nM of probe ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA was amplified using the primers listed in Extended Table 2 and the SsoAdvanced universal SYBR Green supermix (Bio-Rad). Amplimers for vacA ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated for 1 h with 5% blotting grade blocker (Bio-Rad). Primary antibodies were incubated overnight at 4℃ and secondary HRP-conjugated antibodies were incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... After 1 h blocking (5% non-fat milk, Bio-Rad 170–6404) at room temperature (RT) ...
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bioRxiv - Microbiology 2024Quote: ... Diluted reaction products (1:5) were run on a 2.5% agarose (BioRad) gel in 1x TAE buffer (ThermoScientific ...
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bioRxiv - Immunology 2024Quote: ... Reaction was performed for 2h at 37°C in nuclease-free microcentrifuge tubes (BioRad). A260/295 was measured with a nanodrop (company ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified mouse α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified goat α-rabbit or goat α-mouse IgG conjugated to alkaline phosphatase ...
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bioRxiv - Genetics 2020Quote: ... nduo-5 and ctb-1 with act-1 as control by iQ™ SYBR® Green Supermix (BIO-RAD, #1708880).
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated in a 5 ml 1:1 A:B solution of Clarity Western ECL substrate (BioRad cat. #170-5060) for 5 minutes at room temperature with rotation ...
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bioRxiv - Molecular Biology 2021Quote: Amplification was carried out using PCR mixture-2 blue (AmpliSens, Russia) containing Taq-polymerase on a T100 Thermal Cycler (Bio-Rad, USA). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™ ...
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bioRxiv - Genomics 2022Quote: ... Each sample was tested by PCR systems (threshold – 0,050) to the presence of SARS-CoV-2 using a CFX Connect Real-Time PCR System (BioRad, Hercules, California, USA). RT-PCR were implemented in 40 μl of final volumes ...
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bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Microbiology 2021Quote: ... The amplified genomic DNA from the PPA strains was resolved using 2% agarose gel and visualized in the Gel DocTM XR+ documentation system (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... aeruginosa isolates were separated in 2% agarose gel and visualized their banding patterns in the Gel DocTM XR+ documentation system (Bio-Rad, USA).
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bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Master mixes containing primer pairs against FIBCD1 or B2M (Beta-2-microglobulin, housekeeping gene) were prepared with iTaq Universal SYBR Green Supermix (Bio-Rad #1725122). The mastermixes were dispensed into each well containing cDNA and incubated on ice for 15 minutes to allow the cDNA to dissolve ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were performed with 2 × Taq Master Mix (Vazyme, Nanjing, China) on a Bio-Rad Thermal Cycler (Bio-Rad, Hercules, CA, USA) using the following procedure ...
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bioRxiv - Genomics 2021Quote: ... PCR products were resolved using a 2% agarose tris-acetate-EDTA gel and a 177 bp band was visualized using the ChemiDoc™ imaging system (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
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bioRxiv - Neuroscience 2020Quote: ... and followed by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat antimouse IgG (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... which subsequently performed 2-DE using a 7 cm immobilized pH gradient (IPG) strips (pH range of 3—10, Ready strip, Bio-Rad, USA).
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bioRxiv - Cell Biology 2021Quote: ... Selected siRNAs were spotted onto a gridded MatTek dish (P35G-2-14-C-GRID) using a contact spotter (ChipWriter Pro-Bio-Rad Laboratories) resulting in a layout of 4 × 8 spots 40 ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd) ...
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bioRxiv - Neuroscience 2020Quote: Brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Developmental Biology 2021Quote: ... following by 25 cycles of preamplification using mix of 47 pairs for MTE primers, each at final 50nM concentration (Supplementary information, Supplementary Table 2) and SsoAdvanced™ PreAmp Supermix (Cat# 1725160, Bio-Rad). After amplification ...
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bioRxiv - Biochemistry 2021Quote: ... The purified proteins were running in 2-D gel electrophoresis and images were taken and analyzed in gel dock(Bio-Rad, USA). The details of protein purification protocols like TCA/Acetone ...
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bioRxiv - Plant Biology 2021Quote: ... The pelleted nuclei were re-suspended in 200 µl of NIB and mixed with a 140 µl aliquot of melted 2% LMA agarose (Bio-Rad, 1703594) at 43°C ...
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bioRxiv - Microbiology 2020Quote: ... Non-human primate samples were inactivated with γ-radiation (2 MRad) according to standard operating procedures and assayed on a Bio-Plex 200 instrument (Bio-Rad) using the Non-Human Primate Cytokine MILLIPLEX map 23-plex kit (Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were resuspended in MACS buffer (PBS +2% FBS +2mM EDTA) and counted using trypan blue with the TC20™ Automated Cell Counter (Bio-Rad). In some cases ...
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bioRxiv - Plant Biology 2020Quote: ... Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 μl, BioRad). Before loading ...
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bioRxiv - Microbiology 2020Quote: Serum samples were inactivated with γ-irradiation (2 mRad) and cytokine concentrations were determined on a Bio-Plex 200 instrument (Bio-Rad) using Milliplex Mouse Cytokine/Chemokine MAGNETIC BEAD Premixed 25 Plex Kit (Millipore) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 10 μL of reconstituted representative influenza vaccine (i.e., AddaVax/Y-2) of Fluad Quadrivalent was mixed with Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and β-mercaptoethanol (2% ...
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bioRxiv - Neuroscience 2020Quote: ... brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad, Veenendaal, The Netherlands).
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bioRxiv - Cell Biology 2021Quote: ... 1∼2 μg of total protein for each lane) was loaded on a precast 4-20% gradient polyacrylamide gel (PAGE, Bio-Rad: 4561096) and electrophoresed at a constant voltage of 200 V for 35 min ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were detected by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat anti-mouse IgG (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Immunology 2023Quote: The detection of IgG ANAs in the serum of Ngfr KO and Bcl2 Tg mice was performed by IF in Hep-2 coated slides (Kallestad™ Bio-Rad) following manufacturer instructions ...
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bioRxiv - Genetics 2023Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd.) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd.) ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using gDNA of purified viruses was performed in parallel of each experiment ...
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bioRxiv - Biophysics 2022Quote: ... Samples were centrifuged for 2 min at 12,000 x g before being separated on 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) at 160 V for 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Biophysics 2024Quote: ... and incubated for 3 hours at 37 °C and 5% CO2 with GPMV-vesiculation buffer consisting of 25 mM methanol-free formaldehyde (FisherScientific, cat# 11586711) and 2 mM dithiothreitol (Biorad, cat# 1610610) dissolved in HBSS ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted four-fold and 2 µL was used for quantitative PCR (qPCR) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725274) and 500 nM of gene-specific qPCR primers (see Table S2 ...