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Citations for Bio-Rad :
1051 - 1100 of 3002 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
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bioRxiv - Microbiology 2022Quote: ... Reaction was developed using a freshly prepared TMB substrate solution and stopped with 1M Sulfuric acid before determining the OD450 using a microtiter plate reader (iMark mic, Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were then stained with Coomassie Blue solution, followed by de-staining (Methanol, Acetic Acid solution) and visualised by using Gel Doc™ XR+ system (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... and then the gel was washed in destain buffer (8% acetic acid) until the background was washed off for visualization by ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: Intracellular and soluble-secreted proteins were Deoxycholate/Trichloroacetic acid– precipitated and separated by SDS-PAGE on gradient 4-20% PROTEAN TGX Stain-Free Gels (Bio-rad) and proteins were transferred to polyvinylidene difluoride membranes within Trans-Blot Turbo Transfer Packs (Bio-rad ...
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bioRxiv - Cell Biology 2023Quote: ... for protein extracted from cultured cells or brain sections and micro bicinchoninic acid (microBCA) for protein extracted from EVs according to the manufacturer’s instructions (Bio-Rad Laboratories).
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bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... After gel polymerization was performed pre-electrophoresis of empty gel in 5% acetic acid (running buffer) for 1-1.5h/150 V in reverse polarity of electrophoretic BioRad system (Bio-rad, USA). The running buffer was discarded ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Protein samples were heated at 55°C for 10 min and 10 μl was loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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bioRxiv - Microbiology 2020Quote: ... concentration of total aerobic GNB and of MDR-GNB were determined by plating serial dilutions (pure, 10−2, 10−4) of initial faeces or EA sample onto Drigalski agar (Bio-Rad) with or without 1mg/L cefotaxime ...
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bioRxiv - Microbiology 2021Quote: Two micrograms of each RNA sample was loaded on 10% polyacrylamide gels containing 7 M urea or loaded onto 10% Criterion TBE-urea precast gels (Bio-Rad) and electrophoresed at 85 V ...
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bioRxiv - Genetics 2021Quote: ... Samples were denatured at 100°C for 10 min and 5% of samples were loaded and run on a 10% Mini-Protean TGX gel (Bio-Rad). Electrophoresed proteins were transferred onto a nitrocellulose membrane using Turbo-blot Transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated 10 min at 95°C before loading 10 µl aliquots onto precast mini- PROTEAN TGX gels (Bio-Rad). The polyacrylamide concentration was 7.5% for GFP-DamX constructs ...
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bioRxiv - Cancer Biology 2023Quote: ... Totals of 10-30 µg of protein lysate and 10 µL of dual color protein standard ladder (Bio-Rad #161-0374) were loaded onto 4-20% Mini-PROTEAN gels (Bio-Rad #4568094 ...
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bioRxiv - Biochemistry 2022Quote: ... Dialysis was repeated against another 1 L of low-salt buffer for 3 h before application to a MonoQ (MonoQ HR 10/10, Pharmacia) FPLC column (Biologic Duoflow system, Bio-Rad). The sample was eluted over 80 mL in 20 mM piperazine by applying a gradient from 0% to 30% of a high-salt buffer (20 mM piperazine ...
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bioRxiv - Neuroscience 2023Quote: ... The prepared NDs were purified by SEC using a Superdex 200 Increase 10/300 GL column (Cytiva) connected to an NGC Quest 10 Plus system (Bio-rad) and the standard buffer as the running buffer at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Protein (10 ug) from each sample was treated with ß-mercaptoethanol and subsequently electrophoresed on precast 4%–10% gradient gels (Biorad), as previously described (Alcantara et al. ...
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bioRxiv - Microbiology 2023Quote: ... and was further cleared by size exclusion chromatography (Superdex 200 10/300 GL column (Cytiva) attached to an NGC chromatography system Quest 10 plus (Bio-Rad)) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 95 ℃ for 10 minutes and resolved by SDS-PAGE using 4-20 % or 10 % precast gels (BioRad, 4561093), depending on the molecular weight of the desired protein ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins (10–30 μg) were run on a 10% SDS–polyacrylamide gels and then transferred to a nitrocellulose membrane (Biorad®). The membranes were blocked in 5% lowfat milk-PBS-tween solution and then incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitated DNA and 10% of corresponding input DNA (1:10 diluted) were used for qPCR with SsoAdvanced Universal SYBR Green supermix (Bio-Rad) using CFX 96 (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... These samples were spun down for 5 min at 16000× g before loading 10 µL into 10 % Mini-PROTEAN TGX precast gels (Bio-Rad). The gel was run on gel electrophoresis at 150 V for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were heat at 95°C for 10 min and then separated by SDS-PAGE electrophoresis on 10% acrylamide gels (FastCast™ Acrylamide kit, BioRad) at 110V for 60 min ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured for 10 min at 95°C and then 10 µl were loaded onto a precast 4-15% protein gel (Bio-Rad). Electrophoresis was performed by applying a constant voltage of 200 V in SDS running buffer (Tris/Glycine/SDS ...
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bioRxiv - Neuroscience 2021Quote: ... in the HPT and HPC were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using TGX Stain-Free 12% gels (gels were visualized using ChemiDoc Imaging Systems, Bio-rad, Hercules, California, USA) and transferred to nitrocellulose membranes using Trans-Blot Turbo Transfer System (Bio-rad ...
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bioRxiv - Biochemistry 2021Quote: The purified SdGA was analyzed by SDS-PAGE using 12% (w/v) gels as described by Laemmli [35] with a Bio-Rad Mini Protean system (Bio-Rad, Hercules CA, USA). Gels were stained by Coomassie Blue and/or electro-blotted onto nitrocellulose membranes (HybondTM-ECLTM ...
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bioRxiv - Physiology 2020Quote: ... A sample (50 µg) was fractionated by SDS/PAGE on 12% (w/v) polyacrylamide gels (TGX StainFree FastCast gel, Bio-Rad Inc., CA, USA), exposed to UV for 1 min and total protein patterns were visualized using ChemiDoc MP Imager (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... resolved on the 12% SDS PAGE and transferred to nitrocellulose membrane (Trans-Blot Turbo Midi Nitrocellulose Transfer Pack, Bio-Rad, 0.2 μm pore size) using Trans-Blot Turbo Transfer Starter System (Bio-Rad ...
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bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
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bioRxiv - Microbiology 2021Quote: ... For western blot analysis samples were run on 12% SDS-PAGE under reducing conditions and transferred to PVDF membrane (Bio-Rad, Cat. No. 1620177). SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Biophysics 2020Quote: ... Pure ClyA-AS type-I (12-mer) nanopores were obtained using native PAGE on a 4-15% gradient gel (Bio-Rad, Veenendaal, The Netherlands) and subsequent excision of the correct oligomer band.
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts (50 μg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 µg of each protein mixture was separated by SDS-PAGE (polyacrylamide gel electrophoresis) on a Criterion XT 4-12% Bis-Tris gel (Biorad Laboratories cat. No. 3450124). Proteins were transferred to a PVDF membrane (Merck Millipore cat ...
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bioRxiv - Cell Biology 2021Quote: ... Bead pellets were resuspended and boiled in β-mercaptoethanol containing Laemmli sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts (50 µg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cell Biology 2022Quote: ... The radio-labelled proteins were then separated with SDS-PAGE on a 4-12% Criterion XT Bis-Tris gels (Bio-Rad, Hercules, CA, USA) in MES buffer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... A NF54 WT culture with at least 5% ring stages was transfected with 100 µg plasmid DNA in transfection buffer via electroporation (310 V, 950 μF, 12 ms; Bio-Rad gene-pulser Xcell) as described (e.g ...
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bioRxiv - Neuroscience 2023Quote: 15-20 µg of protein were loaded onto 4-12% Bis-Tris gels (Novex, Cat. no: NP0336BOX) or 4-20% Tris-Glycine extended gels (BIO-RAD, Cat. no: 4561095) for separation and transferred to nitrocellulose or methanol activated PVDF membranes ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded onto 4-12% Criterion XT precast gels and proteins were separated by SDS-PAGE under reducing conditions (Bio-Rad Laboratories, Hercules, CA). The proteins were then electroblotted onto a 0.45 μm nitrocellulose membrane ...
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bioRxiv - Microbiology 2024Quote: ... purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a 12% polyacrylamide gel using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated on an 8-12% SDS-polyacrylamide gel and blotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), The membrane was incubated overnight at 4°C with primary Abs ...
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bioRxiv - Microbiology 2024Quote: ... Elution fractions of protein purifications were analyzed on 12 % SDS-PAGE gels and the protein concentrations were determined by Bradford (Bio-Rad Laboratories, Hercules, California) or Qubit protein assay (Thermo Fisher Sceintific ...
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bioRxiv - Microbiology 2023Quote: ... Total cell extracts or fractions from protein purification were resolved on 12% SDS-PAGE and transferred to a nitrocellulose membrane using a TransBlot® semi-dry transfer unit (Bio-Rad Laboratories, Inc) and transfer buffer (48 mM Tris base ...
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bioRxiv - Microbiology 2023Quote: ... A WT NF54 culture with mainly ring stages was used to transfect with 100 µg plasmid DNA in transfection buffer via electroporation (310 V, 950 μF, 12 ms; Bio-Rad gene-pulser Xcell) as described earlier (e.g ...
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bioRxiv - Cell Biology 2023Quote: ... samples were boiled at 97°C for 5 min and run on a 12% Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hercules, USA) for 1 hour at 150V ...
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bioRxiv - Plant Biology 2023Quote: ... Seed storage proteins were visualized from 100 µg of seed material on a 12% SDS-PAGE compared to a Precision Plus Dual Color Protein Standard (Bio-Rad, Hercules, CA, USA). Gels were stained for 1 hr using a 0.1% Coomassie Brilliant Blue ...
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bioRxiv - Systems Biology 2023Quote: ... resolved in a 4-12% Invitrogen B-T gel using MOPS buffer and transferred onto a polyvinylidene difluoride (PVDF) membrane using BioRad transblot (Bio-Rad, Hercules, CA, USA). The membrane was blocked with 5% skim milk ...
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bioRxiv - Neuroscience 2023Quote: ... Forty µg denatured protein per well was resolved by 8-12% SDS- PAGE homemade gels in parallel with multi-colored molecular weight marker (Bio-Rad Laboratories, Cat.No.1610395). Gels were run at 100V for 1-2 h and transferred overnight at 8- 12V ...
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bioRxiv - Immunology 2023Quote: ... PE-conjugated anti-Foxp3 mAb (clone FJK-16s, eBioscience™, 1:20) and/or Alexa Fluor 647-conjugated anti CD3 mAb (clone CD3-12, Bio-Rad, 1:100) were added directly to cells and incubated for 40 min ...