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Citations for Bio-Rad :
901 - 950 of 2823 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was then destained for 15 minutes in 7.5% (v/v) acetic acid before being imaged on a Chemidoc imaging system (Bio-Rad). For western blots ...
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was measured using a bicinchoninic acid (BCA) protein assay (Pierce) or Bradford assay (Bio-Rad, Cat. No. 5000006). Proteins were prepared in 1× Laemmli buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed on a 4-15% polyacrylamide NuPage Bis-Tris gel (invitrogen) in 1x morpholineethanesulfonic acid (MES) SDS running buffer (invitrogen) or on a 4-20% polyacrylamide Criterion gels (BioRad) in 1x Tris/glycine/SDS buffer (BioRad ...
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bioRxiv - Cell Biology 2020Quote: 10-40 µg of total protein lysate were separated by SDS-PAGE on 8% or 10% polyacrylamide minigels (BioRad) and transferred onto a nitrocellulose membrane (GE healthcare ...
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bioRxiv - Microbiology 2019Quote: ... and boiled for 5-10 min before being run on 10-12.5% SDS-PAGE or precast Mini-Protean TGX (Biorad) gels ...
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bioRxiv - Cell Biology 2020Quote: ... and loaded onto 10% SDS-PAGE gel (TGX™ Fast Cast™ Acrylamide Kit 10%, BioRad, California, United States). Proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ semi-liquid rapid transfer system (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... fixed in 10% (v/v) Neutral-buffered Formalin for 1.5 hrs and blocked in 10% donkey serum (Bio-Rad) for 2 hrs ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted proteins were separated by NuPAGE 4-12% Bis-Tris gel in MES SDS running buffer and transferred to a PVDF membrane (Bio-Rad, 162-0263). Tubulin beta chain protein was detected with an anti-TUBB antibody (1:500 in 5% fat-free milk solution in TBST ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg samples were boiled in gel loading buffer containing β-mercaptoethanol as a reducing agent and loaded onto a 12% or “AnyKD” precast polyacrylamide gel (Bio-Rad 4569033) for western blot analysis ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled at 100°C for 5 min prior to SDS-PAGE separation on 12% acrylamide Mini-protean TGX precast gels (Bio-Rad Laboratories, US) and Western blot analysis.
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bioRxiv - Neuroscience 2022Quote: ... The nascent and total protein samples were then run on a 4-12% gradient SDS-polyacrylamide gel along with Precision Plus Protein Kaleidoscope ladder (Bio-Rad, Cat #1610395). Then the proteins were transferred onto nitrocellulose membranes using either a Bio-Rad Trans-Blot Turbo Transfer system (25V ...
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bioRxiv - Bioengineering 2022Quote: ... and an equivalent amount of protein lysate (12-15 µg) was electrophoresed on a 4-12% NuPAGE gradient gel and transferred onto low florescent polyvinylidene difluoride membranes (Bio-Rad, Cat: 1704274). Immunoblotting was performed with primary antibodies followed by secondary antibodies with the indicated dilutions ...
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bioRxiv - Microbiology 2022Quote: The protein samples denatured in 1x SDS sample buffer were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) in a 12% gel before blotted to polyvinylidene difluoride (PVDF) membrane by the wet transblot system (Bio-Rad, California, USA). The blocked membrane was incubated with the primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein (5–20 μg) was resolved onto 8–12% SDS-PAGE and blotted onto 0.2 μm pore size PVDF membranes (1620177, Bio-Rad, Hercules, CA, USA). Primary antibodies were incubated overnight at 4°C after blocking membranes for 1h ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were boiled (95°C) and 18 µL were loaded onto a 4-20% Mini-PROTEAN®TGX™ 12 well-gel (Biorad®). After transfer onto a PVDF (Amersham Hybond ...
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bioRxiv - Cell Biology 2020Quote: ... Equal proportions of supernatants and pellets were run on 4-20 % gradient or 12 % SDS-polyacrylamide gels (Mini-PROTEAN TGX Precast Gels, Bio-Rad Laboratories Inc.), which were then stained with Coomassie Blue ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled and loaded onto a 12% precast Tris-Tricine gel in a mini-Protean apparatus (both from Bio-Rad, Hercules, CA). Gels were stained using SimplyBlue SafeStain (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: ... samples were analyzed by SDS-PAGE (either 4-20% Tris-glycine or 4-12% Bis-tris pre-cast gel, Bio-rad Laboratories, Inc.) and transferred to nitrocellulose membranes (Bio-rad Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... Protein separation was carried out via SDS-PAGE in 12% polyacrylamide gels (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad, Munich, Germany) in TGS buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... 1976) samples mixed with loading dye were loaded on the polyacrylamide matrix (Mini-PROTEAN® Tetra System 12-0625 BioRad, Hercules, California, USA). To detect the separated ADH isoforms ...
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bioRxiv - Cancer Biology 2021Quote: Cellular lysates were incubated at 95°C for 5 min and separated in 12% Mini-PROTEAN TGX Precast Protein gels (BIO-RAD, Cat #4561044). Proteins were then transferred to 0.45 μm PVDF membrane at 100V for 1 hr ...
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bioRxiv - Biophysics 2021Quote: ... 100 μg of total-proteins was then subjected to electrophoresis in 4-12 % Bis-Tris Plus Gels (Novex®, NW00125BOX) and transferred onto PVDF membrane using a Trans-Blot® Turbo™ Transfer System (BIORAD). After blocking with 5 % non-fat milk ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by 10 % and 12% SDS-PAGE gels and transferred to a PVDF membrane followed by blotting with Clarity Western ECL substrate (Bio-Rad, cat#1705061).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lysates were loaded onto 4-12% Criterion XT precast gels and resolved by SDS-PAGE under reducing conditions (Bio-Rad Laboratories, Hercules, CA). The proteins were then electro-blotted onto a 0.45 µm nitrocellulose membrane ...
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bioRxiv - Genetics 2022Quote: ... were electrophoresed on a 12% polyacrylamide gel and then transferred on PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, CA). Membranes were incubated with primary and secondary antibodies ...
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bioRxiv - Synthetic Biology 2022Quote: ... 0.02% Bromophenol blue) without a subsequent boiling step and loaded (13ul) using commercially available precast 12% SDS-PAGE gels (BioRad, Singapore; Cat: 4561046). Gels were illuminated with a blue light transilluminator ...
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bioRxiv - Microbiology 2023Quote: ... 12 μl of purified recombinant orthologous proteins at a concentration of 250 nM were loaded onto a TGX FastCast 12% stain-free gel (Bio-Rad, Feldkirchen, Germany). Electrophoresis was conducted at 220 V for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... and separated in polyacrylamide gels at 100-140 V on ice (4-15%, Mini-PROTEAN TGX, Bio-Rad; or as described previously [12]). To detect protein-bound acrolein in spinal cords ...
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bioRxiv - Immunology 2023Quote: ... Same protein amounts were loaded onto 4-12% NuPAGE Gels and protein was transferred to PVDF membranes by using Trans-Blot® Turbo™ Transfer System (Biorad#17001917). Membrane was blocked with 5% milk-TBS ...
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bioRxiv - Immunology 2023Quote: ... Equal volumes of lysates were run on 12% SDS-polyacrylamide gels and transferred at 25 V onto PVDF membranes using a Trans-Blot® Turbo Transfer™ System (Bio-Rad). Membranes were blocked in 5% BSA (w/v ...
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bioRxiv - Genetics 2023Quote: ... Bromophenol blue buffer was loaded on 1.0 mm thick Criterion XT Tris acetate (poly-acrylamide) gels with a linear resolving gel gradient of 4-12% (Biorad; Lunteren, The Netherlands). Gels were run at 75 V (0.07 A ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were analyzed by SDS-PAGE using 12% gels and visualized on a ChemiDoc XRS Imaging System (Bio-Rad Laboratories GmbH, München, Germany) using the Image Lab™ software and pre-programmed option for Coomassie stained gels or Alexa488/Alexa647 fluorophores ...
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bioRxiv - Molecular Biology 2024Quote: ... Twenty micrograms of whole cell extracts were resolved on 4−12% SDS-polyacrylamide gels and transferred onto Ready gel blotting sandwiches immune-blot PVDF membranes (Biorad, Hercules, CA, USA) using a XCell II™ Blot Module (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... boiled (95°C, 10 min), centrifuged (1,000 × g, 2 min) and loaded on 10% or 4-20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Cell Biology 2020Quote: ... Equal protein content for each condition (targeting 10 μg) was run on 10% Mini-PROTEAN TGX precast protein gels (Biorad). After electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... 95°C), and run (loaded in 2 wells, 20 μL per well) on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mg/ml Orange G), denatured (2 min, 80°C) and run on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then transferred onto low-fluorescence 0.45 μm PVDF membranes with transfer buffer (10× Tris/glycine with 10% methanol) (Bio-Rad) for 10 min using the semi-dry Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... Equal protein content for each condition (targeting 10 μg) was run on 10% Mini-PROTEAN TGX precast protein gels (Biorad). After electrophoresis ...
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bioRxiv - Biophysics 2021Quote: ... were pooled and buffer exchanged to remove excess imidazole (20mM sodium phosphate, pH 7.5, 100mM NaCl, 10% glycerol) using a 10-DG column (Bio-Rad; pre-equilibrated in elution buffer without imidazole and DTT) ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated from 10 °C to 95 °C (10 s at each 0.5 °C step) using a CFX Connect qPCR (Bio-Rad). Melting temperatures were calculated with DSFWorld61 using model 1 in the “by sigmoid fitting” option ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to PVDF membrane at 4°C for 14.5 h at 30 V using the mini trans-blot module in transblot buffer (10 mM MES buffer pH 6 and 10% (v/v) methanol) according to the supplier’s instructions (Bio-Rad). The membrane was removed from the cassette ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... 5 µg of protein was loaded per well on a 10% TGX 10-well 30µl/well gel (456-1033, Bio-Rad) for 1 hr ...
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bioRxiv - Pathology 2022Quote: ... 1 or 10 μg of total protein were loaded and separated by SDS–PAGE on 10% mini gels (Bio-Rad), and transferred into a 0.2 μm-pore size nitrocellulose membrane (#GE10600001 ...
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bioRxiv - Molecular Biology 2022Quote: ... 20-100 µg of sheared chromatin used as input and ChIP beads were then boiled 10 min in 100 µL H2O containing 10% (V/W) chelex resin (BioRad), followed by Proteinase K (0.2 mg/mL)-digestion for 30 min at 55°C ...
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bioRxiv - Microbiology 2023Quote: ... 10 min), centrifuged (1,000 x g, 2 min) and loaded on 10% or 4–20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Microbiology 2023Quote: ... and 0.2M DTT) for 10 minutes at 95°C before loading in a 10% SDS-PAGE Gel (Bio-Rad 4561034). Sample proteins were separated in 1X SDS Running Buffer (25mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... were separated on 10 % Tricine gels, and then transferred (0.5 A, 25 V 10 min) onto 22 µm PVDF membranes (Bio-Rad). The membranes were washed 3 times with 1x TBST and then blocked with 5 % bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... 10-50 μg protein was loaded on 10% acrylamide home-made or gradient (4-15% acrylamide) commercial (BIO-RAD, 4561086) Tris-Glycine-SDS gels along a protein ladder (PageRuler prestained protein ladder ...
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bioRxiv - Molecular Biology 2023Quote: ... and qPCR primers at 10 µM in a total volume of 10 µL in a ≥ 40-cycle and melt curve reaction cycler (Biorad, Base #CT009383 ...