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Citations for Bio-Rad :
1051 - 1100 of 4573 citations for 14 Nonylphenoxy 3 6 9 12 tetraoxatetradecan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... were performed using the FIREPol® Master Mix Ready to Load (Solis biodyne, 04-12-00115) and a T100 thermal cycler (Biorad). Sequences of the primers used are the following ones ...
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bioRxiv - Cell Biology 2022Quote: ... Twenty to 40 µl of the lysate were loaded into a 12 % polyacrylamide gel for SDS-PAGE electrophoretic (BioRad. Mini-PROTEAN) separation of proteins ...
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bioRxiv - Cell Biology 2022Quote: ... separated into an 8%-12% SDS-PAGE gels and transferred to a nitrocellulose Hybond-C membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% milk in PBS-0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Polyacrylamide gels (shear modulus: 16 kPa—final concentrations of 12% acrylamide [1610140, Bio-Rad] and 0.15% bis-acrylamide [1610142, Bio-Rad]) were embedded with 0.04-μm fluorescent microspheres (F8789 ...
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bioRxiv - Cell Biology 2022Quote: ... Polyacrylamide gels (shear modulus: 16 kPa—final concentrations of 12% acrylamide [1610140, Bio-Rad] and 0.15% bis-acrylamide [1610142, Bio-Rad]) were embedded with 0.04-μm fluorescent microspheres (F8789 ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with beta-mercaptoethanol were boiled for 5 min and separated on 12% SDS-PAGE gels prior to transfer to methanol-activated PVDF membranes using the Trans-Blot Turbo system (Bio-Rad). Membranes were blocked in Tris-buffered saline with Tween-20 (TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... were separated on 12% (w/v) SDS-PAGE gels and transferred to an Immun-Blot low fluorescence-PVDF membrane (Bio-rad). The membrane was first blocked with Odyssey Blocking Buffer (TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Bioengineering 2023Quote: ... Approximately 5 x 108 vesicles and purified HaloTag standard curve were loaded and run on a 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) at 150 V for 90 min or 50V for 10 min followed by 100V for approximately 70 min ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... pulldown samples collected as described above were loaded on a 4-12% gradient Criterion XT Bis-Tris precast gel (Bio-Rad). The gel was fixed in 40% methanol and 10% acetic acid and subsequently stained for 1h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Treated whole cell extracts (5 µg per lane) were resolved on a 12% TGX Stain-free polyacrylamide gel (Bio-Rad, #1610185) by applying 180V until loading dye ran off the gel ...
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bioRxiv - Physiology 2024Quote: ... The proteins were subsequently separated via electrophoresis using 12% SDS-PAGE gels and transferred onto a PVDF membrane (Trans-Blot Turbo, Bio-Rad). S-nitrosylated Cx43 proteins were detected using a mouse anti-connexin-43 antibody (#C8093 ...
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bioRxiv - Genomics 2024Quote: ... Proteins were extracted as described previously,53 and separated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad). Transfer to the membrane was performed with iBind Western System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Equal amounts of bone marrow cell lysates underwent electrophoresis on a gradient SDS-PAGE gel (Criterion XT precast gel, 4-12%, BioRad, 3450123). Proteins were transferred onto nitrocellulose membranes and after blocking with Intercept (TBS ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 15 μg protein was separated by SDS-PAGE on a precast 4-12% Criterion XT Bis-Tris gel (Bio-Rad) and transferred to a nitrocellulose membrane using TurboBlot (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were boiled for 5-10 minutes and then separated using SDS-PAGE with 4-12% Criterion XT Bis-Tris Gels (Bio-Rad). The proteins were transferred to PVDF membranes (Merck Millipore) ...
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bioRxiv - Microbiology 2024Quote: ... protein concentrations were measured using the BCA assay and 20 μg of proteins from whole cell lysates were resolved on pre-cast 12% tris-glycine PAGE (Bio-Rad) followed by transfer to a LF-PVDF membrane using a Bio-Rad semi dry transfer apparatus ...
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bioRxiv - Biochemistry 2024Quote: ... Equal amounts of samples were run on SDS PAGE gels (BIO-RAD 4-12% gradient Bis-Tris or XT Precast gels with catalog numbers mples were run on SDS PAGE gels (BIO-RAD 4-12% gradient Bis-Tris or XT Precast gels with catalog numbers #3450123 or #3450124 ...
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bioRxiv - Synthetic Biology 2024Quote: ... the samples were analyzed by western blotting using equal protein amounts of cell lysates separated by SDS–PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Multiplex 24 μL ddPCR reactions were prepared by mixing 12 μL of ddPCR supermix (no dUTP; Bio-Rad, catalog no. 1863024), forward and reverse primers for target and reference genes (IDT ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µL of the cleared lysates and 10 µL of washed beads after pull-down were applied on separate Mini-PROTEAN TGX 4-12% polyacrylamide gels (BIO-RAD), and run for 45 min at 200 V in Tris/Glycine/SDS buffer ...
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bioRxiv - Genetics 2024Quote: ... Samples were loaded at 15-20 µg per well onto a NuPAGE 4-12% Bis-Tris Acrylamide midi gel along with Precision Plus dual color ladder (BioRad, 1610374). The gel was run on an XCell4 Surelock Midi-Cell (ThermoFisher WR0100 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were analyzed on either a 12% Bis Tris PAGE gel (for histone analytes) or a 4-12% Bis Tris PAGE gel (for all other analytes) using 1x MES running buffer (Bio-Rad) at 180 V ...
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bioRxiv - Plant Biology 2021Quote: ... At least 4-6 technical replicate RT-qPCR reactions were performed using iTAQ with ROX and SYBR (BioRad), and 20μL reactions were prepared as per the manufacturer recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.032 % (w/v) SM was applied to a centrifugal device (Micro Bio-Spin 6, Bio-Rad, Hercules) to exchange the buffer to a MS buffer containing 200 mM ammonium acetate (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was purified by loading quenched samples onto Micro Bio-spin Columns with Bio-Gel P-6 (BioRad), followed by ethanol precipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by 6 washes in 100 µL of PBST in Bio-Spin columns (catalog number 7326204, Bio-Rad). The bound immunocomplexes were eluted via 0.1 M glycine ...
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bioRxiv - Biochemistry 2021Quote: KirBac3.1 protein was buffer-exchanged to 200 mM ammonium acetate (pH=8.0) with 0.5% C8E4 (2×CMC) using a Biospin-6 column (BioRad). The protein sample was loaded into a gold coated needle prepared in-house and analysed on a modified Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 6 x 5 minutes with TBS-T before developing with Clarity Western ECL Substrate (Bio-rad).
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bioRxiv - Microbiology 2023Quote: ... separated on 6% acrylamide 8 M urea gels and transferred to Zeta Probe GT membranes (Bio-Rad laboratories) by electroblotting ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then cleaned 2-times on Micro Bio-Spin™ P-6 gel Columns (BioRad, #732-6221) where buffer was exchanged with phase separation buffer ...
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bioRxiv - Biochemistry 2022Quote: ... solutions containing purified CytbX were buffer-exchanged into 0.2 M ammonium acetate (pH 8.0) containing 2x CMC of Lauryldimethylamine oxide (LDAO) using Biospin 6 columns (Biorad). 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... then 6 L of RO water with Ca2+ chelating beads (BIO-RAD Chelex 100 Resin Cat#142-1253), and finally 6 L of RO water alone ...
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bioRxiv - Molecular Biology 2024Quote: ... sampels were separated on 6– 15% SDS–PAGE and proteins were transferred to nitrocellulose membrane (1620115, Bio-Rad) using the Trans-Blot Turbo Transfer System from Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Homemade 4% stacking and 6% running gels were run at 60-80V in Tris/Glycine/SDS buffer (BioRad). Transfer was done overnight in 10% methanol in Tris/Glycine buffer (BioRad ...
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The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagybioRxiv - Cell Biology 2024Quote: ... and 150 mM NaCl utilizing a Bio-Scale Mini Bio-Gel P-6 desalting column (Bio-Rad Laboratories). Subsequently ...
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bioRxiv - Synthetic Biology 2024Quote: ... Images were acquired using a Chemidoc Imaging System and analyzed with Image-Lab v.6 software (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Precision Plus Protein Dual Color Standards Protein Marker was used as a marker (6 μl, #1610374, BIO-RAD).
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bioRxiv - Plant Biology 2020Quote: Forward and reverse new PCR primers for the 12 Zn transport-related genes and the two reference genes suitable for SYBR® Green II RT-qPCR assays (Biorad, USA) were designed (Table 2) ...
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bioRxiv - Molecular Biology 2020Quote: Protein lysates were separated on 12% SDS polyacrylamide gels and transferred to PVDF membranes using the Trans-Blot Turbo System (BioRad, Feldkirchen, Germany). Equal protein loading and successful blotting was verified by Coomassie Brilliant Blue staining ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein per lysate were loaded onto Criterion XT 4-12% Bis-Tris polyacrylamide gels (Bio-Rad, Hercules, CA), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total cell proteins were run in a 12% SDS-polyacrylamide gel and blotted to the PVDF membrane (Bio-Rad, USA). Membranes were incubated overnight at 4 °C with mature BDNF primary antibody (ThermoFisher OSB00017W ...
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bioRxiv - Molecular Biology 2021Quote: ... for the experiments done on 12-well plate) for each sample on SDS-PAGE using 4–20% Mini-PROTEAN® TGX gels (Bio-Rad), and immunoblotted as described (36) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and MgCl2 [12.5 mM]) and annealed by cooling the solution from 95 to 12 °C over 30 min in a thermal cycler (Bio-Rad, T100). Detailed descriptions for the assembly of individual gate complexes are described in the supplementary information ...
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were resolved by SDS-PAGE 12% self-cast gel and transferred onto polyvinylidene difluoride (PVDF) membrane using the Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) in transfer buffer (25 mM Tris ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... Proteins were separated on SDS-containing 8 to 12 % polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories, Inc., Hercules, USA). After staining with primary and secondary antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... The treated lysates were mixed with the 2 × SDS-PAGE sample buffer with or without 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...