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Citations for Bio-Rad :
1001 - 1050 of 1185 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 5-10μg of total proteins were separated on bis-tris polyacrylamide gradient gels (NuPAGE Novex 4-12% Bis-Tris Gel, Life tech, #NW04120BOX) or TGX stain-free gels (BioRad, #4568126) and transferred to PVDF membranes ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Bioengineering 2023Quote: ... Approximately 5 x 108 vesicles and purified HaloTag standard curve were loaded and run on a 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) at 150 V for 90 min or 50V for 10 min followed by 100V for approximately 70 min ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... pulldown samples collected as described above were loaded on a 4-12% gradient Criterion XT Bis-Tris precast gel (Bio-Rad). The gel was fixed in 40% methanol and 10% acetic acid and subsequently stained for 1h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Microbiology 2023Quote: Dilution series containing equal amounts of protein were prepared and separated on 12% Mini-PROTEAN TGX gels (BioRad, Hercules, CA, USA) by SDS-PAGE and then transferred onto 0.2 µM polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... An equal amount of sample was loaded and separated on a 12% bis-tris gel and transferred to PVDF membranes (1620177 Bio-Rad) using a Bio-Rad cassette at 70 volts for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured by 5 min incubation at 95°C before running them on a TGX 4–12% or 10% gel (Bio-Rad) and transferred onto nitrocellulose membrane for hybridization with specific antibodies.
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bioRxiv - Neuroscience 2024Quote: ... Approximately 15 μg protein was separated by SDS-PAGE on a precast 4-12% Criterion XT Bis-Tris gel (Bio-Rad) and transferred to a nitrocellulose membrane using TurboBlot (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were boiled for 5-10 minutes and then separated using SDS-PAGE with 4-12% Criterion XT Bis-Tris Gels (Bio-Rad). The proteins were transferred to PVDF membranes (Merck Millipore) ...
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bioRxiv - Microbiology 2024Quote: ... protein concentrations were measured using the BCA assay and 20 μg of proteins from whole cell lysates were resolved on pre-cast 12% tris-glycine PAGE (Bio-Rad) followed by transfer to a LF-PVDF membrane using a Bio-Rad semi dry transfer apparatus ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Treated whole cell extracts (5 µg per lane) were resolved on a 12% TGX Stain-free polyacrylamide gel (Bio-Rad, #1610185) by applying 180V until loading dye ran off the gel ...
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bioRxiv - Physiology 2024Quote: ... The proteins were subsequently separated via electrophoresis using 12% SDS-PAGE gels and transferred onto a PVDF membrane (Trans-Blot Turbo, Bio-Rad). S-nitrosylated Cx43 proteins were detected using a mouse anti-connexin-43 antibody (#C8093 ...
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bioRxiv - Genomics 2024Quote: ... Proteins were extracted as described previously,53 and separated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad). Transfer to the membrane was performed with iBind Western System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Equal amounts of bone marrow cell lysates underwent electrophoresis on a gradient SDS-PAGE gel (Criterion XT precast gel, 4-12%, BioRad, 3450123). Proteins were transferred onto nitrocellulose membranes and after blocking with Intercept (TBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... the samples were analyzed by western blotting using equal protein amounts of cell lysates separated by SDS–PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Equal amounts of samples were run on SDS PAGE gels (BIO-RAD 4-12% gradient Bis-Tris or XT Precast gels with catalog numbers mples were run on SDS PAGE gels (BIO-RAD 4-12% gradient Bis-Tris or XT Precast gels with catalog numbers #3450123 or #3450124 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µL of the cleared lysates and 10 µL of washed beads after pull-down were applied on separate Mini-PROTEAN TGX 4-12% polyacrylamide gels (BIO-RAD), and run for 45 min at 200 V in Tris/Glycine/SDS buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Multiplex 24 μL ddPCR reactions were prepared by mixing 12 μL of ddPCR supermix (no dUTP; Bio-Rad, catalog no. 1863024), forward and reverse primers for target and reference genes (IDT ...
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bioRxiv - Genetics 2024Quote: ... Samples were loaded at 15-20 µg per well onto a NuPAGE 4-12% Bis-Tris Acrylamide midi gel along with Precision Plus dual color ladder (BioRad, 1610374). The gel was run on an XCell4 Surelock Midi-Cell (ThermoFisher WR0100 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were analyzed on either a 12% Bis Tris PAGE gel (for histone analytes) or a 4-12% Bis Tris PAGE gel (for all other analytes) using 1x MES running buffer (Bio-Rad) at 180 V ...
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bioRxiv - Plant Biology 2020Quote: Forward and reverse new PCR primers for the 12 Zn transport-related genes and the two reference genes suitable for SYBR® Green II RT-qPCR assays (Biorad, USA) were designed (Table 2) ...
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bioRxiv - Molecular Biology 2020Quote: Protein lysates were separated on 12% SDS polyacrylamide gels and transferred to PVDF membranes using the Trans-Blot Turbo System (BioRad, Feldkirchen, Germany). Equal protein loading and successful blotting was verified by Coomassie Brilliant Blue staining ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein per lysate were loaded onto Criterion XT 4-12% Bis-Tris polyacrylamide gels (Bio-Rad, Hercules, CA), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total cell proteins were run in a 12% SDS-polyacrylamide gel and blotted to the PVDF membrane (Bio-Rad, USA). Membranes were incubated overnight at 4 °C with mature BDNF primary antibody (ThermoFisher OSB00017W ...
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bioRxiv - Molecular Biology 2021Quote: ... for the experiments done on 12-well plate) for each sample on SDS-PAGE using 4–20% Mini-PROTEAN® TGX gels (Bio-Rad), and immunoblotted as described (36) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and MgCl2 [12.5 mM]) and annealed by cooling the solution from 95 to 12 °C over 30 min in a thermal cycler (Bio-Rad, T100). Detailed descriptions for the assembly of individual gate complexes are described in the supplementary information ...
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were resolved by SDS-PAGE 12% self-cast gel and transferred onto polyvinylidene difluoride (PVDF) membrane using the Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) in transfer buffer (25 mM Tris ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... Proteins were separated on SDS-containing 8 to 12 % polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories, Inc., Hercules, USA). After staining with primary and secondary antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... The treated lysates were mixed with the 2 × SDS-PAGE sample buffer with or without 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were then transferred on a Nitrocellulose membrane for 12 minutes using the semi-dry turbo transfer western blot apparatus (Bio-Rad, USA). Post-transfer ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... 20μg of protein were loaded into each lane of a Criterion™ XT Bis-Tris Precast Gel 4-12% (Bio-Rad, UK) for SDS-Page separation ...
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bioRxiv - Microbiology 2020Quote: ... A total of 2.5-10 µg of each sample was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) through 12% Mini-PROTEAN® TGX™ Precast Gels (4561043, Biorad). After transferring the proteins onto a 0.2 µm Nitrocellulose membrane (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were loaded on 4-12% Bis-Tris gel and separated by SDS-PAGE on the Criterion system (Bio-Rad Laboratories), and electrophoretically transferred to nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were run on denaturing sodium dodecylsulfate-polyacrylamide gel electrophoresis gels (12%) before transferring onto Immuno-Blot™ polyvinylidene fluoride membranes (Bio-Rad). The reference protein β-actin was used as a loading control.
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bioRxiv - Cell Biology 2022Quote: ... equal amounts of lysate were loaded into wells of 12% Mini-PROTEAN® TGX 4-20% pre-cast protein gel (BIO-RAD) installed in Mini-PROTEAN® tetra vertical electrophoresis cell filled with 1X TG-SDS solution (Euromedex) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were denaturated at 95°C for 5 minutes before separation on SDS-PAGE TGX 12% Stain-Free FastCast Acrylamide (Bio-Rad Laboratories). Proteins were then electro-transferred onto Trans Blot Turbo polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2021Quote: ... Equal total protein was loaded from each of the indicated cell lysates and run on a Criterion™ XT 4-12% Bis-Tris protein gel (Bio-Rad). Protein was transferred at 4°C to nitrocellulose membrane for 2 hours at 50V ...
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bioRxiv - Microbiology 2020Quote: ... using AnykD or 12% polyacrylamide gels and transferred to PVDF membranes using the Transblot Turbo apparatus (Bio-Rad, Hercules, CA, United States). PVDF membranes were blocked for 1-hour in TBST with 5% milk ...
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bioRxiv - Genomics 2020Quote: ... 5 µL of cDNA was pre-amplified in a 12-cycle PCR reaction using the Sso Advanced PreAmp Supermix (Bio-Rad, USA) in a 50 µL reaction ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
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bioRxiv - Developmental Biology 2022Quote: ... lieberkuehni developmental stages were mixed in 2x Laemmli sample buffer with 10 % 2-β-mercaptoethanol and heated at 97 °C for 5 min before loading on a 12 % (w/v) TGX FastCast SDS/PAGE gel (Bio-Rad, USA). Membranes were blocked in 3 % (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... 40 mg of total protein was loaded onto a 12-15% polyacrylamide gradient gel (Mini-PROTEAN TGX Precast Mini Gel; Bio-Rad Laboratories) and transferred to a nitrocellulose membrane following separation ...
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bioRxiv - Zoology 2020Quote: ... An equal volume of each lysate was added to the designated lane on a 12% polyacrylamide gel (Bio-Rad, Hercules, CA, USA) and separated by electrophoresis ...
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bioRxiv - Plant Biology 2020Quote: Sample loading buffer was added and equivalent amounts of protein (~ 30 μg) separated by SDS-PAGE (1.0 mm thick 4 to 12% polyacrylamide Criterion Bis-Tris XT-gels, Bio-Rad or equivalent) in MOPS buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 μl, BioRad). Before loading ...