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Citations for Bio-Rad :
801 - 850 of 1185 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Each sample (15 μg) was boiled for 5 min and applied on NuPAGE 4%– 12% Bis-Tris Gel (Biorad). The gel was transferred onto a nitrocellulose membrane ...
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bioRxiv - Biophysics 2024Quote: ... the samples were separated by 8% or 12% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Bio-Rad). After blocking with 5% skim milk ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated by electrophoresis using 4-12% Bis-Tris mini protein gels and transferred to PVDF membranes (BioRad). Blots were incubated with primary antibodies at 4°C and then with HRP conjugated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... the lysates were electrophoretically separated by 12% SDS-PAGE gel and transferred to a PVDF membrane (Bio-Rad, #10026934). The proteins were blocked with 5% skim milk (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... protein samples were separated on standard SDS-PAGE (12% acrylamide) gel and subsequently transferred to PVDF membrane (Bio-Rad) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated on Criterion™ XT Bis-Tris 4–12% Protein Gels (Biorad; 345-0124 or 345-0125) in MOPS Running Buffer (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated by SDS 12% PAGE and transferred to polyvinylidene fluoride membranes (Bio-Rad, Marnes-la-Coquette, France) blocked with 5% bovine serum albumin ...
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bioRxiv - Cancer Biology 2024Quote: An equal amount of total protein was resolved on precast 4%–12% SDS-PAGE gels (Biorad, Hercules, CA, USA), and the protein was transferred onto PVDF membranes as previously described 2 ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were separated on 12% SDS-PAGE polyacrylamide gels and transferred to Polyvinylidene fluoride (PVDF) membranes (Bio-Rad Laboratories). The membranes were blocked using a blocking buffer Tris-buffered saline containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Cell Biology 2024Quote: ... Migration of proteins lysate was performed in 4-12 % gradient or in 10 % SDS-PAGE gels (Bio-Rad Laboratories), transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted from SSLs using 350 μL of RIPA buffer and was purified using the Ready Prep 2-D cleanup kit (Bio-Rad, Hercules, CA, USA); the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Blocking of the membrane was carried out by incubating in blocking solution for 1 h at room temperature (5% Bio-Rad Blotting-Grade Blocker in TSB-T buffer). Membrane was then incubated with anti-PcdA polyclonal antibody diluted 1:15,000 in blocking solution for 2 h at room temperature.
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bioRxiv - Biophysics 2020Quote: ... proteins from the supernatant were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amount of proteins was loaded onto 12% SDS-PAGE gel (acrylamide: bisacrylamide 29:1, cat# 161-0146, Bio-Rad) and after size separation blotted onto 0.2 μm nitrocellulose membrane (cat# 162-0112 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were eluted in SDS sample buffer and 30 μl of each sample was run on a 12% Bis-Tris 1D SDS-PAGE gel (Biorad) for 1 cm and stained with colloidal Coomassie dye G-250 (Gel Code Blue Stain Reagent ...
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bioRxiv - Molecular Biology 2020Quote: ... About 80 µg of denatured proteins were loaded for each condition and separated on gradient 4–12% polyacrylamide classic or TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitates were resolved on 4-12% Criterion XT Bis-Tris or 4-20% Mini-Protean TGX precast gels (BioRAD) and transferred to Immobilon-FL PVDF membranes (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated by SDS-PAGE on 12% polyacrylamide gels and transferred to a Hybond ECL membrane (Amersham biosciences) using a semi-dry transfer system (Biorad). Membranes were subsequently blocked by incubation for 1 h at room temperature in 5 % (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... 30 µg of total protein per sample was loaded on NuPage 4-12% Bis-Tris Protein gels (Thermo Fischer Scientific) or 4–15% Mini-PROTEAN TGX StainFree Protein gels (Biorad) and run at 200 V as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... supernatants aspirated and loaded on to 4-12% NuPage Bis/Tris gels for separation before transfer on to a Nitrocellulose membrane (Biorad) at 4°C at a constant current of 350mA in transfer buffer (25 mM Tris ...
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bioRxiv - Synthetic Biology 2020Quote: ... 25μl of each sample was loaded onto a 12% SDS PAGE gel and then transferred to 0.45 mm nitrocellulose membranes (Bio-rad). Protein loading was assessed through ponceau staining of nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell extracts were prepared using UREA lysis buffer and 30-60μg of total protein was loaded on a 7.5% or 12% denaturing polyacrylamide self-cast gel (Bio-Rad, Switzerland). Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074 ...
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bioRxiv - Neuroscience 2020Quote: ... and boiled at 95°C for 5 min before loaded onto a 4-12% SDS/PAGE gel and run at 200 V before electrotransferred to a membrane using Transblot-Turbo Transfer system (BioRad). The membrane was then washed 2 × 15 min in TBS with 0.1% Tween20 (TBST) ...
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bioRxiv - Bioengineering 2021Quote: Protein samples were mixed with Laemmli buffer and the proteins were separated by SDS-PAGE using either a 7.5 % or a 12 % polyacrylamide gel and a Tris-glycine running buffer (Bio-Rad). The Precision Plus Protein™ Unstained Standard (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... respectively 45 μL and 10 μL of proteins were loaded for each condition and separated on gradient 4-12% polyacrylamide TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Around 40 µg of proteins were loaded per lane of a 4-12% precast gel (Bio-Rad Mini-PROTEAN®). Proteins were transferred on PVDF-membrane and blocked for 1h with 5% skimmed milk ...
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bioRxiv - Neuroscience 2021Quote: ... and the volumes corresponding to 35 μg were loaded onto TGX Stain-Free FastCast 12% polyacrylamide gels (Bio-Rad, USA) for electrophoretic separation ...
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bioRxiv - Neuroscience 2021Quote: Samples were resolved by SDS-PAGE using either 10 or 12% acrylamide/bis-acrylamide gels and transferred to 0.22 µm nitrocellulose (Bio-Rad). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 12 μg of total protein were loaded onto a 4-20% mini Protean TGX gel (Bio-Rad, Hercules, CA) and blots were evaluated as described previously
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bioRxiv - Plant Biology 2021Quote: ... Total proteins were separated by electrophoresis in 12 % SDS-polyacrylamide gel and electrophoretically transferred to a polyvinylidene fluoride membrane according to the manufacturer’s instructions (Bio-Rad). Transferred proteins were detected with Ponceau-S ...
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bioRxiv - Neuroscience 2021Quote: ... self-cast 10% or 12% SDS-gels or Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). After electrophoretic separation ...
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bioRxiv - Physiology 2021Quote: ... About 30 μg of protein from the sample extracts were electrophoresed in 12% Mini-PROTEAN TGXTM precast gels (Bio-Rad) using the electrophoresis system mini-PROTEAN Tetra Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were run on 12% polyacrylamide gel and transferred to a nitrocellulose membrane using the Trans-blot SD semi-dry transfer cell (Biorad). To represent the same number of cells in each lane ...
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bioRxiv - Molecular Biology 2020Quote: ... Running buffer was prepared with tris-glycine 1X and SDS 0.1% using a homemade gel (7-12% bis-acrylamide, Bio-Rad), which ran at 100 V for approximately 2:30 h ...
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bioRxiv - Microbiology 2020Quote: ... Samples were run in 3-12% Bis-Tris gradient gels and transferred to PVDF membranes in a wet blot system (BioRad). Membranes were dried ...
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bioRxiv - Cell Biology 2021Quote: ... separated by SDS-PAGE (12% T, 1 mm, 200 V; BioRad mini protean III cell; BioRad Laboratories GmbH, München, Germany) and stained with Coomassie Brilliant Blue G-250.
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bioRxiv - Immunology 2022Quote: ... Total of 15-30µg protein were separated on a 4-12% Bis-Tris SDS PAGE and transferred using BioRad Turbo Blot (1704156, BioRad) onto PVDF membrane ...
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bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
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bioRxiv - Physiology 2022Quote: ... and was and then electrophoretically separated at 240V for 22 minutes on a 7-12% TGX gradient gel (BioRad, 4568081). Proteins were then transferred to a PVDF membrane using a BioRad Trans Blot Turbo ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was separated by SDS-PAGE using 12% acrylamide Mini-protean TGX precast gels (Bio-Rad, USA). Gels were stained with Coomassie (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 60 μg of protein was separated on 10% SDS/PAGE (Mini-PROTEAN TGX Gels,12%,10-well, 4561043, BIO-RAD) and transferred onto nitrocellulose membrane (#1620115 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein samples were loaded on 10%-12% polyacrylamide gels and transferred onto 0.45 µm pore-size nitrocellulose membranes (Bio-Rad) using the Turboblot (BioRad) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracted from tobacco or kiwifruit were separated on 12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad) using a highly efficient wet protein transfer system (eBlot L1 ...
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... Samples were run on a precasted gradient gel (4 – 15% Criterion TGX, 12+2 wells, 45 μl, BioRad, CA, USA) for 45 min at 60 mA ...
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bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Molecular Biology 2020Quote: ... and adjusted before isolation of protein aggregates as well as verified by running reduced samples on Criterion XT Precast Gel 4-12% Bis-Tris (BioRad) and staining with Coomassie Brilliant Blue R-250 staining solution (BioRad) ...
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bioRxiv - Physiology 2021Quote: ... Proteins (50 μg) were loaded onto 12–15% SDS-PAGE gels using a Mini-Protean apparatus (Bio-Rad, CA, USA) and transferred onto nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... An equal amount of total protein (30μg) were resolved on precast 4-12% SDS-PAGE gels (Biorad, Hercules, CA, USA), transferred onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: Samples were prepared for standard SDS-PAGE and separated on 12% acrylamide gel (Mini-PROTEAN TGX precast gels, Bio-Rad), then transferred onto 0.2 µm pore size PVDF membranes (Millipore ...