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Citations for Bio-Rad :
1001 - 1050 of 5928 citations for 6 hydroxy 4 6 dihydrofuro 3 2 c pyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
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bioRxiv - Microbiology 2020Quote: ... The samples were heated at 90°C for 10 minutes and loaded onto a 4-20% precast polyacrylamide gel (BioRad). The gel was stained with SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Purified TREK-2 was then mixed with GUVs to a final concentration of ∼1 - 5 µg/ml and incubated overnight at 4 °C with 0.5mg/ml Bio-Beads (Bio-Rad) prior to use.
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bioRxiv - Genomics 2019Quote: ... at 4 °C and transferred to a nitrocellulose membrane (0.45 μM) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current of 0.12 A for 1 □ hour ...
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min at 100°C in SDS-PAGE sample buffer and separated on Tris-glycine 4-20% SDS-PAGE gels (BioRad). Detected protein bands for each blot were quantified using ImageJ software for gel densitometry measurements of NSP4:GAPDH.
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bioRxiv - Microbiology 2019Quote: ... The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences) for 2h at 4°C using a disposable chromatography column (BioRad). The IgG-Sepharose column was washed with 35 ml of protein A binding buffer (10 mM Tris–HCl ...
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bioRxiv - Physiology 2019Quote: ... Protein lysates were prepared by centrifugation at 15,000g for 10min at 4°C and protein concentrations were calculated using Bradford assay (BioRad, UK) with BSA as a standard ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation at 10,000xg for 10 minutes at 4°C and then boiled in 2x Laemmli Sample Buffer (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared for 10 min at 14000 rpm at 4°C and protein concentration of the supernatant was determined by using Protein Assay (BioRad). The co-immunoprecipitation was performed using GFP trap® beads (Chromotek ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 14,000 rcf for 20 min at 4°C and protein amounts in the supernatant determined using the Bradford assay (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged at 14,000g for 15 min at 4°C and supernatant protein concentrations were determined by Bradford assay (Bio-Rad).
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
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bioRxiv - Neuroscience 2022Quote: ... Regional lysates were centrifuged at 21,000 x g for 20 minutes at 4 °C and the supernatant was removed and suspended in 4X laemmli buffer (BioRad) with 2-mercaptaethanol (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE then transferred to nitrocellulose membrane at 4°C (0.45 μm pore size, Bio-Rad). Membranes were blocked overnight at 4° in Intercept tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were cleared by centrifugation at 14,000 g for 15 min at 4°C and protein concentration was determined using the Bradford assay (Bio-Rad). Sodium dodecyl sulfate (SDS ...
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bioRxiv - Molecular Biology 2023Quote: ... Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad). The loaded resin was then washed 3-4 times with 6 ml Wash Buffer (50 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2023Quote: ... Obtained samples were denatured in a 1xLaemmli buffer at 95°C for 10 min and proteins separated using electrophoresis on a 4-15% mini-protean-TGX-precast-gel (BioRad). Proteins were then transferred on nitrocellulose membranes by a semi-dry method using transblot turbo transfer system (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were stained with primary antibody in blocking buffer at 4°C overnight: anti-NP (1:5000, OBT1555, Bio-Rad), anti-PA ...
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bioRxiv - Cancer Biology 2023Quote: ... held at 4 °C) was performed in a C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad). After RT reaction ...
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bioRxiv - Biochemistry 2023Quote: ... Blue Native PAGE gel experiments were performed in a cold room (4 °C) using 10% precast gels (BioRad Mini-PROTEAN) and run at 170 V for 55 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... or poly-acrylamide gels and proteins were transferred overnight at 4°C on a nitrocellulose membrane using a liquid transfer system (BioRad). Non-specific sites were blocked with 5% non-fat dry milk in PBS 0.1% Tween 20 ...
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bioRxiv - Microbiology 2023Quote: Protein samples were centrifuged (150,000 × g for 10 min at 4°C) and loaded onto an Enrich SEC 650 10 × 300 column (Bio-Rad) using the AKTA explore system (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled 5min at 90°C and 10µl was loaded on a 4-20% gradient SDS-PAGE gel (Biorad #4561096). Gel was stained with Coomassie brilliant blue R.
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Molecular Biology 2024Quote: ... 95 °C) and 1.25 µg sample was resolved on a 4-12% acrylamide Criterion XT Bis-Tris gel (Bio-Rad) in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
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bioRxiv - Molecular Biology 2024Quote: ... and heat denatured at 95°C for 10 min before loading onto a 4-15% Tris-glycine gel (Bio-Rad) and electrophoresed at 125 V ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured for 10 minutes at 70°C and proteins were separated (55min, 200V) on a Midi 4-12% Bis-Tris gel (BioRad) in 1x MOPS buffer (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... eluate was incubated at 70 °C then run on 4-12% NuPAGE gell and transferred to LF PVDF membrane (BioRad). Western-blot analysis were done with the next antibodies ...
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bioRxiv - Microbiology 2024Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 hr at 4°C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% non-fat dry milk and incubated with primary antibodies overnight at 4 °C and then incubated with host species-matched HRP-conjugated secondary antibodies (BioRad) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: 96-well flat-bottom NUNC Maxisorp plates were coated overnight at 4 °C with 0.5 mg/mL recombinant HBsAg (BIO-RAD). Plates were washed with PBS/T ...
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bioRxiv - Biochemistry 2024Quote: ... denatured for 10 min at 96°C before it was separated at a 4-20 % PROTEAN TGX stain-free gel (BioRad) at 200 V for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... One-step RT-qPCR was performed using an iTaq Universal SYBR Green One-Step RT-qPCR Kit (BIO-RAD). The reaction was performed in a total volume of 20 μL by mixing 10 μL iTaq universal SYBR Green reaction ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 μg of eluted RNA was used for cDNA synthesis employing iScript (Bio-Rad). Samples were analyzed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: Cell lysates were harvested by directly lysing the cells with 2 × sampling buffer (Bio-Rad). Proteins were separated by SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 x 106 cKit enriched cells were resuspended in 150 µl Laemmli buffer (BioRad 1610737) supplemented with 1:10 β-mercaptoethanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The amplified samples were run and cut from 2% low-melt agarose gels (Bio-Rad) and followed by sRNA sequencing with NextSeq500 (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rat Ly-6B.2 alloantigen (1:100, MCA771GA, Bio-Rad Laboratories, Hercules, CA) for neutrophils ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted fraction was precipitated with Ready Prep 2-D cleanup kit (Bio-Rad, USA). The precipitate was dissolved in O’Farrell lysis buffer and analyzed with 2D polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2019Quote: ... with a 5μl reaction mixture containing 2.5μl 2× SYBR Green MasterMix (Bio-Rad, Hercules, USA), 0.25μL of both 10μM forward and reverse primers and 2μl cDNA (5ng/μl) ...
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Interaction of a sarcolipin pentamer and monomer with the sarcoplasmic reticulum calcium pump, SERCAbioRxiv - Biophysics 2019Quote: ... Detergent was slowly removed by the addition of SM-2 biobeads (Bio-Rad, Hercules, CA) over a 4-hour time course (final ratio of 25 biobeads ...