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Citations for Bio-Rad :
951 - 1000 of 8354 citations for 7 8 DIHYDRO 1 3 DIOXOLO 4 5 G ISOQUINOLIN 5 6H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured at 95 °C for 5 min in Laemmli sample buffer (Bio-Rad) containing 2.5% v/v 2-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... in 5% milk at room temperature and imaged using ChemiDoc MP Imaging System (Bio-Rad). Antibodies used for immunoblotting can be found below ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with blocking solution consisting of 5% Blotting-Grade Blocker (Bio-Rad, #1706404) in TBST (20mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Ten nanograms of genomic DNA was added to each qPCR reaction (5 µL of BioRad SYBR green 2X master Mix ...
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bioRxiv - Immunology 2024Quote: ... before being blocked in 5% (w/v) skim milk with 0.2% Tween 20 (Bio-Rad). Loading was additionally controlled by detecting GAPDH as a control (Anti GAPDH antibody sourced from abcam ...
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bioRxiv - Cell Biology 2024Quote: 2-10ug of RNA extractions were loaded into a 5% acrylamide (Bio-Rad Laboratories: 1610156), 0.5x TBE (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Non-phospho-specific antibodies were diluted in TBST/ 5% non-fat dry milk powder (BioRad). Phospho-Rab10 levels were determined by the site-specific rabbit monoclonal antibody anti-pRAB10(pT73 ...
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bioRxiv - Microbiology 2023Quote: ... The reactions were incubated at 65 °C for 5 min in a thermocycler (Bio-Rad) and chilled on ice for 5 min ...
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bioRxiv - Pathology 2021Quote: ... We performed one-step multiplex RT-qPCR using the Reliance One-step Multiplex Supermix (Bio-Rad), PrimePCR Probe Assays (FAP-FAM500 ...
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bioRxiv - Immunology 2020Quote: ... mixed with Rotiload (1:4, BIO-RAD, Feldkirchen, Germany) and boiled for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... diluted 1:4 in Laemmli sample buffer (Bio-Rad) and heated for 10 minutes at 98°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The products were analyzed by 8% acrylamide/bis-acrylamide (19:1 ratio, Bio-Rad, 1610145) gel containing 1x TBE and 7M Urea ...
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bioRxiv - Immunology 2023Quote: ... the membranes were incubated with protein G-HRP conjugate (1:3000, Bio-Rad) for 1 hr at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... running Quantity One software (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: Quantity One Software (Bio-Rad Laboratories) was used to quantify immunoblots and TLC plates ...
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bioRxiv - Plant Biology 2021Quote: ... and Quantity One software (Bio-Rad). Quantity One software was also used to quantify atpT and 5S rRNA transcript levels ...
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bioRxiv - Cell Biology 2020Quote: ... one pulse by GenePulser electroporator (BioRad).
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bioRxiv - Genetics 2022Quote: ... and Quantity One software (Bio-Rad) respectively.
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bioRxiv - Neuroscience 2022Quote: ... with Quantity One software (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... Quantity One version 4.6.2 (Bio-Rad) was used to quantify each band and then convert the graphical results into digital results ...
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bioRxiv - Genomics 2023Quote: ... One 0.1cm cuvette (Bio-Rad, 1652089) for each transformation and clean DNA in water were prepared on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... 15µg of each sample was loaded per lane of a MIDI 4-15% Criterion Precast Midi SDS page gel (BioRad #5671085) and electrophoresis was performed at 120mV for 75 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... and the equivalent of 50 μg protein input (elution) were analyzed on a 4%-12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of total protein was run on a 4–15% SDS-PAGE gel (Mini-PROTEAN TGX Precast Protein Gel; Bio-Rad). Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Pathology 2021Quote: ... maximum power with 30 second intervals and the lysate obtained by ultracentrifugation at 100,000 g for 60 minutes, 4°C (CS 100FNX, HITACHI) resuspended in PBS and the concentration was measured by Bio-Rad protein assay dye reagent concentrate (BioRad) ...
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bioRxiv - Cancer Biology 2020Quote: ... 30 μg of protein was loaded into each lane of a TGX-stain-free pre-cast 4–20% SDS gel (Biorad, 4568094), total protein was quantified upon stain-free gel imaging ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected after centrifugation at 15,000 ×g for 15 min at 4°C and applied to a 20 mL open column (Poly-Prep Chromatography Columns; BIO-RAD) containing 500 µL of Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were then clarified through centrifugation (14’000 g, 20 minutes, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were centrifuged at 20000 g for 20 min at 4 °C and then filtered by gravity using Poly-Prep chromatography columns (731-1550, Bio-Rad). Supernatants were filtered by gravity through Poly-Prep chromatography columns (731-1550 ...
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bioRxiv - Microbiology 2020Quote: ... 1μg of pKSB vector containing aimed mutation and the bacterial liquid were mixed at 4℃ and electroporated by Micropulser (Bio-Rad) with Ec2 (2.5kV ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10 to 25 μg for experiments that were imaged digitally) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run either at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were cleared by centrifugation (10 min at 14,000 x g, 4°C) and quantified by Bradford (Bio-Rad, cat#5000205). All samples were then boiled for 5 min in Laemmli sample buffer ...
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bioRxiv - Neuroscience 2022Quote: ... A GS-800 calibrated densitometer with Quantity One 1-D Analysis Software 4.6 (Bio-Rad Laboratories) was used for quantitative analysis of protein levels ...
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bioRxiv - Microbiology 2023Quote: ... one-step RT-ddPCR (1-Step RT-ddPCR Advanced Kit for Probes, Bio-Rad, Hercules, CA) was used to determine absolute quantification of N and S vRNA copy numbers in culture supernatants and cell lysates using the OC43 ...
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bioRxiv - Developmental Biology 2020Quote: ... and one-dimensional electrophoresis was carried out for 4 hours at 75-100 volts in a Tris-glycine-SDS (Bio-Rad, catalog 161-0732) running buffer ...
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bioRxiv - Microbiology 2020Quote: ... Maltose-1-phosphate was separated from orthophosphate by anion exchange chromatography using Dowex 1 × 8 100-200 mesh (Bio-Rad, 28 x 1.6 cm ...
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bioRxiv - Microbiology 2020Quote: ... one-step RT-qPCR was performed using the 4X Reliance One-Step Multiplex RT-qPCR Supermix (BioRad), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... including the one-step reverse transcription (One-Step RT-ddPCR Advanced Kit for Probes, Bio-Rad Laboratories) and the triplex probe assay for PCR amplification (2019-nCoV CDC ddPCR Triplex Probe Assay ...
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bioRxiv - Molecular Biology 2020Quote: ... A one-step reaction mix was prepared using Reliance One-Step Multiplex Supermix (BioRad Cat. No. 12010220) with 2 ul of sample ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 g SDS in 1 L distilled water) using the Mini-PROTEAN® Tetra electrophoresis system (Bio-Rad). Then ...
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bioRxiv - Microbiology 2019Quote: ... and 3 mg Bromophenol Blue) were separated by SDS-PAGE using 4-20% gradient polyacrylamide gels (Bio-Rad Laboratories) at 10 mAmps for 16 h ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed once with PBS for 10 min followed by a blocking- permeabilization step for 1 hr at RT in blocking buffer (PBS/ 5% donkey serum Bio-Rad C06SB / 0.1% Triton Sigma X100). Primary antibodies diluted in blocking buffer were then added to the cells (Table 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 μL of DNAse (1:3 in DNase buffer) (Biorad, 10042051) was added to 700 ng of RNA sample ...
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bioRxiv - Neuroscience 2023Quote: ... mixed 3:1 with 4x Laemmli buffer (Biorad, cat. no. 1610747) containing β-mercaptoethanol and heated at 95°C for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µL of DNAse (1:3 in DNase buffer) (Biorad, 10042051) was added to 700 ng of RNA sample ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by electroporation with 5 ms pulses of 1,350 V using the MicroPulserTM (BioRad). Cells were then resuspended in LB liquid (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Individual DNA was extracted in 200 μL 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA) [36] ...