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Citations for Bio-Rad :
1201 - 1250 of 8354 citations for 7 8 DIHYDRO 1 3 DIOXOLO 4 5 G ISOQUINOLIN 5 6H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Oligo-dT first strand cDNA were synthesized from 5 μg total RNA by iScriptAdv cDNA kit (Biorad, # 1725038) following the manufacturers recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were further denatured at 80 °C for 5 minutes and loaded in 12% acrylamide gels (Bio-Rad). Electrophoresis was carried in Tris-Acetate SDS running buffer (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... and the PVDF membrane was blocked in 5% non-fat dry milk-based blocking-grade blocker (Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was extracted using 250 μL of 5% Chelex® 100 Resin (Bio-Rad laboratories, Hercules CA) and 3 μL of Proteinase K (20 mg/ mL ...
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bioRxiv - Synthetic Biology 2019Quote: ... Samples were vortexed a 5 µL was loaded onto a mini-protean TGX stain free gel (Biorad, 4568095) alongside 5 µL of Precision Plus ladder (Biorad ...
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bioRxiv - Developmental Biology 2020Quote: ... boiled 5 min and separated by SDS-PAGE on Mini-PROTEAN® TGX™ precast gels (Bio-Rad). Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal ...
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bioRxiv - Genetics 2020Quote: ... Then 5 μL of worms were mixed with 40 μL of electroporation buffer (Gene Pulser Electroporation buffer, Biorad) in 1.5 mL tubes ...
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bioRxiv - Genetics 2020Quote: ... The protein was pulled down by a disposable column loaded with 5 ml Nuvia IMAC resin (BioRad Lab) at 1 ml min-1 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were left on ice for 5 min and then analyzed in a S3e Cell Sorter (Bio-Rad) with a laser excitation at 488 nm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 μl of the cDNA sample were supplemented with 5 μL of SsoADV Universal SYBR Green Supermix (BioRad) mix 2X ...
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bioRxiv - Immunology 2020Quote: ... and transferred onto a PVDF membrane (Thermo Fisher Scientific).The membranes were blocked by 5% skim milk in TBST (Tris-buffered saline /0.1% Tween 20, Bio-Rad) and then incubated with antibodies to collagen a1 (IV ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed 3x for 5 min with TBS-T and developed with Clarity Western ECL (Bio-Rad) per manufacturers guidelines ...
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bioRxiv - Immunology 2022Quote: ... 42 °C for 50 min and 85 °C for 5 min in a thermal cycler (Bio-Rad, USA). Synthesized cDNA was kept at −80 °C till further use ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA was resuspended with Tris-EDTA and 5% Chelex-100 chelating resin (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Microbiology 2022Quote: Each 10 μl PCR reaction consisted of 5 μl of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Germany), 250 nM of forward and reverse primers ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 6 x 5 minutes with TBS-T before developing with Clarity Western ECL Substrate (Bio-rad).
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bioRxiv - Microbiology 2022Quote: ... 0.02% bromophenol) and 5 µL of each sample were loaded onto an AnykD precast polyacrylamide gel (Bio-Rad). The gel was run in 25 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated along with 5 µl Precision Plus Protein All Blue Prestained Protein Standards (Bio-Rad, 1610373) at 80-120 V in SDS-PAGE running buffer containing 25 mM Tris ...
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bioRxiv - Physiology 2023Quote: ... membranes were housed in clear blotting boxes and blocked in 5% blocking buffer in TBS-T (BIORAD 1706404) for 40 min at +25°C ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed three times for 5 min each with TBST and incubated with StarBright B520 (BioRad) as the secondary antibody at 1:2,500 in 5% nonfat dry milk in TBST 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein (5-10 ug) was loaded in 15 well pre-casted gradient gel (456-8086, Bio-Rad). After electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein extracts were loaded and run on 5-20% gradient pre-cast gels (Bio-Rad) before being transferred onto a 0.45 μM nitrocellulose membrane (Amersham ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked with Tris-buffered saline with 0.05% Tween-20 (TBST) containing 5% dried nonfat milk (Biorad). Primary antibodies were diluted in TBST containing 5% bovine serum albumin and 0.02% NaN3 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with 5 μM MitoSOX for 20 minutes and analyzed using ZE5 Cell Analyzer (Bio-rad).
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bioRxiv - Immunology 2023Quote: ... Lysates were heated at 95 °C for 5 min together with Laemmli sample buffer (Bio-Rad, catalog #1610747). Lysates were resolved using a 4% to 15% Criterion TGX Stain-Free Precast gel (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... folic acid 500 μg/L) or on Columbia agar solid support enriched with 5% horse blood (COH) (Biorad). Anoxic conditions were generated in Gaspack (BD ...
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bioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions and an iQ™5 real-time PCR System (Bio-Rad Laboratories, USA). All reactions were pre-denaturation at 95°C for 3 min ...
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bioRxiv - Plant Biology 2023Quote: ... and 5 μL of diluted cDNA was implemented for real-time quantitative PCR using the SYBG reagent (Biorad). The PCR reaction was performed in the thermocycler (C1000 TouchTM Thermal Cycler ...
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bioRxiv - Neuroscience 2023Quote: ... Blocking was performed at room temperature for 60 min with blocking buffer: 5% nonfat dry milk (Bio-Rad) in TBST (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... heated to 60 LJC for 5 min and electrophoresed into a 12% Tris-HCl Criterion gel from BioRad at 50 V for 20 min or until the dye front moved just below the bottom of the sample wells ...
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bioRxiv - Bioengineering 2023Quote: 5 uL of each sample was pipetted into a Hard-Shell 384-well clear microplate (Bio-Rad #HSP3805), and fluorescence and absorbance readings were taken by a Synergy H1 microplate reader (BioTek) ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... Quantitative PCR was performed in 10 µl reaction containing 5 µl SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µl H20 ...
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bioRxiv - Cell Biology 2023Quote: ... and 5-10 μg aliquots fractionated by SDS-PAGE then transferred to nitrocellulose (0.45 μm) membrane (Bio-Rad). Membranes were blocked for 1 h at room temperature in 5% BSA in 1x Tris-buffered saline + 0.1% Tween 20 (TBST) ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with Tris-buffered saline with 0.05% Tween-20 (TBST) containing 5% dried nonfat milk (Biorad). Primary antibodies were diluted in TBS-T containing 5% dry nonfat milk (Biorad) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins were denatured for 5 min at 95 °C and cooled to RT before acrylamide (#1610140, Bio-Rad) was added to a final concentration of 4% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples were boiled for 5 minutes at 95°C in 4x Laemmli Sample Buffer (Bio-Rad, 1610747) and run on SDS-acrylamide gels ...
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bioRxiv - Pathology 2022Quote: ... The expression levels of target proteins were quantified with Quantity One 1-D Analysis Software (Bio-Rad, Hercules, CA) and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Plant Biology 2024Quote: ... The gels were visualised using a UV trans-illuminator Gel Doc 2000 and Quantity One 1-D analysis v4.6.5 software (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... the detergent was removed by adding 0.5 g mL-1 (w/v) Bio-Beads (Bio-Rad) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The purified MCU-EMRE subcomplex in DDM was then reconstituted into nanodisc by incubating with Msp1 protein, lipids (POPC:POPE:POPG, 3:1:1 molar ratio) and BioBeads (BioRad) at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used 1 μL diluted cDNA (1:3 in ddH2O) and iTaq Universal SYBR Green Supermix (Bio-rad) in the Bio-rad CFX96 real-time PCR detection system for qPCR experiments ...
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bioRxiv - Microbiology 2022Quote: ... 7 μL of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and RNase-free water for a final reaction volume of 15 μL in each well ...
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bioRxiv - Developmental Biology 2022Quote: ... 7×8.5 cm precut nitrocellulose membranes (BioRad, cat. #162-0146), at 4°C with magnetic stirring and an opposing cold-pack ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was conducted using the QuantumStudio-7 (Bio-Rad). mRNA expression levels were quantified by real-time PCT using SYBR green fluorescence ...
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bioRxiv - Neuroscience 2024Quote: ... for 7 min using Trans Blot Turbo System (Bio-Rad). Filters were washed three times and blocked for 1 hour in Tris-Tween buffered saline (TTBS ...
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bioRxiv - Neuroscience 2021Quote: ... with primers listed in Table 4-1 on a CFX96 system (BioRad). Thermocycler conditions were 95°C for 3 min followed by 40 cycles of 95°C for 10 sec and 55°C for 30 sec ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to a 1:4 mixture of Laemmli Sample Buffer (BioRad) and 2- Mercaptoethanol (BioRad ...
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bioRxiv - Immunology 2021Quote: ... Recombinant Bovine Interleukin-8 PBP039 and Goat anti Bovine Interleukin-8 Ab conjugated to biotin AHP2817B (all from Bio-Rad, protocol according to the manufacturer’s intructions).
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bioRxiv - Evolutionary Biology 2021Quote: ... Each female was macerated in a 50 μl 5% Chelex (Chelex 100 Resin, Bio-Rad Laboratories, Hercules, CA, USA) solution and 1μl proteinase K ...
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bioRxiv - Developmental Biology 2021Quote: ... The PVDF membrane was blocked for 30 minutes at RT using 5% Blotting-Grade Blocker (170-6404, BIO-RAD)/Tris buffered saline with Tween 20 (TBST ...