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Citations for Bio-Rad :
9451 - 9500 of 9625 citations for Recombinant Human EGFR protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 10-20 µl of the cell lysates were separated on 4-15% Mini-PROTEAN® TGX™ Stain-FreeTM Protein Gels from Bio-Rad and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates from each cell line were analysed on multiple gels (four per cell line) with Precision Plus Protein Dual Color Standard (1610394, Bio-Rad, Hercules, CA) as ladder and blotted to membranes to separately probe for phosphorylated and total forms of the same proteins ...
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bioRxiv - Systems Biology 2024Quote: 25 μg of RIPA or 5ug of lipid raft protein lysate was loaded onto a dot blot apparatus (Bio-Rad, Hercules, CA, USA) and were filtered through 0.45μm PVDF for 2 hours by gravity filtration ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting pellets were resuspended in resuspension buffer (RB; Table S7) and proteins levels were quantified by Lowry (Bio-Rad #5000113, #5000114, #5000114). 600 µg aliquots of each sample were pre-heated at 95°C for 5 min ...
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bioRxiv - Pathology 2024Quote: ... 15 µg of proteins were resolved on Criterion TGX Stain-Free 4-15%,18 and 26-well gel (Bio-Rad Laboratories, #5678084, #5678085) in a Criterion large format electrophoresis cell (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2024Quote: ... Then we separated proteins into 4-15% gradient SDS-PAGE gels and transferred them to PVDF immuno-blot membranes (Bio-Rad, Hercules, CA). The primary antibodies S6 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The relative amount of transferred protein per sample was determined after Ponceau S staining by a GS-800 densitometer (Bio-Rad, Hercules, USA) and used as loading control ...
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bioRxiv - Cell Biology 2023Quote: ... Total nascent peptides were detected by in-gel imaging at 647 µm and total protein was detected by far red epi imaging after staining with 0.1% Coomassie brilliant blue (BioRad ChemiDoc MP Imaging System). For detection of nascent insulin and tubulin ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times with TBS-Tween 0.2 % for 15 min and immunoreactive proteins were detected with enhanced chemiluminescence reagents according to the manufacturer instructions (Bio-Rad, Hercules, CA USA). Signals were captured with the ChemiDoc system (Bio-Rad ...
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bioRxiv - Pathology 2023Quote: ... The total protein concentrations of each liver samples were determine using a standard curve generated with BSA at different known concentration using the Quick Start™ Bradford Protein Assay (Bio-Rad, U.S.A.). On the basis of the measure protein cencetrions ...
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bioRxiv - Cell Biology 2023Quote: ... then centrifuged at 13,000 rpm for 30 min at 4 °C and the resulting supernatants were subjected to Bradford protein assay (Bio-Rad, Hercules, California, USA) for measuring total protein concentration ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µL of protein lysate was then loaded into 4–20% Criterion™ TGX Stain-Free™ precast gels (Bio-Rad, Cat. #5678094). Proteins were separated at 200V for 45 min and then transferred to a low-fluorescence PVDF membrane at 25V for 30 min using the Trans-Blot Turbo Transfer System and Kit (Bio-Rad ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... ECL signals and total protein loading amounts were measured with a Bio-Rad ChemiDoc MP imaging system (Bio-Rad Laboratories Inc., Hercules, CA). The acquired images were analyzed with Bio-Rad ImageLab software version 6.1 (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... Electrophoresis and blotting of protein extracts were performed using mini-PROTEAN tetra handcast and trans-blot turbo transfer systems (Bio-Rad, Hercules, CA).
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bioRxiv - Immunology 2023Quote: ... Same protein amounts were loaded onto 4-12% NuPAGE Gels and protein was transferred to PVDF membranes by using Trans-Blot® Turbo™ Transfer System (Biorad#17001917). Membrane was blocked with 5% milk-TBS ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μg protein were separated by SDS-PAGE on 4-15% Mini- PROTEAN® TGX Stain-Free™ Gels (Bio-Rad®). and transferred onto PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad®) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the concentrated AmGSTD1 protein was injected onto a Hydroxyapatite column (HA) connected to the NGC chromatography System (Bio-Rad Laboratories, Hercules, CA, USA). A gradient of 5 mM NaPi and 5 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein contents were visualized on a ChemiDoc MP system using the ImageLab software for quantification (both from Bio-Rad Laboratories GmbH, Munich, Germany). For the demonstration of equal loading ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad, Cat #: 1704158EDU) on a Trans-Blot Turbo TM Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: Some of the affinity chromatography-purified proteins were run through a Superdex200 Increase 10/300 GL column integrated in an NGC chromatography system (Bio-Rad, CA, USA) to obtain a profile of the content in different species (aggregates ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal quantity of the protein was resolved in a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred to an Immun-Blot PVDF Membrane (Bio-Rad) for antibody incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... at room temperature for 1 h with slight shaking and SP-A-S protein or SP-A-RBD complexes were detected by adding HRP-conjugated Goat Anti-rabbit IgG (1:2000, Bio-rad, Hercules, USA) for an additional 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 5 min at 95°C and loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Germany). Proteins were transferred on nitrocellulose membranes and membranes were blocked with 5% BSA in PBS-Tween ...
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bioRxiv - Molecular Biology 2023Quote: 20-25μg of protein lysate with 4x Laemmli buffer (cat# 1610747; Bio-Rad Laboratories was separated on 4-15% Mini-PROTEAN TGX Precast Protein Gels (cat# 4561086; Bio-Rad Laboratories) and transferred to Immun-Blot PVDF Membranes for Protein Blotting (cat# 1620177 ...
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bioRxiv - Physiology 2023Quote: ... 25 µg of total protein was loaded into precast 10 % BIO-RAD Mini-PROTEAN® TGX Stain-FreeTM SDS-polyacrylamide gel (Bio-RAD Laboratories), then electrophoretically transferred to 0.2 µm nitrocellulose membrane (Trans-Blot® TurboTM Transfer Pack ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein transferred to 0.45 mm polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo transfer system (BioRad Laboratories, Hatfield, PA, USA).
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were first analysed using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) using TGX Stain-Free precast gels (Bio-Rad, Hercules, Ca, USA). The molecular weight of the recombinant proteins was estimated using Invitrogen™ BenchMark™ Pre-stained Protein Ladder (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 16,000Xg for 30 min at 4°C then protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad #5000205). For each sample ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Eluted samples were run for about 1 cm on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (#4569034, Bio-Rad), and the gel was stained with Pierce™ Zinc Reversible Stain Kit (#24582 ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Immunology 2023Quote: ... Protein concentrations of selected cytokines and chemokines were measured in supernatants of macrophages using a BioPlex MAGPIX Multiplex Reader (BIO-RAD, United States) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... The centrifuged samples (10 μL sample volume) were loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA, Cat # 4561086) and run at 140 V ...
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bioRxiv - Biophysics 2023Quote: ... Cleavage of LD650-labelled GPΔTM was verified by in-gel fluorescence imaging of uncleaved and cleaved protein on a 4-20% polyacrylamide using a ChemiDocTM MP imaging system (Bio-Rad, Hercules, CA) followed by Coomassie staining ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... 50 µg of protein was denatured (95°C for 5 minutes) using lysis and Laemmli sample buffer (161-0737, Bio-Rad, with DTT). Samples were separated on a 10 % gradient gel (Criterion TGX Precast Gel ...
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bioRxiv - Neuroscience 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The gels’ stain-free dye was activated by a 5-min UV exposure and protein was transferred to Immun-Blot low-fluorescence PVDF membrane (Bio-Rad #162-0264) using Hoefer TE77X ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in buffer containing 1% NP-40 and proteins were separated using SDS-PAGE (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad), transferred to low fluorescence PVDF membranes (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Both fractions were analyzed on SDS-PAGE and western blot using 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). For immunoblotting of Rubisco ...
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bioRxiv - Physiology 2024Quote: ... Ten μg (final volume 2 μl for all samples) of serum proteins were loaded onto a nitrocellulose membrane (Bio-rad Laboratories, CA, USA). Then ...
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bioRxiv - Cell Biology 2024Quote: Proteins were detected by chemiluminescence following Western blotting using HRP conjugated secondary antibodies of anti-mouse (Bio-Rad # 170–6516; dilution 1:10,000), anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Neuroscience 2024Quote: ... was then added and the samples were boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated by SDS-PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion™ TGX Stain-Free™ Protein Gels (1656001, Bio-Rad) and 1x SDS-page running buffer (192 mM glycine ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled at 95°C for 5 min and resolved in 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed overnight at 4°C with primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-5 µg of the non-denatured protein samples were diluted 1:1 with the native sample buffer (BIO-RAD #161-0738) and loaded into the polymerized gel ...
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bioRxiv - Neuroscience 2024Quote: ... and the protein bands were visualized with an enhanced chemiluminescence (ECL) solution (Clarity Western ECL Substrate, 1705062 Bio-Rad Labs, Des Plaines, IL). Quantitative analysis of the bands was performed with ImageJ software (version 1.49 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were immunoprobed using an HRP-conjugated secondary antibody (dilution 1:3000) and chemiluminescent substrate (Clarity Western ECL substrate, Bio-Rad, Hercules, CA, USA). Blot image acquisition was performed using a Molecular Imager® Gel Doc™ XR+ system (Bio-Rad ...