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Citations for Bio-Rad :
9401 - 9450 of 9625 citations for Recombinant Human EGFR protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Basically 300 ug of IVIG protein were subjected to isoelectric focusing (IPG) across pH gradients of 4-7 (Biorad, LifeSciences, Hercules, Ca, USA) or pH 6-11 (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to nitrocellulose membrane by semi-dry transfer using the Trans-Blot Turbo Transfer System (BioRad, 1.5 A for 7 min). Membranes were blocked in 5% milk in 1x PBS with 0.1% Tween 20 (PBST ...
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bioRxiv - Physiology 2021Quote: ... Protein was run on Criterion TGX Stain-Free Precast Gels and total protein visualized using the ChemiDoc MP Imaging System (Bio-Rad, Watford, UK). Bands were visualized with Bio-Rad Clarity Western ECL (Watford ...
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bioRxiv - Neuroscience 2021Quote: Protein lysates from SH-SY5Y cells were quantified (bicinchoninic acid protein assay, Pierce) and then electrophoresed in 4—12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad) in 1X TGS Tris/Glycine/SDS Buffer (10X TGS Buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels (BioRad, Hercules, CA, Cat#17000435). In addition ...
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bioRxiv - Bioengineering 2021Quote: Protein (50 μg) was separated by SDS□ polyacrylamide gel electrophoresis and transferred onto PVDF (polyvinylidene fluoride) membrane (Bio-Rad Lab Inc., Hercules, CA) by electro blotting ...
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bioRxiv - Neuroscience 2022Quote: ... chemiluminescent imaging of target proteins was performed with Immobilon Forte Western HRP Substrate (EMD Millpore, Billerica, MA) using the ChemiDoc Imaging Systems (BioRad Laboratories, Hercules, CA). Band density of oxytocin receptor was quantified using Image Lab 6.0 (BioRad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 90°C and loaded on a 4–20% (w/v) Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were separated by electrophoresis and blotted onto a membrane with trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs (Bio-Rad) ...
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bioRxiv - Neuroscience 2019Quote: ... or POPC:palmitoyloleoylphosphatidylserine (POPS (85:15) at a 1:200 protein to lipid ratio by dialysis in the presence of Bio-Beads (Bio-Rad, Hercules, CA) into metal free buffer ...
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bioRxiv - Pathology 2019Quote: ... this amount of protein was broken down via a precipitation and purification kit (ReadyPrep™ 2-D Cleanup Kit, Bio-Rad, Warsaw, Poland). The obtained protein pellets were then dissolved in a rehydration buffer ...
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bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
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bioRxiv - Microbiology 2019Quote: ... The values were normalized according to the total protein quantified by Bradford using the Quick Start™ Bradford reagent (BioRad, Hercules, CA, USA). Values were expressed as the relative GFP fluorescence intensity ...
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bioRxiv - Cell Biology 2020Quote: Purified recombinant proteins and protein extracts prepared from harvested cells or immunoprecipitation experiments in reducing Laemmli Sample Buffer were run on 4–20 % Mini-PROTEAN® TGX™ Precast Protein Gels of different comb and well-sizes (Bio-Rad, #456-1093 ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein was measured from the 50 μL sample of homogenate (described above) using a Quick Start Bradford assay (BioRad, Cat. Number 5000205). Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant containing approximately 15-30 μg protein was separated by SDS-PAGE using a 4-20% gradient Mini-PROTEAN TGX Precast Gel (Bio-Rad, Hercules, CA) and then transferred to a 0.2 μM nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on a 10% SDS polyacrylamide gel and visualised with Silver Stain Plus Kit (Bio-Rad Laboratories AB, Sweden) according to manufacturer’s modified silver stain protocol.
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bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
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bioRxiv - Microbiology 2021Quote: ... Proteins were quantified by the Bradford method and 20 to 30 μg of total protein extract were loaded on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). After transfer onto nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2019Quote: ... and equal amounts of total protein (10 to 20 µg) were loaded on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad). All samples for a participant were loaded in adjacent lanes on the same gel ...
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bioRxiv - Synthetic Biology 2019Quote: ... The protein pellet was resuspended in 100 mM ammonium bicarbonate (AMBIC) with 20% methanol and quantified by the Lowry method (Bio-Rad DC assay). A total of 100 µg of protein was reduced by adding tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were normalized for protein concentration using a BCA assay kit (Thermo 23225) and mixed with Laemmli buffer (Bio-Rad 161-0737). 50 µg of the protein mix were run through a 4-20% Criterion TGX gel (Bio-Rad 5671093 ...
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bioRxiv - Microbiology 2020Quote: ... the outer membrane control proteins OmpA (α-OmpA was kindly provided by Prasadaro Nemani (45)) or MOMP (α-MOMP, MONOFLUO™ Legionella pneumophila IFA Test Kit, BioRad), the inner membrane control protein LepB (antibody kindly provided by Gunnar von Heinje (46) ...
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bioRxiv - Neuroscience 2021Quote: Denatured total cellular protein samples were separated on SDS polyacrylamide gels 4-15% Mini-PROTEAN® TGX Stain-Free™ gels (Bio-Rad) and transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... Protein separation was carried out via SDS-PAGE in 12% polyacrylamide gels (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad, Munich, Germany) in TGS buffer (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Consistent protein loading and sufficient transfer of proteins was confirmed using 1× Amido Black stain and images were captured using a Versadoc System (Bio-Rad, Mississauga, ON). Membranes were blocked overnight at 4°C with Tris-buffered saline (TBS)/0.1% Tween 20 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... protein samples were boiled for 5 min in 1x Laemmli sample buffer and separated on an SDS-PAGE using Mini-PROTEAN® TGXTM Precast Protein Gels or 10–20% Mini-PROTEAN® Tris-Tricine Gels (Bio-Rad). Blotting was performed on a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Total proteins in the gel or on the membrane were visualized using Coomassie (InstantBlue, Sigma-Aldrich, scanned with the Odyssey or BioRad Chemidoc imagers) or Ponceau S staining ...
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bioRxiv - Genetics 2019Quote: ... Total protein was quantified using the BCA assay and 25-50μg of protein in 1x Lamelli Buffer with β-mercaptoethanol was loaded onto 4-15% TGX protean gels (Bio-Rad, Hercules, CA). The protein was transferred to a .2μm PVDF membrane by wet transfer ...
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bioRxiv - Synthetic Biology 2021Quote: ... The gel was then immediately placed into a Bio-Rad TransBlot® Turbo apparatus for protein transfer onto a nitrocellulose membrane (Bio-Rad #1704158). Since all samples were produced from the same batch of cell-free expression reaction mix or were of known concentration ...
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bioRxiv - Biophysics 2020Quote: ... the samples were solubilized with standard Laemmli sample buffer and separated by electrophoresis using sodium dodecyl sulfate polyacrylamide gel prepared using Any kD TGX precast protein gels (Bio-Rad, Hercules, California). Separated proteins in gel were electroblotted onto a polyvinylidene difluoride membrane using Trans-Blot Turbo transfer system according to the manufacturer’s instruction (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: Cellular lysates were incubated at 95°C for 5 min and separated in 12% Mini-PROTEAN TGX Precast Protein gels (BIO-RAD, Cat #4561044). Proteins were then transferred to 0.45 μm PVDF membrane at 100V for 1 hr ...
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bioRxiv - Cancer Biology 2021Quote: The protein extracts from the cells (40 μg) were subjected to electrophoresis in SDS-PAGE on 4–15% precast protein gels (Bio-Rad, Hercules, CA), separated and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: 2 μg of total protein derived from meiotic or post-meiotic germ cell populations was loaded onto a Mini-PROTEAN TGX gel (Bio-Rad, 456-8093) and separated by SDS-PAGE for 1 hour at 150V ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were fractionated via 8 ∼ 14% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using a Trans-blot apparatus (Bio-Rad, Hercules, CA). The PVDF membranes were then sequentially subject to blocking ...
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bioRxiv - Cell Biology 2021Quote: ... Forty µg of denaturated proteins were subjected to a Western blot analysis after 4–15 % MiniPROTEAN® TGX StainFree™ gel electrophoresis (Bio-Rad) followed by probing antibodies against TLR4 or β-Actin (TLR4:1:400 ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories, 456-8084). Electrophoresis was performed in 1x Tris/Glycine Buffer (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates (32 μg proteins/lane) were loaded to and separated on 10% SDS-PAGE gels and electrophoretically transferred to PVDF membrane (1620264, Bio-Rad, Hercules, CA). The membranes were blocked in 5% BSA/TBST (0.1% Tween-20 ...
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bioRxiv - Cell Biology 2022Quote: ... 20-30 μg of protein extract was loaded onto 4 to 20% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Bio-Rad, Hercules, CA) and transferred to Immobilon-P PVDF membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were boiled 1 min and subjected to SDS-PAGE using a 4-15% Mini-PROTEAN TGX Stain-Free Protein Gel (Bio-Rad Ref. 4568085). After SDS-PAGE ...
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bioRxiv - Genetics 2022Quote: ... Approximately 20 μg of each sample were loaded on a 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad cat no. 4561094). Proteins were transferred to a Nitrocellulose Membrane (Bio-Rad cat no ...
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bioRxiv - Genetics 2022Quote: ... Duplicate samples run on the same gels were used for total protein staining with QC Colloidal Coomassie Stain (Bio-Rad cat no. 1610803).
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bioRxiv - Microbiology 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... An equal amount of total protein (20 – 50 μ per sample) was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620177) for blotting.
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bioRxiv - Neuroscience 2022Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% Criterion TGX precast Midi protein gel (Bio-Rad, Cat#5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2022Quote: ... steady-state levels of protein were detected by Coomassie-staining of the gel and scanning employing the Gel Doc XR+ System (Bio-Rad, USA, Hercules). Signals were quantified with ImageJ 1.53e (29).
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Physiology 2022Quote: ... The samples were prepared to contain equal concentrations (1 μg·μl-1) of protein in 1x Laemmli buffer (cat#1610747, Bio-Rad, Hercules, California, USA) and stored at −80°C for future analysis.
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bioRxiv - Molecular Biology 2022Quote: ... the protein bands were detected by LumiQ HRP Substrate solution (Share-bio, SSA003) and imaged with ChemiDoc™MP imaging system (Bio-Rad, USA)
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bioRxiv - Cancer Biology 2024Quote: Total protein concentrations were determined against a standard curve of BSA in a 96-well plate using the DC™ Protein assay (Bio-Rad Laboratories). Proteins were separated on reducing 10% NuPAGE™ Novex™ Bis-Tris Protein Gels and transferred onto PVDF membranes ...
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bioRxiv - Bioengineering 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...