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Citations for Bio-Rad :
9401 - 9450 of 9474 citations for Recombinant Human ITGAV & ITGB6 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: 20µg of protein extracts or 10% Co-IP samples were resolved on 10-12% SDS–PAGE and proteins were transferred to PVDF membranes using a semi dry Western blot system (Bio-Rad Trans-Blot SD). Membranes were blocked in 5% non-fat milk in PBS-T (1x PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein were resolved by sodium dodecyl sulfate-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA, USA). Blots were probed with the indicated primary antibodies listed in Extended Data Table 4 ...
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bioRxiv - Genomics 2022Quote: ... XCell II Blot module was used for wet electrophoretic transfer of proteins from SDS-PAGE gel to nitrocellulose membrane (Biorad, Cat # 1620145, 0.45 μm) in Tris-Glycine Electroblotting buffer (National Diagnostic ...
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bioRxiv - Immunology 2022Quote: The purity and quality of the proteins were characterized at all stages of the purification process using 4%-20% SDS–PAGE (Bio-Rad, Catalog number 4561096) and 3%-12% Bis-Tris gel (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μl of serial dilutions of standard protein and samples were mixed with 250 μl of Bradford reagent (500-0205, BIO-RAD Quick Start™).
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bioRxiv - Microbiology 2024Quote: ... purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a 12% polyacrylamide gel using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of proteins (20 μg) were separated by SDS-PAGE and then transferred onto nitrocellulose membranes (ref 1704271, Bio-Rad, Hercules, California, USA). Membranes were incubated in blocking buffer ...
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bioRxiv - Developmental Biology 2024Quote: Protein lysates (40 µg per lane) from uteri or oviducts were loaded on a 4-15% SDS-PAGE gel (Bio-Rad, Hercules, CA, USA), separated in 1X Tris-Glycine Buffer (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–20% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a PVDF membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein concentration of pooled saliva samples was measured to be approximately 20 mg/mL using the Bradford assay kit (Bio-Rad, Hercules, CA, USA) and bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2023Quote: ... the protein concentrations were determined by image analysis of SDS-PAGE with 4-20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad, 4568096) followed by Coomassie Brilliant Blue staining using the following concentration standard ...
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bioRxiv - Molecular Biology 2023Quote: ... After gel migration proteins were transferred to Amersham nitrocellulose Protran Western blotting membranes (GE10600001) using semi-dry transfer (SEMI DRY BLOT APPARATUS, Bio-Rad, cat. no.1703940). Blots were blocked in TBS-T and 5 % milk and probed with relevant antibodies overnight ...
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bioRxiv - Immunology 2023Quote: ... a gradient of 200 ng to 1 ng of protein were resolved by SDS-PAGE using AnykD Mini-Protean TGX gels (Bio-Rad Laboratories, Hercules, CA) and transferred to nitrocellulose membranes (0.45 μm pore size ...
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bioRxiv - Plant Biology 2023Quote: ... Seed storage proteins were visualized from 100 µg of seed material on a 12% SDS-PAGE compared to a Precision Plus Dual Color Protein Standard (Bio-Rad, Hercules, CA, USA). Gels were stained for 1 hr using a 0.1% Coomassie Brilliant Blue ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was transferred to PVDF membrane (Amersham 10600022) using semi-dry transfer apparatus using the ‘mixed MW’ setting (Bio-Rad trans blot turbo). Membranes were blocked for 1 hour in 5% powdered milk in TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... Cellular proteins were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis before being electroblotted onto polyvinylidene fluoride membranes (#1704156, Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with the following primary antibodies for 60 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were stained with coomassie blue R250 and PMMoV-I coat protein was quantified using a densitometer and the quantity one software (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of proteins (20 μg) were separated by SDS-PAGE and then transferred onto nitrocellulose membranes (ref 1704271, Bio-Rad, Hercules, California, USA). Membranes were incubated in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Biochemistry 2023Quote: ... Protein yields for both nitrogenase component fractions were determined using the Quick Start™ Bradford 1x Dye Reagent (Bio-Rad Laboratories, Inc., Hercules, USA) according to the instructions by the manufacturer and purity of both protein fractions was analysed via sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biochemistry 2023Quote: ... Total protein concentrations of the cell extracts were determined using the Bio-Rad Protein Assay Kit according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA) using BSA as standard.
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bioRxiv - Biochemistry 2023Quote: ... and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores) using a Trans Blot Turbo system (Bio-Rad Laboratories S.r.l., Segrate, Italy) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 25ug of protein was used for Western blot run in 4-20% gradient gel (Criterion TGX Stain-free, #5678094, Bio-Rad, Hercules, California, USA). Proteins were transferred to nitrocellulose membrane in Turboblotter (Bio-Rad ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: Cell or tissue lysates (10–30 μg protein) or immunoprecipitated samples were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against leptin (Cat# ...
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bioRxiv - Immunology 2023Quote: ... Western blotting was performed by electrophoresis of 20 µg of proteins on Mini-PROTEAN or Criterion TGX Precast Gels (Bio-Rad, Hercules, CA, USA), followed by electrotransfer to Amersham™ Protran® nitrocellulose membrane (Millipore Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... 10*106 virions of IMP-1088 or DMSO released purified viruses were lysed in 4x Laemmli protein sample buffer (BIO-RAD, Cat. no. 1610747). Samples were electrophoresed on 4-20% precast polyacrylamide gel (BIO-RAD ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein (20 μg/lane) were resolved in 10% Bis-Tris Criterion™ XT Precast Gels and XT MOPS running buffer (BioRad, Cat# 1610799, USA) at 120 volts for 1.5 h using Criterion™ vertical electrophoresis cell (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... 30µg of protein homogenates were separated by SDS-PAGE on a 4-12% gradient Bis-Tris gel (Bio-Rad, Hercules, CA, Criterion XT 3450125) in XT MES buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (10 µg of protein per well) were separated on SDS-polyacrylamide gels (CriterionTM TGXTM Precast Gels, 4-15%; Bio-Rad, Hercules, CA, USA) using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The collected protein samples were electrophoresed on 10% SDS-polyacrylamide gel (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Cat. No. 1620177). After blocking with 5% bovine serum albumin (BSA ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Whole cell lysates containing SDS buffer were homogenized with a 27-G syringe and resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels and transferred to nitrocellulose membranes (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... A 40 µg of protein of CFS samples and 10 ng of purified rGdhA were resolved in a 4-20% SDS-PAGE (Bio-Rad, Hercules, CA; # 4561094) and immunoblotted with affinity purified anti-rabbit rGdhA antibody (0.3 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... at 95°C for 10 mins before being loaded into 4-20% Mini-PROTEAN® TGXTM Precast Protein Gels (15 wells, 15uL; Bio-Rad, CA, USA) and subject to electrophoresis on a Bio-Rad Mini-PROTEAN® Tetra Cell system (180 V ...
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bioRxiv - Cell Biology 2020Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels and the Trans-Blot® Turbo™ transfer system (Bio-Rad). The following antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Precision Plus Protein™ Dual Color Standards and Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit were purchased from Bio-Rad (Hercules, CA). Antibodies against Phospho-c-Raf (Ser338) ...
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bioRxiv - Cell Biology 2020Quote: Equal amounts of protein were loaded onto 12% (v/v) resolving SDS-PAGE gels with 4% (v/v) polyacrylamide stacking gels using Precision Plus Protein™ dual colour standards (Bio-Rad, Hercules, CA, USA). The gels were run in a Mini-Protean® Tetra Cell system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Purified proteins were eluted in SDS-PAGE sample loading buffer and separated in 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad# 4561094, 4561096) either for western blot (WB ...
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bioRxiv - Molecular Biology 2019Quote: ... All reactions were quenched with 6X SDS loading buffer and fractionated on an 8% tris-glycine SDS-PAGE gel along with a protein ladder (BioRad, Precision Plus Dual Color Standard). A photograph of the dried protein gel was overlaid with the scanned phosphorimage of the gel to estimate the molecular weights of bands on the phosphorimage.
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bioRxiv - Biochemistry 2021Quote: ... D3_Sof protein was immobilized covalently on the surface of one of the vertical channels of the GLC sensor chip (Bio-Rad Laboratories, Hercules, CA USA) to a 100 RU level ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was determined using a commercial kit (Pierce™ BCA Protein Assay Kit, Thermo Fischer) and measured at 595 nm with a spectophotometer (BioRad iMarkTM Micro Plate Reader). Each sample was prepared using 30 μg of total proteins combined with the appropriate amount of 5X loading buffer (10% β-Mercaptoethanol ...
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bioRxiv - Biochemistry 2022Quote: LiRecT protein was buffer exchanged into 100 mM ammonium acetate pH 7 (unadjusted) using Micro BioSpin P6 spin columns (Bio-Rad Laboratories, Hercules, CA, USA). All ssDNAs were dialyzed into 100 mM ammonium acetate with Pierce 96-well microdialysis devices with 3.5K MWCO (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of proteins from lysates were separated by SDS-PAGE using Mini-PROTEAN® TGX™ 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... and heated to 95°C for 10 minutes before being loaded into precast gels (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad, Cat. # 4561094, 4561096). The Precision Plus Protein™ Dual Color Standards (BioRad ...
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bioRxiv - Cell Biology 2019Quote: Reduced and heat-denatured protein extracts were separated by SDS-PAGE (4–20% Mini-4– 20% Mini-PROTEAN® TGX™ Gel, Bio-Rad, 456-1094) and then transferred onto 0.2 µm nitrocellulose membranes (Trans-Blot Turbo Transfer Pack ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were transferred to new tubes containing 87% glycerol (final concentration of 10%) and concentrations were adjusted using Bio-Rad protein assay (Bio-Rad, Hercules, California, United States).
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bioRxiv - Plant Biology 2021Quote: ... Protein concentrations were quantified using the Coomassie Blue dye binding method (Bradford, 1976) using a concentrated dye purchased from BioRad (500-0006, Hercules, CA, USA). AsA and DHA concentrations were determined by an ascorbate oxidase based method according to Lin et al ...
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bioRxiv - Neuroscience 2021Quote: ... or 40 μg (splenocytes of WT and Pik3cd−/− mice) of lysate were loaded onto 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories B.V.). Transfer was performed onto Immobilon®-P PVDF Membranes (Merck KGaA) ...
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bioRxiv - Neuroscience 2020Quote: Immunoprecipitation of transfected COS-7 cells or C2C12 myotubes transduced with shVangl2 or shScr was performed as described previously (Strochlic et al., 2012) with SureBeads™ protein G magnetic beads (BioRad, catalog no. 161-4023).