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Citations for Bio-Rad :
9201 - 9250 of 9474 citations for Recombinant Human ITGAV & ITGB6 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins from total and bound fractions were separated by 7.5 % SDS-PAGE and transferred to polyvinylidene difluoride membranes (Bio-Rad, Hercules, CA). To reduce the background signal ...
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bioRxiv - Cell Biology 2024Quote: ... Then an equal amount of proteins were separated on a polyacrylamide gel with pre-cast gradient (Mini-PROTEAN® Precast Gels, acrylamide 4% −20%, Bio-Rad) and transferred to nitrocellulose membrane (Trans-Blot® Turbo ™ Midi Nitrocellulose Transfer Packs ...
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bioRxiv - Plant Biology 2024Quote: ... The protein bands containing PSII supercomplexes were cut out and eluted by a Model 422 Electro-Eluter (Bio-Rad, Hercules, CA, USA). The relative amount of PSII supercomplexes were quantified by dot immunoblot analysis with D1 antibody (Agrisera ...
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bioRxiv - Biochemistry 2024Quote: ... and boiled at 95° C for 5 minutes before separation on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). In-gel fluorescence was imaged first ...
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bioRxiv - Cancer Biology 2024Quote: ... Thirty micrograms of each protein sample were separated by SDS-PAGE and transferred onto a nitrocellulose membrane using the Transblot Turbo Transfer System (Bio-Rad, 1704150EDU). Non-specific binding sites on the membranes were blocked with 5% BSA in TBS-Tween 0.1% for 60 min ...
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bioRxiv - Microbiology 2024Quote: ... 8 μL of lysate were loaded in each lane and resolved on a 7.5% Mini-PROTEAN® TGX™ precast protein gel (Biorad 4561025) for PBP1a and PBP1b samples ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 20 μg of total protein was loaded onto each lane of SDS-PAGE on a 10% acrylamide gel (BIO-RAD cat. #4561034) and run 45 min at 60 V followed by 45 min at 110 V ...
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bioRxiv - Microbiology 2024Quote: ... The blots were washed three times in PBS-T and the proteins were visualized using Clarity Western ECL blotting substrate (Bio-Rad, #1705061) or SuperSignal West Femto (ThermoScientific ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 mins and subjected to SDS-PAGE in a 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad) using XT MES Running Buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein samples were mixed with LDS sample loading buffer at 4X concentration followed by addition of beta-mercaptoethanol (5%) (Bio-Rad, Hercules, CA). The resultant mixture was denatured at 95°C for 9 min ...
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bioRxiv - Cell Biology 2020Quote: ... 20 mg of total protein was separated by SDS-PAGE on polyacrylamide gels (10%) and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Inc.). The membrane was probed with antibodies against Flag (#F7425 ...
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bioRxiv - Cell Biology 2020Quote: ... The purity and integrity of the proteins was evaluated for each batch by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-15% gradient gels (Mini-PROTEAN® TGX® Precast Protein Gels, Bio-Rad) with a Precision Plus Protein® Kaleidoscope® (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty micrograms of protein were loaded on 4-15% gradient TGX Stain-Free™ Protein Gels and transferred to Immun-Blot® LF PVDF membrane (Bio-Rad). The membranes were blocked with 1X TBS with 0.05% Tween 20 and 5% skim milk powder for 2 hours at 25°C ...
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bioRxiv - Neuroscience 2021Quote: ... Around 10-15 μl (around 20 μg of protein) were resolved by SDS-PAGE polyacrylamide gel electrophoresis using 4-20 % precast gradient gels (Bio-Rad, 456-1096), and proteins were electrophoretically transfected onto nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... The blot was washed and incubated with SuperSignal™West Femto (Thermo Fischer Scientific, USA) to reveal the proteins with ChemiDoc™ Touch Imaging System (BioRad, USA).
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bioRxiv - Genomics 2020Quote: ... Nuclei were resuspended in Laemmli buffer and loaded into 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Cat #4561093). SDS-PAGE and protein blotting were performed according to (Dawe et al ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells were transiently transfected in 24-well plates for RNA analysis and in 10 cm plates for protein extractions and IP respectively using a TransFectinTM reagent (Bio-Rad, Hercules, California). 4 h after transfection ...
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bioRxiv - Physiology 2022Quote: ... and protein content assessed by BCA assay (Pierce) before 15 ug protein lysates were separated on a TGX Stain-Free™ criterion gel (Bio-Rad, Sweden). Following which ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μg proteins were loaded and separated by SDS-PAGE gels (10%) electrophoretically and transferred to polyvinylidene difluoride membrane (Bio-Rad Incorporation, 10026933). Blocking was performed by adding 5% BSA for 60 min at room temperature on the shaker ...
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bioRxiv - Molecular Biology 2021Quote: ... The boiled proteins were separated on 4% to 15% SDS-PAGE gradient gels and transferred to LF PVDF membrane (Bio-Rad, Cat #1620260). The membranes were blocked in 5% skim milk in TBST (20 mM-Tris ...
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bioRxiv - Cell Biology 2022Quote: ... the intestine-specific intestinal fatty acid binding protein promoter (-2.3kb fabp2 -also called ifabp-) was amplified by high fidelity PCR (iProof_ High-Fidelity DNA Polymerase; Bio-Rad, Hercules, CA, USA) from p5E–2.3ifabp plasmid (kindly gifted by J ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were centrifuged at 16,000 x g to pellet cellular debris and protein concentration was determined by BCA quantification assay (Bio-Rad, Hercules, CA, USA). For each sample ...
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bioRxiv - Cell Biology 2019Quote: ... For each sample 50 μg of protein were incubated at 95°C for 10 min and then loaded into a gradient (4-15%) western blot gel (mini-protein TGX gel, Bio-Rad, Milan, Italy). Proteins were transferred onto a 0.45 μm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biophysics 2019Quote: ... was performed before staining the gel with Coomassie Blue and imaging overall protein content using a ChemiDoc MP instrument (Bio-Rad, Hercules, CA).
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bioRxiv - Microbiology 2019Quote: ... 60 μg of protein extract for each strain analyzed was loaded into a 10% TGX Stain-Free™ FastCast™ Acrylamide gel (Bio-Rad). Separated proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ transfer system (Bio-Rad) ...
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bioRxiv - Biophysics 2019Quote: ... The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad, Hercules, California). In all purification steps ...
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bioRxiv - Plant Biology 2019Quote: Nectars and the protein extracts were separated by stain-free 1D SDS-PAGE (Bio-Rad’s Mini PROTEAN® TGX Stain-Free™ Gels). For western blot assays ...
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bioRxiv - Cancer Biology 2019Quote: ... CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories, Inc.) and imaged on ChemiDoc MP (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2019Quote: ... Gels were run at 150 V for 1 hour and the protein were subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad, Hercules, CA, USA). All membranes were stained with Ponceau as a qualitative check for equivalent of total protein loading and transfer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein separation was done by 1D SDS-PAGE electrophoresis using commercial gels to ensure reproducibility (8–16% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad). A sample of venom of ISm and ISy lines ...
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bioRxiv - Cancer Biology 2020Quote: Proteins from WCL samples were separated on 4-20% (w/v) Tris-Glycine SDS-PAGE gels (Bio-Rad; Cat#456-1094, Hercules, CA). We loaded 30-50 μg protein per sample ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µg of each protein sample was separated in one dimension using 4-20% tris-glycine gels (Mini-PROTEAN TGX, Bio-Rad Laboratories, Inc.). A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are listed in Supplemental table 2 Immunoreactive proteins were visualized with Clarity Western ECL Substrate (Bio-Rad Laboratories, Hercules, CA, USA). The UVP ChemStudio (Analytik Jena US LLC ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred from the gel onto a 0.2 µm nitrocellulose membrane using the wet transfer method (Bio-Rad Laboratories, Hercules, CA). Overnight transfer was performed for 16 h using a constant voltage of 30V ...
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bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE (90 min at 150V) using 4-20% Criterion™ TGX Stain-Free™ gel (Bio-Rad, 5678095) and electro-blotted onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were separated by SDS-PAGE and blotted onto a PVDF membrane as recommended by the manufacturer (Transblot, Bio-Rad Laboratories, USA). The membrane was blocked overnight at 4 °C in a solution of 5 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... and phospho-Akt (p-Akt) were quantified in 10 μg of total protein using the Bio-Plex Pro magnetic phosphoprotein detection assay (Bio-Rad, United Kingdom). Phosphoprotein expression was normalized to β-actin and expressed as fold-change relative to the uninfected (UI ...
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bioRxiv - Microbiology 2021Quote: ... 20 µL sample was loaded per each lane of an any KD Mini-PROTEAN® TGX™ Precast Protein Gel (BioRad Hercules, CA) and run at 150 V for 1 hour ...
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bioRxiv - Bioengineering 2019Quote: ... Proteins (15 μg each) were separated based on their molecular weight with SDS–PAGE on 10% Mini-PROTEAN gels (Bio-Rad, Hercules, CA) and then transferred to PVDF membranes (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Equal total protein amounts for each sample were used in co-immunoprecipitation assays and analyzed by SDS-PAGE on Criterion gels (Bio-Rad, Hercules, CA). Co-immunoprecipitation assays were performed as described (49 ...
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bioRxiv - Systems Biology 2022Quote: ... sample buffer (5 minutes at 95°C) and subjected to gel electrophoresis (4– 15% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and immunoblotting (polyvinylidene difluoride Transfer Membrane ...
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bioRxiv - Neuroscience 2022Quote: ... The nascent and total protein samples were then run on a 4-12% gradient SDS-polyacrylamide gel along with Precision Plus Protein Kaleidoscope ladder (Bio-Rad, Cat #1610395). Then the proteins were transferred onto nitrocellulose membranes using either a Bio-Rad Trans-Blot Turbo Transfer system (25V ...
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bioRxiv - Biophysics 2022Quote: The purified proteins of V2HeR3 were reconstituted into a mixture of POPE and POPG membranes (molar ratio = 3:1) with a protein-to-lipid molar ratio of 1:20 by removing DDM using Bio-Beads (SM-2; Bio-Rad, CA, USA). The reconstituted samples were washed three times with 1 mM NaCl and 2 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Immunology 2021Quote: ... Basically 300 ug of IVIG protein were subjected to isoelectric focusing (IPG) across pH gradients of 4-7 (Biorad, LifeSciences, Hercules, Ca, USA) or pH 6-11 (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to nitrocellulose membrane by semi-dry transfer using the Trans-Blot Turbo Transfer System (BioRad, 1.5 A for 7 min). Membranes were blocked in 5% milk in 1x PBS with 0.1% Tween 20 (PBST ...
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bioRxiv - Physiology 2021Quote: ... Protein was run on Criterion TGX Stain-Free Precast Gels and total protein visualized using the ChemiDoc MP Imaging System (Bio-Rad, Watford, UK). Bands were visualized with Bio-Rad Clarity Western ECL (Watford ...