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Citations for Bio-Rad :
9351 - 9400 of 9412 citations for Recombinant Human PDCD1LG2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... a gradient of 200 ng to 1 ng of protein were resolved by SDS-PAGE using AnykD Mini-Protean TGX gels (Bio-Rad Laboratories, Hercules, CA) and transferred to nitrocellulose membranes (0.45 μm pore size ...
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bioRxiv - Plant Biology 2023Quote: ... Seed storage proteins were visualized from 100 µg of seed material on a 12% SDS-PAGE compared to a Precision Plus Dual Color Protein Standard (Bio-Rad, Hercules, CA, USA). Gels were stained for 1 hr using a 0.1% Coomassie Brilliant Blue ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was transferred to PVDF membrane (Amersham 10600022) using semi-dry transfer apparatus using the ‘mixed MW’ setting (Bio-Rad trans blot turbo). Membranes were blocked for 1 hour in 5% powdered milk in TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... Cellular proteins were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis before being electroblotted onto polyvinylidene fluoride membranes (#1704156, Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with the following primary antibodies for 60 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were stained with coomassie blue R250 and PMMoV-I coat protein was quantified using a densitometer and the quantity one software (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of proteins (20 μg) were separated by SDS-PAGE and then transferred onto nitrocellulose membranes (ref 1704271, Bio-Rad, Hercules, California, USA). Membranes were incubated in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Biochemistry 2023Quote: ... Protein yields for both nitrogenase component fractions were determined using the Quick Start™ Bradford 1x Dye Reagent (Bio-Rad Laboratories, Inc., Hercules, USA) according to the instructions by the manufacturer and purity of both protein fractions was analysed via sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biochemistry 2023Quote: ... Total protein concentrations of the cell extracts were determined using the Bio-Rad Protein Assay Kit according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA) using BSA as standard.
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bioRxiv - Biochemistry 2023Quote: ... and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores) using a Trans Blot Turbo system (Bio-Rad Laboratories S.r.l., Segrate, Italy) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 25ug of protein was used for Western blot run in 4-20% gradient gel (Criterion TGX Stain-free, #5678094, Bio-Rad, Hercules, California, USA). Proteins were transferred to nitrocellulose membrane in Turboblotter (Bio-Rad ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: Cell or tissue lysates (10–30 μg protein) or immunoprecipitated samples were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against leptin (Cat# ...
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bioRxiv - Immunology 2023Quote: ... Western blotting was performed by electrophoresis of 20 µg of proteins on Mini-PROTEAN or Criterion TGX Precast Gels (Bio-Rad, Hercules, CA, USA), followed by electrotransfer to Amersham™ Protran® nitrocellulose membrane (Millipore Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... 10*106 virions of IMP-1088 or DMSO released purified viruses were lysed in 4x Laemmli protein sample buffer (BIO-RAD, Cat. no. 1610747). Samples were electrophoresed on 4-20% precast polyacrylamide gel (BIO-RAD ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein (20 μg/lane) were resolved in 10% Bis-Tris Criterion™ XT Precast Gels and XT MOPS running buffer (BioRad, Cat# 1610799, USA) at 120 volts for 1.5 h using Criterion™ vertical electrophoresis cell (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... 30µg of protein homogenates were separated by SDS-PAGE on a 4-12% gradient Bis-Tris gel (Bio-Rad, Hercules, CA, Criterion XT 3450125) in XT MES buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (10 µg of protein per well) were separated on SDS-polyacrylamide gels (CriterionTM TGXTM Precast Gels, 4-15%; Bio-Rad, Hercules, CA, USA) using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The collected protein samples were electrophoresed on 10% SDS-polyacrylamide gel (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Cat. No. 1620177). After blocking with 5% bovine serum albumin (BSA ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Whole cell lysates containing SDS buffer were homogenized with a 27-G syringe and resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels and transferred to nitrocellulose membranes (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... A 40 µg of protein of CFS samples and 10 ng of purified rGdhA were resolved in a 4-20% SDS-PAGE (Bio-Rad, Hercules, CA; # 4561094) and immunoblotted with affinity purified anti-rabbit rGdhA antibody (0.3 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... at 95°C for 10 mins before being loaded into 4-20% Mini-PROTEAN® TGXTM Precast Protein Gels (15 wells, 15uL; Bio-Rad, CA, USA) and subject to electrophoresis on a Bio-Rad Mini-PROTEAN® Tetra Cell system (180 V ...
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bioRxiv - Cell Biology 2020Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels and the Trans-Blot® Turbo™ transfer system (Bio-Rad). The following antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Precision Plus Protein™ Dual Color Standards and Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit were purchased from Bio-Rad (Hercules, CA). Antibodies against Phospho-c-Raf (Ser338) ...
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bioRxiv - Cell Biology 2020Quote: Equal amounts of protein were loaded onto 12% (v/v) resolving SDS-PAGE gels with 4% (v/v) polyacrylamide stacking gels using Precision Plus Protein™ dual colour standards (Bio-Rad, Hercules, CA, USA). The gels were run in a Mini-Protean® Tetra Cell system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Purified proteins were eluted in SDS-PAGE sample loading buffer and separated in 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad# 4561094, 4561096) either for western blot (WB ...
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bioRxiv - Molecular Biology 2019Quote: ... All reactions were quenched with 6X SDS loading buffer and fractionated on an 8% tris-glycine SDS-PAGE gel along with a protein ladder (BioRad, Precision Plus Dual Color Standard). A photograph of the dried protein gel was overlaid with the scanned phosphorimage of the gel to estimate the molecular weights of bands on the phosphorimage.
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bioRxiv - Biochemistry 2021Quote: ... D3_Sof protein was immobilized covalently on the surface of one of the vertical channels of the GLC sensor chip (Bio-Rad Laboratories, Hercules, CA USA) to a 100 RU level ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was determined using a commercial kit (Pierce™ BCA Protein Assay Kit, Thermo Fischer) and measured at 595 nm with a spectophotometer (BioRad iMarkTM Micro Plate Reader). Each sample was prepared using 30 μg of total proteins combined with the appropriate amount of 5X loading buffer (10% β-Mercaptoethanol ...
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bioRxiv - Biochemistry 2022Quote: LiRecT protein was buffer exchanged into 100 mM ammonium acetate pH 7 (unadjusted) using Micro BioSpin P6 spin columns (Bio-Rad Laboratories, Hercules, CA, USA). All ssDNAs were dialyzed into 100 mM ammonium acetate with Pierce 96-well microdialysis devices with 3.5K MWCO (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of proteins from lysates were separated by SDS-PAGE using Mini-PROTEAN® TGX™ 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... and heated to 95°C for 10 minutes before being loaded into precast gels (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad, Cat. # 4561094, 4561096). The Precision Plus Protein™ Dual Color Standards (BioRad ...
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bioRxiv - Cell Biology 2019Quote: Reduced and heat-denatured protein extracts were separated by SDS-PAGE (4–20% Mini-4– 20% Mini-PROTEAN® TGX™ Gel, Bio-Rad, 456-1094) and then transferred onto 0.2 µm nitrocellulose membranes (Trans-Blot Turbo Transfer Pack ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were transferred to new tubes containing 87% glycerol (final concentration of 10%) and concentrations were adjusted using Bio-Rad protein assay (Bio-Rad, Hercules, California, United States).
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bioRxiv - Plant Biology 2021Quote: ... Protein concentrations were quantified using the Coomassie Blue dye binding method (Bradford, 1976) using a concentrated dye purchased from BioRad (500-0006, Hercules, CA, USA). AsA and DHA concentrations were determined by an ascorbate oxidase based method according to Lin et al ...
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bioRxiv - Neuroscience 2021Quote: ... or 40 μg (splenocytes of WT and Pik3cd−/− mice) of lysate were loaded onto 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories B.V.). Transfer was performed onto Immobilon®-P PVDF Membranes (Merck KGaA) ...
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bioRxiv - Neuroscience 2020Quote: Immunoprecipitation of transfected COS-7 cells or C2C12 myotubes transduced with shVangl2 or shScr was performed as described previously (Strochlic et al., 2012) with SureBeads™ protein G magnetic beads (BioRad, catalog no. 161-4023).
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bioRxiv - Microbiology 2021Quote: ... The free RNA and RNA-protein complexes in gels were transferred onto a nylon membrane and cross-linked using GS Gene Linker™ UV Chamber (Bio-Rad Laboratories, Hercules, CA). The nylon membrane was incubated with 20 μg/mL of proteinase K (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... and protein standards were mixed with Laemmli buffer and loaded on 4-15% Criterion TGX Stain-Free protein gels in 1×□TGS running buffer (all from Bio-Rad, Marnes-la-Coquette, France) and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... The samples were heated with Laemmli sample buffer with 2-mercaptoethanol at 95°C for 20 min and separated on SDS-PAGE using Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Equal amounts of proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes by using Trans-Blot Turbo™ Transfer Pack (Bio-Rad, Hercules, CA, USA). Western blot analysis was performed using primary antibodies against p-ERK (Cell Signaling Technologies Danvers ...
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bioRxiv - Neuroscience 2022Quote: For each lane, ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of clarified lysate (3 x 106 parasite equivalents) were resolved by SDS-PAGE (Mini-PROTEAN®TGX protein gel; Bio-Rad, Hercules, California, USA), transferred to PVDF membrane (Immobilon®-FL ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibodies were detected via chemiluminescence using Westar Supernova (XLS3, 0020, Cyanogen) and the protein bands were visualized in a ChemiDoc XRS + imager (Bio-rad with image Lab software) (Ayagama et al. ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were transferred to a nitrocellulose membrane for 55 min at 15V using a semi-dry electroblotting transfer unit (Bio-Rad, Hercules, CA, United States). Membranes were incubated for 1 hour with blocking buffer (1% dry skim milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to the membranes by semi-dry Western blotting in a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories Inc., CA, USA). Next ...
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bioRxiv - Neuroscience 2024Quote: ... was boiled for 10 min at 97°C and loaded into 8% SDS-PAGE gels with Precision Plus Protein Standard (Bio-Rad, 161-0374/161-0375). Separated proteins were transferred to a nitrocellulose membrane (0.2 μm ...
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bioRxiv - Pathology 2019Quote: ... Samples were mixed with SDS-loading buffer without heating and electrophoretically separated on a 4–20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-rad, cat. number 5671094) and transferred to nitrocellulose membrane ...
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bioRxiv - Neuroscience 2020Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min before loading into a 4 – 20% Criterion TGX precast Midi protein gel (Bio-Rad, Hercules, CA, USA; Cat# 5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...