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Citations for Bio-Rad :
9201 - 9250 of 9412 citations for Recombinant Human PDCD1LG2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
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bioRxiv - Microbiology 2021Quote: ... Proteins were quantified by the Bradford method and 20 to 30 μg of total protein extract were loaded on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). After transfer onto nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2019Quote: ... and equal amounts of total protein (10 to 20 µg) were loaded on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad). All samples for a participant were loaded in adjacent lanes on the same gel ...
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bioRxiv - Synthetic Biology 2019Quote: ... The protein pellet was resuspended in 100 mM ammonium bicarbonate (AMBIC) with 20% methanol and quantified by the Lowry method (Bio-Rad DC assay). A total of 100 µg of protein was reduced by adding tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were normalized for protein concentration using a BCA assay kit (Thermo 23225) and mixed with Laemmli buffer (Bio-Rad 161-0737). 50 µg of the protein mix were run through a 4-20% Criterion TGX gel (Bio-Rad 5671093 ...
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bioRxiv - Microbiology 2020Quote: ... the outer membrane control proteins OmpA (α-OmpA was kindly provided by Prasadaro Nemani (45)) or MOMP (α-MOMP, MONOFLUO™ Legionella pneumophila IFA Test Kit, BioRad), the inner membrane control protein LepB (antibody kindly provided by Gunnar von Heinje (46) ...
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bioRxiv - Neuroscience 2021Quote: Denatured total cellular protein samples were separated on SDS polyacrylamide gels 4-15% Mini-PROTEAN® TGX Stain-Free™ gels (Bio-Rad) and transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... Protein separation was carried out via SDS-PAGE in 12% polyacrylamide gels (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad, Munich, Germany) in TGS buffer (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Consistent protein loading and sufficient transfer of proteins was confirmed using 1× Amido Black stain and images were captured using a Versadoc System (Bio-Rad, Mississauga, ON). Membranes were blocked overnight at 4°C with Tris-buffered saline (TBS)/0.1% Tween 20 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... protein samples were boiled for 5 min in 1x Laemmli sample buffer and separated on an SDS-PAGE using Mini-PROTEAN® TGXTM Precast Protein Gels or 10–20% Mini-PROTEAN® Tris-Tricine Gels (Bio-Rad). Blotting was performed on a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Total proteins in the gel or on the membrane were visualized using Coomassie (InstantBlue, Sigma-Aldrich, scanned with the Odyssey or BioRad Chemidoc imagers) or Ponceau S staining ...
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bioRxiv - Genetics 2019Quote: ... Total protein was quantified using the BCA assay and 25-50μg of protein in 1x Lamelli Buffer with β-mercaptoethanol was loaded onto 4-15% TGX protean gels (Bio-Rad, Hercules, CA). The protein was transferred to a .2μm PVDF membrane by wet transfer ...
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bioRxiv - Synthetic Biology 2021Quote: ... The gel was then immediately placed into a Bio-Rad TransBlot® Turbo apparatus for protein transfer onto a nitrocellulose membrane (Bio-Rad #1704158). Since all samples were produced from the same batch of cell-free expression reaction mix or were of known concentration ...
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bioRxiv - Biophysics 2020Quote: ... the samples were solubilized with standard Laemmli sample buffer and separated by electrophoresis using sodium dodecyl sulfate polyacrylamide gel prepared using Any kD TGX precast protein gels (Bio-Rad, Hercules, California). Separated proteins in gel were electroblotted onto a polyvinylidene difluoride membrane using Trans-Blot Turbo transfer system according to the manufacturer’s instruction (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: Cellular lysates were incubated at 95°C for 5 min and separated in 12% Mini-PROTEAN TGX Precast Protein gels (BIO-RAD, Cat #4561044). Proteins were then transferred to 0.45 μm PVDF membrane at 100V for 1 hr ...
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bioRxiv - Cancer Biology 2021Quote: The protein extracts from the cells (40 μg) were subjected to electrophoresis in SDS-PAGE on 4–15% precast protein gels (Bio-Rad, Hercules, CA), separated and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: 2 μg of total protein derived from meiotic or post-meiotic germ cell populations was loaded onto a Mini-PROTEAN TGX gel (Bio-Rad, 456-8093) and separated by SDS-PAGE for 1 hour at 150V ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were fractionated via 8 ∼ 14% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using a Trans-blot apparatus (Bio-Rad, Hercules, CA). The PVDF membranes were then sequentially subject to blocking ...
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bioRxiv - Cell Biology 2021Quote: ... Forty µg of denaturated proteins were subjected to a Western blot analysis after 4–15 % MiniPROTEAN® TGX StainFree™ gel electrophoresis (Bio-Rad) followed by probing antibodies against TLR4 or β-Actin (TLR4:1:400 ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories, 456-8084). Electrophoresis was performed in 1x Tris/Glycine Buffer (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates (32 μg proteins/lane) were loaded to and separated on 10% SDS-PAGE gels and electrophoretically transferred to PVDF membrane (1620264, Bio-Rad, Hercules, CA). The membranes were blocked in 5% BSA/TBST (0.1% Tween-20 ...
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bioRxiv - Cell Biology 2022Quote: ... 20-30 μg of protein extract was loaded onto 4 to 20% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Bio-Rad, Hercules, CA) and transferred to Immobilon-P PVDF membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were boiled 1 min and subjected to SDS-PAGE using a 4-15% Mini-PROTEAN TGX Stain-Free Protein Gel (Bio-Rad Ref. 4568085). After SDS-PAGE ...
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bioRxiv - Genetics 2022Quote: ... Approximately 20 μg of each sample were loaded on a 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad cat no. 4561094). Proteins were transferred to a Nitrocellulose Membrane (Bio-Rad cat no ...
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bioRxiv - Genetics 2022Quote: ... Duplicate samples run on the same gels were used for total protein staining with QC Colloidal Coomassie Stain (Bio-Rad cat no. 1610803).
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bioRxiv - Microbiology 2022Quote: ... and mixed 1:1 with 2X Laemmli buffer followed by SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels and staining with Coomassie Brilliant Blue R-250 (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... An equal amount of total protein (20 – 50 μ per sample) was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620177) for blotting.
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bioRxiv - Neuroscience 2022Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% Criterion TGX precast Midi protein gel (Bio-Rad, Cat#5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2022Quote: ... steady-state levels of protein were detected by Coomassie-staining of the gel and scanning employing the Gel Doc XR+ System (Bio-Rad, USA, Hercules). Signals were quantified with ImageJ 1.53e (29).
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Physiology 2022Quote: ... The samples were prepared to contain equal concentrations (1 μg·μl-1) of protein in 1x Laemmli buffer (cat#1610747, Bio-Rad, Hercules, California, USA) and stored at −80°C for future analysis.
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bioRxiv - Molecular Biology 2022Quote: ... the protein bands were detected by LumiQ HRP Substrate solution (Share-bio, SSA003) and imaged with ChemiDoc™MP imaging system (Bio-Rad, USA)
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bioRxiv - Cancer Biology 2024Quote: Total protein concentrations were determined against a standard curve of BSA in a 96-well plate using the DC™ Protein assay (Bio-Rad Laboratories). Proteins were separated on reducing 10% NuPAGE™ Novex™ Bis-Tris Protein Gels and transferred onto PVDF membranes ...
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bioRxiv - Bioengineering 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-20 µl of the cell lysates were separated on 4-15% Mini-PROTEAN® TGX™ Stain-FreeTM Protein Gels from Bio-Rad and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates from each cell line were analysed on multiple gels (four per cell line) with Precision Plus Protein Dual Color Standard (1610394, Bio-Rad, Hercules, CA) as ladder and blotted to membranes to separately probe for phosphorylated and total forms of the same proteins ...
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bioRxiv - Systems Biology 2024Quote: 25 μg of RIPA or 5ug of lipid raft protein lysate was loaded onto a dot blot apparatus (Bio-Rad, Hercules, CA, USA) and were filtered through 0.45μm PVDF for 2 hours by gravity filtration ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting pellets were resuspended in resuspension buffer (RB; Table S7) and proteins levels were quantified by Lowry (Bio-Rad #5000113, #5000114, #5000114). 600 µg aliquots of each sample were pre-heated at 95°C for 5 min ...
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bioRxiv - Pathology 2024Quote: ... 15 µg of proteins were resolved on Criterion TGX Stain-Free 4-15%,18 and 26-well gel (Bio-Rad Laboratories, #5678084, #5678085) in a Criterion large format electrophoresis cell (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2024Quote: ... Then we separated proteins into 4-15% gradient SDS-PAGE gels and transferred them to PVDF immuno-blot membranes (Bio-Rad, Hercules, CA). The primary antibodies S6 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The relative amount of transferred protein per sample was determined after Ponceau S staining by a GS-800 densitometer (Bio-Rad, Hercules, USA) and used as loading control ...
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bioRxiv - Cell Biology 2023Quote: ... Total nascent peptides were detected by in-gel imaging at 647 µm and total protein was detected by far red epi imaging after staining with 0.1% Coomassie brilliant blue (BioRad ChemiDoc MP Imaging System). For detection of nascent insulin and tubulin ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times with TBS-Tween 0.2 % for 15 min and immunoreactive proteins were detected with enhanced chemiluminescence reagents according to the manufacturer instructions (Bio-Rad, Hercules, CA USA). Signals were captured with the ChemiDoc system (Bio-Rad ...
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bioRxiv - Pathology 2023Quote: ... The total protein concentrations of each liver samples were determine using a standard curve generated with BSA at different known concentration using the Quick Start™ Bradford Protein Assay (Bio-Rad, U.S.A.). On the basis of the measure protein cencetrions ...
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bioRxiv - Cell Biology 2023Quote: ... then centrifuged at 13,000 rpm for 30 min at 4 °C and the resulting supernatants were subjected to Bradford protein assay (Bio-Rad, Hercules, California, USA) for measuring total protein concentration ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µL of protein lysate was then loaded into 4–20% Criterion™ TGX Stain-Free™ precast gels (Bio-Rad, Cat. #5678094). Proteins were separated at 200V for 45 min and then transferred to a low-fluorescence PVDF membrane at 25V for 30 min using the Trans-Blot Turbo Transfer System and Kit (Bio-Rad ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... ECL signals and total protein loading amounts were measured with a Bio-Rad ChemiDoc MP imaging system (Bio-Rad Laboratories Inc., Hercules, CA). The acquired images were analyzed with Bio-Rad ImageLab software version 6.1 (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... Electrophoresis and blotting of protein extracts were performed using mini-PROTEAN tetra handcast and trans-blot turbo transfer systems (Bio-Rad, Hercules, CA).