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Citations for Bio-Rad :
9051 - 9100 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Protein contents were visualized on a ChemiDoc MP system using the ImageLab software for quantification (both from Bio-Rad Laboratories GmbH, Munich, Germany). For the demonstration of equal loading ...
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bioRxiv - Immunology 2023Quote: ... Same protein amounts were loaded onto 4-12% NuPAGE Gels and protein was transferred to PVDF membranes by using Trans-Blot® Turbo™ Transfer System (Biorad#17001917). Membrane was blocked with 5% milk-TBS ...
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bioRxiv - Physiology 2023Quote: ... 25 µg of total protein was loaded into precast 10 % BIO-RAD Mini-PROTEAN® TGX Stain-FreeTM SDS-polyacrylamide gel (Bio-RAD Laboratories), then electrophoretically transferred to 0.2 µm nitrocellulose membrane (Trans-Blot® TurboTM Transfer Pack ...
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bioRxiv - Molecular Biology 2023Quote: 20-25μg of protein lysate with 4x Laemmli buffer (cat# 1610747; Bio-Rad Laboratories was separated on 4-15% Mini-PROTEAN TGX Precast Protein Gels (cat# 4561086; Bio-Rad Laboratories) and transferred to Immun-Blot PVDF Membranes for Protein Blotting (cat# 1620177 ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein transferred to 0.45 mm polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo transfer system (BioRad Laboratories, Hatfield, PA, USA).
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were first analysed using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) using TGX Stain-Free precast gels (Bio-Rad, Hercules, Ca, USA). The molecular weight of the recombinant proteins was estimated using Invitrogen™ BenchMark™ Pre-stained Protein Ladder (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 16,000Xg for 30 min at 4°C then protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad #5000205). For each sample ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Eluted samples were run for about 1 cm on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (#4569034, Bio-Rad), and the gel was stained with Pierce™ Zinc Reversible Stain Kit (#24582 ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Cell Biology 2023Quote: ... ECL signals and total protein loading amounts were measured with a Bio-Rad ChemiDoc MP imaging system (Bio-Rad Laboratories Inc., Hercules, CA). The acquired images were analyzed with Bio-Rad ImageLab software version 6.1 (Bio-Rad Laboratories).
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bioRxiv - Synthetic Biology 2023Quote: ... The centrifuged samples (10 μL sample volume) were loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA, Cat # 4561086) and run at 140 V ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the concentrated AmGSTD1 protein was injected onto a Hydroxyapatite column (HA) connected to the NGC chromatography System (Bio-Rad Laboratories, Hercules, CA, USA). A gradient of 5 mM NaPi and 5 mM HEPES ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Electrophoresis and blotting of protein extracts were performed using mini-PROTEAN tetra handcast and trans-blot turbo transfer systems (Bio-Rad, Hercules, CA).
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bioRxiv - Systems Biology 2023Quote: Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... Cleavage of LD650-labelled GPΔTM was verified by in-gel fluorescence imaging of uncleaved and cleaved protein on a 4-20% polyacrylamide using a ChemiDocTM MP imaging system (Bio-Rad, Hercules, CA) followed by Coomassie staining ...
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bioRxiv - Cell Biology 2023Quote: ... Total nascent peptides were detected by in-gel imaging at 647 µm and total protein was detected by far red epi imaging after staining with 0.1% Coomassie brilliant blue (BioRad ChemiDoc MP Imaging System). For detection of nascent insulin and tubulin ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μg protein were separated by SDS-PAGE on 4-15% Mini- PROTEAN® TGX Stain-Free™ Gels (Bio-Rad®). and transferred onto PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad®) ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... 50 µg of protein was denatured (95°C for 5 minutes) using lysis and Laemmli sample buffer (161-0737, Bio-Rad, with DTT). Samples were separated on a 10 % gradient gel (Criterion TGX Precast Gel ...
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bioRxiv - Neuroscience 2024Quote: ... was then added and the samples were boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: Proteins were detected by chemiluminescence following Western blotting using HRP conjugated secondary antibodies of anti-mouse (Bio-Rad # 170–6516; dilution 1:10,000), anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-5 µg of the non-denatured protein samples were diluted 1:1 with the native sample buffer (BIO-RAD #161-0738) and loaded into the polymerized gel ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated by SDS-PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion™ TGX Stain-Free™ Protein Gels (1656001, Bio-Rad) and 1x SDS-page running buffer (192 mM glycine ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled at 95°C for 5 min and resolved in 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed overnight at 4°C with primary antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... Both fractions were analyzed on SDS-PAGE and western blot using 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). For immunoblotting of Rubisco ...
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bioRxiv - Physiology 2024Quote: ... Ten μg (final volume 2 μl for all samples) of serum proteins were loaded onto a nitrocellulose membrane (Bio-rad Laboratories, CA, USA). Then ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant protein was transferred to 0.45 μm nitrocellulose membranes using the Trans-Blot® Semi-Dry Transfer Cell (Bio-Rad, CA, USA), and bands were confirmed by 0.1% Amido black staining solution (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and the protein bands were visualized with an enhanced chemiluminescence (ECL) solution (Clarity Western ECL Substrate, 1705062 Bio-Rad Labs, Des Plaines, IL). Quantitative analysis of the bands was performed with ImageJ software (version 1.49 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were immunoprobed using an HRP-conjugated secondary antibody (dilution 1:3000) and chemiluminescent substrate (Clarity Western ECL substrate, Bio-Rad, Hercules, CA, USA). Blot image acquisition was performed using a Molecular Imager® Gel Doc™ XR+ system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... 5μg of either whole brain lysate or tubulin-enriched protein fraction was loaded per lane and run on 4-20% Mini-Protean TGX Stain-Free precast gels (4568093; Bio-Rad Laboratories, Hercules, CA) at 150mV for 1hr ...
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bioRxiv - Molecular Biology 2020Quote: ... Reducing SDS sample buffer was added and proteins resolved on 4%-15% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad, California, United States) or 3%-8% NuPAGE Tris-Acetate Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 µg of WCE were separated on mini protein TGX stain-free gel and electroblotted using the PVDF transfer membrane (Bio-Rad, Hercules, CA, USA). The ChemiDoc™ MP imaging system (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of the resulting solution was then applied to either 4-12% Criterion™ XT Bis-Tris Protein Gels (BioRad, Hercules, CA, USA) or NuPAGE™ 4 – 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... The protein levels were quantified with ImageLab software after acquisition of chemiluminescent signals with a Chemidoc digital imager (Bio-Rad, Marnes-la-Coquette, France). For all SDS-PAGE analyses ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear extracts containing the total of 40 µg protein were separated on a 10% Tris-SDS PAGE gel (Bio-Rad Mini-PROTEAN® TGX). Gels were transferred on nitrocellulose membranes using Bio-Rad TransBlot system ...
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bioRxiv - Physiology 2019Quote: ... The remaining supernatant was diluted to 1.5 µg × µl−1^ total protein in lysis buffer and 4X Laemmli sample buffer (Bio-Rad Laboratories AB, Oslo Norway) containing 2-Mercaptoethanol ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The triglyceride content was normalized to the total protein content of the flies estimated by Quick Start™ Bradford 1X Dye Reagent (BioRad, Cat. # 500-0205). Freshly emerged flies feed less and possess larval triglyceride energy storage ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 μg proteins of the cell line were exposed to a 10% SDS-PAGE gel and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA ...
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bioRxiv - Physiology 2020Quote: ... and equal amounts of total protein (15 or 20 μg) were loaded in different wells on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad, Australia). A stain-free system (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... and purity assessed by 15% SDS-PAGE using Bio Rad Precision Plus Protein All Blue Standards (10-250 kDa) as molecular weight ladder (Bio-Rad; Hercules, CA, USA).
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bioRxiv - Neuroscience 2021Quote: ... Target proteins were detected by chemiluminescence with Clarity™ (Beta-actin and GFP) and Clarity Max™ (SP) Western ECL Substrate kit (Bio-Rad®) on the Odyssey FC system (LI-COR) ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine levels within the lung homogenates of infected mice were analyzed using the Bio-Plex protein array system (Luminex-based technology, Bio-Rad Laboratories, Hercules, CA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...
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bioRxiv - Plant Biology 2020Quote: ... the recombinant protein in the filtrate was further purified by size exclusion chromatography (NGC™ chromatography system with Enrich™ SEC 650 column, BioRad, Munich, Germany) in 50 mM NaH2PO4 /Na2HPO4 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins extracts were boiled at 95°C and then separated onto Mini-PROTEAN ® TGX™ precast protein SDS-PAGE (4-15% gradient) gels (BIO-RAD, #4561083). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... The secondary antibodies were detected via chemiluminescence using Westar Supernova (XLS3,0020, Cyanogen) and the protein bands were visualised in a ChemiDoc XRS+ imager (Bio-rad with image Lab software).
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bioRxiv - Cancer Biology 2021Quote: Westerns were performed by loading 30-50ug of total protein per well into 15 well polyacrylamide gels (Bio-Rad, California, USA, Cat #456-1086) and run for 30min at 200V ...
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bioRxiv - Cancer Biology 2021Quote: Separation of 20 μg or 50 μg protein from cell lysate was performed on a 10% Mini-PROTEAN® TGX™ gel (BioRad, Portland, ME) and subsequently transferred onto Immunoblot PVDF 0.45μm membranes (BioRad ...