Labshake search
Citations for Bio-Rad :
8951 - 9000 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 40 μg proteins were loaded and separated by SDS-PAGE gels (10%) electrophoretically and transferred to polyvinylidene difluoride membrane (Bio-Rad Incorporation, 10026933). Blocking was performed by adding 5% BSA for 60 min at room temperature on the shaker ...
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bioRxiv - Molecular Biology 2021Quote: ... The boiled proteins were separated on 4% to 15% SDS-PAGE gradient gels and transferred to LF PVDF membrane (Bio-Rad, Cat #1620260). The membranes were blocked in 5% skim milk in TBST (20 mM-Tris ...
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bioRxiv - Cell Biology 2022Quote: ... the intestine-specific intestinal fatty acid binding protein promoter (-2.3kb fabp2 -also called ifabp-) was amplified by high fidelity PCR (iProof_ High-Fidelity DNA Polymerase; Bio-Rad, Hercules, CA, USA) from p5E–2.3ifabp plasmid (kindly gifted by J ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were centrifuged at 16,000 x g to pellet cellular debris and protein concentration was determined by BCA quantification assay (Bio-Rad, Hercules, CA, USA). For each sample ...
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bioRxiv - Cell Biology 2019Quote: ... For each sample 50 μg of protein were incubated at 95°C for 10 min and then loaded into a gradient (4-15%) western blot gel (mini-protein TGX gel, Bio-Rad, Milan, Italy). Proteins were transferred onto a 0.45 μm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biophysics 2019Quote: ... was performed before staining the gel with Coomassie Blue and imaging overall protein content using a ChemiDoc MP instrument (Bio-Rad, Hercules, CA).
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bioRxiv - Microbiology 2019Quote: ... 60 μg of protein extract for each strain analyzed was loaded into a 10% TGX Stain-Free™ FastCast™ Acrylamide gel (Bio-Rad). Separated proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ transfer system (Bio-Rad) ...
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bioRxiv - Biophysics 2019Quote: ... The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad, Hercules, California). In all purification steps ...
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bioRxiv - Plant Biology 2019Quote: Nectars and the protein extracts were separated by stain-free 1D SDS-PAGE (Bio-Rad’s Mini PROTEAN® TGX Stain-Free™ Gels). For western blot assays ...
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bioRxiv - Cancer Biology 2019Quote: ... CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories, Inc.) and imaged on ChemiDoc MP (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2019Quote: ... Gels were run at 150 V for 1 hour and the protein were subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad, Hercules, CA, USA). All membranes were stained with Ponceau as a qualitative check for equivalent of total protein loading and transfer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein separation was done by 1D SDS-PAGE electrophoresis using commercial gels to ensure reproducibility (8–16% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad). A sample of venom of ISm and ISy lines ...
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bioRxiv - Cancer Biology 2020Quote: Proteins from WCL samples were separated on 4-20% (w/v) Tris-Glycine SDS-PAGE gels (Bio-Rad; Cat#456-1094, Hercules, CA). We loaded 30-50 μg protein per sample ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µg of each protein sample was separated in one dimension using 4-20% tris-glycine gels (Mini-PROTEAN TGX, Bio-Rad Laboratories, Inc.). A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are listed in Supplemental table 2 Immunoreactive proteins were visualized with Clarity Western ECL Substrate (Bio-Rad Laboratories, Hercules, CA, USA). The UVP ChemStudio (Analytik Jena US LLC ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred from the gel onto a 0.2 µm nitrocellulose membrane using the wet transfer method (Bio-Rad Laboratories, Hercules, CA). Overnight transfer was performed for 16 h using a constant voltage of 30V ...
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bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE (90 min at 150V) using 4-20% Criterion™ TGX Stain-Free™ gel (Bio-Rad, 5678095) and electro-blotted onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were separated by SDS-PAGE and blotted onto a PVDF membrane as recommended by the manufacturer (Transblot, Bio-Rad Laboratories, USA). The membrane was blocked overnight at 4 °C in a solution of 5 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... and phospho-Akt (p-Akt) were quantified in 10 μg of total protein using the Bio-Plex Pro magnetic phosphoprotein detection assay (Bio-Rad, United Kingdom). Phosphoprotein expression was normalized to β-actin and expressed as fold-change relative to the uninfected (UI ...
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bioRxiv - Microbiology 2021Quote: ... 20 µL sample was loaded per each lane of an any KD Mini-PROTEAN® TGX™ Precast Protein Gel (BioRad Hercules, CA) and run at 150 V for 1 hour ...
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bioRxiv - Bioengineering 2019Quote: ... Proteins (15 μg each) were separated based on their molecular weight with SDS–PAGE on 10% Mini-PROTEAN gels (Bio-Rad, Hercules, CA) and then transferred to PVDF membranes (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Equal total protein amounts for each sample were used in co-immunoprecipitation assays and analyzed by SDS-PAGE on Criterion gels (Bio-Rad, Hercules, CA). Co-immunoprecipitation assays were performed as described (49 ...
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bioRxiv - Systems Biology 2022Quote: ... sample buffer (5 minutes at 95°C) and subjected to gel electrophoresis (4– 15% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and immunoblotting (polyvinylidene difluoride Transfer Membrane ...
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bioRxiv - Neuroscience 2022Quote: ... The nascent and total protein samples were then run on a 4-12% gradient SDS-polyacrylamide gel along with Precision Plus Protein Kaleidoscope ladder (Bio-Rad, Cat #1610395). Then the proteins were transferred onto nitrocellulose membranes using either a Bio-Rad Trans-Blot Turbo Transfer system (25V ...
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bioRxiv - Biophysics 2022Quote: The purified proteins of V2HeR3 were reconstituted into a mixture of POPE and POPG membranes (molar ratio = 3:1) with a protein-to-lipid molar ratio of 1:20 by removing DDM using Bio-Beads (SM-2; Bio-Rad, CA, USA). The reconstituted samples were washed three times with 1 mM NaCl and 2 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Immunology 2021Quote: ... Basically 300 ug of IVIG protein were subjected to isoelectric focusing (IPG) across pH gradients of 4-7 (Biorad, LifeSciences, Hercules, Ca, USA) or pH 6-11 (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to nitrocellulose membrane by semi-dry transfer using the Trans-Blot Turbo Transfer System (BioRad, 1.5 A for 7 min). Membranes were blocked in 5% milk in 1x PBS with 0.1% Tween 20 (PBST ...
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bioRxiv - Physiology 2021Quote: ... Protein was run on Criterion TGX Stain-Free Precast Gels and total protein visualized using the ChemiDoc MP Imaging System (Bio-Rad, Watford, UK). Bands were visualized with Bio-Rad Clarity Western ECL (Watford ...
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bioRxiv - Neuroscience 2021Quote: Protein lysates from SH-SY5Y cells were quantified (bicinchoninic acid protein assay, Pierce) and then electrophoresed in 4—12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad) in 1X TGS Tris/Glycine/SDS Buffer (10X TGS Buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels (BioRad, Hercules, CA, Cat#17000435). In addition ...
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bioRxiv - Bioengineering 2021Quote: Protein (50 μg) was separated by SDS□ polyacrylamide gel electrophoresis and transferred onto PVDF (polyvinylidene fluoride) membrane (Bio-Rad Lab Inc., Hercules, CA) by electro blotting ...
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bioRxiv - Neuroscience 2022Quote: ... chemiluminescent imaging of target proteins was performed with Immobilon Forte Western HRP Substrate (EMD Millpore, Billerica, MA) using the ChemiDoc Imaging Systems (BioRad Laboratories, Hercules, CA). Band density of oxytocin receptor was quantified using Image Lab 6.0 (BioRad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 90°C and loaded on a 4–20% (w/v) Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were separated by electrophoresis and blotted onto a membrane with trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs (Bio-Rad) ...
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bioRxiv - Neuroscience 2019Quote: ... or POPC:palmitoyloleoylphosphatidylserine (POPS (85:15) at a 1:200 protein to lipid ratio by dialysis in the presence of Bio-Beads (Bio-Rad, Hercules, CA) into metal free buffer ...
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bioRxiv - Pathology 2019Quote: ... this amount of protein was broken down via a precipitation and purification kit (ReadyPrep™ 2-D Cleanup Kit, Bio-Rad, Warsaw, Poland). The obtained protein pellets were then dissolved in a rehydration buffer ...
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bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
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bioRxiv - Microbiology 2019Quote: ... The values were normalized according to the total protein quantified by Bradford using the Quick Start™ Bradford reagent (BioRad, Hercules, CA, USA). Values were expressed as the relative GFP fluorescence intensity ...
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bioRxiv - Cell Biology 2020Quote: Purified recombinant proteins and protein extracts prepared from harvested cells or immunoprecipitation experiments in reducing Laemmli Sample Buffer were run on 4–20 % Mini-PROTEAN® TGX™ Precast Protein Gels of different comb and well-sizes (Bio-Rad, #456-1093 ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein was measured from the 50 μL sample of homogenate (described above) using a Quick Start Bradford assay (BioRad, Cat. Number 5000205). Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant containing approximately 15-30 μg protein was separated by SDS-PAGE using a 4-20% gradient Mini-PROTEAN TGX Precast Gel (Bio-Rad, Hercules, CA) and then transferred to a 0.2 μM nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on a 10% SDS polyacrylamide gel and visualised with Silver Stain Plus Kit (Bio-Rad Laboratories AB, Sweden) according to manufacturer’s modified silver stain protocol.
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bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
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bioRxiv - Microbiology 2021Quote: ... Proteins were quantified by the Bradford method and 20 to 30 μg of total protein extract were loaded on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). After transfer onto nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2019Quote: ... and equal amounts of total protein (10 to 20 µg) were loaded on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad). All samples for a participant were loaded in adjacent lanes on the same gel ...
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bioRxiv - Synthetic Biology 2019Quote: ... The protein pellet was resuspended in 100 mM ammonium bicarbonate (AMBIC) with 20% methanol and quantified by the Lowry method (Bio-Rad DC assay). A total of 100 µg of protein was reduced by adding tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were normalized for protein concentration using a BCA assay kit (Thermo 23225) and mixed with Laemmli buffer (Bio-Rad 161-0737). 50 µg of the protein mix were run through a 4-20% Criterion TGX gel (Bio-Rad 5671093 ...
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bioRxiv - Microbiology 2020Quote: ... the outer membrane control proteins OmpA (α-OmpA was kindly provided by Prasadaro Nemani (45)) or MOMP (α-MOMP, MONOFLUO™ Legionella pneumophila IFA Test Kit, BioRad), the inner membrane control protein LepB (antibody kindly provided by Gunnar von Heinje (46) ...